Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP13_F_H10
         (884 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase Gpd3|...   132   8e-32
SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase...   128   1e-30
SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|c...    27   4.7  

>SPBC354.12 |gpd3||glyceraldehyde 3-phosphate dehydrogenase
           Gpd3|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 335

 Score =  132 bits (318), Expect = 8e-32
 Identities = 70/117 (59%), Positives = 84/117 (71%), Gaps = 2/117 (1%)
 Frame = +3

Query: 321 LVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 500
           LV++G+ I V +ERDP  I W  +GAEYV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGHSIDVHNERDPANIKWSASGAEYVIESTGVFTTKETASAHLKGGAKRVIISAPSK 126

Query: 501 DAPMFVVGVNLXAYDP--LF*GHLKCFLHHKTVLPHLPKVIHDNFEIVEGLMTTVHA 665
           DAPMFVVGVNL  ++P      +  C  +    L  L KVI+D F I EGLMTTVHA
Sbjct: 127 DAPMFVVGVNLEKFNPSEKVISNASCTTN---CLAPLAKVINDTFGIEEGLMTTVHA 180



 Score = 98.3 bits (234), Expect = 1e-21
 Identities = 46/63 (73%), Positives = 53/63 (84%), Gaps = 1/63 (1%)
 Frame = +2

Query: 137 KIGINGFGRIGRLVLRASIEKGA-QVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 313
           K+GINGFGRIGR+VLR +I  G  QVVA+NDPFI LDYM Y+FKYDSTHGRF+GSVE + 
Sbjct: 5   KVGINGFGRIGRIVLRNAILTGKIQVVAVNDPFIDLDYMAYMFKYDSTHGRFEGSVETKG 64

Query: 314 GFL 322
           G L
Sbjct: 65  GKL 67



 Score = 89.4 bits (212), Expect = 6e-19
 Identities = 54/120 (45%), Positives = 65/120 (54%)
 Frame = +1

Query: 472 KKLLYQLPVLMPPCLLWVLTXKLMTPSFKVISNASCTTKLSCPTCQRLFMITXXXXXXXX 651
           K+++   P    P  +  +  +   PS KVISNASCTT    P   ++   T        
Sbjct: 117 KRVIISAPSKDAPMFVVGVNLEKFNPSEKVISNASCTTNCLAPLA-KVINDTFGIEEGLM 175

Query: 652 XXFMPTTATQKTVDGPSGKLWRDGRGAQQNIIPCPLXGCCQTCGLRLSPALNGKLTGMAF 831
                TTATQKTVDGPS K WR GRGA  NIIP    G  +  G ++ PALNGKLTGMAF
Sbjct: 176 TTVHATTATQKTVDGPSKKDWRGGRGASANIIPSS-TGAAKAVG-KVIPALNGKLTGMAF 233


>SPBC32F12.11 |tdh1|gpd1|glyceraldehyde-3-phosphate dehydrogenase
           Tdh1|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 336

 Score =  128 bits (309), Expect = 1e-30
 Identities = 68/117 (58%), Positives = 83/117 (70%), Gaps = 2/117 (1%)
 Frame = +3

Query: 321 LVVNGNKIAVFSERDPKAIPWGKAGAEYVVESTGVFTTTDKASAHLEGGAKKVIISAPSA 500
           LV++GN I V +ERDP  I W  +GA+YV+ESTGVFTT + ASAHL+GGAK+VIISAPS 
Sbjct: 67  LVIDGNAIDVHNERDPADIKWSTSGADYVIESTGVFTTQETASAHLKGGAKRVIISAPSK 126

Query: 501 DAPMFVVGVNLXAYDP--LF*GHLKCFLHHKTVLPHLPKVIHDNFEIVEGLMTTVHA 665
           DAPM+VVGVN   ++P      +  C  +    L  L KVI+D F I EGLMTTVHA
Sbjct: 127 DAPMYVVGVNEEKFNPSEKVISNASCTTN---CLAPLAKVINDTFGIEEGLMTTVHA 180



 Score =  101 bits (241), Expect = 2e-22
 Identities = 47/63 (74%), Positives = 54/63 (85%), Gaps = 1/63 (1%)
 Frame = +2

Query: 137 KIGINGFGRIGRLVLR-ASIEKGAQVVAINDPFIGLDYMVYLFKYDSTHGRFKGSVEVQD 313
           K+GINGFGRIGR+VLR A + K  QVVAINDPFI L+YM Y+FKYDSTHGRF GSVE++D
Sbjct: 5   KVGINGFGRIGRIVLRNALVAKTIQVVAINDPFIDLEYMAYMFKYDSTHGRFDGSVEIKD 64

Query: 314 GFL 322
           G L
Sbjct: 65  GKL 67



 Score = 89.0 bits (211), Expect = 8e-19
 Identities = 54/120 (45%), Positives = 65/120 (54%)
 Frame = +1

Query: 472 KKLLYQLPVLMPPCLLWVLTXKLMTPSFKVISNASCTTKLSCPTCQRLFMITXXXXXXXX 651
           K+++   P    P  +  +  +   PS KVISNASCTT    P   ++   T        
Sbjct: 117 KRVIISAPSKDAPMYVVGVNEEKFNPSEKVISNASCTTNCLAPLA-KVINDTFGIEEGLM 175

Query: 652 XXFMPTTATQKTVDGPSGKLWRDGRGAQQNIIPCPLXGCCQTCGLRLSPALNGKLTGMAF 831
                TTATQKTVDGPS K WR GRGA  NIIP    G  +  G ++ PALNGKLTGMAF
Sbjct: 176 TTVHATTATQKTVDGPSKKDWRGGRGASANIIPSS-TGAAKAVG-KVIPALNGKLTGMAF 233


>SPAC1952.09c |||acetyl-CoA hydrolase|Schizosaccharomyces pombe|chr
           1|||Manual
          Length = 521

 Score = 26.6 bits (56), Expect = 4.7
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +3

Query: 558 GHLKCFLHHKTVLPHLPKVIH 620
           GHL  F HH+  +  LPK +H
Sbjct: 251 GHLVDFFHHEVSVGRLPKNLH 271


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,703,452
Number of Sequences: 5004
Number of extensions: 79005
Number of successful extensions: 236
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 226
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)