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Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP13_F_L14
         (780 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||...    35   0.011
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1...    34   0.020
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit...    29   0.75 
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch...    27   2.3  
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac...    27   3.0  
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe...    27   3.0  

>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1461

 Score = 35.1 bits (77), Expect = 0.011
 Identities = 14/36 (38%), Positives = 15/36 (41%)
 Frame = +2

Query: 620 PPPXXFXXQPPPPPPXXXXGXPAPXXPPXXXPXLXA 727
           PPP      PPPPPP        P  PP   P + A
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSA 787



 Score = 33.5 bits (73), Expect = 0.035
 Identities = 22/69 (31%), Positives = 22/69 (31%)
 Frame = +1

Query: 499 PPPXXPXPXGXXXXFXXPPPGGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXG 678
           PPP    P         PPP        PP    G  G  PP      P  PPPP    G
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPP------PPPPPPPAVSAG 788

Query: 679 XXRXXXPPP 705
             R   P P
Sbjct: 789 GSRYYAPAP 797



 Score = 29.1 bits (62), Expect = 0.75
 Identities = 16/51 (31%), Positives = 17/51 (33%)
 Frame = +2

Query: 512 PPXPXGXXXXXXXXXXXXXXKPXXPLFFXGGGGGXXPPPXXFXXQPPPPPP 664
           PP P                 P  P+   GG     PPP      PPPPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIM--GGPPPPPPPPGVAGAGPPPPPP 780



 Score = 28.3 bits (60), Expect = 1.3
 Identities = 14/44 (31%), Positives = 14/44 (31%)
 Frame = +2

Query: 647 PPPPPPXXXXGXPAPXXPPXXXPXLXAXXXXXXXXPXXXXXGGP 778
           PPPPPP      PAP   P   P            P      GP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP 775



 Score = 27.5 bits (58), Expect = 2.3
 Identities = 13/48 (27%), Positives = 14/48 (29%)
 Frame = +1

Query: 619 PPPXXXXXPTXPPPPXXXXGXXRXXXPPPXXXXSXXXXVXXPXPPXXP 762
           P P     P  PPPP           PPP    +       P P   P
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEP 801



 Score = 27.1 bits (57), Expect = 3.0
 Identities = 11/23 (47%), Positives = 11/23 (47%)
 Frame = -2

Query: 626 GGGXXPPPPPXKRGGXXVFXPPP 558
           GG   PPPPP   G      PPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPP 781


>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 574

 Score = 34.3 bits (75), Expect = 0.020
 Identities = 22/88 (25%), Positives = 23/88 (26%)
 Frame = +1

Query: 499 PPPXXPXPXGXXXXFXXPPPGGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXG 678
           PPP  P           PP G       PP      G   PP       + PP P     
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396

Query: 679 XXRXXXPPPXXXXSXXXXVXXPXPPXXP 762
                  PP    S       P PP  P
Sbjct: 397 GRSAPALPPLGNASRTSTPPVPTPPSLP 424



 Score = 25.4 bits (53), Expect = 9.2
 Identities = 13/38 (34%), Positives = 14/38 (36%)
 Frame = +1

Query: 550 PPPGGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPP 663
           PPP        PP+  G      PPP        PPPP
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPP--------PPPP 343


>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
            hand and WH2 motif |Schizosaccharomyces pombe|chr
            1|||Manual
          Length = 1794

 Score = 29.1 bits (62), Expect = 0.75
 Identities = 11/28 (39%), Positives = 12/28 (42%)
 Frame = +2

Query: 620  PPPXXFXXQPPPPPPXXXXGXPAPXXPP 703
            PPP      PPP  P    G P+   PP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734



 Score = 27.5 bits (58), Expect = 2.3
 Identities = 13/34 (38%), Positives = 13/34 (38%), Gaps = 3/34 (8%)
 Frame = +2

Query: 623  PPXXFXXQPPPPP---PXXXXGXPAPXXPPXXXP 715
            PP      PPPPP   P      P P  PP   P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732


>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
           Y|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 1002

