Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_J09
(848 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC926.04c |hsp90|swo1|heat shock protein Hsp90|Schizosaccharom... 143 3e-35
SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein homolog|Schi... 32 0.089
SPBC14F5.05c |sam1||S-adenosylmethionine synthetase |Schizosacch... 27 3.4
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 26 5.9
SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27 family|Schi... 26 5.9
SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit |Schizosacc... 26 7.8
SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|c... 26 7.8
SPAC19A8.14 |||aminoacyl-tRNA hydrolase |Schizosaccharomyces pom... 26 7.8
>SPAC926.04c |hsp90|swo1|heat shock protein
Hsp90|Schizosaccharomyces pombe|chr 1|||Manual
Length = 704
Score = 143 bits (346), Expect = 3e-35
Identities = 74/115 (64%), Positives = 87/115 (75%), Gaps = 2/115 (1%)
Frame = +2
Query: 446 RXDLVNNLGTIAKSGTXAFMEALQAGADISMIGQFGVGFYSSYLVADRVTVHSKHNDDEQ 625
+ DL+NNLG IAKSGT FMEA +GADISMIGQFGVGFYS+YLVAD+V V SKHNDDEQ
Sbjct: 87 KNDLINNLGVIAKSGTKQFMEAAASGADISMIGQFGVGFYSAYLVADKVQVVSKHNDDEQ 146
Query: 626 YVWESSAGGSFTVRPDSGEP-LGRGTKIVLHVKEDWXXHGRTXNQRDR-KEXSQF 784
Y+WESSAGGSFTV D+ P L RGT+I L +KED + +D K+ S+F
Sbjct: 147 YIWESSAGGSFTVTLDTDGPRLLRGTEIRLFMKEDQLQYLEEKTIKDTVKKHSEF 201
Score = 102 bits (245), Expect = 6e-23
Identities = 49/68 (72%), Positives = 58/68 (85%)
Frame = +1
Query: 244 IINTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELYIKIIPNKNEGTLTII 423
IINT YSNKEIFLRELISN+SDALDKIRY+SL+DP LD+ K+L+I+I P+K L+I
Sbjct: 20 IINTVYSNKEIFLRELISNASDALDKIRYQSLSDPHALDAEKDLFIRITPDKENKILSIR 79
Query: 424 DTGIGMTK 447
DTGIGMTK
Sbjct: 80 DTGIGMTK 87
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/18 (66%), Positives = 15/18 (83%)
Frame = +3
Query: 189 AEVETFAFQAEIAQLMSL 242
+ ETF F+AEI+QLMSL
Sbjct: 2 SNTETFKFEAEISQLMSL 19
>SPBC21C3.01c |vps13a|vps1301, SPBC31F10.18c|chorein
homolog|Schizosaccharomyces pombe|chr 2|||Manual
Length = 3071
Score = 32.3 bits (70), Expect = 0.089
Identities = 24/88 (27%), Positives = 41/88 (46%), Gaps = 6/88 (6%)
Frame = +1
Query: 262 SNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELYIKIIPNKNEGTLTIIDTGIGM 441
+N + R++++N L KI DPSK ++Y K+ + + L D+GI +
Sbjct: 2448 NNTKFISRDIVANG---LSKILILKDYDPSKAVRKPKIYSKVSTEERDFNLEQFDSGIDL 2504
Query: 442 TKXRFGEQFG------NHREIWYXSFHG 507
+ E G N +E+ Y +FHG
Sbjct: 2505 SVKFLLEGIGISLVERNTQELAYLTFHG 2532
Score = 27.5 bits (58), Expect = 2.5
Identities = 13/46 (28%), Positives = 23/46 (50%)
Frame = +1
Query: 247 INTFYSNKEIFLRELISNSSDALDKIRYESLTDPSKLDSGKELYIK 384
+N S +EI L++ I + D I+Y S S ++ +Y+K
Sbjct: 377 LNEQISKEEIDLQKKIEKRNSTYDLIKYRSRVHTSLIEERNSIYLK 422
>SPBC14F5.05c |sam1||S-adenosylmethionine synthetase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 382
Score = 27.1 bits (57), Expect = 3.4
Identities = 14/45 (31%), Positives = 19/45 (42%)
Frame = +2
Query: 608 HNDDEQYVWESSAGGSFTVRPDSGEPLGRGTKIVLHVKEDWXXHG 742
H DE+ V+ G F V G+ G KI++ W HG
Sbjct: 221 HLLDEKTVYHIQPSGRFVVGGPQGDAGLTGRKIIVDTYGGWGAHG 265
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 26.2 bits (55), Expect = 5.9
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = -3
Query: 399 LVGNDLDVQLFATIEF*RIRERFIPDFV*SVR*IGNQLTKENFFV 265
LV + LDV F + ++ F+ D+ SVR N+ K NFF+
Sbjct: 295 LVISMLDVDGFRIDKATQMTVDFLVDWAKSVRLCANRFNKSNFFI 339
>SPAC19B12.01 ||SPAC4F10.21|TPR repeat protein, TTC27
family|Schizosaccharomyces pombe|chr 1|||Manual
Length = 817
Score = 26.2 bits (55), Expect = 5.9
Identities = 8/26 (30%), Positives = 15/26 (57%)
Frame = +3
Query: 381 QDHSQQERGHSYDHRYRYWYDQGPIW 458
+DH++++ H+ +Y YD IW
Sbjct: 603 KDHTKEQAWHAMQQGIKYMYDNWRIW 628
>SPBC146.14c |sec26|SPBC337.01c|coatomer beta subunit
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 940
Score = 25.8 bits (54), Expect = 7.8
Identities = 8/21 (38%), Positives = 17/21 (80%)
Frame = +1
Query: 277 FLRELISNSSDALDKIRYESL 339
F+R+++ +D L+K+R++SL
Sbjct: 224 FIRKVVLTKADGLEKLRFQSL 244
>SPCC757.12 |||alpha-amylase homolog |Schizosaccharomyces pombe|chr
3|||Manual
Length = 625
Score = 25.8 bits (54), Expect = 7.8
Identities = 12/35 (34%), Positives = 20/35 (57%)
Frame = +2
Query: 149 SSKENAGRNGDTASGG*NLRVPG*DRSAYVPGSST 253
SS ++ R+G T+S G + + +S + GSST
Sbjct: 543 SSSSSSSRSGSTSSSGSSHTITSTSQSVHTSGSST 577
>SPAC19A8.14 |||aminoacyl-tRNA hydrolase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 205
Score = 25.8 bits (54), Expect = 7.8
Identities = 10/28 (35%), Positives = 16/28 (57%)
Frame = -2
Query: 529 VGTCLKSLHESXSTRFRDGSQIVHQIGP 446
+G C + +H++ T+ GS V IGP
Sbjct: 162 LGLCARVIHDAGRTQIASGSATVLGIGP 189
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,297,907
Number of Sequences: 5004
Number of extensions: 67154
Number of successful extensions: 190
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 180
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 189
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 420459900
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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