Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_J05
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal pro... 312 4e-86
SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein S4|Schizos... 311 1e-85
SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein S4|Schizosacch... 310 1e-85
SPCC1450.11c |cek1||serine/threonine protein kinase Cek1|Schizos... 27 2.7
SPBC18H10.10c |cwc16||complexed with Cdc5 protein Cwf16|Schizosa... 27 2.7
SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces pomb... 27 4.7
SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces ... 27 4.7
SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces p... 26 6.2
>SPBC19F8.08 |rps401|rps4-1, rps4, SPBC25H2.17c|40S ribosomal
protein S4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 262
Score = 312 bits (766), Expect = 4e-86
Identities = 140/193 (72%), Positives = 161/193 (83%)
Frame = +1
Query: 64 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 243
M RGPKKHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1 MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60
Query: 244 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 423
V I+ QRLIKVDGKVRTD T+P GFMDV+S+EKT E FRL+YD+KGRFT+HRIT EEAK
Sbjct: 61 VKAILMQRLIKVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAK 120
Query: 424 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNL 603
YKLCKVKRV G K VP+LVTHDGRTIRYPDPLIKVND+I+L++ T KI FIKF++
Sbjct: 121 YKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQ 180
Query: 604 CMITGGRNLGACG 642
M+TGGRN+G G
Sbjct: 181 VMVTGGRNMGRVG 193
Score = 48.8 bits (111), Expect = 1e-06
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +2
Query: 632 GRVGTIVXRERHPGSFDIVHIKDSTGHTFATKV 730
GRVGTIV RE H GSF+I+H+KD+ FAT++
Sbjct: 190 GRVGTIVHREHHLGSFEIIHVKDALDREFATRL 222
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/36 (55%), Positives = 30/36 (83%)
Frame = +3
Query: 729 LNNVFIIGKGTXAYISLPRGKGIRLTIAEXRDKRIA 836
L+NVF+IG+ ++ISLP+GKG++L+I E RD+R A
Sbjct: 222 LSNVFVIGETGKSWISLPKGKGVKLSITEERDRRRA 257
>SPAC959.07 |rps403|rps4-3, rps4|40S ribosomal protein
S4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 262
Score = 311 bits (763), Expect = 1e-85
Identities = 139/193 (72%), Positives = 161/193 (83%)
Frame = +1
Query: 64 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 243
M RGPKKHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1 MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60
Query: 244 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 423
V I+ QRLIKVDGKVRTD T+P GFMDV+S++KT E FRL+YD+KGRFT+HRIT EEAK
Sbjct: 61 VKAILMQRLIKVDGKVRTDSTFPTGFMDVISVDKTGEHFRLVYDIKGRFTVHRITAEEAK 120
Query: 424 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNL 603
YKLCKVKRV G K VP+LVTHDGRTIRYPDPLIKVND+I+L++ T KI FIKF++
Sbjct: 121 YKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQ 180
Query: 604 CMITGGRNLGACG 642
M+TGGRN+G G
Sbjct: 181 VMVTGGRNMGRVG 193
Score = 48.8 bits (111), Expect = 1e-06
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +2
Query: 632 GRVGTIVXRERHPGSFDIVHIKDSTGHTFATKV 730
GRVGTIV RE H GSF+I+H+KD+ FAT++
Sbjct: 190 GRVGTIVHREHHLGSFEIIHVKDALDREFATRL 222
Score = 47.6 bits (108), Expect = 2e-06
Identities = 20/36 (55%), Positives = 30/36 (83%)
Frame = +3
Query: 729 LNNVFIIGKGTXAYISLPRGKGIRLTIAEXRDKRIA 836
L+NVF+IG+ ++ISLP+GKG++L+I E RD+R A
Sbjct: 222 LSNVFVIGETGKSWISLPKGKGVKLSITEERDRRRA 257
>SPBC21B10.10 |rps402|rps4-2|40S ribosomal protein
S4|Schizosaccharomyces pombe|chr 2|||Manual
Length = 262
Score = 310 bits (762), Expect = 1e-85
