BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_L14
(780 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 35 0.011
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 34 0.020
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 29 0.75
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 27 2.3
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 3.0
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 3.0
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 35.1 bits (77), Expect = 0.011
Identities = 14/36 (38%), Positives = 15/36 (41%)
Frame = +2
Query: 620 PPPXXFXXQPPPPPPXXXXGXPAPXXPPXXXPXLXA 727
PPP PPPPPP P PP P + A
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSA 787
Score = 33.5 bits (73), Expect = 0.035
Identities = 22/69 (31%), Positives = 22/69 (31%)
Frame = +1
Query: 499 PPPXXPXPXGXXXXFXXPPPGGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXG 678
PPP P PPP PP G G PP P PPPP G
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPP------PPPPPPPAVSAG 788
Query: 679 XXRXXXPPP 705
R P P
Sbjct: 789 GSRYYAPAP 797
Score = 29.1 bits (62), Expect = 0.75
Identities = 16/51 (31%), Positives = 17/51 (33%)
Frame = +2
Query: 512 PPXPXGXXXXXXXXXXXXXXKPXXPLFFXGGGGGXXPPPXXFXXQPPPPPP 664
PP P P P+ GG PPP PPPPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIM--GGPPPPPPPPGVAGAGPPPPPP 780
Score = 28.3 bits (60), Expect = 1.3
Identities = 14/44 (31%), Positives = 14/44 (31%)
Frame = +2
Query: 647 PPPPPPXXXXGXPAPXXPPXXXPXLXAXXXXXXXXPXXXXXGGP 778
PPPPPP PAP P P P GP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP 775
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/48 (27%), Positives = 14/48 (29%)
Frame = +1
Query: 619 PPPXXXXXPTXPPPPXXXXGXXRXXXPPPXXXXSXXXXVXXPXPPXXP 762
P P P PPPP PPP + P P P
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEP 801
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = -2
Query: 626 GGGXXPPPPPXKRGGXXVFXPPP 558
GG PPPPP G PPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPPP 781
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 34.3 bits (75), Expect = 0.020
Identities = 22/88 (25%), Positives = 23/88 (26%)
Frame = +1
Query: 499 PPPXXPXPXGXXXXFXXPPPGGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXG 678
PPP P PP G PP G PP + PP P
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIP 396
Query: 679 XXRXXXPPPXXXXSXXXXVXXPXPPXXP 762
PP S P PP P
Sbjct: 397 GRSAPALPPLGNASRTSTPPVPTPPSLP 424
Score = 25.4 bits (53), Expect = 9.2
Identities = 13/38 (34%), Positives = 14/38 (36%)
Frame = +1
Query: 550 PPPGGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPP 663
PPP PP+ G PPP PPPP
Sbjct: 314 PPPPSRRNRGKPPIGNGSSNSSLPPP--------PPPP 343
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 29.1 bits (62), Expect = 0.75
Identities = 11/28 (39%), Positives = 12/28 (42%)
Frame = +2
Query: 620 PPPXXFXXQPPPPPPXXXXGXPAPXXPP 703
PPP PPP P G P+ PP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 27.5 bits (58), Expect = 2.3
Identities = 13/34 (38%), Positives = 13/34 (38%), Gaps = 3/34 (8%)
Frame = +2
Query: 623 PPXXFXXQPPPPP---PXXXXGXPAPXXPPXXXP 715
PP PPPPP P P P PP P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 27.5 bits (58), Expect = 2.3
Identities = 27/113 (23%), Positives = 29/113 (25%), Gaps = 3/113 (2%)
Frame = +1
Query: 376 PPPXFXGEKXXGXXPPFFPPXRXXXXXXXXXXXXXKKXXFSPPPXXPXPXGXXXXFXXPP 555
PPP E PP P + PPP G PP
Sbjct: 221 PPPPMHHEPGEHMPPP--PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP---PP 275
Query: 556 P---GGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXGXXRXXXPPP 705
P G PP+ G PPP PPP PPP
Sbjct: 276 PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 328
Score = 27.5 bits (58), Expect = 2.3
Identities = 27/113 (23%), Positives = 29/113 (25%), Gaps = 3/113 (2%)
Frame = +1
Query: 376 PPPXFXGEKXXGXXPPFFPPXRXXXXXXXXXXXXXKKXXFSPPPXXPXPXGXXXXFXXPP 555
PPP E PP P + PPP G PP
Sbjct: 234 PPPPMHHEPGEHMPPP--PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMP---PP 288
Query: 556 P---GGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXXXXGXXRXXXPPP 705
P G PP+ G PPP PPP PPP
Sbjct: 289 PMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 341
Score = 26.2 bits (55), Expect = 5.3
Identities = 20/72 (27%), Positives = 21/72 (29%), Gaps = 3/72 (4%)
Frame = +1
Query: 499 PPPXXPXPXGXXXXFXXPPP---GGGXKTXXPPLFXGGGGGXXPPPXXXXXPTXPPPPXX 669
PPP P PPP G PP+ G PPP PPP
Sbjct: 209 PPPMHHKPG----EHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPMHHEPGEHMPPPPM 264
Query: 670 XXGXXRXXXPPP 705
PPP
Sbjct: 265 HHEPGEHMPPPP 276
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.1 bits (57), Expect = 3.0
Identities = 11/17 (64%), Positives = 11/17 (64%), Gaps = 2/17 (11%)
Frame = +2
Query: 620 PPPXXFXX--QPPPPPP 664
PPP F QPPPPPP
Sbjct: 13 PPPPGFEPPSQPPPPPP 29
Score = 25.8 bits (54), Expect = 7.0
Identities = 9/19 (47%), Positives = 10/19 (52%)
Frame = +2
Query: 647 PPPPPPXXXXGXPAPXXPP 703
PPPPPP P+ PP
Sbjct: 9 PPPPPPPPGFEPPSQPPPP 27
Score = 25.4 bits (53), Expect = 9.2
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +2
Query: 647 PPPPPPXXXXGXPAPXXPP 703
PPPPP P P PP
Sbjct: 12 PPPPPGFEPPSQPPPPPPP 30
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.1 bits (57), Expect = 3.0
Identities = 22/70 (31%), Positives = 22/70 (31%), Gaps = 1/70 (1%)
Frame = -2
Query: 704 GGGXXXRXXPXXSXGGGGXVGXXXXXGG-GXXPPPPPXKRGGXXVFXPPPGGGXXKXXXX 528
GG P G GG G GG G P GG F PGG
Sbjct: 195 GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGF 254
Query: 527 PXGXGXKGGG 498
G G GGG
Sbjct: 255 GGGPGGFGGG 264
Score = 26.6 bits (56), Expect = 4.0
Identities = 19/55 (34%), Positives = 20/55 (36%)
Frame = -1
Query: 663 GGGGGGWXXXXXGGGXXPPPPPXKKRGXXGFXXXXXXXXPKXXXXPXGXGGXRGG 499
GGG GG+ GG PPP P G GF G GG GG
Sbjct: 187 GGGFGGFG----GGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFG-GGPGGFEGG 236
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,321,589
Number of Sequences: 5004
Number of extensions: 39980
Number of successful extensions: 249
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 52
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 142
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 377352472
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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