BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_F05
(1253 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 34 0.036
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 33 0.11
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.11
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 9.5
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.3 bits (75), Expect = 0.036
Identities = 25/104 (24%), Positives = 28/104 (26%), Gaps = 1/104 (0%)
Frame = +2
Query: 740 AXPXXXPHAPXXXSHPXXXSTXXPPPXSS-PLHXXTRXXQPHXPXPSXXXXXXXXXXXIS 916
A P P P + P P S P P P PS +
Sbjct: 1149 APPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVP 1208
Query: 917 XXXXPXPRPPRXXPXPXQPPXXXPFARLPXXPVXRPSGPXXSXP 1048
P PP P PP P A+ P P P S P
Sbjct: 1209 PVPPPSTAPPVPTPSAGLPPVPVPTAKAPPVPAPSSEAPSVSTP 1252
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||Manual
Length = 309
Score = 32.7 bits (71), Expect = 0.11
Identities = 25/99 (25%), Positives = 29/99 (29%)
Frame = +1
Query: 883 PXSHHXSPNHLXXXRPXPPXXPXPXXSXXAPXPXXLRSPPXXSRXPSFXAXXLPTRXXHS 1062
P H + P P P P P +PP S P A P
Sbjct: 110 PKLRHIGKSSASAAPPSAPAPPTPQSELRPPTS----APPRPSIPPPSPASAPPIPSKAP 165
Query: 1063 APXXNLPSPXSXXSXXPXXPSEPSPPX*TXXXPPXXPXP 1179
+LP P + PS PS P PP P P
Sbjct: 166 PIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPP 204
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.7 bits (71), Expect = 0.11
Identities = 40/171 (23%), Positives = 50/171 (29%), Gaps = 13/171 (7%)
Frame = +1
Query: 700 PPXXXQSNAVPLTSXPXXXXARXXPXQPPXXXLHXRPXTXQLAXPXXHASLPTAXPSXLH 879
PP P++ P P PP + P P PS +
Sbjct: 267 PPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPP-----PPPPPSRRN 321
Query: 880 X--PXSHHXSPNHLXXXRPXPPXX------PXPXXSXXAPXPXXLRSPPXXSRXP----S 1023
P + S N P PP P P AP P RS P R P S
Sbjct: 322 RGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGRQPPPLSS 381
Query: 1024 FXAXXLPTRXXHSAPXXNLPS-PXSXXSXXPXXPSEPSPPX*TXXXPPXXP 1173
A P + P + P+ P + P P+PP PP P
Sbjct: 382 SRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLP 432
Score = 30.3 bits (65), Expect = 0.58
Identities = 39/150 (26%), Positives = 48/150 (32%), Gaps = 3/150 (2%)
Frame = +1
Query: 700 PPXXXQSNAVPLTSXPXXXXARXXPXQPPXXXLHXRPXTXQLAXPXXHA---SLPTAXPS 870
PP +SNA S P R P PP P T + P + S P A P
Sbjct: 339 PPPPPRSNAAG--SIPLPPQGRSAPPPPPP---RSAPSTGRQPPPLSSSRAVSNPPAPPP 393
Query: 871 XLHXPXSHHXSPNHLXXXRPXPPXXPXPXXSXXAPXPXXLRSPPXXSRXPSFXAXXLPTR 1050
+ + P PP P AP +PP A LP
Sbjct: 394 AIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAP-AAPPLPP- 451
Query: 1051 XXHSAPXXNLPSPXSXXSXXPXXPSEPSPP 1140
SAP P P + P P+ P+PP
Sbjct: 452 ---SAPIAP-PLPAGMPAAPPLPPAAPAPP 477
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 9.5
Identities = 13/49 (26%), Positives = 18/49 (36%)
Frame = +3
Query: 732 PHEXTPXXXRTXXPXXATXPXPPRPXXHLPARXSXPPRVXXNRTTLXPP 878
P P ++ P + P PP P +P S PP + PP
Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,847,974
Number of Sequences: 5004
Number of extensions: 17517
Number of successful extensions: 68
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 22
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 57
length of database: 2,362,478
effective HSP length: 75
effective length of database: 1,987,178
effective search space used: 679614876
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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