BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_G07
(1412 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.17
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.39
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 31 0.39
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 31 0.39
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 30 0.89
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 28 2.7
SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr 1|||M... 27 4.7
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 27 8.3
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 27 8.3
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.3 bits (70), Expect = 0.17
Identities = 32/125 (25%), Positives = 37/125 (29%), Gaps = 1/125 (0%)
Frame = +2
Query: 707 PIISXXSXXPXPXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXDSXXXXXS 886
P I+ S P P PSSR + PP P P S PP+ S
Sbjct: 282 PAINSTSKPPLPPPSSR--VSAAALAANKKRPPPPPPPSR--RNRGKPPIGNGSSNSSLP 337
Query: 887 XRPPXHXXXXLXXXXXXPQXLSRPPXXTXPXRXVXTXXPNXXRXSXIXXXPXRPSPSL-X 1063
PP PQ S PP P S P P P++
Sbjct: 338 PPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPPAPPPAIPG 397
Query: 1064 XXAPA 1078
APA
Sbjct: 398 RSAPA 402
Score = 30.3 bits (65), Expect = 0.67
Identities = 18/54 (33%), Positives = 22/54 (40%)
Frame = +2
Query: 707 PIISXXSXXPXPXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXDS 868
P +S + P P PS+ PP P P P P S T PP+P S
Sbjct: 246 PSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINS---TSKPPLPPPS 296
Score = 29.9 bits (64), Expect = 0.89
Identities = 18/56 (32%), Positives = 21/56 (37%), Gaps = 2/56 (3%)
Frame = +2
Query: 698 PXRPIISXXSXXPXPXPSSRXXXP--PPRXPLXPRXPPLPXPXSXSPXXTXSPPVP 859
P P + S P PS P PP P P PPLP +P + P P
Sbjct: 421 PSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAP 476
Score = 27.9 bits (59), Expect = 3.6
Identities = 30/117 (25%), Positives = 36/117 (30%), Gaps = 7/117 (5%)
Frame = +2
Query: 725 SXXPXPXPSSRXXXPPPRXP--LXPRXPPLPXPXSXS-PXXTXSPPVPXD----SXXXXX 883
S P P PS PP R P P PP+P P + + SPP P +
Sbjct: 228 SAPPIP-PSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINS 286
Query: 884 SXRPPXHXXXXLXXXXXXPQXLSRPPXXTXPXRXVXTXXPNXXRXSXIXXXPXRPSP 1054
+ +PP RPP P R P S P P P
Sbjct: 287 TSKPPLPPPSSRVSAAALAANKKRPPPPPPPSRRNRGKPPIGNGSSNSSLPPPPPPP 343
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.1 bits (67), Expect = 0.39
Identities = 15/44 (34%), Positives = 15/44 (34%)
Frame = +2
Query: 734 PXPXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXD 865
P P P PPP P P PP P S P P D
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGD 1748
Score = 29.5 bits (63), Expect = 1.2
Identities = 18/53 (33%), Positives = 19/53 (35%)
Frame = +2
Query: 740 PXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXDSXXXXXSXRPP 898
P PSS P P L P P +P P S P PP S PP
Sbjct: 1470 PAPSSAPAPPAPVSQLPPAVPNVPVP-SMIPSVAQQPPSSVAPATAPSSTLPP 1521
Score = 29.1 bits (62), Expect = 1.6
Identities = 17/49 (34%), Positives = 21/49 (42%), Gaps = 1/49 (2%)
Frame = +3
