Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_H21
(872 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 155 8e-39
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 83 7e-17
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 76 8e-15
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 73 4e-14
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 43 7e-05
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 42 9e-05
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 38 0.002
SPCC1020.12c ||SPCC14G10.06|xap-5-like protein|Schizosaccharomyc... 27 2.6
SPAC1296.04 |mug65||spore wall assembly protein |Schizosaccharom... 27 4.6
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.1
SPBC336.06c |rnh1||ribonuclease H Rnh1|Schizosaccharomyces pombe... 26 6.1
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 155 bits (376), Expect = 8e-39
Identities = 67/74 (90%), Positives = 70/74 (94%)
Frame = +1
Query: 244 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 423
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 424 VLLTEAPLNPKGQQ 465
LLTEAPLNPK +
Sbjct: 103 CLLTEAPLNPKSNR 116
Score = 124 bits (298), Expect = 2e-29
Identities = 92/239 (38%), Positives = 111/239 (46%), Gaps = 7/239 (2%)
Frame = +3
Query: 120 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG-------RDGRYGPERLLR 278
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G +D G E +
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 279 G*RGPEQERYPHSEVPHRARYHHQLG*HGEDLAPHLLQXXXXXXXXXXXXXXXXXXQPQG 458
RG +YP + H + E + H
Sbjct: 62 --RGILTLKYP---IEHGIVNNWD---DMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPK 113
Query: 459 PTGEKMTQIMFEXFNSPXMYVAIQAVLSLYASGSYHRVSCSTPEMVSPTPYPSTKVTLCP 638
EKMTQI+FE FN+P YVAIQAVLSLYASG + + + V+ T P + P
Sbjct: 114 SNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT-VPIYEGYALP 172
Query: 639 TPSSVWTWLVAXXXXXXXXXXXXXXXXXXXXLXXXIVRDIKXKLCYVALDFXQXMATXA 815
IVRDIK KLCYVALDF Q + T A
Sbjct: 173 HAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQELQTAA 231
Score = 75.4 bits (177), Expect = 1e-14
Identities = 36/55 (65%), Positives = 39/55 (70%)
Frame = +2
Query: 569 GIVLDSGDGVSHTVPIYEGYALPHAIXXXXXXXXXXXXXXMKILTXRGYSFTTTA 733
GIVLDSGDGV+HTVPIYEGYALPHAI MKIL RGY+F+TTA
Sbjct: 150 GIVLDSGDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTA 204
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 82.6 bits (195), Expect = 7e-17
Identities = 38/69 (55%), Positives = 49/69 (71%), Gaps = 1/69 (1%)
Frame = +1
Query: 244 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEH 420
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 421 PVLLTEAPL 447
P+LLTE PL
Sbjct: 107 PLLLTEPPL 115
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +3
Query: 126 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 239
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
Score = 43.6 bits (98), Expect = 4e-05
Identities = 16/26 (61%), Positives = 24/26 (92%)
Frame = +2
Query: 569 GIVLDSGDGVSHTVPIYEGYALPHAI 646
GIVL+ GDG++H+VPIY+G+++P AI
Sbjct: 155 GIVLECGDGLTHSVPIYDGFSIPSAI 180
Score = 29.9 bits (64), Expect = 0.49
Identities = 29/112 (25%), Positives = 42/112 (37%), Gaps = 4/112 (3%)
Frame = +3
Query: 468 EKMTQIMFEXFNSPXMYVAIQAVLSLYASGSYHRV--SCSTPEMVSPTPYPSTKVTLCPT 641
EK+ + +E N P + ++Q VL+LYAS + C S Y +
Sbjct: 122 EKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSI----- 176
Query: 642 PSSVWTWLVAXXXXXXXXXXXXXXXXXXXX--LXXXIVRDIKXKLCYVALDF 791
PS++ + IVR+IK K CYVA DF
Sbjct: 177 PSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVASDF 228
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 75.8 bits (178), Expect = 8e-15
Identities = 29/63 (46%), Positives = 48/63 (76%)
Frame = +1
Query: 265 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 444
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 445 LNP 453
+NP
Sbjct: 110 MNP 112
Score = 49.2 bits (112), Expect = 8e-07
Identities = 21/55 (38%), Positives = 30/55 (54%)
Frame = +2
Query: 569 GIVLDSGDGVSHTVPIYEGYALPHAIXXXXXXXXXXXXXXMKILTXRGYSFTTTA 733
G+V+DSGDGV+H VP+YE L H + + +L +GY+F TA
Sbjct: 150 GVVVDSGDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTA 204
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +3
Query: 132 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 230
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
Score = 40.7 bits (91), Expect = 3e-04
Identities = 21/38 (55%), Positives = 24/38 (63%), Gaps = 2/38 (5%)
Frame = +3
