Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_C15
(865 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC354.02c |sec61||translocon alpha subunit Sec61|Schizosacchar... 248 1e-66
SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog |Schi... 129 6e-31
SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces ... 30 0.49
SPCC1450.14c |ero12||ER oxidoreductin Ero1b|Schizosaccharomyces ... 28 1.5
SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase Mok11|S... 27 2.6
SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces pom... 27 2.6
SPBC32H8.13c |mok12||alpha-1,3-glucan synthase Mok12|Schizosacch... 27 3.4
SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr... 27 4.5
SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 26 6.0
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 26 7.9
SPAC8C9.06c |||mitochondrial translation regulator |Schizosaccha... 26 7.9
SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein Rad4|Schizosac... 26 7.9
>SPBC354.02c |sec61||translocon alpha subunit
Sec61|Schizosaccharomyces pombe|chr 2|||Manual
Length = 479
Score = 248 bits (606), Expect = 1e-66
Identities = 112/180 (62%), Positives = 146/180 (81%), Gaps = 1/180 (0%)
Frame = +1
Query: 175 IKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADPF 354
++FL+++KPF LPEIA PERK+ F++K+LWT +TL IFLV Q+PL+GI+SSDS+DP
Sbjct: 4 LRFLDLVKPFAPFLPEIAAPERKVPFKQKMLWTGVTLLIFLVMSQVPLYGIVSSDSSDPL 63
Query: 355 YWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFGM 531
W+R+ILA+NRGTLMELGISPIVTS +++QLL G+++IEV + DR ++ QK +
Sbjct: 64 LWLRMILAANRGTLMELGISPIVTSSMLVQLLVGSQLIEVNMELKSDREMYQLVQKFLAI 123
Query: 532 VITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGISL 711
+I GQA YV+TGMYG P ++GAG+CLL+I+QL A LIVLLLDELLQKGYGLGSGISL
Sbjct: 124 IIAFGQATAYVLTGMYGRPQDLGAGICLLLILQLAAASLIVLLLDELLQKGYGLGSGISL 183
Score = 33.9 bits (74), Expect = 0.030
Identities = 19/37 (51%), Positives = 21/37 (56%)
Frame = +2
Query: 713 FIATNICETIVWKAFSPATGNPWVXAXNSKGAVIXLV 823
FIAT CE I WKAFSP T + +GAVI V
Sbjct: 184 FIATINCENIFWKAFSPTTYH-IANGVQFEGAVINFV 219
>SPBC19G7.17 ||SPBC36B7.01|translocon subunit Sec61 homolog
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 475
Score = 129 bits (311), Expect = 6e-31
Identities = 67/204 (32%), Positives = 119/204 (58%), Gaps = 1/204 (0%)
Frame = +1
Query: 172 GIKFLEVIKPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMSSDSADP 351
G +F+ IKP S+LPE+ P+ ++ EK+ W A + ++ + IP++G +D+ DP
Sbjct: 3 GARFINFIKPLSSLLPEVEGPKTHLELVEKLGWMAGCVVVYQILSIIPVYGAEKTDTLDP 62
Query: 352 FYWIRVILASNRGTLMELGISPIVTSGLIMQLLAGAKIIEVG-DTPKDRALFNGAQKLFG 528
RV+ S+ LM G++PI S ++Q+LA K I V + DR LF AQK+
Sbjct: 63 INNFRVLDGSSASGLMITGLAPIYLSSFLLQILASKKKIAVNFNLIIDRVLFQNAQKVVS 122
Query: 529 MVITVGQAIVYVMTGMYGEPSEIGAGVCLLIIIQLFVAGLIVLLLDELLQKGYGLGSGIS 708
++ + A+ YV +G YG S++G +++I+Q+F+ G++ + L E+++KG+GLGSG
Sbjct: 123 ALLYLILAVTYVSSGYYGSFSDLGIFRFIMLILQIFLPGIVCIYLCEIIEKGHGLGSG-P 181
Query: 709 LVYCYKHL*NHCMEGIFTSHW*PL 780
++ H+ + M + + H P+
Sbjct: 182 VLLLGSHILGNIMWDVLSLHRYPV 205
>SPAC589.05c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 346
Score = 29.9 bits (64), Expect = 0.49
Identities = 23/67 (34%), Positives = 30/67 (44%), Gaps = 4/67 (5%)
Frame = -3
Query: 521 NFCAPLNKALSFGVSPTS-MILAPASNCMISPDVTIGEIPSSINV---PLFDARITRIQ* 354
+ CA +NKAL G+ TS A C PD I + S V PL + RI ++
Sbjct: 104 SLCAAINKALDAGIPITSPAFYADEKQC---PDTLIASVFDSATVEKIPLLEERIRIMRA 160
Query: 353 KGSALSD 333
G L D
Sbjct: 161 SGRVLVD 167
>SPCC1450.14c |ero12||ER oxidoreductin Ero1b|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 571
Score = 28.3 bits (60), Expect = 1.5
Identities = 12/51 (23%), Positives = 23/51 (45%)