Query: 708 LSSAVPRXXPXXXPXXAXXPPPPXXXSPPXXPLSXXPXPX-PPXXXPPP 851
++ A P P P A PP +PP P+S P P PP PP
Sbjct: 1681 MAPAHPVSTPPVRPQSAA-PPQMSAPTPPPPPMSVPPPPSAPPMPAGPP 1728
Score = 29.1 bits (62), Expect = 1.6
Identities = 14/57 (24%), Positives = 18/57 (31%)
Frame = +2
Query: 698 PXRPIISXXSXXPXPXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXDS 868
P P+ + P PP P+ PP P P PP+P S
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739
Score = 28.7 bits (61), Expect = 2.1
Identities = 21/74 (28%), Positives = 23/74 (31%), Gaps = 2/74 (2%)
Frame = +3
Query: 747 PXXAXXPPPPXXXSPPXXPLSXXPXPXPPXXXPPPXSXXTXXXXPXLAXPXXT--AXXXS 920
P A PP P PP P P P PP S P + A S
Sbjct: 1472 PSSAPAPPAPVSQLPPAVPNVPVPSMIPSVAQQPPSSVAPATAPSSTLPPSQSSFAHVPS 1531
Query: 921 PXXXQXRXPSPARL 962
P + PS A L
Sbjct: 1532 PAPPAPQHPSAAAL 1545
Score = 28.3 bits (60), Expect = 2.7
Identities = 16/59 (27%), Positives = 18/59 (30%)
Frame = +3
Query: 747 PXXAXXPPPPXXXSPPXXPLSXXPXPXPPXXXPPPXSXXTXXXXPXLAXPXXTAXXXSP 923
P PP S +S P PP PPP S P A P +P
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 31.1 bits (67), Expect = 0.39
Identities = 18/72 (25%), Positives = 23/72 (31%)
Frame = +2
Query: 746 PSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXDSXXXXXSXRPPXHXXXXLXX 925
P S P P+ L P P P P +PP+P + S PP +
Sbjct: 124 PPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKS 183
Query: 926 XXXXPQXLSRPP 961
P S P
Sbjct: 184 PPSAPSLPSAVP 195
Score = 28.7 bits (61), Expect = 2.1
Identities = 22/78 (28%), Positives = 24/78 (30%), Gaps = 7/78 (8%)
Frame = +3
Query: 711 SSAVPRXXPXXXPXXAXXPP---PPXXXSPPXXPLSXXPXPXP----PXXXPPPXSXXTX 869
S+A P P PP PP PP P S P P P PPP
Sbjct: 121 SAAPPSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAP 180
Query: 870 XXXPXLAXPXXTAXXXSP 923
P A +A P
Sbjct: 181 VKSPPSAPSLPSAVPPMP 198
Score = 27.9 bits (59), Expect = 3.6
Identities = 18/57 (31%), Positives = 21/57 (36%), Gaps = 5/57 (8%)
Frame = +3
Query: 702 PXLSSAVPRXXPXXXPXXAXXPPPPXXX----SPPXXP-LSXXPXPXPPXXXPPPXS 857
P ++ P P + PPP SPP P L P PP PPP S
Sbjct: 151 PPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPPLS 207
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.1 bits (67), Expect = 0.39
Identities = 17/50 (34%), Positives = 19/50 (38%)
Frame = +3
Query: 702 PXLSSAVPRXXPXXXPXXAXXPPPPXXXSPPXXPLSXXPXPXPPXXXPPP 851
P SS +P P P P PP +PP S P PP PP
Sbjct: 1172 PAPSSGIP---PVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPP 1218
Score = 30.3 bits (65), Expect = 0.67
Identities = 18/57 (31%), Positives = 23/57 (40%), Gaps = 3/57 (5%)
Frame = +2
Query: 707 PIISXXSXXPXPXPSSRXXXPPPRXPLXPRXPPLPXPXS---XSPXXTXSPPVPXDS 868
PI + P P P+S P PP+P P S P + +PPVP S
Sbjct: 1033 PIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPS 1089
Score = 29.5 bits (63), Expect = 1.2
Identities = 17/59 (28%), Positives = 19/59 (32%)
Frame = +3
Query: 720 VPRXXPXXXPXXAXXPPPPXXXSPPXXPLSXXPXPXPPXXXPPPXSXXTXXXXPXLAXP 896
+P P + PP P S P P S P P P P S P A P
Sbjct: 1026 LPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPP 1084
Score = 29.1 bits (62), Expect = 1.6
