Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_G23
(882 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 217 1e-57
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 120 2e-28
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 93 5e-20
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 62 1e-10
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 54 4e-08
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 50 3e-07
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 44 3e-05
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 31 0.22
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 30 0.50
SPAC20G8.05c |cdc15||cell division control protein Cdc15|Schizos... 28 1.5
SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual 27 3.5
SPAC637.07 |moe1||translation initiation factor eIF3d Moe1|Schiz... 27 4.7
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 4.7
SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyc... 27 4.7
SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces... 27 4.7
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 26 8.2
SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase Tor2|S... 26 8.2
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 217 bits (531), Expect = 1e-57
Identities = 122/228 (53%), Positives = 139/228 (60%), Gaps = 1/228 (0%)
Frame = +3
Query: 162 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSK 341
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEAQSK
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 342 RGILTLKYPIEHGIVTNWDDMGEDLASYLLQ*AACRPRGTPSPAH*GSPQPKANREKMTX 521
RGILTLKYPIEHGIV NWDDM + P P PK+NREKMT
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 522 IMFETFNTPR-HVRRHPSRALAVRVRSYHRYRAGLRRRCLPXRAPSTKDTHSPXPSCVWX 698
I+FETFN P +V +L R+ + P + P
Sbjct: 122 IIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDS--GDGVTHTVPIYEGYALPHAIMRLD 179
Query: 699 XPGRDLTDYLMXILTERGYSSXPPPXGKIVREIKEXLXXXXLEXXQEM 842
GRDLTDYLM IL ERGY+ +IVR+IKE L L+ QE+
Sbjct: 180 LAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVALDFEQEL 227
Score = 128 bits (309), Expect = 1e-30
Identities = 61/82 (74%), Positives = 65/82 (79%)
Frame = +1
Query: 406 EKIWHHTFYNELRVAPEEHPVLLTEAPLNPRPTERR*PXSCSKHSTRPAMYVAIQAVLSL 585
EKIWHHTFYNELRVAPEEHP LLTEAPLNP+ + + PA YVAIQAVLSL
Sbjct: 83 EKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSL 142
Query: 586 YASGRTTGIVLDSGDGVSXTVP 651
YASGRTTGIVLDSGDGV+ TVP
Sbjct: 143 YASGRTTGIVLDSGDGVTHTVP 164
Score = 33.1 bits (72), Expect = 0.054
Identities = 14/19 (73%), Positives = 15/19 (78%)
Frame = +2
Query: 650 PIYEGYALPXAILRXXLXG 706
PIYEGYALP AI+R L G
Sbjct: 164 PIYEGYALPHAIMRLDLAG 182
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 120 bits (290), Expect = 2e-28
Identities = 81/215 (37%), Positives = 106/215 (49%), Gaps = 7/215 (3%)
Frame = +3
Query: 180 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 359
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EAQ+ RG+L +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRGLLKI 71
Query: 360 KYPIEHGIVTNWDDMGEDLASYLLQ*AACRPRGTPSPAH*GSPQPKA---NREKMTXIMF 530
+ PIE GI+ NW DM E++ SY+ P P P A N+EK+ +
Sbjct: 72 QRPIERGIIQNWSDM-EEIWSYIYSDQQLNTLPEEHPLLLTEP-PLANIRNKEKIAEYFY 129
Query: 531 ETFNTPR-HVRRHPSRALAVRVRSYHRYRAGLRRRC---LPXRAPSTKDTHSPXPSCVWX 698
ET N P P AL R+ G+ C L P P
Sbjct: 130 ETLNVPALSFSLQPVLALYASART-----TGIVLECGDGLTHSVPIYDGFSIPSAIQQEE 184
Query: 699 XPGRDLTDYLMXILTERGYSSXPPPXGKIVREIKE 803
GRD+TDYL L + G+ +IVREIKE
Sbjct: 185 IGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKE 219
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 93.1 bits (221), Expect = 5e-20
Identities = 73/224 (32%), Positives = 105/224 (46%), Gaps = 10/224 (4%)
Frame = +3
Query: 168 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAQSK 341
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 342 RGILTLKYPIEHGIVTNWDDMGEDLASYLLQ*AACRPRGTPSPAH*GSPQPKANREKMTX 521
