BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_N12
(960 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.24
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.97
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 0.97
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.3
SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein Cap1|S... 27 3.9
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 27 3.9
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 3.9
SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces... 27 3.9
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 27 3.9
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 5.2
SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr 2... 27 5.2
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.9
SPBC83.01 |ucp8||UBA/EH/EF hand domain protein Ucp8|Schizosaccha... 26 9.1
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.1 bits (67), Expect = 0.24
Identities = 12/31 (38%), Positives = 14/31 (45%)
Frame = +1
Query: 769 PXTPPPAXXTHPXPPPPXXXPPPXXHXPPXP 861
P + P + P PPPP PP PP P
Sbjct: 1694 PQSAAPPQMSAPTPPPPPMSVPPPPSAPPMP 1724
Score = 30.7 bits (66), Expect = 0.32
Identities = 14/31 (45%), Positives = 15/31 (48%), Gaps = 2/31 (6%)
Frame = +1
Query: 775 TPPPAXXTHPXPP--PPXXXPPPXXHXPPXP 861
TPPP + P PP PP PP PP P
Sbjct: 1706 TPPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.1 bits (62), Expect = 0.97
Identities = 13/34 (38%), Positives = 13/34 (38%)
Frame = +1
Query: 784 PAXXTHPXPPPPXXXPPPXXHXPPXPXXXX*XKN 885
P P PPPP PP PP P KN
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPPGYVKKRKN 38
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 9 PPPPPPPP 16
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.1 bits (62), Expect = 0.97
Identities = 20/49 (40%), Positives = 20/49 (40%)
Frame = -2
Query: 914 GGGGGXPGWXFXXXXXXXGXGGXXLXGGGXXXGGGGXGWVXXAGGGVXG 768
GG GG PG F G GG GGG GGG G GG G
Sbjct: 224 GGFGGGPG-GFEGGPGGFG-GGPGGFGGGLGGFGGGPGGFGGGPGGHGG 270
Score = 26.6 bits (56), Expect = 5.2
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = -3
Query: 883 FXXXXGXXGGGGGXXXGXGXXGGGGGXGG 797
F G GGG G G G G GGG GG
Sbjct: 233 FEGGPGGFGGGPGGFGG-GLGGFGGGPGG 260
Score = 25.8 bits (54), Expect = 9.1
Identities = 14/29 (48%), Positives = 14/29 (48%), Gaps = 5/29 (17%)
Frame = -3
Query: 868 GXXGGGGGXXXGXGXXGGG-----GGXGG 797
G GG GG G G GGG GG GG
Sbjct: 239 GFGGGPGGFGGGLGGFGGGPGGFGGGPGG 267
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.7 bits (61), Expect = 1.3
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 460 PGGGGXXXAPPPPPPP 413
PG G PPPPPPP
Sbjct: 768 PGVAGAGPPPPPPPPP 783
Score = 28.3 bits (60), Expect = 1.7
Identities = 14/39 (35%), Positives = 14/39 (35%)
Frame = -3
Query: 526 PPXXPGXXCXVPXXXPHXXSXRPGGGGXXXAPPPPPPPP 410
PP P P P P PPPPPPPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPV---PPPAPIMGGPPPPPPPP 768
Score = 28.3 bits (60), Expect = 1.7
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = -3
Query: 460 PGGGGXXXAPPPPPPPP 410
P G PPPPPPPP
Sbjct: 767 PPGVAGAGPPPPPPPPP 783
Score = 27.9 bits (59), Expect = 2.2
Identities = 14/40 (35%), Positives = 14/40 (35%)
Frame = -3
Query: 532 LXPPXXPGXXCXVPXXXPHXXSXRPGGGGXXXAPPPPPPP 413
L P P VP P P PPPPPPP
Sbjct: 729 LKSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 27.9 bits (59), Expect = 2.2
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = -3
Query: 460 PGGGGXXXAPPPPPPPP 410
P G PPPPPPPP
Sbjct: 766 PPPGVAGAGPPPPPPPP 782
Score = 27.5 bits (58), Expect = 3.0
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +1
Query: 769 PXTPPPAXXTHPXPPPPXXXPP 834
P PPP PPPP PP
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPP 783
Score = 26.6 bits (56), Expect = 5.2
Identities = 13/33 (39%), Positives = 13/33 (39%), Gaps = 2/33 (6%)
Frame = +1
Query: 769 PXTPPPAXXTHPXPPPPXXXPP--PXXHXPPXP 861
P PPPA P P PP P PP P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP 764
Score = 26.2 bits (55), Expect = 6.9
Identities = 10/17 (58%), Positives = 10/17 (58%)
Frame = -3
Query: 460 PGGGGXXXAPPPPPPPP 410
P G A PPPPPPP
Sbjct: 765 PPPPGVAGAGPPPPPPP 781
Score = 25.8 bits (54), Expect = 9.1
Identities = 13/29 (44%), Positives = 13/29 (44%)
Frame = +1
Query: 775 TPPPAXXTHPXPPPPXXXPPPXXHXPPXP 861
TP PA P P P PPP PP P
Sbjct: 743 TPAPAPIPVPPPAPIMGGPPP---PPPPP 768
