Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_E10
(878 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein S8|Schizosaccha... 187 2e-48
SPAC521.05 |rps802|rps8-2|40S ribosomal protein S8|Schizosacchar... 186 3e-48
SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase ki... 28 1.5
SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16 |S... 28 2.0
SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr 2|... 28 2.0
SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10 |Schizosac... 27 3.5
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 26 6.1
SPAC139.03 |||transcription factor, zf-fungal binuclear cluster ... 26 8.1
SPBC16H5.10c |prp43||ATP-dependent RNA helicase Prp43|Schizosacc... 26 8.1
SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr... 26 8.1
>SPAC2C4.16c |rps801|rps8-1|40S ribosomal protein
S8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 200
Score = 187 bits (455), Expect = 2e-48
Identities = 103/207 (49%), Positives = 129/207 (62%)
Frame = +1
Query: 70 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 249
MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL
Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60
Query: 250 DTGNFSWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLP 429
D+GNFSWGSE ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY +
Sbjct: 61 DSGNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGIL 120
Query: 430 LGRKKGAKLTEAEEAIINKKRSQKTARKYLXKATVLLRLRVLXKSNSTRGVCWLAWRVAQ 609
+G KG K T K Q+ L + V L + V ++ R Q
Sbjct: 121 MG-SKGKKATSTPNP--KSKHVQRKHSARLGDSKVDSALETQFAAGRLYAV--VSSRPGQ 175
Query: 610 VSVVAPMVTSXKAKELEFYLRKIKSKR 690
+ + +EL FYLR++ K+
Sbjct: 176 SGRCDGYI--LEGEELHFYLRRMAPKK 200
Score = 52.4 bits (120), Expect = 8e-08
Identities = 24/38 (63%), Positives = 28/38 (73%)
Frame = +3
Query: 537 AKVEGALXEQFHTGRLLACVASRPGQCGRADGYILXGQ 650
+KV+ AL QF GRL A V+SRPGQ GR DGYIL G+
Sbjct: 150 SKVDSALETQFAAGRLYAVVSSRPGQSGRCDGYILEGE 187
>SPAC521.05 |rps802|rps8-2|40S ribosomal protein
S8|Schizosaccharomyces pombe|chr 1|||Manual
Length = 200
Score = 186 bits (454), Expect = 3e-48
Identities = 88/130 (67%), Positives = 103/130 (79%)
Frame = +1
Query: 70 MGISRDHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 249
MGI+RD HKR ATG KRA RKKRK+ELGR +NTR+GP+RIH VR RGGN K+RALRL
Sbjct: 1 MGITRDSRHKRSATGAKRAQYRKKRKFELGRQPSNTRIGPKRIHEVRVRGGNKKFRALRL 60
Query: 250 DTGNFSWGSECSTRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLP 429
D+GNFSWGSE ++KTRII V Y+ SNNELVRT TL K+AIV +DA PFR WYE+HY +
Sbjct: 61 DSGNFSWGSEGVSKKTRIIQVAYHPSNNELVRTNTLTKSAIVQIDAAPFRVWYETHYGIL 120
Query: 430 LGRKKGAKLT 459
+G KG K T
Sbjct: 121 MG-SKGKKAT 129
Score = 52.4 bits (120), Expect = 8e-08
Identities = 24/38 (63%), Positives = 28/38 (73%)
Frame = +3
Query: 537 AKVEGALXEQFHTGRLLACVASRPGQCGRADGYILXGQ 650
+KV+ AL QF GRL A V+SRPGQ GR DGYIL G+
Sbjct: 150 SKVDSALETQFAAGRLYAVVSSRPGQSGRCDGYILEGE 187
>SPAC1006.09 |win1|SPAC1250.06c, SPAPJ730.01|MAP kinase kinase kinase
Win1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1436
Score = 28.3 bits (60), Expect = 1.5
Identities = 22/76 (28%), Positives = 33/76 (43%), Gaps = 2/76 (2%)
Frame = +1
Query: 82 RDHWHKRRATGGKRAPIRKKRKY--ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRLDT 255
RD +K + G K+ + EL +N + Q+ + S T YRA+ LDT
