Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_D08
(853 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr 2|||M... 106 3e-24
SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha sub... 36 0.010
SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr 2... 35 0.013
SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces pomb... 27 2.6
SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|c... 27 3.4
SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|c... 27 4.5
SPBC1604.06c |||CBF/Mak21 family|Schizosaccharomyces pombe|chr 2... 26 7.8
SPBC14C8.15 |||triglyceride lipase-cholesterol esterase |Schizos... 26 7.8
>SPBC2G5.05 |||transketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 685
Score = 106 bits (255), Expect = 3e-24
Identities = 69/205 (33%), Positives = 102/205 (49%), Gaps = 14/205 (6%)
Frame = +2
Query: 164 NKLRIDSIVATNASKSGHPTSCASMAEIMSVLFFHTMRYKISAPRDASADRFILSKGHAA 343
N +R ++ T +KSGHP + +A VLF M++ + P+ + DRFILS GHA
Sbjct: 15 NTIRTLAVDTTAHAKSGHPGAPMGLAPAAHVLFSRIMKFNPAHPKWLNRDRFILSNGHAC 74
Query: 344 PILYAAWAEAGL-FPLDELKNLRKLDSDLEGHP---TPRLNFVDVGTGSLGQGLAVAAGM 511
+ Y G +++LK R++ S GHP P LN ++ G G LGQG+A A G+
Sbjct: 75 VLQYIMCHLLGYKLTIEDLKQFRQVGSKTPGHPETHNPDLN-IETGAGPLGQGIASAVGL 133
Query: 512 AYVGKYFDQAPYR----------VYCLVGDGXAAXGSIXESLHFASHYKLDNLVVIFDVN 661
A +GK A Y +C +GDG G E+ A H KL NL+ ++D N
Sbjct: 134 A-IGKAHSAAVYNKPGFDLFSNYTFCFLGDGCLQEGVSSEACSLAGHLKLSNLIAVWDNN 192
Query: 662 RLGQSXPTSLQHXLEXYDXRLXAFG 736
++ TS+ E + R A+G
Sbjct: 193 KITIDGATSMSFD-EDVEKRFEAYG 216
>SPAC26F1.03 |pda1||pyruvate dehydrogenase e1 component alpha
subunit Pda1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 409
Score = 35.5 bits (78), Expect = 0.010
Identities = 21/62 (33%), Positives = 32/62 (51%)
Frame = +2
Query: 467 GTGSLGQGLAVAAGMAYVGKYFDQAPYRVYCLVGDGXAAXGSIXESLHFASHYKLDNLVV 646
G G +G + + AG+ + KY ++ P + L GDG + G E+ + A KL L V
Sbjct: 180 GNGIVGAQIPLGAGIGFAQKYLEK-PTTTFALYGDGASNQGQAFEAFNMA---KLWGLPV 235
Query: 647 IF 652
IF
Sbjct: 236 IF 237
>SPBC24C6.09c |||phosphoketolase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 825
Score = 35.1 bits (77), Expect = 0.013
Identities = 38/115 (33%), Positives = 49/115 (42%), Gaps = 9/115 (7%)
Frame = +2
Query: 317 FILSKGHAAP-ILYAAWAEAGLFPL--------DELKNLRKLDSDLEGHPTPRLNFVDVG 469
F++ GH AP IL A + E L P + L NL S G P+ +N G
Sbjct: 125 FVVGPGHGAPAILSALFLEDSLGPFYPRYQFTKEGLNNLINTFSLPGGFPS-HVNAEVPG 183
Query: 470 TGSLGQGLAVAAGMAYVGKYFDQAPYRVYCLVGDGXAAXGSIXESLHFASHYKLD 634
G L A ++Y G D+ V C+VGDG A G S H +H LD
Sbjct: 184 AIHEGGELGYALSVSY-GAVLDRPDLIVTCVVGDGEAETGPTATSWH--AHKFLD 235
>SPBC725.04 |||oxalyl-CoA decarboxylase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 574
Score = 27.5 bits (58), Expect = 2.6
Identities = 26/100 (26%), Positives = 44/100 (44%)
Frame = +2
Query: 362 WAEAGLFPLDELKNLRKLDSDLEGHPTPRLNFVDVGTGSLGQGLAVAAGMAYVGKYFDQA 541
W G +D + L ++ HP RL+ + T +G G A+A+ A+ D+
Sbjct: 390 WVSEGANTMDRGRQLLEVT-----HPRGRLDAGTMSTMGVGMGYAIASAFAHSS---DK- 440
Query: 542 PYRVYCLVGDGXAAXGSIXESLHFASHYKLDNLVVIFDVN 661
+V +G +A G L A +LD LV++ + N
Sbjct: 441 -----IVVVEGDSAFGFSAMELETAIRNQLDLLVIVINNN 475
>SPAC8C9.09c |mug129||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 302
Score = 27.1 bits (57), Expect = 3.4
Identities = 13/25 (52%), Positives = 16/25 (64%), Gaps = 1/25 (4%)
Frame = -2
Query: 540 AWSKYFPTYAMPAATASPWP-REPV 469
AWS + TY AAT SP P ++PV
Sbjct: 181 AWSLFTSTYFQKAATVSPSPNQQPV 205
>SPAC26A3.15c |nsp1||nucleoporin Nsp1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 598
Score = 26.6 bits (56), Expect = 4.5
Identities = 12/20 (60%), Positives = 13/20 (65%)
Frame = -2
Query: 513 AMPAATASPWPREPVPTSTK 454
A P ATAS P EP P+S K
Sbjct: 401 AKPGATASTTPSEPPPSSIK 420
>SPBC1604.06c |||CBF/Mak21 family|Schizosaccharomyces pombe|chr
2|||Manual
Length = 485
Score = 25.8 bits (54), Expect = 7.8
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = -2
Query: 510 MPAATASPWPREPVPTSTKLS 448
+P+++ S W EP P+ST+ S
Sbjct: 204 LPSSSTSSWADEPSPSSTETS 224
>SPBC14C8.15 |||triglyceride lipase-cholesterol esterase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 460
Score = 25.8 bits (54), Expect = 7.8
Identities = 9/24 (37%), Positives = 15/24 (62%)
Frame = -2
Query: 105 YSMPYLFCHETTLNASAWIKSLKE 34
Y P LF H +N+ +W+ +LK+
Sbjct: 117 YLPPVLFIHGLMMNSESWVCNLKK 140
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,588,387
Number of Sequences: 5004
Number of extensions: 48295
Number of successful extensions: 136
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 130
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 133
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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