R +L +KYP+E+GI+ ++++M + + PRG P ANREKM
Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCE 121
Query: 522 IMFETFNTPRHVRRHPSRALAVRVRS-YHRYRAGLRRRCLPXRAPSTKDTHSPXPSCV-- 692
MFE R+ + V +++ Y GL + S V
Sbjct: 122 TMFE---------RYGFGGVYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLN 172
Query: 693 -----WXXPGRDLTDYLMXILTERGYSSXPPPXGKIVREIKEXL 809
GRD T YL+ +L +GY+ + VRE+KE L
Sbjct: 173 HLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKL 216
Score = 77.0 bits (181), Expect = 3e-15
Identities = 35/81 (43%), Positives = 53/81 (65%)
Frame = +1
Query: 409 KIWHHTFYNELRVAPEEHPVLLTEAPLNPRPTERR*PXSCSKHSTRPAMYVAIQAVLSLY 588
++W +TF+ +L++ P +LLTE P+NP + + + +YVAIQAVLSLY
Sbjct: 84 QLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETMFERYGFGGVYVAIQAVLSLY 143
Query: 589 ASGRTTGIVLDSGDGVSXTVP 651
A G ++G+V+DSGDGV+ VP
Sbjct: 144 AQGLSSGVVVDSGDGVTHIVP 164
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 62.1 bits (144), Expect = 1e-10
Identities = 46/145 (31%), Positives = 63/145 (43%), Gaps = 24/145 (16%)
Frame = +3
Query: 180 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 308
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 309 ------DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMGEDLASYLLQ*AACRPRGTPS 467
D ++G++A K +L YPI HG + NWD M L + C P
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 468 PAH*GSPQPKANREKMTXIMFETFN 542
P NRE IMFE+FN
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFN 151
Score = 61.7 bits (143), Expect = 1e-10
Identities = 38/91 (41%), Positives = 50/91 (54%), Gaps = 9/91 (9%)
Frame = +1
Query: 406 EKIWHHTFYNELRVAPEEHPVLLTEAPLNPRPTERR*PXSCSKHSTRPA-MYVAIQAVLS 582
E+ W + + LR PE+H LLTE PLNP P R S A +Y+A+QAVL+
Sbjct: 106 ERFWQQSLFKYLRCEPEDHYFLLTEPPLNP-PENRENTAEIMFESFNCAGLYIAVQAVLA 164
Query: 583 LYASGRT--------TGIVLDSGDGVSXTVP 651
L AS + TG V+DSGDGV+ +P
Sbjct: 165 LAASWTSSKVTDRSLTGTVVDSGDGVTHIIP 195
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 53.6 bits (123), Expect = 4e-08
Identities = 35/124 (28%), Positives = 59/124 (47%), Gaps = 1/124 (0%)
Frame = +3
Query: 180 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTL 359
LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++ + G ++
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDR----KLSRTSTLVGNDTLIEVGSRSI 82
Query: 360 -KYPIEHGIVTNWDDMGEDLASYLLQ*AACRPRGTPSPAH*GSPQPKANREKMTXIMFET 536
+ P E +++NWD M E + Y P P R MT ++FE
Sbjct: 83 ARSPFERNVISNWDLM-EQVLDYTFLKLGIDRMEHPICMTEPLANPTYVRSTMTELLFEL 141
Query: 537 FNTP 548
+N P
Sbjct: 142 YNAP 145
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 50.4 bits (115), Expect = 3e-07
Identities = 42/129 (32%), Positives = 59/129 (45%), Gaps = 1/129 (0%)
Frame = +3
Query: 165 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 344
+EV+A+V+D GS + GF+G+D P+ V PS G + G + Y+ +S
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGEFSDGRRLFG----EEYI---YKSNP 61
Query: 345 GILTLKYPIEHGIVTNWDDMGEDLASY-LLQ*AACRPRGTPSPAH*GSPQPKANREKMTX 521
G + +K I +G V NW D+ DL Y L Q P P P NR K
Sbjct: 62 G-MEIKNAIRNGWVENW-DVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLE 119
Query: 522 IMFETFNTP 548
MFE+ P
Sbjct: 120 TMFESLRCP 128
Score = 45.2 bits (102), Expect = 1e-05
Identities = 26/73 (35%), Positives = 38/73 (52%), Gaps = 1/73 (1%)
Frame = +1
Query: 412 IWHHTFYNELRVAPEEHPVLLTEAPLNPRPTERR*PXSCSKHSTR-PAMYVAIQAVLSLY 588
+W + +L+ P EHP+L+TE NP P R S R PA Y+A Q + +
Sbjct: 83 LWRYGLEQQLKTNPLEHPILITEPFDNP-PENRVKTLETMFESLRCPATYLAKQETCAAF 141
Query: 589 ASGRTTGIVLDSG 627
ASG+ T ++D G
Sbjct: 142 ASGKGTACLVDIG 154
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 44.0 bits (99), Expect = 3e-05
Identities = 22/78 (28%), Positives = 41/78 (52%), Gaps = 1/78 (1%)
Frame = +1
Query: 415 WHHTFYNELRVAPEEHPVLLTEAPLNPRPTERR*PXSCSKHSTRPAMYVAIQAVLSLYAS 594
W +L+V P E+ +++TE NP+ ++ + + PA Y+ QAV +A+
Sbjct: 87 WERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFAN 146
Query: 595 GRTTGIVLDSG-DGVSXT 645
++T +++D G D S T
