Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_E17
(894 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein L3|Sch... 288 8e-79
SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3 |Schiz... 287 1e-78
SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 26 0.97
SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces p... 27 4.8
SPBC1683.12 |||nicotinic acid plasma membrane transporter |Schiz... 26 6.3
SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyce... 26 8.3
>SPAPB8E5.06c |rpl302|rpl3-2, rpl3-b|60S ribosomal protein
L3|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 288 bits (706), Expect = 8e-79
Identities = 146/274 (53%), Positives = 189/274 (68%), Gaps = 2/274 (0%)
Frame = +3
Query: 72 HRKFSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKAGMTHVVREPDR 251
H KF PRHGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+GYKAGMTH+VR+ DR
Sbjct: 3 HCKFEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLGYKAGMTHIVRDLDR 62
Query: 252 PGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRFYKNW 431
PGSK++K+EI+EAVTIIETPPMV VGVVGY+ETP GLR+L TVWAEH+SE+ +RRFYKNW
Sbjct: 63 PGSKMHKREILEAVTIIETPPMVVVGVVGYVETPRGLRSLTTVWAEHLSEEVKRRFYKNW 122
Query: 432 YXXXXXXXXXXXXXWQDELGRKSIEKDFKKMIRYCSVVRVIAHTQMKLLKQRQKKAHIME 611
+ + + +SI ++ +++ +YCSVVRV+AHTQ++ QKKAH+ME
Sbjct: 123 FKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQIRKTPLAQKKAHLME 180
Query: 612 IQLNGGTIEDKVKWAREHLEKPIPGRFCVCPR*ND*LHWCHQGQRIQRCHFSLAHKRSYP 791
IQ+NGG++ DKV+WAREH EK + + + +G+ + KR P
Sbjct: 181 IQVNGGSVADKVEWAREHFEKTVDIKSTFEQNEMIDVIGVTRGKGNEGTTARWGTKR-LP 239
Query: 792 GKTHKGLXK-SCL-HWSLASF*GVVXVXRAGQKG 887
KTH+GL K +C+ W A+ V RAG G
Sbjct: 240 RKTHRGLRKVACIGAWHPANV--QWTVARAGNAG 271
Score = 48.8 bits (111), Expect = 1e-06
Identities = 25/55 (45%), Positives = 29/55 (52%)
Frame = +1
Query: 685 VDSVFAQDEMIDCIXXXXXXXXXXXXSRWHTKEATPVRHTRVLXKVACIGAWHPS 849
+ S F Q+EMID I +RW TK H R L KVACIGAWHP+
Sbjct: 205 IKSTFEQNEMIDVIGVTRGKGNEGTTARWGTKRLPRKTH-RGLRKVACIGAWHPA 258
>SPAC17A5.03 |rpl301|rpl3-1, rpl3|60S ribosomal protein L3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 388
Score = 287 bits (705), Expect = 1e-78
Identities = 145/274 (52%), Positives = 189/274 (68%), Gaps = 2/274 (0%)
Frame = +3
Query: 72 HRKFSAPRHGSMGFYPKKRSRRHRGKVKAFPKDDPSKPVHLTAFIGYKAGMTHVVREPDR 251
H KF PRHGS+GF P+KR+ R RGKVKAFPKDD SKPVHLTAF+GYKAGMTH+VR+ DR
Sbjct: 3 HCKFEQPRHGSLGFLPRKRASRQRGKVKAFPKDDASKPVHLTAFLGYKAGMTHIVRDLDR 62
Query: 252 PGSKINKKEIVEAVTIIETPPMVCVGVVGYIETPHGLRALLTVWAEHMSEDCRRRFYKNW 431
PGSK++K+EI+EAVT+IETPPMV VGVVGY+ETP GLR+L TVWAEH+SE+ +RRFYKNW
Sbjct: 63 PGSKMHKREILEAVTVIETPPMVVVGVVGYVETPRGLRSLTTVWAEHLSEEVKRRFYKNW 122
Query: 432 YXXXXXXXXXXXXXWQDELGRKSIEKDFKKMIRYCSVVRVIAHTQMKLLKQRQKKAHIME 611
