Bombyx BLAST Result against uniref50

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP12_F_M02
         (936 letters)

Database: uniref50 
           1,657,284 sequences; 575,637,011 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5; Obtectomera|...   167   5e-40
UniRef50_P50725 Cluster: Attacin-A precursor; n=14; Obtectomera|...   145   2e-33
UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria cunea...   133   5e-30
UniRef50_Q95NH6 Cluster: Attacin-C precursor [Contains: Immune-i...    40   0.12 
UniRef50_Q5MGP9 Cluster: Defense protein 2; n=1; Lonomia obliqua...    36   1.1  
UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin ...    36   2.0  
UniRef50_Q17FI3 Cluster: Antibacterial peptide, putative; n=1; A...    35   2.6  
UniRef50_Q9FJS3 Cluster: Genomic DNA, chromosome 5, P1 clone:MJE...    35   3.4  
UniRef50_UPI000155657E Cluster: PREDICTED: similar to kleisin be...    34   6.0  
UniRef50_Q03052 Cluster: POU domain, class 3, transcription fact...    33   7.9  

>UniRef50_Q0Q042 Cluster: Attacin-like protein; n=5;
           Obtectomera|Rep: Attacin-like protein - Antheraea
           mylitta (Tasar silkworm)
          Length = 230

 Score =  167 bits (405), Expect = 5e-40
 Identities = 80/148 (54%), Positives = 98/148 (66%)
 Frame = +3

Query: 174 TRARRQAGSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRXKXXXXXXXXXXXXV 353
           +R RRQAG+ TVNSDGTSGAA+K+P+TGN+ + LSAIGS DFNDR K            V
Sbjct: 42  SRVRRQAGALTVNSDGTSGAAVKIPITGNENHKLSAIGSLDFNDRNKLGAATAGLAYDNV 101

Query: 354 NGHGLSLXGTRXPGFGEQLGXPGXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNFTTLGX 533
           NGHG +L  T  PGFG+++   G V L  N+NHDL+ANAF  RN P+  P  PNF T+G 
Sbjct: 102 NGHGATLTKTHIPGFGDKMTAAGKVNLFHNDNHDLNANAFATRNMPN-IPQVPNFNTVGG 160

Query: 534 GVDYMFXPKVGASLXPXHSDVXXRTDXS 617
           GVDYMF  ++GAS    H+D   R D S
Sbjct: 161 GVDYMFKDRIGASASAAHTDFINRNDYS 188


>UniRef50_P50725 Cluster: Attacin-A precursor; n=14;
           Obtectomera|Rep: Attacin-A precursor - Trichoplusia ni
           (Cabbage looper)
          Length = 254

 Score =  145 bits (351), Expect = 2e-33
 Identities = 74/148 (50%), Positives = 92/148 (62%), Gaps = 1/148 (0%)
 Frame = +3

Query: 177 RARRQA-GSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRXKXXXXXXXXXXXXV 353
           R RRQA GS T+NSDG+ G   KVP+ GN+KNVLSA+GS D ND+ K            V
Sbjct: 59  RVRRQAQGSVTLNSDGSMGLGAKVPIVGNEKNVLSALGSVDLNDQLKPASRGMGLALDNV 118

Query: 354 NGHGLSLXGTRXPGFGEQLGXPGXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNFTTLGX 533
           NGHGLS+     PGFG++L   G V +  N+NHD+SA AF  +N P   P+ PNF T+G 
Sbjct: 119 NGHGLSVMKETVPGFGDRLTGAGRVNVFHNDNHDISAKAFVTKNMPD-FPNVPNFNTVGG 177

Query: 534 GVDYMFXPKVGASLXPXHSDVXXRTDXS 617
           GVDYM+  KVGASL   ++    R D S
Sbjct: 178 GVDYMYKNKVGASLGMANTPFLDRKDYS 205


>UniRef50_O96361 Cluster: Putative attacin; n=1; Hyphantria
           cunea|Rep: Putative attacin - Hyphantria cunea (Fall
           webworm)
          Length = 233

 Score =  133 bits (322), Expect = 5e-30
 Identities = 72/147 (48%), Positives = 86/147 (58%)
 Frame = +3

