Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_D04
(901 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC139.05 |||succinate-semialdehyde dehydrogenase |Schizosaccha... 76 6e-15
SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces pombe... 67 4e-12
SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|... 59 1e-09
SPCC550.10 |meu8||betaine aldehyde dehydrogenase |Schizosaccharo... 53 5e-08
SPAC1002.12c |||succinate-semialdehyde dehydrogenase |Schizosacc... 39 8e-04
SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces pomb... 38 0.001
SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces po... 32 0.13
SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7 |Schizos... 31 0.29
SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate dehydrogenase|Sch... 31 0.29
SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyc... 27 3.6
>SPAC139.05 |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 493
Score = 76.2 bits (179), Expect = 6e-15
Identities = 34/96 (35%), Positives = 58/96 (60%)
Frame = +1
Query: 220 YIDGQYVDSRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQ 399
++ G+++ S E+ NPAT E+IG+V + + +E A+ AA A+KT+ T + R
Sbjct: 21 FVQGKWISSPNNKTFEVDNPATGEIIGKVADVSVEETKKAISAANEAFKTYKNFTHVQRS 80
Query: 400 QLMFKFARLLRENQSKLAAKITEEQGKTIADAEGDV 507
QL+ ++A L+ EN+ L +T E GK ++ AE +V
Sbjct: 81 QLLERWAELIMENKDDLVKMLTLENGKPLSQAEMEV 116
Score = 34.3 bits (75), Expect = 0.024
Identities = 20/69 (28%), Positives = 32/69 (46%)
Frame = +2
Query: 602 IATRCHLGVVGGVAXFNFPVMIPLWMFPPALVTGNXCIIXPSXQDPXXTLMMMELLXEXX 781
I+ + +GV + +NFP + AL G I P+ + P L ++ L E
Sbjct: 149 ISIKQPVGVSALITPWNFPAAMIARKGGAALAAGCTAIFLPAFRTPYVCLGLVRLAQEAG 208
Query: 782 APPGVVNII 808
P GV+N+I
Sbjct: 209 FPDGVLNVI 217
>SPAC922.07c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 496
Score = 66.9 bits (156), Expect = 4e-12
Identities = 44/148 (29%), Positives = 74/148 (50%), Gaps = 1/148 (0%)
Frame = +1
Query: 217 LYIDGQYVDSRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTR 396
LYI+G++ S T W E +P+ EVI +V A + E+ A+ +AK A+KTW K +
Sbjct: 23 LYINGEWHKSAET-W-ETVDPSIEEVIAKVYLAGEKEIDYAVKSAKEAFKTWKKVPGSEK 80
Query: 397 QQLMFKFARLLRENQSKLAAKITEEQGK-TIADAEGDVLRGIQSVEHCCSITSLQLGDSI 573
+L+ K A L ++ LAA + GK +++A GDV I + +C G I
Sbjct: 81 GELLMKLAELTEKHADTLAAIEAMDSGKPLVSNARGDVDGTIALLRYCAGWADKIYGQVI 140
Query: 574 QNIAKDMDTHSYKVPLGSCWRCCXLQFP 657
+ + ++ + P+G C + +P
Sbjct: 141 PTGPEKL-AYAKRTPIGVCGQIVPWNYP 167
Score = 53.2 bits (122), Expect = 5e-08
Identities = 29/78 (37%), Positives = 37/78 (47%)
Frame = +2