Identities = 139/193 (72%), Positives = 161/193 (83%)
Frame = +1
Query: 64 MARGPKKHLKRLNAPKAWMLDKLGGVYAPRPSTGPHKLRECLPLVIFLRNRLKYALTGNE 243
M RGPKKHLKR+ AP W+LDKL G YAP+PS GPHK RECLPL++FLRNRLKYAL G E
Sbjct: 1 MVRGPKKHLKRVAAPHHWLLDKLSGTYAPKPSPGPHKARECLPLIVFLRNRLKYALNGRE 60
Query: 244 VLKIVKQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAK 423
V I+ QRLI+VDGKVRTD T+P GFMDV+S+EKT E FRL+YD+KGRFT+HRIT EEAK
Sbjct: 61 VKAILMQRLIQVDGKVRTDSTFPTGFMDVISVEKTGEHFRLVYDIKGRFTVHRITAEEAK 120
Query: 424 YKLCKVKRVATGPKNVPYLVTHDGRTIRYPDPLIKVNDSIQLDIATTKIMDFIKFESGNL 603
YKLCKVKRV G K VP+LVTHDGRTIRYPDPLIKVND+I+L++ T KI FIKF++
Sbjct: 121 YKLCKVKRVQLGAKGVPFLVTHDGRTIRYPDPLIKVNDTIKLNLETNKIESFIKFDTSAQ 180
Query: 604 CMITGGRNLGACG 642
M+TGGRN+G G
Sbjct: 181 VMVTGGRNMGRVG 193
Score = 48.8 bits (111), Expect = 1e-06
Identities = 20/33 (60%), Positives = 26/33 (78%)
Frame = +2
Query: 632 GRVGTIVXRERHPGSFDIVHIKDSTGHTFATKV 730
GRVGTIV RE H GSF+I+H+KD+ FAT++
Sbjct: 190 GRVGTIVHREHHLGSFEIIHVKDALDREFATRL 222
Score = 48.4 bits (110), Expect = 1e-06
Identities = 20/36 (55%), Positives = 30/36 (83%)
Frame = +3
Query: 729 LNNVFIIGKGTXAYISLPRGKGIRLTIAEXRDKRIA 836
L+NVF+IG+ ++ISLP+GKG++L+I E RD+R A
Sbjct: 222 LSNVFVIGEAGKSWISLPKGKGVKLSITEERDRRRA 257
>SPCC1450.11c |cek1||serine/threonine protein kinase
Cek1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1338
Score = 27.5 bits (58), Expect = 2.7
Identities = 17/55 (30%), Positives = 30/55 (54%), Gaps = 3/55 (5%)
Frame = -1
Query: 318 SSRISRVSPNFPIN--LYEALFH-NFQDFVSGQSILQTIPQENHQGQALAQLVGT 163
SS+ + P+ P++ +E F NFQ S L IP++ G ++A+++GT
Sbjct: 27 SSKDENLQPSIPLSPVAFELDFSGNFQFISDNSSELLDIPKDKIIGHSVAEVLGT 81
>SPBC18H10.10c |cwc16||complexed with Cdc5 protein
Cwf16|Schizosaccharomyces pombe|chr 2|||Manual
Length = 294
Score = 27.5 bits (58), Expect = 2.7
Identities = 20/81 (24%), Positives = 33/81 (40%), Gaps = 1/81 (1%)
Frame = +1
Query: 259 KQRLIKVDGKVRTDPTYPAGFMDVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCK 438
K++ K+ +R + +P + +I + F + G + +I K LC
Sbjct: 13 KRKFDKLRNVIRFEMPFPVWCNNCENIIQQGTRFNAVKKEIGSYYTTKIWSFSLKCHLCS 72
Query: 439 VK-RVATGPKNVPYLVTHDGR 498
V T PKN Y+V GR
Sbjct: 73 NPIDVHTDPKNTEYIVASGGR 93
>SPAC22F3.04 |mug62||AMP binding enzyme |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1428
Score = 26.6 bits (56), Expect = 4.7
Identities = 8/15 (53%), Positives = 12/15 (80%)
Frame = +3
Query: 525 QSQRFHPVRHRNYED 569
++++ HPVRH YED
Sbjct: 5 ENEKIHPVRHSKYED 19
>SPAC1F5.11c |||phosphatidylinositol kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 3655
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/62 (24%), Positives = 32/62 (51%), Gaps = 1/62 (1%)
Frame = +1
Query: 325 DVVSIEKTNELFRLIYDVKGRFTIHRITPEEAKYKLCKVKRVATGPKNVPYL-VTHDGRT 501
+V S+EK N++F + Y+V + + + K+ ++ ++ KNVP + V H
Sbjct: 1718 NVSSLEKCNQIFSIFYEVFFQHPSTNVYANDEGIKIGALQIISFFLKNVPEITVQHQTEM 1777
Query: 502 IR 507
++
Sbjct: 1778 LK 1779
>SPAC13G7.10 |mug152||transcription factor |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 390
Score = 26.2 bits (55), Expect = 6.2
Identities = 9/15 (60%), Positives = 10/15 (66%)
Frame = +3
Query: 492 WPHHPLPRPTYQSQR 536
WPHH LP + SQR
Sbjct: 265 WPHHNLPSQFFTSQR 279
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,494,948
Number of Sequences: 5004
Number of extensions: 74920
Number of successful extensions: 209
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 196
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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