Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_M11
(903 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 313 3e-86
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 308 6e-85
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 161 1e-40
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 130 2e-31
SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting endonucl... 27 4.8
SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase Wis4|Schizo... 26 6.4
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.4
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 8.4
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 313 bits (768), Expect = 3e-86
Identities = 137/203 (67%), Positives = 166/203 (81%)
Frame = +2
Query: 152 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 331
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 332 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 511
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 512 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFAIYPAPQVSTA 691
+IR++AD C+GLQGFL+FH LL+ERL+++Y KSKL+F++YPAPQVST+
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 692 VVEPYNSILTTHTTLEHSDCAFM 760
VVEPYNS+LTTH TL+ +DC FM
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFM 203
Score = 51.6 bits (118), Expect = 1e-07
Identities = 22/28 (78%), Positives = 24/28 (85%)
Frame = +3
Query: 762 VDHEPIYDICRRNLDXERPTYTNLNRLI 845
VD+E YDICRRNLD ERP+Y NLNRLI
Sbjct: 204 VDNESCYDICRRNLDIERPSYENLNRLI 231
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 308 bits (757), Expect = 6e-85
Identities = 139/208 (66%), Positives = 169/208 (81%), Gaps = 5/208 (2%)
Frame = +2
Query: 152 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 316
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 317 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 496
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 497 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFAIYPAP 676
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YG KS L+F++YPAP
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 677 QVSTAVVEPYNSILTTHTTLEHSDCAFM 760
QVST+VVEPYNS+LTTH TL++SDC FM
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFM 207
Score = 53.2 bits (122), Expect = 5e-08
Identities = 23/28 (82%), Positives = 24/28 (85%)
Frame = +3
Query: 762 VDHEPIYDICRRNLDXERPTYTNLNRLI 845
VD+E YDICRRNLD ERPTY NLNRLI
Sbjct: 208 VDNEACYDICRRNLDIERPTYENLNRLI 235
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 161 bits (391), Expect = 1e-40
Identities = 79/202 (39%), Positives = 113/202 (55%)
Frame = +2
Query: 152 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 331
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 332 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 511
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 512 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFAIYPAPQVSTA 691
+R+ A+ C LQGF + H LL+ ++ +Y + F++ PAP+ S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 692 VVEPYNSILTTHTTLEHSDCAF 757
VVEPYN+ L+ H +E+SD F
Sbjct: 179 VVEPYNATLSMHQLVENSDETF 200
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 130 bits (315), Expect = 2e-31
Identities = 71/191 (37%), Positives = 109/191 (57%), Gaps = 3/191 (1%)
Frame = +2
Query: 155 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 334
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 335 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 508
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 509 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGXKSKLEFAIYPAPQ-VS 685
D I + AD L+GF + H L+ERL+ Y K ++++P Q VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 686 TAVVEPYNSIL 718
VV+PYNS+L
Sbjct: 180 DVVVQPYNSLL 190
>SPAC25G10.02 |cce1|ydc2|mitochondrial cruciform cutting
endonuclease Cce1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 258
Score = 26.6 bits (56), Expect = 4.8
Identities = 9/21 (42%), Positives = 13/21 (61%)
Frame = -1
Query: 834 SGWCRWGAQXRDYGGRCHRWV 772
SGW RW AQ + Y C +++
Sbjct: 236 SGWMRWQAQLKHYRNFCKQFL 256
>SPAC9G1.02 |wis4|wak1, wik1|MAP kinase kinase kinase
Wis4|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1401
Score = 26.2 bits (55), Expect = 6.4
Identities = 16/45 (35%), Positives = 24/45 (53%), Gaps = 1/45 (2%)
Frame = +2
Query: 35 NSLKILIHIRLASHLTGRAERRLI-II*FYI*A*KKTQIKMRECI 166
NSL + +H + SHL G ER + +Y K TQI+ R+ +
Sbjct: 604 NSLLLNVHRYVESHLNGPTERTAASLTNWYSTLLKNTQIRFRKIL 648
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 231 STASSLMARCPQTRPSGVETILSTLSSARPELAST 335
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 8.4
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 250 WPDAHRQDHRGWRRFFQHFLQR 315
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,442,648
Number of Sequences: 5004
Number of extensions: 70753
Number of successful extensions: 198
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 193
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 456499320
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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