Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP13_F_B07
(794 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|ch... 52 7e-08
SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces pomb... 44 2e-05
SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces... 43 4e-05
SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr 3|||Ma... 36 0.007
SPBC215.01 ||SPBC3B9.20|GTPase activating protein|Schizosaccharo... 27 2.3
SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr 2... 27 3.1
SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces ... 26 7.1
SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3 |Schizosacchar... 25 9.4
>SPAC3A12.14 |cam1||calmodulin Cam1 |Schizosaccharomyces pombe|chr
1|||Manual
Length = 150
Score = 52.4 bits (120), Expect = 7e-08
Identities = 29/78 (37%), Positives = 44/78 (56%)
Frame = +1
Query: 322 TAKQRKTKTRAAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCM 501
T RK K E+ E K++DK+ NG + ELTH L +LGE+L EVA++ ++
Sbjct: 72 TMMARKMKDTDNEEEVREAFKVFDKDGNGYITVEELTHVLTSLGERLSQEEVADMIREA- 130
Query: 502 DPEXDDGMIPYAAFLKKV 555
D + DG+I Y F + +
Sbjct: 131 DTD-GDGVINYEEFSRVI 147
Score = 28.3 bits (60), Expect = 1.3
Identities = 18/68 (26%), Positives = 38/68 (55%)
Frame = +1
Query: 364 DFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEXDDGMIPYAAF 543
+F E L+D++++G + EL + +LG+ +E+ ++ + +D + +G I + F
Sbjct: 13 EFREAFSLFDRDQDGNITSNELGVVMRSLGQSPTAAELQDMINE-VDAD-GNGTIDFTEF 70
Query: 544 LKKVMAXK 567
L +MA K
Sbjct: 71 L-TMMARK 77
>SPAP8A3.08 |cdc4||myosin II light chain|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 141
Score = 44.4 bits (100), Expect = 2e-05
Identities = 22/67 (32%), Positives = 41/67 (61%)
Frame = +1
Query: 361 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEXDDGMIPYAA 540
E+F++ +++DK+ G++ EL + L +LGEKL + E+ E+ K DGM+ Y
Sbjct: 77 EEFVKGFQVFDKDATGMIGVGELRYVLTSLGEKLSNEEMDELLKGV---PVKDGMVNYHD 133
Query: 541 FLKKVMA 561
F++ ++A
Sbjct: 134 FVQMILA 140
Score = 25.8 bits (54), Expect = 7.1
Identities = 11/19 (57%), Positives = 14/19 (73%)
Frame = +3
Query: 198 STLXDLLRALNSNPTLATI 254
+++ DLLRA NPTLA I
Sbjct: 26 TSIGDLLRACGQNPTLAEI 44
>SPAC29A4.05 |cam2||myosin I light chain Cam2|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 143
Score = 43.2 bits (97), Expect = 4e-05
Identities = 21/75 (28%), Positives = 44/75 (58%)
Frame = +1
Query: 337 KTKTRAAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEXD 516
K + + E++++ +++DK+ +G + A+ + LGEKL D+EV + ++ DP +
Sbjct: 70 KLRETESEEEYIKAFRVFDKDNSGYIETAKFADYMKTLGEKLSDNEVQLMVQEA-DP-TN 127
Query: 517 DGMIPYAAFLKKVMA 561
G Y F++++MA
Sbjct: 128 SGSFDYYDFVQRIMA 142
Score = 28.7 bits (61), Expect = 1.0
Identities = 13/48 (27%), Positives = 29/48 (60%)
Frame = +1
Query: 361 EDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMD 504
++ E LYD +++GL+ + + L +LG + D+E+A+++ + D
Sbjct: 9 DEMKEAFVLYDIDKDGLIPTSHVGSVLRSLGINVTDAELAKLSNELGD 56