 Score = 27.5 bits (58), Expect = 2.3
 Identities = 27/113 (23%), Positives = 29/113 (25%), Gaps = 3/113 (2%)
 Frame = +1

Query: 376 PPPXFXGEKXXGXXPPFFPPXRXXXXXXXXXXXXXKKXXFSPPPXXPXPXGXXXXFXXPP 555
           PPP    E      PP  P                +     PPP      G       PP
Sbjct: 221 PPPPMHHEPGEHMPPP--PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP---PP 275

Query: 556 P---GGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXGXXRXXXPPP 705
           P     G     PP+    G    PPP         PPP           PPP
Sbjct: 276 PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 328



 Score = 27.5 bits (58), Expect = 2.3
 Identities = 27/113 (23%), Positives = 29/113 (25%), Gaps = 3/113 (2%)
 Frame = +1

Query: 376 PPPXFXGEKXXGXXPPFFPPXRXXXXXXXXXXXXXKKXXFSPPPXXPXPXGXXXXFXXPP 555
           PPP    E      PP  P                +     PPP      G       PP
Sbjct: 234 PPPPMHHEPGEHMPPP--PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP---PP 288

Query: 556 P---GGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXGXXRXXXPPP 705
           P     G     PP+    G    PPP         PPP           PPP
Sbjct: 289 PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 341



 Score = 26.2 bits (55), Expect = 5.3
 Identities = 20/72 (27%), Positives = 21/72 (29%), Gaps = 3/72 (4%)
 Frame = +1

Query: 499 PPPXXPXPXGXXXXFXXPPP---GGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXX 669
           PPP    P         PPP     G     PP+    G    PPP         PPP  
Sbjct: 209 PPPMHHKPG----EHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPM 264

Query: 670 XXGXXRXXXPPP 705
                    PPP
Sbjct: 265 HHEPGEHMPPPP 276


>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
           Spp42|Schizosaccharomyces pombe|chr 1|||Manual
          Length = 2363

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 11/17 (64%), Positives = 11/17 (64%), Gaps = 2/17 (11%)
 Frame = +2

Query: 620 PPPXXFXX--QPPPPPP 664
           PPP  F    QPPPPPP
Sbjct: 13  PPPPGFEPPSQPPPPPP 29



 Score = 25.8 bits (54), Expect = 7.0
 Identities = 9/19 (47%), Positives = 10/19 (52%)
 Frame = +2

Query: 647 PPPPPPXXXXGXPAPXXPP 703
           PPPPPP      P+   PP
Sbjct: 9   PPPPPPPPGFEPPSQPPPP 27



 Score = 25.4 bits (53), Expect = 9.2
 Identities = 9/19 (47%), Positives = 9/19 (47%)
 Frame = +2

Query: 647 PPPPPPXXXXGXPAPXXPP 703
           PPPPP       P P  PP
Sbjct: 12  PPPPPGFEPPSQPPPPPPP 30


>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 273

 Score = 27.1 bits (57), Expect = 3.0
 Identities = 22/70 (31%), Positives = 22/70 (31%), Gaps = 1/70 (1%)
 Frame = -2

Query: 704 GGGXXXRXXPXXSXGGGGXVGXXXXXGG-GXXPPPPPXKRGGXXVFXPPPGGGXXKXXXX 528
           GG       P    G GG  G     GG G     P    GG   F   PGG        
Sbjct: 195 GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGF 254

Query: 527 PXGXGXKGGG 498
             G G  GGG
Sbjct: 255 GGGPGGFGGG 264



 Score = 26.6 bits (56), Expect = 4.0
 Identities = 19/55 (34%), Positives = 20/55 (36%)
 Frame = -1

Query: 663 GGGGGGWXXXXXGGGXXPPPPPXKKRGXXGFXXXXXXXXPKXXXXPXGXGGXRGG 499
           GGG GG+     GG   PPP P    G  GF                G GG  GG
Sbjct: 187 GGGFGGFG----GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFG-GGPGGFEGG 236


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,321,589
Number of Sequences: 5004
Number of extensions: 39980
Number of successful extensions: 249
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)

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