Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_N23
(1045 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 31 0.36
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.36
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.47
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.82
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.5
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 27 3.3
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 27 4.4
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 27 5.8
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 30.7 bits (66), Expect = 0.36
Identities = 21/71 (29%), Positives = 22/71 (30%), Gaps = 7/71 (9%)
Frame = -1
Query: 469 GPPRPGXAXXXGGXPXPPPP-----XGXRPXPPXGTGXPXTGPP--XXX*GXXAXPEAGX 311
G P G P PPPP G P PP G P PP G P +
Sbjct: 323 GKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSSS 382
Query: 310 XXXQGPPGXXP 278
PP P
Sbjct: 383 RAVSNPPAPPP 393
Score = 26.2 bits (55), Expect = 7.6
Identities = 19/65 (29%), Positives = 21/65 (32%), Gaps = 8/65 (12%)
Frame = -1
Query: 427 PXPPPPXGXRPXPPXGTG-----XPXTGPPXXX*GXXA---XPEAGXXXXQGPPGXXPXX 272
P PPP R PP G G P PP + P+ PP P
Sbjct: 313 PPPPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPST 372
Query: 271 GXXPP 257
G PP
Sbjct: 373 GRQPP 377
Score = 26.2 bits (55), Expect = 7.6
Identities = 21/71 (29%), Positives = 22/71 (30%)
Frame = -1
Query: 463 PRPGXAXXXGGXPXPPPPXGXRPXPPXGTGXPXTGPPXXX*GXXAXPEAGXXXXQGPPGX 284
P G A P P PP PP P + PP G A P PP
Sbjct: 404 PPLGNASRTSTPPVPTPPSLPPSAPP---SLPPSAPPSLPMGAPAAPPLPPSAPIAPP-L 459
Query: 283 XPXXGXXPPLP 251
PPLP
Sbjct: 460 PAGMPAAPPLP 470
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 30.7 bits (66), Expect = 0.36
Identities = 16/37 (43%), Positives = 16/37 (43%)
Frame = -1
Query: 463 PRPGXAXXXGGXPXPPPPXGXRPXPPXGTGXPXTGPP 353
P P A GG P PPPP G G G P PP
Sbjct: 750 PVPPPAPIMGGPPPPPPPPGV-----AGAGPPPPPPP 781
Score = 29.5 bits (63), Expect = 0.82
Identities = 17/49 (34%), Positives = 17/49 (34%), Gaps = 1/49 (2%)
Frame = -3
Query: 422 PPPPXGGPXXPAPGDGXPXHXPPXPXXRG-XGPPRSGXVXXPGPPRLXP 279
PPPP P P PP P G PP V GPP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPP 780
Score = 28.7 bits (61), Expect = 1.4
Identities = 11/22 (50%), Positives = 11/22 (50%)
Frame = -1
Query: 475 GGGPPRPGXAXXXGGXPXPPPP 410
GG PP P G P PPPP
Sbjct: 759 GGPPPPPPPPGVAGAGPPPPPP 780
Score = 28.3 bits (60), Expect = 1.9
Identities = 13/30 (43%), Positives = 13/30 (43%)
Frame = +2
Query: 851 PPXXXAXXPGXPRPXPPGRXXPXPXPPXPP 940
PP P P P PPG P PP PP
Sbjct: 753 PPAPIMGGPPPP-PPPPGVAGAGPPPPPPP 781
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.3 bits (65), Expect = 0.47
Identities = 25/74 (33%), Positives = 26/74 (35%)
Frame = -1
Query: 472 GGPPRPGXAXXXGGXPXPPPPXGXRPXPPXGTGXPXTGPPXXX*GXXAXPEAGXXXXQGP 293
G PP P A G P PPP P P G P PP P AG P
Sbjct: 1177 GIPPVPKPA---AGVPPVPPPSEAPPVPKPSVGVPPVPPPSTA-PPVPTPSAGL-----P 1227
Query: 292 PGXXPXXGXXPPLP 251
P P PP+P
Sbjct: 1228 PVPVP-TAKAPPVP 1240
Score = 29.1 bits (62), Expect = 1.1
Identities = 23/78 (29%), Positives = 25/78 (32%), Gaps = 4/78 (5%)
Frame = -1