Frame = +2
Query: 698 LVSLWFIATNICETIVWKAFSPATGNPWVXAXNSKGAVIXLVPSGGQXVQN 850
LV L + N+C+ + S +G+PW + K + + S ++N
Sbjct: 11 LVGLLILYKNVCQVLCKSGSSVKSGSPWSIKNDGKSIIHDTLNSSYSDIEN 61
>SPAC1527.01 |mok11|SPAC23D3.15|alpha-1,3-glucan synthase
Mok11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2397
Score = 27.5 bits (58), Expect = 2.6
Identities = 17/66 (25%), Positives = 31/66 (46%), Gaps = 3/66 (4%)
Frame = -2
Query: 711 QRDTRTQSISFLEQ---FIEQQHNKTGHKQLYDNEEAHSGTNFTGFAIHSSHYINNSLTN 541
Q+ +S S ++ F E Q++ TG+K + A +G + G + +S +
Sbjct: 1700 QKSPSVESFSIIDNDNPFHEGQNSSTGYKDIVQGLLAENGVSEAGVDVMTSIVSSTIPIV 1759
Query: 540 SNHHTK 523
SNH T+
Sbjct: 1760 SNHQTE 1765
>SPBC1711.08 |||chaperone activator Aha1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 336
Score = 27.5 bits (58), Expect = 2.6
Identities = 16/42 (38%), Positives = 22/42 (52%), Gaps = 1/42 (2%)
Frame = -2
Query: 648 KTGHKQLYDNEEAHSGTNFTGFAIHSSHYINN-SLTNSNHHT 526
+T +Y + E H+G G +HSS NN S T+SN T
Sbjct: 144 QTHGDDVYLSTEEHNGNAARGLPVHSSFKQNNSSQTSSNKGT 185
>SPBC32H8.13c |mok12||alpha-1,3-glucan synthase
Mok12|Schizosaccharomyces pombe|chr 2|||Manual
Length = 2352
Score = 27.1 bits (57), Expect = 3.4
Identities = 18/54 (33%), Positives = 27/54 (50%), Gaps = 1/54 (1%)
Frame = +1
Query: 175 IKFLEVI-KPFCSILPEIAKPERKIQFREKVLWTAITLFIFLVCCQIPLFGIMS 333
I+FL+ KP+ S + R++ + K WT + I L+ PLFGI S
Sbjct: 1055 IRFLDPPPKPYLSYKMYLNDFTRQLHYIPKGSWTVQIVAIVLLILLPPLFGIFS 1108
>SPAC4G8.03c |||RNA-binding protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 780
Score = 26.6 bits (56), Expect = 4.5
Identities = 13/41 (31%), Positives = 23/41 (56%)
Frame = -3
Query: 713 TREIPEPNPYPFWSNSSSNNTIRPATNSCMIMRRHTPAPIS 591
+RE+ N PF SNS S ++ T++ +R + +PI+
Sbjct: 33 SRELDYANQSPFSSNSFSPTELKARTSATFDVRSASTSPIN 73
>SPAC458.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 340
Score = 26.2 bits (55), Expect = 6.0
Identities = 17/61 (27%), Positives = 29/61 (47%)
Frame = -3
Query: 401 SINVPLFDARITRIQ*KGSALSDDIIPNKGIWQHTKKINNVIAVHNTFSRN*ILRSGFAI 222
S+ + LFD I+ + S +SD IP K +++ N + T+S N + +S
Sbjct: 273 SLLLGLFDKSISMLNCHSSKISDGDIPYKSHQVFLQELQNKYSEIKTYSSNKLNKSSIKS 332
Query: 221 S 219
S
Sbjct: 333 S 333
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 25.8 bits (54), Expect = 7.9
Identities = 10/18 (55%), Positives = 13/18 (72%)
Frame = -3
Query: 704 IPEPNPYPFWSNSSSNNT 651
+P P P P +NSS+NNT
Sbjct: 398 MPPPAPRPVTNNSSNNNT 415
>SPAC8C9.06c |||mitochondrial translation regulator
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 931
Score = 25.8 bits (54), Expect = 7.9
Identities = 21/75 (28%), Positives = 32/75 (42%)
Frame = -2
Query: 582 AIHSSHYINNSLTNSNHHTK*FLCTIKQSPVFRSVTNFNDFSTSKQLHDKS*CYNR*DTK 403
A SH N+ N + T+ +S V R+V ND + KS C+ D++
Sbjct: 32 AFQQSHTFNSMSVFKNDNAIANQTTVNESDVKRNVEKINDIYECSN-NTKSPCFPNSDSR 90
Query: 402 LH*CPSV*CKNYTDP 358
+ P V K T+P
Sbjct: 91 I---PLVNWKTSTNP 102
>SPAC23C4.18c |rad4|cut5, dre3|BRCT domain protein
Rad4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 648
Score = 25.8 bits (54), Expect = 7.9
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = -3
Query: 224 ISGNILQNGLITSKNFIPILIYC 156
+ G L +GL+ K+F+P L C
Sbjct: 83 VQGEDLDDGLLVDKHFLPTLFKC 105
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,630,551
Number of Sequences: 5004
Number of extensions: 79996
Number of successful extensions: 230
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 214
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 228
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 430470850
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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