Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_J22
(858 letters)
Database: bee
438 sequences; 146,343 total letters
Searching......................................................done
Score E
Sequences producing significant alignments: (bits) Value
AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1al... 383 e-108
X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alp... 378 e-107
AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor 1-a... 264 5e-73
EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor 1-a... 231 5e-63
AF069739-1|AAC63272.2| 690|Apis mellifera translation initiatio... 33 0.003
AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9 methylt... 24 1.6
DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450 monoo... 23 3.6
AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase pro... 23 3.6
D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein. 23 4.8
>AF015267-1|AAC38959.1| 461|Apis mellifera elongation factor-1alpha
F2 protein.
Length = 461
Score = 383 bits (942), Expect = e-108
Identities = 186/205 (90%), Positives = 186/205 (90%)
Frame = +2
Query: 110 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 289
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 290 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 469
DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT
Sbjct: 61 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 120
Query: 470 GEFEAXISKNGQTREHALLAFTLXVKQLIVXVXKMDSTEPPYSEPXFEXIXKXVSSYIKX 649
GEFEA ISKNGQTREHALLAFTL VKQLIV V KMDSTEPPYSE FE I K VSSYIK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKK 180
Query: 650 IGYXPXAVAFVPISGWXGXXXLXPS 724
IGY P AVAFVPISGW G L S
Sbjct: 181 IGYNPAAVAFVPISGWHGDNMLEVS 205
Score = 24.2 bits (50), Expect = 1.6
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = +1
Query: 730 MPWFXGWXVXPK 765
MPWF GW V K
Sbjct: 208 MPWFKGWTVERK 219
>X52884-1|CAA37066.1| 461|Apis mellifera elongation factor 1 alpha
protein.
Length = 461
Score = 378 bits (930), Expect = e-107
Identities = 182/206 (88%), Positives = 185/206 (89%)
Frame = +2
Query: 110 MGKEKTHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 289
MGKEK HINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL
Sbjct: 1 MGKEKIHINIVVIGHVDSGKSTTTGHLIYKCGGIDKRTIEKFEKEAQEMGKGSFKYAWVL 60
Query: 290 DKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGT 469
DKLKAERERGITIDIALWKFET+KYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAG
Sbjct: 61 DKLKAERERGITIDIALWKFETAKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGI 120
Query: 470 GEFEAXISKNGQTREHALLAFTLXVKQLIVXVXKMDSTEPPYSEPXFEXIXKXVSSYIKX 649
GEFEA ISKNGQTREHALLAFTL VKQLIV V KMD T+PPYSE FE I K VSSYIK
Sbjct: 121 GEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDMTDPPYSEARFEEIKKEVSSYIKK 180
Query: 650 IGYXPXAVAFVPISGWXGXXXLXPSP 727
IGY +VAFVPISGW G L PSP
Sbjct: 181 IGYNTASVAFVPISGWHGDNMLEPSP 206
>AY208278-1|AAO48970.1| 274|Apis mellifera elongation factor
1-alpha protein.
Length = 274
Score = 264 bits (648), Expect = 5e-73
Identities = 130/148 (87%), Positives = 130/148 (87%)
Frame = +2
Query: 281 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 460
WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA
Sbjct: 1 WVLDKLKAERERGITIDIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVA 60
Query: 461 AGTGEFEAXISKNGQTREHALLAFTLXVKQLIVXVXKMDSTEPPYSEPXFEXIXKXVSSY 640
AGTGEFEA ISKNGQTREHALLAFTL VKQLIV V KMDSTEPPYSE FE I K VSSY
Sbjct: 61 AGTGEFEAGISKNGQTREHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSY 120
Query: 641 IKXIGYXPXAVAFVPISGWXGXXXLXPS 724
IK IGY P AVAFVPISGW G L S
Sbjct: 121 IKKIGYNPAAVAFVPISGWHGDNMLEVS 148
Score = 24.2 bits (50), Expect = 1.6
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = +1
Query: 730 MPWFXGWXVXPK 765
MPWF GW V K
Sbjct: 151 MPWFKGWTVERK 162
>EF013389-1|ABK54743.1| 172|Apis mellifera elongation factor
1-alpha protein.