Query: 450 PQGPTG--EKMTQIMFEXFNSPXMYVAIQAVLSLYASG 557
P P EKM + MFE + +YVAIQAVLSLYA G
Sbjct: 109 PMNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQG 146
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 73.3 bits (172), Expect = 4e-14
Identities = 38/94 (40%), Positives = 53/94 (56%), Gaps = 4/94 (4%)
Frame = +1
Query: 184 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 351
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 352 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 453
WD ME+ W + + LR PE+H LLTE PLNP
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNP 135
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +3
Query: 138 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 221
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/36 (47%), Positives = 25/36 (69%)
Frame = +3
Query: 468 EKMTQIMFEXFNSPXMYVAIQAVLSLYASGSYHRVS 575
E +IMFE FN +Y+A+QAVL+L AS + +V+
Sbjct: 140 ENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVT 175
Score = 37.5 bits (83), Expect = 0.002
Identities = 14/26 (53%), Positives = 20/26 (76%)
Frame = +2
Query: 569 GIVLDSGDGVSHTVPIYEGYALPHAI 646
G V+DSGDGV+H +P+ EGY + +I
Sbjct: 181 GTVVDSGDGVTHIIPVAEGYVIGSSI 206
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 42.7 bits (96), Expect = 7e-05
Identities = 21/67 (31%), Positives = 39/67 (58%), Gaps = 1/67 (1%)
Frame = +1
Query: 256 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 432
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 433 TEAPLNP 453
TE NP
Sbjct: 121 TEPLANP 127
Score = 37.5 bits (83), Expect = 0.002
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +3
Query: 60 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 233
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 25.8 bits (54), Expect = 8.1
Identities = 9/19 (47%), Positives = 13/19 (68%)
Frame = +2
Query: 569 GIVLDSGDGVSHTVPIYEG 625
GIVL+ G+ SH +P+ G
Sbjct: 167 GIVLNLGNAASHVIPVLNG 185
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 42.3 bits (95), Expect = 9e-05
Identities = 17/48 (35%), Positives = 27/48 (56%)
Frame = +1
Query: 310 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNP 453
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNP 110
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +3
Query: 123 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 296
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 37.9 bits (84), Expect = 0.002
Identities = 20/66 (30%), Positives = 34/66 (51%), Gaps = 2/66 (3%)
Frame = +1
Query: 265 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 438
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 439 APLNPK 456
NP+
Sbjct: 109 PSWNPQ 114
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +3
Query: 123 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 224
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPCC1020.12c ||SPCC14G10.06|xap-5-like protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 288
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/19 (52%), Positives = 15/19 (78%)
Frame = +3
Query: 561 YHRVSCSTPEMVSPTPYPS 617
+ ++SCS+PEMV+ T PS
Sbjct: 219 FDKLSCSSPEMVATTQLPS 237
>SPAC1296.04 |mug65||spore wall assembly protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 248
Score = 26.6 bits (56), Expect = 4.6
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = +3
Query: 615 STKVTLCPTPSSVWTW 662
STK CP PS W W
Sbjct: 52 STKTATCPDPSWEWAW 67
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -3
Query: 348 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 235
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPBC336.06c |rnh1||ribonuclease H Rnh1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 264
Score = 26.2 bits (55), Expect = 6.1
Identities = 21/82 (25%), Positives = 32/82 (39%), Gaps = 7/82 (8%)
Frame = +3
Query: 21 HYREFLKILTRSLAAVESIPASPTQTHTKFKMCDDDVAALV----VDNGSGMCKAG---F 179
H ++ K ++RS + + I TH K C D + NG AG F
Sbjct: 88 HSDKYRKKISRSYSTEKDIEIFSNDTHEKSIACSDRQVVYADGSSLRNGKKGAVAGCGVF 147
Query: 180 AGDDAPRAVFPSIVGRPRHQGR 245
G+D PR + + G + R
Sbjct: 148 FGNDDPRNISVPLAGEEQTNNR 169
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,041,481
Number of Sequences: 5004
Number of extensions: 58315
Number of successful extensions: 211
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 170
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 209
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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