Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_L16
(896 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyc... 30 0.51
SPAC56F8.03 |||translation initiation factor IF2 |Schizosaccharo... 29 0.90
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.8
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 27 4.8
SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyc... 27 4.8
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 27 4.8
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 26 6.3
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.3
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 6.3
SPBC16E9.05 |erg6||delta-sterol C-methyltransferase |Schizosacch... 26 8.4
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.4
SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces pombe... 26 8.4
>SPAC1B3.05 |||CCR4-Not complex subunit Not3/5 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 630
Score = 29.9 bits (64), Expect = 0.51
Identities = 22/83 (26%), Positives = 43/83 (51%)
Frame = +3
Query: 288 QQGLEGRLRVRAATAQRLRQESPGXRSETRTARPREALEQSRQNIERTAEELRKAHPDVE 467
++ LEG L+ + QRLR + S + + ++AL ++R+ IE EE + +++
Sbjct: 30 KEKLEGDLKTQIKKLQRLRDQIKTWAS-SNDIKDKKALLENRRLIEAKMEEFKAVEREMK 88
Query: 468 KNATALREKLQAAVQNTVQESQK 536
A + +E L A + +E +K
Sbjct: 89 IKAFS-KEGLSIASKLDPKEKEK 110
>SPAC56F8.03 |||translation initiation factor IF2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1079
Score = 29.1 bits (62), Expect = 0.90
Identities = 25/100 (25%), Positives = 46/100 (46%), Gaps = 2/100 (2%)
Frame = +3
Query: 279 TGLQQGLEGRLRVRAATAQRLRQESPG-XRSETRTARPREALEQSRQNIERTAEELRKAH 455
T LQ+ LE + R R QR+R+E E R A EA ++ + ++ E +K
Sbjct: 248 TALQKMLEEK-RAREEEEQRIREEEARIAEEEKRLAEVEEARKEEARLKKKEKERKKKEE 306
Query: 456 PDVE-KNATALREKLQAAVQNTVQESQKLAKKVSSNVQGD 572
+ K + +++ QA Q +Q+ + +V+ G+
Sbjct: 307 MKAQGKYLSKKQKEQQALAQRRLQQMLESGVRVAGLSNGE 346
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.8
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +1
Query: 181 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 300
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 26.6 bits (56), Expect = 4.8
Identities = 10/30 (33%), Positives = 18/30 (60%)
Frame = +1
Query: 175 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT 264
++ DFFK + ++ H TL ++ NSL+
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLS 85
>SPAC4G9.19 |||DNAJ domain protein DNAJB family|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 26.6 bits (56), Expect = 4.8
Identities = 19/45 (42%), Positives = 25/45 (55%), Gaps = 5/45 (11%)
Frame = +3
Query: 366 SETRTARPREALEQSR----QNIERTAEEL-RKAHPDVEKNATAL 485
SE ++ P E LE R +I+R EL +K HPD KNA+ L
Sbjct: 26 SELKSLTPYEILELPRTCTANDIKRKYIELVKKHHPDKMKNASQL 70
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 26.6 bits (56), Expect = 4.8
Identities = 16/67 (23%), Positives = 31/67 (46%)
Frame = +3
Query: 339 LRQESPGXRSETRTARPREALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNT 518
LRQ S ++ + + E E +QN E+ + E+ + +EKL A ++
Sbjct: 46 LRQLSEINEAQKKAEKSLEQTEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRK 105
Query: 519 VQESQKL 539
+ S++L
Sbjct: 106 QKLSEEL 112
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 26.2 bits (55), Expect = 6.3
Identities = 19/64 (29%), Positives = 29/64 (45%)
Frame = +1
Query: 88 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 267
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 268 SKDA 279
SKDA
Sbjct: 230 SKDA 233
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.3
Identities = 14/50 (28%), Positives = 23/50 (46%)
Frame = +1
Query: 217 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGSA 366
+K+ + E+Q +T DFS K + L+AF K L+ S+
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASS 138
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +3
Query: 399 LEQSRQNIERTAEELRKAHPDVEK 470
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC16E9.05 |erg6||delta-sterol C-methyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 8.4
Identities = 17/49 (34%), Positives = 23/49 (46%), Gaps = 2/49 (4%)
Frame = -2
Query: 568 PCTFEDTFFANFWDSCTVFCTAACS--FSRRAVAFFSTSGWALRSSSAV 428
P T + T N WD TVF T+ +R +V F G A + +S V
Sbjct: 295 PLTGDITKCQNIWDVFTVFRTSRLGKLVTRYSVQFLEKIGVAAKGTSKV 343
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.4
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +1
Query: 145 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 321
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 322 LQQL 333
Q+
Sbjct: 73 NNQI 76
>SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 229
Score = 25.8 bits (54), Expect = 8.4
Identities = 19/67 (28%), Positives = 33/67 (49%)
Frame = +3
Query: 369 ETRTARPREALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKK 548
E + R +E E S++ E E+ RKA D +N R+K+ + + +E +KL ++
Sbjct: 114 EDQMKRIQERDESSKKLRESNIEKARKAIDDFYENFNDKRDKV---IAKSRKEQEKLLEE 170
Query: 549 VSSNVQG 569
S G
Sbjct: 171 NESKSTG 177
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,305,641
Number of Sequences: 5004
Number of extensions: 35874
Number of successful extensions: 170
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 164
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 170
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 452494940
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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