Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP13_F_A23
         (889 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   111   2e-25
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   107   2e-24
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    48   1e-06
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    30   0.38 
SPAC9G1.04 |oxa101|oxa1, oxa1-1, oxa1sp1|mitochondrial inner mem...    27   2.7  
SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomy...    26   8.2  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  111 bits (266), Expect = 2e-25
 Identities = 66/143 (46%), Positives = 84/143 (58%), Gaps = 4/143 (2%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCL--GARHPALMARCPQTRPSGGGDDSFNTFFQRDR 323
           MRE IS+HVGQAG QIGNACWELYCL  G +    M   P+T  S   D  F+TFF    
Sbjct: 1   MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMN--PET-ASQNSDGGFSTFFSETG 57

Query: 324 SWQHVPPWLSSSILKLLL--FE*GPHWAHTDSCFIQKXLITGKERCGPTTMPRGHYTIGK 497
             ++VP  +   +   ++     GP+       F  + LITGKE        RGHYT+GK
Sbjct: 58  QGKYVPRSIYVDLEPNVIDQVRTGPY----RDLFHPEQLITGKEDAS-NNYARGHYTVGK 112

Query: 498 EIVDLVLDRIRKLADQCTGLQGF 566
           E+VD V D+IR++AD C+GLQGF
Sbjct: 113 ELVDEVTDKIRRIADNCSGLQGF 135



 Score = 97.5 bits (232), Expect = 2e-21
 Identities = 72/186 (38%), Positives = 93/186 (50%), Gaps = 3/186 (1%)
 Frame = +2

Query: 341 PVAVFVDLETTVV*MRSALGTYRQLFHPETXYYW*GKMRA-NNYAPWSLHHWKGNRRFGF 517
           P +++VDLE  V+  +   G YR LFHPE      GK  A NNYA    H+  G      
Sbjct: 63  PRSIYVDLEPNVI-DQVRTGPYRDLFHPEQLIT--GKEDASNNYARG--HYTVGKELVDE 117

Query: 518 GQNPQAR*PVYRSAR--ILIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXIYPAPXV 691
             +   R     S     L+FH            LL+ERL+++Y KKSKL+F +YPAP V
Sbjct: 118 VTDKIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQV 177

Query: 692 STAVVEPYXSILTXHTTLEHSDCAFMGRQ*SHL*HLAAVISXLSAQPKPX*NRXIGQIVP 871
           ST+VVEPY S+LT H TL+ +DC FM    S    +      +        NR I Q+V 
Sbjct: 178 STSVVEPYNSVLTTHATLDLADCTFMVDNESCY-DICRRNLDIERPSYENLNRLIAQVVS 236

Query: 872 SITGXL 889
           SIT  L
Sbjct: 237 SITASL 242


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  107 bits (257), Expect = 2e-24
 Identities = 62/144 (43%), Positives = 81/144 (56%), Gaps = 5/144 (3%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCL--GARHPALMARCPQTRPSGGG-DDSFNTFFQRD 320
           MRE ISVHVGQAGVQIGNACWELYCL  G           +   +    +D F TFF   
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHKNNSYLNDGFGTFFSET 60

Query: 321 RSWQHVPPWLSSSILKLLL--FE*GPHWAHTDSCFIQKXLITGKERCGPTTMPRGHYTIG 494
              + VP  +   +   ++     GP+       F  + ++TGKE        RGHYT+G
Sbjct: 61  GQGKFVPRSIYVDLEPNVIDQVRTGPY----KDLFHPEQMVTGKEDAS-NNYARGHYTVG 115

Query: 495 KEIVDLVLDRIRKLADQCTGLQGF 566
           KE++D VL+RIR++AD C+GLQGF
Sbjct: 116 KEMIDSVLERIRRMADNCSGLQGF 139



 Score = 99.1 bits (236), Expect = 7e-22
 Identities = 71/186 (38%), Positives = 93/186 (50%), Gaps = 3/186 (1%)
 Frame = +2

Query: 341 PVAVFVDLETTVV*MRSALGTYRQLFHPETXYYW*GKMRA-NNYAPWSLHHWKGNRRFGF 517
           P +++VDLE  V+  +   G Y+ LFHPE      GK  A NNYA    H+  G      
Sbjct: 67  PRSIYVDLEPNVI-DQVRTGPYKDLFHPEQMVT--GKEDASNNYARG--HYTVGKEMIDS 121

Query: 518 GQNPQAR*PVYRSAR--ILIFHXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXIYPAPXV 691
                 R     S     L+FH            LL+ERL+++YGKKS L+F +YPAP V
Sbjct: 122 VLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQV 181

