Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_H11
(945 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 35 0.019
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.18
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.72
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.3
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 5.1
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.7 bits (76), Expect = 0.019
Identities = 26/87 (29%), Positives = 32/87 (36%), Gaps = 1/87 (1%)
Frame = +2
Query: 167 PPPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXPGXTLXFS 346
PPP+ P P P SG PP+ + +PP P+ P P P P S
Sbjct: 1063 PPPVPAPSSEIPSIPAP-SGAPPVPA--PSGIPPVPKPSVAAPPVPKPSVAVPPVPAP-S 1118
Query: 347 PXPPLXSPQKXFLK-XXXXXAPPXPPP 424
PP+ P APP P P
Sbjct: 1119 GAPPVPKPSVAAPPVPVPSGAPPVPKP 1145
Score = 34.3 bits (75), Expect = 0.025
Identities = 25/85 (29%), Positives = 32/85 (37%)
Frame = +2
Query: 170 PPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXPGXTLXFSP 349
PP+ P P P +G PP+ + PP P+ G P P P T P +
Sbjct: 1169 PPVPAPSSGIPPVPKPAAGVPPV--PPPSEAPPVPKPSVGVPPVP-PPSTAPPVPTPSAG 1225
Query: 350 XPPLXSPQKXFLKXXXXXAPPXPPP 424
PP+ P APP P P
Sbjct: 1226 LPPVPVP--------TAKAPPVPAP 1242
Score = 33.9 bits (74), Expect = 0.034
Identities = 30/99 (30%), Positives = 33/99 (33%), Gaps = 1/99 (1%)
Frame = +2
Query: 170 PPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXPGXTLXFSP 349
PP+ P P P SG PP+ PP P G P P P P S
Sbjct: 1121 PPVPKPSVAAPPVPVP-SGAPPVPKPSVAA-PPVPA-PSGAPPVPKPSVAAPPVPAPSSG 1177
Query: 350 XPPLXSPQKXFLK-XXXXXAPPXPPPXXXGXFGXPPXXT 463
PP+ P APP P P G PP T
Sbjct: 1178 IPPVPKPAAGVPPVPPPSEAPPVPKP-SVGVPPVPPPST 1215
Score = 30.7 bits (66), Expect = 0.31
Identities = 29/111 (26%), Positives = 37/111 (33%), Gaps = 1/111 (0%)
Frame = +2
Query: 170 PPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXPGXTLXFSP 349
PP+ P P P PP+ + PP P+ P P P P +
Sbjct: 1092 PPVPKPSVAAPPVPKPSVAVPPVPA--PSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVA- 1148
Query: 350 XPPLXSPQ-KXFLKXXXXXAPPXPPPXXXGXFGXPPXXTKXFLXFXXXPPP 499
PP+ +P + APP P P G PP K PPP
Sbjct: 1149 APPVPAPSGAPPVPKPSVAAPPVPAPSS----GIPP-VPKPAAGVPPVPPP 1194
Score = 30.3 bits (65), Expect = 0.41
Identities = 17/58 (29%), Positives = 22/58 (37%)
Frame = +2
Query: 200 PXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXPGXTLXFSPXPPLXSPQ 373
P P P G PP+ + PP P G P P P P S P + +P+
Sbjct: 1198 PPVPKPSVGVPPVPPP--STAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPR 1253
Score = 29.9 bits (64), Expect = 0.55
Identities = 28/105 (26%), Positives = 31/105 (29%), Gaps = 5/105 (4%)
Frame = +2
Query: 200 PXXPXPXSGPPPLXTXXXTXLPPF--PQFXXGXXPXPXPXKTXPGXTLXFSPXPPLXSPQ 373
P P S PPPL P P+ P P T P L P P S +
Sbjct: 961 PRPAAPPSIPPPLPVSNILSSPTSEPPKDHPPSAPLSKPVSTSPAAPLARVPPVPKLSSK 1020
Query: 374 KXFLKXXXXXAPPXPPPXXXGXFGXP---PXXTKXFLXFXXXPPP 499
+ APP P P P P K PPP
Sbjct: 1021 APPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPP 1065
Score = 27.5 bits (58), Expect = 2.9
Identities = 25/97 (25%), Positives = 32/97 (32%), Gaps = 12/97 (12%)
Frame = +2
Query: 170 PPILXXIKXKPXXPXPXSGPPPL-------XTXXXTXLPPFPQFXXG----XXPXPXPXK 316