Sbjct: 1083 RDRLYKSQLIGRVLDDTTKENRLLKELASSKSNITIRWQQGGLIGSGSFGTVYRAVNLDT 1142
Query: 256 GNFSWGSECSTRKTRI 303
G+ E + K RI
Sbjct: 1143 GDLMAVKEVALHKPRI 1158
>SPAC890.03 |ppk16|mug92|serine/threonine protein kinase Ppk16
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 672
Score = 27.9 bits (59), Expect = 2.0
Identities = 26/83 (31%), Positives = 39/83 (46%)
Frame = +1
Query: 163 PAANTRLGPQRIHSVRSRGGNTKYRALRLDTGNFSWGSECSTRKTRIIDVVYNASNNELV 342
P NT + PQ RSR T R+L +G S S ++ +TR V +AS+N L+
Sbjct: 530 PLQNTVISPQPTR--RSR---TPIRSL---SGRSSVASSRNSSRTRSYSNVSSASSNSLI 581
Query: 343 RTKTLVKNAIVVVDATPFRQWYE 411
+ ++ V TPF +E
Sbjct: 582 SIISSKPSSSTVQRQTPFHSPFE 604
>SPBC29A10.12 |||HMG-box variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 207
Score = 27.9 bits (59), Expect = 2.0
Identities = 17/54 (31%), Positives = 26/54 (48%)
Frame = +1
Query: 85 DHWHKRRATGGKRAPIRKKRKYELGRPAANTRLGPQRIHSVRSRGGNTKYRALR 246
+ W K T K+ +KRK L R A RL + + S+ S+GG +A +
Sbjct: 32 EKWSKGVKTN-KKEQEAEKRKAALERKAERERLEKEEMESLPSKGGKGSKKAAK 84
>SPAC31A2.07c |dbp10||ATP-dependent RNA helicase Dbp10
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 848
Score = 27.1 bits (57), Expect = 3.5
Identities = 23/88 (26%), Positives = 33/88 (37%), Gaps = 5/88 (5%)
Frame = +1
Query: 85 DHWHKRRATGGKRAPIRKKRKY-----ELGRPAANTRLGPQRIHSVRSRGGNTKYRALRL 249
D W +A G +PIR+ ++Y + +PA R H R K R + +
Sbjct: 758 DEWKASKAFGANDSPIRENKRYKHNKLQTPKPADKFR---DNYHKQNKRNREAKERGIGI 814
Query: 250 DTGNFSWGSECSTRKTRIIDVVYNASNN 333
N S RK R + A NN
Sbjct: 815 KV-NSELKSAVEIRKARELKEKRLAKNN 841
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 26.2 bits (55), Expect = 6.1
Identities = 15/61 (24%), Positives = 31/61 (50%), Gaps = 2/61 (3%)
Frame = +1
Query: 268 WGSEC--STRKTRIIDVVYNASNNELVRTKTLVKNAIVVVDATPFRQWYESHYTLPLGRK 441
WGS+ +K + +V+ +++ + +T L AT F + +S+Y +PL R+
Sbjct: 250 WGSQSYLQEKKAGLAEVLNISTSQQKPKTTALDYEVFRHEMATKFSSFADSYYPVPLDRE 309
Query: 442 K 444
+
Sbjct: 310 Q 310
>SPAC139.03 |||transcription factor, zf-fungal binuclear cluster
type |Schizosaccharomyces pombe|chr 1|||Manual
Length = 625
Score = 25.8 bits (54), Expect = 8.1
Identities = 15/39 (38%), Positives = 19/39 (48%)
Frame = +1
Query: 58 DPTKMGISRDHWHKRRATGGKRAPIRKKRKYELGRPAAN 174
+ TK G + RRA A RKK K + GRP +N
Sbjct: 3 ETTKSGSKKSGQTSRRAIHSCLACRRKKLKCDHGRPCSN 41
>SPBC16H5.10c |prp43||ATP-dependent RNA helicase
Prp43|Schizosaccharomyces pombe|chr 2|||Manual
Length = 735
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/25 (44%), Positives = 15/25 (60%)
Frame = -1
Query: 407 YHCLNGVASTTTIAFLTRVFVRTNS 333
++C N V S T + + VFVR NS
Sbjct: 518 FYCSNEVLSLTALLSVPNVFVRPNS 542
>SPCC1827.03c |||acetyl-CoA ligase |Schizosaccharomyces pombe|chr
3|||Manual
Length = 512
Score = 25.8 bits (54), Expect = 8.1
Identities = 10/27 (37%), Positives = 17/27 (62%)
Frame = +1
Query: 607 QVSVVAPMVTSXKAKELEFYLRKIKSK 687
Q ++ P+ ++ K E EFY+ +KSK
Sbjct: 75 QRAICGPLNSNYKQSEFEFYIDDLKSK 101
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,060,494
Number of Sequences: 5004
Number of extensions: 58376
Number of successful extensions: 166
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 164
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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