Sbjct: 147 SKSTALIVDIGSDNASVT 164
Score = 43.6 bits (98), Expect = 4e-05
Identities = 25/80 (31%), Positives = 44/80 (55%), Gaps = 2/80 (2%)
Frame = +3
Query: 165 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKR 344
EE+ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 345 GI--LTLKYPIEHGIVTNWD 398
I + +K +GI+ +W+
Sbjct: 62 PIPGMEVKNGKSNGIIQDWE 81
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 31.1 bits (67), Expect = 0.22
Identities = 16/48 (33%), Positives = 26/48 (54%)
Frame = +3
Query: 225 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAQSKRGILTLKYP 368
G+ PRA F ++ P H G+++ M KD G+E S +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 29.9 bits (64), Expect = 0.50
Identities = 19/55 (34%), Positives = 23/55 (41%), Gaps = 1/55 (1%)
Frame = -3
Query: 322 PT*ESFCPI-PTITP*WRGLPTIEGNTARGASSPAKPALHIPEPLSTTNAATSSS 161
PT S P+ PT+ P P N S P PL+TTN TS+S
Sbjct: 401 PTGNSTTPVTPTVPPTSSSTPLTTTNCTTSTSVPYTSTPVTSTPLATTNCTTSTS 455
>SPAC20G8.05c |cdc15||cell division control protein
Cdc15|Schizosaccharomyces pombe|chr 1|||Manual
Length = 927
Score = 28.3 bits (60), Expect = 1.5
Identities = 13/30 (43%), Positives = 16/30 (53%)
Frame = -1
Query: 333 VPHLLHKSPSVPYRPSRPDGGAFPRSRGTR 244
+ L+ K+PS PY SRP A S TR
Sbjct: 306 IDQLISKAPSYPYSSSRPSASASLASSPTR 335
>SPCC622.02 |||dubious|Schizosaccharomyces pombe|chr 3|||Manual
Length = 127
Score = 27.1 bits (57), Expect = 3.5
Identities = 23/60 (38%), Positives = 28/60 (46%)
Frame = -2
Query: 362 FEGQDTSFALCLISYIRVLLSHTDHHALMAGPSHDRGEHGARSIISCETGLAHTGAIVYY 183
F G +F CLI++IR +SH D EHG II C T L G I+YY
Sbjct: 47 FAGLGIAFIYCLIAFIRE-MSHPSSR-------KDTMEHGL-PIILCST-LMLVGNILYY 96
>SPAC637.07 |moe1||translation initiation factor eIF3d
Moe1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 567
Score = 26.6 bits (56), Expect = 4.7
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = -1
Query: 675 GSAYPS*MGHGXGDTVAGVQHDTGGTTGR 589
G Y S G G GDTV GG GR
Sbjct: 131 GGRYGSSGGRGAGDTVVSRSSGAGGARGR 159
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.6 bits (56), Expect = 4.7
Identities = 17/41 (41%), Positives = 21/41 (51%), Gaps = 2/41 (4%)
Frame = -3
Query: 325 SPT*ESFC--PIPTITP*WRGLPTIEGNTARGASSPAKPAL 209
S T +SF P+PT P LPT NT + P+ PAL
Sbjct: 64 SNTPKSFAAPPVPTGAP---SLPTSSNNTQQAEERPSMPAL 101
>SPBC12C2.06 |||ATP-dependent RNA helicase Dbp5|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 503
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/33 (33%), Positives = 16/33 (48%)
Frame = +3
Query: 654 STKDTHSPXPSCVWXXPGRDLTDYLMXILTERG 752
S D P P + P R+L +M ++TE G
Sbjct: 176 SRVDASVPKPQAICLAPSRELARQIMDVVTEMG 208
>SPBC21D10.12 |hob1||BAR adaptor protein Hob1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 466
Score = 26.6 bits (56), Expect = 4.7
Identities = 12/35 (34%), Positives = 14/35 (40%)
Frame = +2
Query: 548 PPCTSPSKPCSRCTRPVVPPVSCWTPATVSPXPCP 652
PP P + T PV PV+ A P P P
Sbjct: 367 PPPVMPQPAAAAVTTPVAAPVAAAAAAVPVPPPAP 401
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/44 (29%), Positives = 26/44 (59%)
Frame = -2
Query: 410 FSHVIPVSDDSVFDGVFEGQDTSFALCLISYIRVLLSHTDHHAL 279
F+H+IPV D SV + + D L L +Y++ ++S+ ++ +
Sbjct: 167 FTHIIPVIDFSVQEQAVKRVDVGGKL-LTNYLKEVISYRKYNMM 209
>SPBC216.07c |tor2|SPBC646.01c|phosphatidylinositol kinase
Tor2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2337
Score = 25.8 bits (54), Expect = 8.2
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = -2
Query: 362 FEGQDTSFALCLISYIRVLLSHTDHHA 282
+EG+DT+ +Y+R++L TD A
Sbjct: 74 YEGEDTTRITRFANYLRIILPGTDQKA 100
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,385,570
Number of Sequences: 5004
Number of extensions: 72904
Number of successful extensions: 214
Number of sequences better than 10.0: 17
Number of HSP's better than 10.0 without gapping: 174
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 198
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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