Query: 605 ATRCHLGVVGGVAXFNFPVMIPLWMFPPALVTGNXCIIXPSXQDPXXTLMMMELLXEXXA 784
A R +GV G + +N+P+ + W PAL GN II + P L L+ E
Sbjct: 150 AKRTPIGVCGQIVPWNYPLNMAGWKIAPALAAGNCIIIKSAETTPLSLLYFATLVEEAGF 209
Query: 785 PPGVVNIIPGTTXRXTSY 838
P GVVNII G SY
Sbjct: 210 PKGVVNIISGLGTVAGSY 227
>SPAC9E9.09c |||aldehyde dehydrogenase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 503
Score = 58.8 bits (136), Expect = 1e-09
Identities = 38/150 (25%), Positives = 73/150 (48%), Gaps = 3/150 (2%)
Frame = +1
Query: 217 LYIDGQYVDSRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT---WSKSTV 387
L+I+ Q+VDS +++ +P+T ++I V +A ++++ A+ A+ A++T W K +
Sbjct: 25 LFINNQHVDSVHGGRVKVYSPSTEKLICEVADADEEDVDIAVKVARAAFQTDAPWRKFSS 84
Query: 388 LTRQQLMFKFARLLRENQSKLAAKITEEQGKTIADAEGDVLRGIQSVEHCCSITSLQLGD 567
R + + + A + +N LA+ T + GK+I A GDV + G
Sbjct: 85 AQRGRCLSRLADCIEQNLEYLASIETLDNGKSITLARGDVQAAADCFRYYGGWADKDYGQ 144
Query: 568 SIQNIAKDMDTHSYKVPLGSCWRCCXLQFP 657
+I+ K ++ P+G C + FP
Sbjct: 145 TIETDIKRF-AYTRHEPIGVCGQIIPWNFP 173
Score = 52.4 bits (120), Expect = 8e-08
Identities = 22/69 (31%), Positives = 36/69 (52%)
Frame = +2
Query: 620 LGVVGGVAXFNFPVMIPLWMFPPALVTGNXCIIXPSXQDPXXTLMMMELLXEXXAPPGVV 799
+GV G + +NFP ++ W PA+ GN I+ + P L + + + E PPGV+
Sbjct: 161 IGVCGQIIPWNFPFLMCAWKIAPAVACGNTIILKTAELTPLSALCLTKFVPECGFPPGVI 220
Query: 800 NIIPGTTXR 826
N++ G R
Sbjct: 221 NVLSGDGRR 229
>SPCC550.10 |meu8||betaine aldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 3|||Manual
Length = 500
Score = 53.2 bits (122), Expect = 5e-08
Identities = 23/65 (35%), Positives = 36/65 (55%)
Frame = +2
Query: 623 GVVGGVAXFNFPVMIPLWMFPPALVTGNXCIIXPSXQDPXXTLMMMELLXEXXAPPGVVN 802
GV+G + +NFP+ + LW PA+ +GN ++ PS P L + E P GV+N
Sbjct: 164 GVIGVITPWNFPLKMALWKLVPAIASGNCVVLKPSELAPWSCLEFALICKEAGLPDGVLN 223
Query: 803 IIPGT 817
+I G+
Sbjct: 224 VIIGS 228
Score = 52.0 bits (119), Expect = 1e-07
Identities = 34/106 (32%), Positives = 54/106 (50%), Gaps = 4/106 (3%)
Frame = +1
Query: 202 APTTKLYIDGQYVD--SRTTNWIELTNPATNEVIGRVPEATQDELTSALDAAKRAYKT-- 369
AP L+IDG++V I L NPAT E+IG A+ ++ SA++ A +++
Sbjct: 18 APENSLFIDGKFVSPIEPAAKPIPLINPATEEIIGTCANASAKDVDSAVENAYNTFRSGI 77
Query: 370 WSKSTVLTRQQLMFKFARLLRENQSKLAAKITEEQGKTIADAEGDV 507
W+K R ++ K A+++RE + LA T GK A D+
Sbjct: 78 WAKWPGKQRGLVLRKIAKMMREKRELLAGIDTINCGKPTPYALFDI 123
>SPAC1002.12c |||succinate-semialdehyde dehydrogenase
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 498
Score = 39.1 bits (87), Expect = 8e-04
Identities = 19/63 (30%), Positives = 31/63 (49%)
Frame = +2
Query: 620 LGVVGGVAXFNFPVMIPLWMFPPALVTGNXCIIXPSXQDPXXTLMMMELLXEXXAPPGVV 799