Query: 472 GGPPRPGXAXXXGGXPXPPPPXGXRPXPPXGTGXPXTGPPXXX*GXXAXPEAG----XXX 305
G PP P + P P P G P P G P PP P G
Sbjct: 1157 GAPPVPKPSV--AAPPVPAPSSGIPPVPKPAAGVPPVPPPSEA-PPVPKPSVGVPPVPPP 1213
Query: 304 XQGPPGXXPXXGXXPPLP 251
PP P G PP+P
Sbjct: 1214 STAPPVPTPSAG-LPPVP 1230
Score = 27.9 bits (59), Expect = 2.5
Identities = 14/44 (31%), Positives = 15/44 (34%)
Frame = -3
Query: 419 PPPXGGPXXPAPGDGXPXHXPPXPXXRGXGPPRSGXVXXPGPPR 288
PPP P P P PP P G P V P P+
Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPK 1106
Score = 27.5 bits (58), Expect = 3.3
Identities = 22/78 (28%), Positives = 25/78 (32%), Gaps = 4/78 (5%)
Frame = -1
Query: 472 GGPPRPGXAXXXGGXPXPPPPXGXRPXPPXGTGXPXTGPPXXX*GXXAXPEAG----XXX 305
G PP P + P P P G P P P P P AG
Sbjct: 1138 GAPPVPKPSV---AAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPP 1194
Query: 304 XQGPPGXXPXXGXXPPLP 251
+ PP P G PP+P
Sbjct: 1195 SEAPPVPKPSVG-VPPVP 1211
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.5 bits (63), Expect = 0.82
Identities = 24/72 (33%), Positives = 24/72 (33%), Gaps = 3/72 (4%)
Frame = +3
Query: 252 GXGGXXPXXGXKPGGPWXXHXPASGXAXXPXXXXGGP--VXGXPVP-XGGXGRXPXGGGG 422
G GG G GG H G P GGP G P GG G G GG
Sbjct: 203 GPGGFGGFGGF--GGEGHHHGGHGGFGGGPGGFEGGPGGFGGGPGGFGGGLGGFGGGPGG 260
Query: 423 XGXPPXXXAXPG 458
G P PG
Sbjct: 261 FGGGPGGHGGPG 272
Score = 28.7 bits (61), Expect = 1.4
Identities = 20/57 (35%), Positives = 20/57 (35%)
Frame = +1
Query: 253 GXGXPPXXXGXSRGGPGXXTXPLRGGPXPLXXGXGGXCXGXPSPGAGXXGPPXGGGG 423
G G G GGPG GGP G GG G G G G G GG
Sbjct: 215 GEGHHHGGHGGFGGGPGG----FEGGPGGFGGGPGGFGGGLGGFGGGPGGFGGGPGG 267
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.5
Identities = 16/39 (41%), Positives = 16/39 (41%), Gaps = 1/39 (2%)
Frame = -1
Query: 466 PPRP-GXAXXXGGXPXPPPPXGXRPXPPXGTGXPXTGPP 353
P RP A P PPPP P PP P GPP
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMP-AGPP 1728
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 27.5 bits (58), Expect = 3.3
Identities = 17/63 (26%), Positives = 18/63 (28%)
Frame = -3
Query: 422 PPPPXGGPXXPAPGDGXPXHXPPXPXXRGXGPPRSGXVXXPGPPRLXPXXXGGXPXPXRX 243
PP P P PA P PP P PP PP P P +
Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIP--SSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKV 201
Query: 242 XXP 234
P
Sbjct: 202 PPP 204
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.1 bits (57), Expect = 4.4
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 884 PRPXPPGRXXPXPXPPXPP 940
P P PPG P PP PP
Sbjct: 11 PPPPPPGFEPPSQPPPPPP 29
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 26.6 bits (56), Expect = 5.8
Identities = 15/33 (45%), Positives = 15/33 (45%), Gaps = 1/33 (3%)
Frame = -2
Query: 942 RGGXGGXGXGXXRPGGXGRGXP-GXXAXXXGGR 847
RGG G G G R G GRG G GGR
Sbjct: 32 RGGARGGGRGGARGGRGGRGGARGGRGGSSGGR 64
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,505,598
Number of Sequences: 5004
Number of extensions: 20367
Number of successful extensions: 165
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 36
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 547228964
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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