>SPCC306.09c |cap1|cap|adenylyl cyclase-associated protein
Cap1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 551
Score = 27.1 bits (57), Expect = 3.9
Identities = 9/16 (56%), Positives = 9/16 (56%)
Frame = -3
Query: 457 GGGGXXXAPPPPPPPP 410
G PPPPPPPP
Sbjct: 298 GDSANGGLPPPPPPPP 313
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 307 PPPPPPPP 314
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 27.1 bits (57), Expect = 3.9
Identities = 10/25 (40%), Positives = 11/25 (44%)
Frame = +1
Query: 781 PPAXXTHPXPPPPXXXPPPXXHXPP 855
PP + P PPPP P PP
Sbjct: 354 PPQGRSAPPPPPPRSAPSTGRQPPP 378
Score = 26.6 bits (56), Expect = 5.2
Identities = 9/16 (56%), Positives = 10/16 (62%)
Frame = -3
Query: 457 GGGGXXXAPPPPPPPP 410
G G + PPPPPPP
Sbjct: 328 GNGSSNSSLPPPPPPP 343
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.1 bits (57), Expect = 3.9
Identities = 13/33 (39%), Positives = 13/33 (39%), Gaps = 2/33 (6%)
Frame = +1
Query: 769 PXTPPPAXXTHPXPPP--PXXXPPPXXHXPPXP 861
P P PA P PPP P P PP P
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVP 1211
Score = 25.8 bits (54), Expect = 9.1
Identities = 12/33 (36%), Positives = 13/33 (39%), Gaps = 2/33 (6%)
Frame = +1
Query: 769 PXTPPPAXXTHPXPPP--PXXXPPPXXHXPPXP 861
P P P+ P PPP P P PP P
Sbjct: 1198 PPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVP 1230
>SPBC13E7.03c |||RNA hairpin binding protein |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 713
Score = 27.1 bits (57), Expect = 3.9
Identities = 8/9 (88%), Positives = 9/9 (100%)
Frame = -3
Query: 436 APPPPPPPP 410
+PPPPPPPP
Sbjct: 369 SPPPPPPPP 377
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 27.1 bits (57), Expect = 3.9
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +1
Query: 775 TPPPAXXTHPXPPPPXXXP 831
T P THP PPPP P
Sbjct: 896 TSLPTIITHPTPPPPPPLP 914
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 945 PPPPPPPP 952
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 946 PPPPPPPP 953
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 189 PPPPPPPP 196
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 190 PPPPPPPP 197
>SPBC146.13c |myo1||myosin type I|Schizosaccharomyces pombe|chr
2|||Manual
Length = 1217
Score = 26.6 bits (56), Expect = 5.2
Identities = 8/8 (100%), Positives = 8/8 (100%)
Frame = -3
Query: 433 PPPPPPPP 410
PPPPPPPP
Sbjct: 1095 PPPPPPPP 1102
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 221 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 250
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 234 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 263
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 247 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 276
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 260 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 289
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 273 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 302
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 286 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 315
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 299 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 328
Score = 26.2 bits (55), Expect = 6.9
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 312 PPPPMHHEPGEHMPPPPMHHEPGEHMPPPP 341
Score = 25.8 bits (54), Expect = 9.1
Identities = 12/30 (40%), Positives = 12/30 (40%), Gaps = 2/30 (6%)
Frame = +1
Query: 778 PPPAXXTHPXP--PPPXXXPPPXXHXPPXP 861
PPP P PPP P H PP P
Sbjct: 208 PPPPMHHKPGEHMPPPPMHHEPGEHMPPPP 237
>SPBC83.01 |ucp8||UBA/EH/EF hand domain protein
Ucp8|Schizosaccharomyces pombe|chr 2|||Manual
Length = 884
Score = 25.8 bits (54), Expect = 9.1
Identities = 11/31 (35%), Positives = 11/31 (35%)
Frame = +1
Query: 769 PXTPPPAXXTHPXPPPPXXXPPPXXHXPPXP 861
P TP P PPPP PP P
Sbjct: 731 PPTPAPTPAVKHHPPPPPVRSSISPSMPPAP 761
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.316 0.152 0.532
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,366,050
Number of Sequences: 5004
Number of extensions: 17674
Number of successful extensions: 261
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 37
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 491307756
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.9 bits)
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