+GVVG + +NFP + AL G +I P+ + P L + +L P GV+
Sbjct: 160 VGVVGIITPWNFPAAMITRKVGAALAAGCTVVIRPAAETPFTALALAKLAERAGVPAGVL 219
Query: 800 NII 808
N++
Sbjct: 220 NMV 222
>SPBC21C3.15c |||aldehyde dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 522
Score = 38.3 bits (85), Expect = 0.001
Identities = 19/85 (22%), Positives = 40/85 (47%), Gaps = 1/85 (1%)
Frame = +1
Query: 277 PATNEVIGRVPEATQDELTSALDAAKRAYKTWSKSTVLTRQQLMFKFARLLRENQSKLAA 456
P ++G V + ++ ++ A+ A K W ++ R+ + + NQ K A
Sbjct: 10 PGDGSLLGEVKLFNKSDIDQSIILAEEAQKEWKSTSFAERRNFLKALKENIIRNQDKYAE 69
Query: 457 KITEEQGKTIADAE-GDVLRGIQSV 528
++ GKT+ DA G++L ++ +
Sbjct: 70 IACKDTGKTLVDAAFGEILVTLEKI 94
>SPCC1753.05 |rsm1||RNA export factor Rsm1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 296
Score = 31.9 bits (69), Expect = 0.13
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 3/45 (6%)
Frame = +1
Query: 271 TNPATNEVIG---RVPEATQDELTSALDAAKRAYKTWSKSTVLTR 396
T+ TNE++ ++ E +D L +L A+K YK WS+ L R
Sbjct: 5 TDMETNEILDQLDKIDERNEDILLKSLKASKCTYKPWSREEFLRR 49
>SPAC13G7.01c |erg7|SPAC4G9.21c|lanosterol synthase Erg7
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 721
Score = 30.7 bits (66), Expect = 0.29
Identities = 18/44 (40%), Positives = 26/44 (59%), Gaps = 1/44 (2%)
Frame = +1
Query: 235 YVDSRTTNWIELTNPATNEVIGRV-PEATQDELTSALDAAKRAY 363
Y +RT W+EL NPA EV G + E + E T+++ A RA+
Sbjct: 498 YEPARTGEWMELLNPA--EVFGNIMVEYSYPECTTSVILALRAF 539
>SPBC24C6.04 |||delta-1-pyrroline-5-carboxylate
dehydrogenase|Schizosaccharomyces pombe|chr 2|||Manual
Length = 548
Score = 30.7 bits (66), Expect = 0.29
Identities = 17/64 (26%), Positives = 28/64 (43%)
Frame = +2
Query: 623 GVVGGVAXFNFPVMIPLWMFPPALVTGNXCIIXPSXQDPXXTLMMMELLXEXXAPPGVVN 802
G V + FNF + P L+ GN ++ PS + ++ ++ E P G +
Sbjct: 184 GFVYAITPFNFTAIAGNLAAAPLLM-GNVVLMKPSDHAVLSSYIVYQIFREAGLPAGALQ 242
Query: 803 IIPG 814
IPG
Sbjct: 243 FIPG 246
>SPAC2F7.11 |nrd1|msa2|RNA-binding protein Nrd1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 529
Score = 27.1 bits (57), Expect = 3.6
Identities = 11/32 (34%), Positives = 17/32 (53%)
Frame = +2
Query: 620 LGVVGGVAXFNFPVMIPLWMFPPALVTGNXCI 715
+GVVGG +P P+ P ++TGN +
Sbjct: 294 VGVVGGYPVMGYPPPSPVLQKPDLIMTGNRTV 325
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,132,233
Number of Sequences: 5004
Number of extensions: 59825
Number of successful extensions: 137
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 124
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 135
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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