Length = 172
Score = 231 bits (566), Expect = 5e-63
Identities = 114/132 (86%), Positives = 114/132 (86%)
Frame = +2
Query: 329 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAXISKNGQT 508
DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEA ISKNGQT
Sbjct: 1 DIALWKFETSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTGEFEAGISKNGQT 60
Query: 509 REHALLAFTLXVKQLIVXVXKMDSTEPPYSEPXFEXIXKXVSSYIKXIGYXPXAVAFVPI 688
REHALLAFTL VKQLIV V KMDSTEPPYSE FE I K VSSYIK IGY P AVAFVPI
Sbjct: 61 REHALLAFTLGVKQLIVGVNKMDSTEPPYSETRFEEIKKEVSSYIKKIGYNPAAVAFVPI 120
Query: 689 SGWXGXXXLXPS 724
SGW G L S
Sbjct: 121 SGWHGDNMLEVS 132
Score = 24.2 bits (50), Expect = 1.6
Identities = 8/12 (66%), Positives = 8/12 (66%)
Frame = +1
Query: 730 MPWFXGWXVXPK 765
MPWF GW V K
Sbjct: 135 MPWFKGWTVERK 146
>AF069739-1|AAC63272.2| 690|Apis mellifera translation initiation
factor 2 protein.
Length = 690
Score = 33.1 bits (72), Expect = 0.003
Identities = 16/35 (45%), Positives = 18/35 (51%)
Frame = +2
Query: 368 VTIIDAPGHRDFIKNMITGTSQADCAVLIVAAGTG 472
VT +D PGH FI G D VL+VAA G
Sbjct: 195 VTFLDTPGHAAFISMRHRGAHITDIVVLVVAADDG 229
Score = 25.4 bits (53), Expect = 0.68
Identities = 9/18 (50%), Positives = 13/18 (72%)
Frame = +2
Query: 122 KTHINIVVIGHVDSGKST 175
K H + ++GHVD GK+T
Sbjct: 143 KRHPIVTIMGHVDHGKTT 160
>AM050259-1|CAJ18340.1| 683|Apis mellifera putative H3K9
methyltransferase protein.
Length = 683
Score = 24.2 bits (50), Expect = 1.6
Identities = 11/15 (73%), Positives = 11/15 (73%)
Frame = +2
Query: 131 INIVVIGHVDSGKST 175
INI IGHV GKST
Sbjct: 43 INIGTIGHVAHGKST 57
>DQ244074-1|ABB36784.1| 517|Apis mellifera cytochrome P450
monooxygenase protein.
Length = 517
Score = 23.0 bits (47), Expect = 3.6
Identities = 10/17 (58%), Positives = 12/17 (70%)
Frame = -1
Query: 123 FSLPIFG*SRITNCV*Y 73
FSLPIFG I +C+ Y
Sbjct: 57 FSLPIFGTRWIFSCIGY 73
>AB253417-1|BAE86928.1| 567|Apis mellifera alpha-glucosidase
protein.
Length = 567
Score = 23.0 bits (47), Expect = 3.6
Identities = 13/34 (38%), Positives = 18/34 (52%)
Frame = +2
Query: 353 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 454
T KYY D P + FIKN+ ++ +D LI
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLI 327
>D79208-1|BAA11466.1| 567|Apis mellifera alpha-glucosidase protein.
Length = 567
Score = 22.6 bits (46), Expect = 4.8
Identities = 12/34 (35%), Positives = 18/34 (52%)
Frame = +2
Query: 353 TSKYYVTIIDAPGHRDFIKNMITGTSQADCAVLI 454
T KYY D P + FIKN+ ++ +D L+
Sbjct: 294 TMKYYDYGADFPFNFAFIKNVSRDSNSSDFKKLV 327
Database: bee
Posted date: Oct 23, 2007 1:17 PM
Number of letters in database: 146,343
Number of sequences in database: 438
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 187,861
Number of Sequences: 438
Number of extensions: 3348
Number of successful extensions: 18
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 13
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 18
length of database: 146,343
effective HSP length: 57
effective length of database: 121,377
effective search space used: 27673956
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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