Query: 177 RARRQAGSFTVNSDGTSGAALKVPLTGNDKNVLSAIGSADFNDRXKXXXXXXXXXXXXVN 356
           RARRQ GS  +N D TS A +K+PL G++KNVLSA+GS  F+                V 
Sbjct: 44  RARRQLGSVFLNPDSTSRANIKLPLAGSNKNVLSALGSVGFDANKHLSSASGGLALDNVR 103

Query: 357 GHGLSLXGTRXPGFGEQLGXPGXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNFTTLGXG 536
           GHGLSL GT  P FG QL   G + L  N NHDL+ANAF  RN P+  P  PNF T+G  
Sbjct: 104 GHGLSLTGTHIPNFGNQLTGAGRLNLFHNQNHDLNANAFLTRNMPT-IPQVPNFNTVG-S 161

Query: 537 VDYMFXPKVGASLXPXHSDVXXRTDXS 617
           ++YMF  KVGASL    +    RTD S
Sbjct: 162 LNYMFKNKVGASLGASRTPFLQRTDYS 188


>UniRef50_Q95NH6 Cluster: Attacin-C precursor [Contains:
           Immune-induced peptide 16 (DIM-16) (MPAC)]; n=21;
           Sophophora|Rep: Attacin-C precursor [Contains:
           Immune-induced peptide 16 (DIM-16) (MPAC)] - Drosophila
           melanogaster (Fruit fly)
          Length = 241

 Score = 39.5 bits (88), Expect = 0.12
 Identities = 40/139 (28%), Positives = 52/139 (37%), Gaps = 6/139 (4%)
 Frame = +3

Query: 177 RARRQA--GSFTVNSDGTSGAALKVP-LTGN-DKNVLSAIGSADFNDRXKXXXXXXXXXX 344
           RARRQ   GS T N  G + A L +    G  D +V+  + +A                 
Sbjct: 45  RARRQVLGGSLTSNPSGGADARLDLSKAVGTPDHHVIGQVFAAGNTQTKPVSTPVTSGAT 104

Query: 345 XXVN--GHGLSLXGTRXPGFGEQLGXPGXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNF 518
              N  GHGL L  T  PG  +         L  N  H+L A AF  +N      +   F
Sbjct: 105 LGYNNHGHGLELTKTHTPGVRDSFQQTATANLFNNGVHNLDAKAFASQNQ---LANGFKF 161

Query: 519 TTLGXGVDYMFXPKVGASL 575
              G  +DY      GA+L
Sbjct: 162 DRNGAALDYSHIKGHGATL 180


>UniRef50_Q5MGP9 Cluster: Defense protein 2; n=1; Lonomia
           obliqua|Rep: Defense protein 2 - Lonomia obliqua (Moth)
          Length = 113

 Score = 36.3 bits (80), Expect = 1.1
 Identities = 20/66 (30%), Positives = 32/66 (48%)
 Frame = +3

Query: 420 GXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNFTTLGXGVDYMFXPKVGASLXPXHSDVX 599
           G   +  N+NH+L      +  S S  P+  ++      +DY++  K+ ASL   HS + 
Sbjct: 4   GKYNILHNDNHNLDLTGKFLECSRS-NPNLSDYNKYSAILDYLYKDKLSASLGVAHSGLL 62

Query: 600 XRTDXS 617
            RTD S
Sbjct: 63  DRTDLS 68


>UniRef50_Q4PNY5 Cluster: Attacin; n=4; Calyptratae|Rep: Attacin -
           Musca domestica (House fly)
          Length = 208

 Score = 35.5 bits (78), Expect = 2.0
 Identities = 24/71 (33%), Positives = 31/71 (43%)
 Frame = +3

Query: 363 GLSLXGTRXPGFGEQLGXPGXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNFTTLGXGVD 542
           G SL  +R   FG          L  N+ H L ANAF  R +     +   F T+G G+D
Sbjct: 80  GGSLSHSRTDNFGSTFSQKLNANLFQNDKHKLDANAFHSRTN---LDNGFKFNTVGGGLD 136

Query: 543 YMFXPKVGASL 575
           Y      GAS+
Sbjct: 137 YNHANGHGASV 147


>UniRef50_Q17FI3 Cluster: Antibacterial peptide, putative; n=1;
           Aedes aegypti|Rep: Antibacterial peptide, putative -
           Aedes aegypti (Yellowfever mosquito)
          Length = 265

 Score = 35.1 bits (77), Expect = 2.6
 Identities = 39/138 (28%), Positives = 54/138 (39%), Gaps = 6/138 (4%)
 Frame = +3

