Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_K10
(886 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC637.08 |||iron-sulfur cluster assembly ATPase Nbp35|Schizosa... 29 1.2
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 28 1.5
SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase ... 27 3.6
SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7 domain|Schizosaccharo... 27 3.6
SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity f... 27 4.7
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 26 6.2
SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor ... 26 8.2
SPBC1921.06c |pvg3|mug49|beta-1,3-galactosyltransferase |Schizos... 26 8.2
SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces pombe... 26 8.2
>SPAC637.08 |||iron-sulfur cluster assembly ATPase
Nbp35|Schizosaccharomyces pombe|chr 1|||Manual
Length = 317
Score = 28.7 bits (61), Expect = 1.2
Identities = 24/103 (23%), Positives = 41/103 (39%), Gaps = 1/103 (0%)
Frame = +1
Query: 172 CPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTVEYHDVKTRGFDT-I 348
CPN + I + RGP + L + + D+ NL D D +
Sbjct: 129 CPNLAVMSIGFLLPSEDSSVIWRGPKKNGLIKQFIK-DVNWENLDYLIVDTPPGTSDEHL 187
Query: 349 KIIEFYINSKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIV 477
+++F+ NS + V+ Q + L + + RKA PI+
Sbjct: 188 SLVQFFKNSGIDGAVVVTTPQEVALQDVRKEIDFCRKASIPIL 230
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/34 (41%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +1
Query: 160 IKRPCPN-FDLNCIREYFSRNSQCQLVRGPVPDP 258
I+ PC + F NCI+ + N C + R PV DP
Sbjct: 410 IQLPCKHYFHENCIKPWLRVNGTCAICRAPV-DP 442
>SPBC26H8.03 |cho2||phosphatidylethanolamine N-methyltransferase
Cho2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 905
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/22 (50%), Positives = 14/22 (63%)
Frame = -1
Query: 514 LEFHSQLPMRHFLQLALWLSYC 449
LEF+S L RHF+ L L +C
Sbjct: 199 LEFNSWLVFRHFVDLILMCDFC 220
>SPAC4D7.01c |sec71|sec7a, SPAP8A3.15c|Sec7
domain|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1811
Score = 27.1 bits (57), Expect = 3.6
Identities = 25/90 (27%), Positives = 43/90 (47%), Gaps = 6/90 (6%)
Frame = +1
Query: 352 IIEFYIN-----SKTEKLVLAAEVQSLKLASPKTIFKYNRKAKEPIVRSDALEVDYGTLT 516
+IE Y+N TE + A V ++AS T + + R D L +D
Sbjct: 527 LIELYLNYDCISGNTENIYERAIVTLSRIASQST----SDPPPSFVFRDDQLVIDKPGF- 581
Query: 517 FTAVFPSISDL-QLSNAEVFSYVHEINPKF 603
V+ +++D+ QL+++ + SYVH NP +
Sbjct: 582 ---VYHTLNDIPQLNSSTIGSYVHSHNPPY 608
>SPAC22H10.05c |||mRNA cleavage and polyadenylation specificity
factor complex subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 456
Score = 26.6 bits (56), Expect = 4.7
Identities = 14/37 (37%), Positives = 21/37 (56%)
Frame = +3
Query: 90 VLGLIASAVAFGXNLDPADDPKKHQKTLSELRLELHS 200
VL + FG + P D+PK ++ +LS L L +HS
Sbjct: 183 VLAKVPLVYNFGLD-SPLDNPKLYKLSLSRLALAVHS 218
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 26.2 bits (55), Expect = 6.2
Identities = 11/31 (35%), Positives = 17/31 (54%), Gaps = 1/31 (3%)
Frame = +1
Query: 163 KRPCPN-FDLNCIREYFSRNSQCQLVRGPVP 252
K PC + F NC++++ + C L R VP
Sbjct: 124 KMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154
>SPCC1442.01 |ste6|SPCC1450.17|guanyl-nucleotide exchange factor
Ste6|Schizosaccharomyces pombe|chr 3|||Manual
Length = 911
Score = 25.8 bits (54), Expect = 8.2
Identities = 20/82 (24%), Positives = 34/82 (41%)
Frame = +1
Query: 130 TWIRPMTQKNIKRPCPNFDLNCIREYFSRNSQCQLVRGPVPDPLPLSYYRVDIPNSNLTV 309
T I+P K FDLN + S+ C L++ P+PL + + N +
Sbjct: 428 TNIQPELNDAEKSQLDGFDLNKTNWFDSKGLVCYLMKQTSPEPLLIRNLLFSFWSCNGKI 487
Query: 310 EYHDVKTRGFDTIKIIEFYINS 375
E D K + + II + + +
Sbjct: 488 E-QDGKIKTATLVFIINYLLRT 508
>SPBC1921.06c |pvg3|mug49|beta-1,3-galactosyltransferase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 25.8 bits (54), Expect = 8.2
Identities = 14/49 (28%), Positives = 25/49 (51%), Gaps = 4/49 (8%)
Frame = -1
Query: 301 NYYSVYLLCSNLKVVDLVQVPLLIDIENFLRNIHE----CSSSRSSDRV 167
NY YL + V L +PLL + N+ + ++E CS+ ++R+
Sbjct: 316 NYRQSYLRIDAIAVHKLKSIPLLSTVSNWFKKMYEHRANCSALIETERL 364
>SPAC3A11.08 |pcu4|cul4, Cul-4|cullin 4|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 734
Score = 25.8 bits (54), Expect = 8.2
Identities = 15/53 (28%), Positives = 24/53 (45%)
Frame = -1
Query: 322 HHDILRLNYYSVYLLCSNLKVVDLVQVPLLIDIENFLRNIHECSSSRSSDRVF 164
H D + S+ L L V +Q+P L + N+H SS+S+D +
Sbjct: 136 HPDYPTIEELSLSLFREKLMAVKNIQIPFLNSLLQSFENLH---SSKSTDHAY 185
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,856,281
Number of Sequences: 5004
Number of extensions: 53466
Number of successful extensions: 154
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 147
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 154
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 444486180
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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