>SPCC1682.04 |cdc31||centrin|Schizosaccharomyces pombe|chr
3|||Manual
Length = 176
Score = 35.9 bits (79), Expect = 0.007
Identities = 21/74 (28%), Positives = 37/74 (50%)
Frame = +1
Query: 274 RRRARSCSHSKSSFPSTAKQRKTKTRAAYEDFLECLKLYDKNENGLMLGAELTHTLLALG 453
+RR+R+ S + + A R T +D E KL+D +++ + EL + ALG
Sbjct: 8 KRRSRASSPTPARLGGYAPLRVEITEEQRQDINEAFKLFDSDKDNAIDYHELRAAMRALG 67
Query: 454 EKLDDSEVAEVTKD 495
+ SEV ++ +D
Sbjct: 68 FNAEKSEVLKILRD 81
Score = 25.8 bits (54), Expect = 7.1
Identities = 19/74 (25%), Positives = 32/74 (43%)
Frame = +1
Query: 337 KTKTRAAYEDFLECLKLYDKNENGLMLGAELTHTLLALGEKLDDSEVAEVTKDCMDPEXD 516
K R E+ +L+D +E G + L L E +DD E+ + ++ D +
Sbjct: 102 KIVERDPLEEIKRAFELFDDDETGKISLRNLRRVAKELNENIDDQELEAMIEE-FDLD-Q 159
Query: 517 DGMIPYAAFLKKVM 558
DG I F+ +M
Sbjct: 160 DGEINEQEFIAIMM 173
>SPBC215.01 ||SPBC3B9.20|GTPase activating
protein|Schizosaccharomyces pombe|chr 2|||Manual
Length = 834
Score = 27.5 bits (58), Expect = 2.3
Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 1/39 (2%)
Frame = +2
Query: 110 MSDLSKNDVER-ASFAFSIYDFEGKGKIDAFNLXRSPES 223
+++L DV R SF F +YDF G G +D ++ + E+
Sbjct: 605 IAELKFRDVMRNISFIFELYDFNGDGFMDKPDVLKVSEA 643
>SPBC800.13 |||histone H4 variant|Schizosaccharomyces pombe|chr
2|||Manual
Length = 479
Score = 27.1 bits (57), Expect = 3.1
Identities = 18/61 (29%), Positives = 30/61 (49%), Gaps = 3/61 (4%)
Frame = +3
Query: 27 REFLKIXTSCLERLPVKTGREPE---KHHTK*ATSARTTLKGRLSPSQSTTLKAKARSMP 197
R+ L++ + L + PV + E E + HT S ++ RLS ++ TLK A+
Sbjct: 72 RDILRMLSRALAKNPVPSPAESESSERRHTPQTNSQKSQKTPRLSSNKRRTLKNDAKQRN 131
Query: 198 S 200
S
Sbjct: 132 S 132
>SPAPB17E12.14c |||6-phosphofructo-2-kinase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 474
Score = 25.8 bits (54), Expect = 7.1
Identities = 14/48 (29%), Positives = 27/48 (56%), Gaps = 1/48 (2%)
Frame = +1
Query: 274 RRRARSCSHSKSSFPSTAKQRKTKTRAAYE-DFLECLKLYDKNENGLM 414
RR+ S SH +SF + + +K R ++ D L+ L + + E+G++
Sbjct: 36 RRKHASHSHDDASFFDPSNEEASKLRESFAMDTLDALLQWFEEEDGVV 83
>SPCC18.01c |adg3|SPCC74.07c|beta-glucosidase Adg3
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1131
Score = 25.4 bits (53), Expect = 9.4
Identities = 17/60 (28%), Positives = 26/60 (43%)
Frame = -3
Query: 225 ALSGDRXRLKASILPLPSKS*IEKAKDALSTSFLLRSLILCGVFRVPSRSSLADVQDKKS 46
+LS + S +P S S + +ALS++ L S +PS SS +V S
Sbjct: 536 SLSSSTSSVSTSYIPNASSSVYASSTEALSSNSLSSSTSSASTSYIPSASSSYEVASNSS 595
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,617,130
Number of Sequences: 5004
Number of extensions: 44728
Number of successful extensions: 128
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 122
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 128
length of database: 2,362,478
effective HSP length: 71
effective length of database: 2,007,194
effective search space used: 387388442
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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