Query: 177 RARRQAGSFTVNS----DGTSGAALKVPLTGNDKNV--LSAIGSADFNDRXKXXXXXXXX 338
           + R  AG F  +S    D T GA   + L    K+   +SA GS   N+  +        
Sbjct: 75  KGRNSAGIFGSHSLPGPDNTVGARGNLNLFSGQKDRFDVSAFGSQSTNNVKQFGTGLHF- 133

Query: 339 XXXXVNGHGLSLXGTRXPGFGEQLGXPGXVXLXXNNNHDLSANAFXIRNSPSAXPHAPNF 518
                N H  S   T  PG G Q    G   L    ++ L  NAF  R  P     +P+F
Sbjct: 134 -----NEHSFSATRTNQPGAGSQTRLDGSANLFKTPSNRLDLNAFKSRTQPVG---SPSF 185

Query: 519 TTLGXGVDYMFXPKVGAS 572
            + G G+++      GAS
Sbjct: 186 GSHGAGLNWNNANGHGAS 203


>UniRef50_Q9FJS3 Cluster: Genomic DNA, chromosome 5, P1 clone:MJE4;
           n=3; Arabidopsis thaliana|Rep: Genomic DNA, chromosome
           5, P1 clone:MJE4 - Arabidopsis thaliana (Mouse-ear
           cress)
          Length = 343

 Score = 34.7 bits (76), Expect = 3.4
 Identities = 17/42 (40%), Positives = 19/42 (45%)
 Frame = -2

Query: 740 GGXPGKGWCXXGGIXXLWEXGGGGGAXXGRXIGNRFXLRAGG 615
           GG  G GW   GG    W+ GGGGG   G   G  +    GG
Sbjct: 300 GGGHGGGWQGGGGRGGGWKGGGGGGGWRGGGGGGGWRGGGGG 341


>UniRef50_UPI000155657E Cluster: PREDICTED: similar to kleisin beta,
           partial; n=1; Ornithorhynchus anatinus|Rep: PREDICTED:
           similar to kleisin beta, partial - Ornithorhynchus
           anatinus
          Length = 478

 Score = 33.9 bits (74), Expect = 6.0
 Identities = 24/68 (35%), Positives = 30/68 (44%), Gaps = 3/68 (4%)
 Frame = -2

Query: 407 LLPEAGXAGPGQAQPVPVYVXQSEPCGGSAELXAVVEVGGPDGAEHVLVVAGERDL---Q 237
           LLP A   GPG    V       EPCG  A   AV  VGG +  +  +    + DL    
Sbjct: 284 LLPPAPLPGPGTGSGVGSVGVPLEPCGLLALAGAVEAVGGSEDEDGEVGGLPDEDLSVGD 343

Query: 236 GGPGGSVR 213
            GPGG ++
Sbjct: 344 PGPGGPLK 351


>UniRef50_Q03052 Cluster: POU domain, class 3, transcription factor
           1; n=10; Theria|Rep: POU domain, class 3, transcription
           factor 1 - Homo sapiens (Human)
          Length = 448

 Score = 33.5 bits (73), Expect = 7.9
 Identities = 23/74 (31%), Positives = 31/74 (41%), Gaps = 4/74 (5%)
 Frame = -2

Query: 401 PEAGXAGPGQAQPVPVYVXQSEPCGGSAELXAVVEVGG---PDGAEHVLVVAG-ERDLQG 234
           P  G +G  Q QP+ +Y   + P GG   L  ++  GG     G  H L   G E  L+ 
Sbjct: 140 PSPGASGGHQPQPLGLYAQAAYPGGGGGGLAGMLAAGGGGAGPGLHHALHEDGHEAQLEP 199

Query: 233 GPGGSVRVHCEAAG 192
            P   +  H  A G
Sbjct: 200 SPPPHLGAHGHAHG 213


  Database: uniref50
    Posted date:  Oct 5, 2007 11:19 AM
  Number of letters in database: 575,637,011
  Number of sequences in database:  1,657,284
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 550,546,207
Number of Sequences: 1657284
Number of extensions: 8743530
Number of successful extensions: 27845
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 22803
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 27404
length of database: 575,637,011
effective HSP length: 101
effective length of database: 408,251,327
effective search space used: 85732778670
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)