PP+ P P P + PP+ T PP P+ G P P P
Sbjct: 1012 PPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSS 1071
Query: 317 TXPGXTLXFSPXPPLXSPQK-XFLKXXXXXAPPXPPP 424
P S PP+ +P + APP P P
Sbjct: 1072 EIPSIPAP-SGAPPVPAPSGIPPVPKPSVAAPPVPKP 1107
Score = 27.1 bits (57), Expect = 3.8
Identities = 17/62 (27%), Positives = 20/62 (32%)
Frame = +2
Query: 170 PPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXPGXTLXFSP 349
PP+ P P P + PP LPP P P P P P + S
Sbjct: 1198 PPVPKPSVGVPPVPPPSTAPP--VPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTPRSS 1255
Query: 350 XP 355
P
Sbjct: 1256 VP 1257
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.5 bits (68), Expect = 0.18
Identities = 14/52 (26%), Positives = 17/52 (32%)
Frame = +2
Query: 170 PPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXGXXPXPXPXKTXP 325
PP+ P P PPP+ PP P P P P + P
Sbjct: 1690 PPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.72
Identities = 25/107 (23%), Positives = 30/107 (28%), Gaps = 1/107 (0%)
Frame = +2
Query: 137 PPKN*KXXXXPPPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXX-GXXPXPXPX 313
PP++ PP L + P P PP + LPP P P P
Sbjct: 365 PPRSAPSTGRQPPPLSSSRAVSNPPAP---PPAIPGRSAPALPPLGNASRTSTPPVPTPP 421
Query: 314 KTXPGXTLXFSPXPPLXSPQKXFLKXXXXXAPPXPPPXXXGXFGXPP 454
P P P P + P PP G PP
Sbjct: 422 SLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPP 468
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.7 bits (61), Expect = 1.3
Identities = 20/66 (30%), Positives = 23/66 (34%), Gaps = 3/66 (4%)
Frame = +2
Query: 167 PPPILXXIKXKPXXPXPXSGPPPLXTXXXTXLPPFPQFXXG---XXPXPXPXKTXPGXTL 337
PPP I P P P PPP PP P G P P P G +
Sbjct: 733 PPPPPAVIVPTPA-PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSR 791
Query: 338 XFSPXP 355
++P P
Sbjct: 792 YYAPAP 797
Score = 27.1 bits (57), Expect = 3.8
Identities = 16/50 (32%), Positives = 16/50 (32%), Gaps = 1/50 (2%)
Frame = +1
Query: 355 PPXXPPKXFFKXAXXXXAPXPPP-PXXGXFWXPPPXNXXXFXVXXXXPPP 501
PP PP P PPP P G PPP PPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 26.2 bits (55), Expect = 6.7
Identities = 16/54 (29%), Positives = 17/54 (31%)
Frame = +1
Query: 262 APXPPIXXGXXXXPXPXKNXPRXXPFXFXXXPPXXPPKXFFKXAXXXXAPXPPP 423
+P PP P P P P PP PP A P PPP
Sbjct: 731 SPPPPPPAVIVPTPAPAP-IPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 5.1
Identities = 19/71 (26%), Positives = 21/71 (29%)
Frame = +1
Query: 214 PXFXAPPFXNXXXNXFAPXPPIXXGXXXXPXPXKNXPRXXPFXFXXXPPXXPPKXFFKXA 393
P A P + + P PP N P P PP K A
Sbjct: 173 PPSAAAPATSLPSDYNPPPPPPPPPAVEDQAADANEPDDYYSSGRAVSPEIPPTYTPKQA 232
Query: 394 XXXXAPXPPPP 426
AP PPPP
Sbjct: 233 DPLPAPPPPPP 243
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,675,879
Number of Sequences: 5004
Number of extensions: 24757
Number of successful extensions: 68
Number of sequences better than 10.0: 5
Number of HSP's better than 10.0 without gapping: 23
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 44
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 481321826
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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