Identities = 24/103 (23%), Positives = 28/103 (27%)
Frame = +3
Query: 645 PXYXPXXXXPXXXXXXXXXPXLSSAVPRXXPXXXPXXAXXPPPPXXXSPPXXPLSXXPXP 824
P P P P + P P A P P PP P S P
Sbjct: 1160 PVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPV 1219
Query: 825 XPPXXXPPPXSXXTXXXXPXLAXPXXTAXXXSPXXXQXRXPSP 953
P PP T P P ++ S + PSP
Sbjct: 1220 PTPSAGLPPVPVPTAKAPP---VPAPSSEAPSVSTPRSSVPSP 1259
Score = 27.9 bits (59), Expect = 3.6
Identities = 18/48 (37%), Positives = 19/48 (39%), Gaps = 3/48 (6%)
Frame = +2
Query: 734 PXPXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXS---PPVPXDS 868
P P PSS P P PP+P P P S PPVP S
Sbjct: 1065 PVPAPSSEI----PSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPS 1108
Score = 27.5 bits (58), Expect = 4.7
Identities = 13/41 (31%), Positives = 21/41 (51%), Gaps = 3/41 (7%)
Frame = +2
Query: 746 PSSRXXXPPPRXPLXPRXPPLPXPXSXS---PXXTXSPPVP 859
P++ PP L + PP+P P + + P + +PPVP
Sbjct: 1004 PAAPLARVPPVPKLSSKAPPVPLPSADAPPIPVPSTAPPVP 1044
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 29.9 bits (64), Expect = 0.89
Identities = 17/40 (42%), Positives = 17/40 (42%)
Frame = -3
Query: 858 GTGGEXVSXGDXEXGXGRGGXRGXXGXRGGGXXXRXEGXG 739
G GG G G GRGG RG G RGG R G
Sbjct: 24 GRGGFGGGRGGARGG-GRGGARGGRGGRGGARGGRGGSSG 62
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 28.3 bits (60), Expect = 2.7
Identities = 13/44 (29%), Positives = 20/44 (45%), Gaps = 2/44 (4%)
Frame = +2
Query: 704 RPIISXXSXXPXPXPSSRXXXPPP--RXPLXPRXPPLPXPXSXS 829
+P ++ P P P+ + PPP R + P PP P + S
Sbjct: 724 KPQVTPAPPTPAPTPAVKHHPPPPPVRSSISPSMPPAPLTHANS 767
>SPAC140.02 |gar2||GAR family|Schizosaccharomyces pombe|chr
1|||Manual
Length = 500
Score = 27.5 bits (58), Expect = 4.7
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = -1
Query: 851 GGRXXXGGXGXRXXGEGGXGGXXXXGGGG 765
GGR GG G G GG GG G GG
Sbjct: 451 GGRGGFGGRGG-FGGRGGFGGGRGRGRGG 478
Score = 26.6 bits (56), Expect = 8.3
Identities = 15/38 (39%), Positives = 15/38 (39%)
Frame = -2
Query: 847 GGXXXGGXGXGXXERGXXGGEXXXGGGGXXAXXGXXXG 734
GG GG G G RG GG GGG G G
Sbjct: 446 GGGSRGGRG-GFGGRGGFGGRGGFGGGRGRGRGGARSG 482
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 26.6 bits (56), Expect = 8.3
Identities = 14/44 (31%), Positives = 16/44 (36%)
Frame = +2
Query: 734 PXPXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVPXD 865
P P P P PL P PPLP PP+P +
Sbjct: 102 PLPREPPLPNEPVPEEPL-PGEPPLPDEPVPEEPLPGEPPLPNE 144
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 26.6 bits (56), Expect = 8.3
Identities = 12/40 (30%), Positives = 15/40 (37%)
Frame = +2
Query: 740 PXPSSRXXXPPPRXPLXPRXPPLPXPXSXSPXXTXSPPVP 859
P P+ PP P+ PP P P + PP P
Sbjct: 742 PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,482,116
Number of Sequences: 5004
Number of extensions: 13785
Number of successful extensions: 206
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 35
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 784935310
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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