Query: 692 STAVVEPYXSILTXHTTLEHSDCAFMGRQ*SHL*HLAAVISXLSAQPKPX*NRXIGQIVP 871
           ST+VVEPY S+LT H TL++SDC FM    +    +      +        NR I Q+V 
Sbjct: 182 STSVVEPYNSVLTTHATLDNSDCTFMVDNEACY-DICRRNLDIERPTYENLNRLIAQVVS 240

Query: 872 SITGXL 889
           SIT  L
Sbjct: 241 SITASL 246


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 48.4 bits (110), Expect = 1e-06
 Identities = 38/139 (27%), Positives = 57/139 (41%)
 Frame = +3

Query: 150 MRECISVHVGQAGVQIGNACWELYCLGARHPALMARCPQTRPSGGGDDSFNTFFQRDRSW 329
           MRE + +  GQ G Q+G A W      A    L +       S    +  N +F      
Sbjct: 1   MREIVHIQAGQCGNQVGAAFWSTI---ADEHGLDSAGIYHGTSEAQHERLNVYFNEAAGG 57

Query: 330 QHVPPWLSSSILKLLLFE*GPHWAHTDSCFIQKXLITGKERCGPTTMPRGHYTIGKEIVD 509
           ++VP  +   +    +           + F    +I G+   G     +GHYT G E+ D
Sbjct: 58  KYVPRAVLVDLEPGTMD--AVKSGKFGNLFRPDNIIYGQSGAG-NIWAKGHYTEGAELAD 114

Query: 510 LVLDRIRKLADQCTGLQGF 566
            VLD +R+ A+ C  LQGF
Sbjct: 115 AVLDVVRREAEACDALQGF 133



 Score = 45.2 bits (102), Expect = 1e-05
 Identities = 41/146 (28%), Positives = 60/146 (41%), Gaps = 4/146 (2%)
 Frame = +2

Query: 341 PVAVFVDLET-TVV*MRSALGTYRQLFHPETXYYW*GKMRANNYAPWSLHHWKGNRRFGF 517
           P AV VDLE  T+  ++S  G +  LF P+   Y  G+  A N   W+  H+        
Sbjct: 61  PRAVLVDLEPGTMDAVKS--GKFGNLFRPDNIIY--GQSGAGNI--WAKGHYTEGAELAD 114

Query: 518 GQNPQAR*PVYRSARILIF---HXXXXXXXXXXXXLLMERLSVDYGKKSKLEFXIYPAPX 688
                 R        +  F   H            LL+ ++  +Y  +    F + PAP 
Sbjct: 115 AVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPK 174

Query: 689 VSTAVVEPYXSILTXHTTLEHSDCAF 766
            S  VVEPY + L+ H  +E+SD  F
Sbjct: 175 SSDTVVEPYNATLSMHQLVENSDETF 200


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 30.3 bits (65), Expect = 0.38
 Identities = 12/25 (48%), Positives = 16/25 (64%)
 Frame = +3

Query: 153 RECISVHVGQAGVQIGNACWELYCL 227
           RE I++  GQ G QIG+  W+  CL
Sbjct: 3   REIITLQAGQCGNQIGSQFWQQLCL 27


>SPAC9G1.04 |oxa101|oxa1, oxa1-1, oxa1sp1|mitochondrial inner
           membrane translocase Oxa101|Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 374

 Score = 27.5 bits (58), Expect = 2.7
 Identities = 20/60 (33%), Positives = 26/60 (43%), Gaps = 4/60 (6%)
 Frame = +2

Query: 191 PDR*CLLGALLPGSTASSPD-GQMPTDQTIGG---WRRFFQHFLSARPELAARTPVAVFV 358
           PD  C+L  +  G   S     +  T  TIG    WR FF       P L A+ P A+F+
Sbjct: 205 PDPYCILPIINAGLMFSGMQMNRANTASTIGNSTNWRTFFFLCCLLSPLLTAKLPAAIFM 264


>SPAC29E6.06c ||SPAC30.10c|cysteine-tRNA ligase |Schizosaccharomyces
           pombe|chr 1|||Manual
          Length = 754

 Score = 25.8 bits (54), Expect = 8.2
 Identities = 13/35 (37%), Positives = 16/35 (45%), Gaps = 2/35 (5%)
 Frame = -3

Query: 281 PRWSGLWASGHQGW-MPCS-QAVKLPAGITDLDSG 183
           P W   W+ G  GW + CS  A  L     D+ SG
Sbjct: 333 PSWDSPWSKGRPGWHIECSVMASALLGSNIDIHSG 367


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,528,243
Number of Sequences: 5004
Number of extensions: 71609
Number of successful extensions: 165
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 161
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)