BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_K02
(815 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 32 0.085
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.20
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.8
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 27 2.4
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 3.2
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 4.2
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 32.3 bits (70), Expect = 0.085
Identities = 15/37 (40%), Positives = 16/37 (43%)
Frame = +2
Query: 359 PPPTXTXKXXXPPLATXPPXRXARXPXPPPPXPPXLS 469
PPP PP PP P PPPP PP +S
Sbjct: 752 PPPAPIMGGPPPP--PPPPGVAGAGPPPPPPPPPAVS 786
Score = 29.5 bits (63), Expect = 0.60
Identities = 13/41 (31%), Positives = 15/41 (36%)
Frame = +2
Query: 359 PPPTXTXKXXXPPLATXPPXRXARXPXPPPPXPPXLSFXXP 481
PPP P PP PPPP PP ++ P
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGP 775
Score = 28.3 bits (60), Expect = 1.4
Identities = 11/30 (36%), Positives = 12/30 (40%)
Frame = +3
Query: 330 PPPXLXXXXTPPPPQPXKXXXPPXPPXXXP 419
P P + PPPP PP PP P
Sbjct: 754 PAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 27.9 bits (59), Expect = 1.8
Identities = 11/30 (36%), Positives = 12/30 (40%)
Frame = +3
Query: 330 PPPXLXXXXTPPPPQPXKXXXPPXPPXXXP 419
PP + PPPP P P PP P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.5 bits (58), Expect = 2.4
Identities = 12/32 (37%), Positives = 12/32 (37%)
Frame = +1
Query: 325 PPPXPXXWXXKPPPHXNPKXXKXPPXHPPXXP 420
PPP P PPP PP PP P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 26.2 bits (55), Expect = 5.6
Identities = 11/28 (39%), Positives = 11/28 (39%)
Frame = +3
Query: 720 PXXNNPXTXXXXPVPAXXXPPPPNXPPP 803
P P P A PPPP PPP
Sbjct: 756 PIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 31.1 bits (67), Expect = 0.20
Identities = 30/114 (26%), Positives = 34/114 (29%), Gaps = 3/114 (2%)
Frame = +2
Query: 149 PPPLPPXXXXKKXSXFXPPPXXXXKXXKXRXXGXXPXXGGXVRXPPXXKKXXXXGGGXXX 328
PPP PP S PP P G R PP
Sbjct: 337 PPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSA--PSTG---RQPPPLSSSRAVSN-PPA 390
Query: 329 PPXXLXGXXNP--PPTXTX-KXXXPPLATXPPXRXARXPXPPPPXPPXLSFXXP 481
PP + G P PP + PP+ T P + P PP PP L P
Sbjct: 391 PPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAP 444
Score = 25.4 bits (53), Expect = 9.7
Identities = 13/43 (30%), Positives = 13/43 (30%)
Frame = +2
Query: 329 PPXXLXGXXNPPPTXTXKXXXPPLATXPPXRXARXPXPPPPXP 457
PP G PP PPL P P P P P
Sbjct: 436 PPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 1.8
Identities = 11/34 (32%), Positives = 13/34 (38%)
Frame = +2
Query: 359 PPPTXTXKXXXPPLATXPPXRXARXPXPPPPXPP 460
PP PP++ PP P PP PP
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPP 1732
Score = 25.8 bits (54), Expect = 7.4
Identities = 11/31 (35%), Positives = 13/31 (41%)
Frame = +2
Query: 359 PPPTXTXKXXXPPLATXPPXRXARXPXPPPP 451
PPP+ PP A PP + P P P
Sbjct: 1716 PPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
Score = 25.4 bits (53), Expect = 9.7
Identities = 13/42 (30%), Positives = 16/42 (38%)
Frame = +2
Query: 332 PXXLXGXXNPPPTXTXKXXXPPLATXPPXRXARXPXPPPPXP 457
P + PPP + PP + PP PPPP P
Sbjct: 1699 PPQMSAPTPPPPPMSV----PPPPSAPPMPAGPPSAPPPPLP 1736
Score = 25.4 bits (53), Expect = 9.7
Identities = 11/32 (34%), Positives = 11/32 (34%)
Frame = +1
Query: 325 PPPXPXXWXXKPPPHXNPKXXKXPPXHPPXXP 420
P P P PPP P P PP P
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 27.5 bits (58), Expect = 2.4
Identities = 13/25 (52%), Positives = 13/25 (52%)
Frame = -3
Query: 465 RXGGXGGGGXGXRAXRXGGXVARGG 391
R G GGG G R R G ARGG
Sbjct: 32 RGGARGGGRGGARGGRGGRGGARGG 56
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.2
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +3
Query: 756 PVPAXXXPPPPNXPP 800
P+PA PPPP PP
Sbjct: 234 PLPAPPPPPPPTLPP 248
Score = 26.2 bits (55), Expect = 5.6
Identities = 12/35 (34%), Positives = 15/35 (42%)
Frame = +2
Query: 356 NPPPTXTXKXXXPPLATXPPXRXARXPXPPPPXPP 460
+PPP+ P + P P PPPP PP
Sbjct: 166 SPPPSFQPPSAAAPATSLPSDYN---PPPPPPPPP 197
Score = 26.2 bits (55), Expect = 5.6
Identities = 12/25 (48%), Positives = 13/25 (52%)
Frame = +2
Query: 392 PPLATXPPXRXARXPXPPPPXPPXL 466
PP T P + P PPPP PP L
Sbjct: 224 PP--TYTPKQADPLPAPPPPPPPTL 246
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.6 bits (56), Expect = 4.2
Identities = 10/29 (34%), Positives = 14/29 (48%)
Frame = +2
Query: 359 PPPTXTXKXXXPPLATXPPXRXARXPXPP 445
PPPT + P +A+ PP + PP
Sbjct: 910 PPPTASMTASAPAIASPPPPKVGETYHPP 938
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.317 0.152 0.560
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,575,542
Number of Sequences: 5004
Number of extensions: 20725
Number of successful extensions: 155
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 35
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 122
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 398435810
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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