BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_G18
(1068 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 33 0.069
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 30 0.64
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 29 1.5
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 5.9
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 33.1 bits (72), Expect = 0.069
Identities = 21/81 (25%), Positives = 28/81 (34%)
Frame = +2
Query: 473 ALLSPQXRXXPXRPXXXCQPPXAXXRSXXRLPSXLXARXPXXXXXAPXXPXSPPXPXXPS 652
A +P P P +PP + +P A P AP P S P P P+
Sbjct: 120 ASAAPPSAPAPPTPQSELRPPTSAP-PRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPA 178
Query: 653 SPPDXXXHSSX*PLLTPXXPP 715
+P + P P PP
Sbjct: 179 APVKSPPSAPSLPSAVPPMPP 199
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.9 bits (64), Expect = 0.64
Identities = 24/86 (27%), Positives = 27/86 (31%), Gaps = 2/86 (2%)
Frame = +2
Query: 530 PPXAXXRSXXRLPSXLXARXPXXXXXA--PXXPXSPPXPXXPSSPPDXXXHSSX*PLLTP 703
PP RS LP A P P S P PS+PP + P L P
Sbjct: 392 PPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPP 451
Query: 704 XXPPAXRLXXXRXXTPXQXXXSPPXP 781
P A L P +P P
Sbjct: 452 SAPIAPPLPAGMPAAPPLPPAAPAPP 477
Score = 28.7 bits (61), Expect = 1.5
Identities = 18/65 (27%), Positives = 22/65 (33%), Gaps = 1/65 (1%)
Frame = +2
Query: 470 PALLSPQXRXXPXRPXXXCQPPXAXXRSXXRLPSXLXARXPXXXXXAPXXPXSPPXP-XX 646
P L + P P PP A P L P P P +PP P
Sbjct: 404 PPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGM 463
Query: 647 PSSPP 661
P++PP
Sbjct: 464 PAAPP 468
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 28.7 bits (61), Expect = 1.5
Identities = 21/68 (30%), Positives = 24/68 (35%)
Frame = -2
Query: 821 GXRGXQXXWVGRSXXXXXXXXXGXGXXXGXX*AVXRGGCXG*GGVXGXSGXXGLEGXRGG 642
G RG G G G G RGG G GG G G G G RGG
Sbjct: 9 GGRGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARG--GRGGSSGGRGG 66
Query: 641 XEXGXXLV 618
+ G ++
Sbjct: 67 AKGGAKVI 74
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.6 bits (56), Expect = 5.9
Identities = 12/32 (37%), Positives = 14/32 (43%)
Frame = +3
Query: 747 PLPXXTXPPPXRXPAYPXXLXSPXPPSPPSXS 842
P P + PPP P P S PP P+ S
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASS 1739
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,310,608
Number of Sequences: 5004
Number of extensions: 33144
Number of successful extensions: 122
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 118
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 563206452
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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