+ + + +SI ++ +++ +YCSVVRV+AHTQ++ QKKAH+ME
Sbjct: 123 FKSKKKAFTKYAKKYAE--STQSINRELERIKKYCSVVRVLAHTQIRKTPLAQKKAHLME 180
Query: 612 IQLNGGTIEDKVKWAREHLEKPIPGRFCVCPR*ND*LHWCHQGQRIQRCHFSLAHKRSYP 791
IQ+NGG++ DKV+WAREH EK + + + +G+ + KR P
Sbjct: 181 IQVNGGSVADKVEWAREHFEKTVDIKSTFEQNEMIDVIGVTRGKGNEGTTARWGTKR-LP 239
Query: 792 GKTHKGLXK-SCL-HWSLASF*GVVXVXRAGQKG 887
KTH+GL K +C+ W A+ V RAG G
Sbjct: 240 RKTHRGLRKVACIGAWHPANV--QWTVARAGNAG 271
Score = 48.8 bits (111), Expect = 1e-06
Identities = 25/55 (45%), Positives = 29/55 (52%)
Frame = +1
Query: 685 VDSVFAQDEMIDCIXXXXXXXXXXXXSRWHTKEATPVRHTRVLXKVACIGAWHPS 849
+ S F Q+EMID I +RW TK H R L KVACIGAWHP+
Sbjct: 205 IKSTFEQNEMIDVIGVTRGKGNEGTTARWGTKRLPRKTH-RGLRKVACIGAWHPA 258
>SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID
complex subunit Taf111|Schizosaccharomyces pombe|chr
1|||Manual
Length = 979
Score = 26.2 bits (55), Expect(2) = 0.97
Identities = 18/51 (35%), Positives = 25/51 (49%)
Frame = +1
Query: 340 TLRPLMDYALC*LSGRSICLKTVDVASTKTGTNARRRLSLKPVRNGRMSLD 492
TL DYA+ S +S+ LK V+ T T RRR + RN + L+
Sbjct: 219 TLEVDTDYAIIFNSKKSLPLKRNVVSPISTHTKKRRRTANTSQRNDGLDLN 269
Score = 21.0 bits (42), Expect(2) = 0.97
Identities = 12/37 (32%), Positives = 17/37 (45%)
Frame = +1
Query: 607 WKSNLTVVPSRTK*NGPENIWRNLSLVDSVFAQDEMI 717
W+ N+ S + + + SLVD FA DE I
Sbjct: 277 WEKNIIYDESDVNKTNQSSFFIDKSLVDIDFAFDENI 313
>SPBC337.03 |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 387
Score = 26.6 bits (56), Expect = 4.8
Identities = 15/59 (25%), Positives = 25/59 (42%)
Frame = -2
Query: 860 NDTLEGCQAPMQATFXKTLVCLTGVASFVCQREXXXXXXXXXXXPMQSIISSWANTEST 684
N E C+ QA +T + S + +++ ++SIIS+ N EST
Sbjct: 198 NKASESCKGNYQACIESRSTLITSLESLIEEQKKLLSEEEESLKSVESIISNLENKEST 256
>SPBC1683.12 |||nicotinic acid plasma membrane transporter
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 482
Score = 26.2 bits (55), Expect = 6.3
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 534 CSVVRVIAHTQMKLLKQRQKKAHIMEIQLNGGTIEDK 644
C+VV + T M+ QRQ K I + LNG +++K
Sbjct: 433 CTVVGTLTATAMRFYLQRQNK--IKQEILNGERVDEK 467
>SPCC794.03 |||amino acid permease, unknown 13|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 554
Score = 25.8 bits (54), Expect = 8.3
Identities = 18/55 (32%), Positives = 25/55 (45%)
Frame = -2
Query: 242 FSNHVGHTGLITDKSSQMNRFARVVFRERLDLTTMTTGPLFGIESHRPMTGC*KF 78
F N VG G+I D S + +R+VF D +G L ++SH T F
Sbjct: 344 FMNVVGILGMIFDTSLSIVASSRLVFAVARDGVLPFSGWLRKVDSHGQPTNAVTF 398
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,664,261
Number of Sequences: 5004
Number of extensions: 76226
Number of successful extensions: 178
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 174
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 450492750
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -