BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_C20
(928 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 32 0.100
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 30 0.40
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 30 0.53
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 29 0.70
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 29 0.93
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 29 0.93
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.1
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 27 2.8
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.3 bits (70), Expect = 0.100
Identities = 32/124 (25%), Positives = 34/124 (27%), Gaps = 10/124 (8%)
Frame = +2
Query: 572 LXPXXXXXPPPXPTXXXPXVSPPXXPPPCAX-------PAXXPXXXXRXT--CPXXXXFX 724
L P PPP P P S PPP + PA P R P
Sbjct: 353 LPPQGRSAPPPPPPRSAP--STGRQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNAS 410
Query: 725 XXPGPPXPXPPXXXXSSXXXXXXPXPPXPXXXPXXXPPXXXXXXXXPPXXXXXXXXXP-P 901
PP P PP S+ PP PP PP P P
Sbjct: 411 RTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLP 470
Query: 902 PXXP 913
P P
Sbjct: 471 PAAP 474
Score = 28.3 bits (60), Expect = 1.6
Identities = 26/120 (21%), Positives = 32/120 (26%), Gaps = 3/120 (2%)
Frame = +2
Query: 563 SPXLXPXXXXXPPPXPTXXXPXVSPPXXPPPC-AXPAXXPXXXXRXTCP--XXXXFXXXP 733
+P + P PP +PP PPP + P R P
Sbjct: 229 APPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTS 288
Query: 734 GPPXPXPPXXXXSSXXXXXXPXPPXPXXXPXXXPPXXXXXXXXPPXXXXXXXXXPPPXXP 913
PP P P ++ PP P PP P PPP P
Sbjct: 289 KPPLPPPSSRVSAAALAANKKRPPPP------PPPSRRNRGKPPIGNGSSNSSLPPPPPP 342
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/70 (27%), Positives = 19/70 (27%)
Frame = +3
Query: 699 PVXPXPPSXXXLXPPXPXPPPXXXXPXXXXXXXPPXXXPXXPXXXPPPXXXXXXXPPXXX 878
P P PPS PP P P P P P P P P
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPP--LPPSAPIAPPLPAGMPAAPPLPPA 472
Query: 879 XPXPXPXXXP 908
P P P P
Sbjct: 473 APAPPPAPAP 482
Score = 26.2 bits (55), Expect = 6.6
Identities = 26/106 (24%), Positives = 28/106 (26%), Gaps = 2/106 (1%)
Frame = +2
Query: 557 RXSPXLXPXXXXXPPPXPTXXXPXVSPPXXPP-PCAXPAXXPXXXXRXTCPXXXXFXXXP 733
R P L PP P P S P PP A P + P P
Sbjct: 374 RQPPPLSSSRAVSNPPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPP 433
Query: 734 GPPXPXPPXXXXSSXXXXXXP-XPPXPXXXPXXXPPXXXXXXXXPP 868
P P + P PP P P PP PP
Sbjct: 434 SAPPSLPMGAPAAPPLPPSAPIAPPLPAGMP-AAPPLPPAAPAPPP 478
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 30.3 bits (65), Expect = 0.40
Identities = 18/81 (22%), Positives = 21/81 (25%)
Frame = +2
Query: 596 PPPXPTXXXPXVSPPXXPPPCAXPAXXPXXXXRXTCPXXXXFXXXPGPPXPXPPXXXXSS 775
P P P+ P + P PP P+ P PP P P
Sbjct: 1065 PVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVP 1124
Query: 776 XXXXXXPXPPXPXXXPXXXPP 838
P P P P P
Sbjct: 1125 KPSVAAPPVPVPSGAPPVPKP 1145
Score = 30.3 bits (65), Expect = 0.40
Identities = 25/105 (23%), Positives = 28/105 (26%)
Frame = +1
Query: 598 PPXPXXXXTXRFPPXXXPSLRXPCXXPXPXXPXYLSXLXXLPXTPWXPXXLPPXRXXXXX 777
PP P PP PS P P P + +P P +PP
Sbjct: 1140 PPVPKPSVAA--PPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPV-----P 1192
Query: 778 XPXXXPXPPXXXXXPPXXPPXXXXXXXXXPXXXXXPXPXPXXXXP 912
P P P P PP P P P P P
Sbjct: 1193 PPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAGLPPVPVPTAKAP 1237
Score = 29.9 bits (64), Expect = 0.53
Identities = 26/105 (24%), Positives = 26/105 (24%)
Frame = +1
Query: 598 PPXPXXXXTXRFPPXXXPSLRXPCXXPXPXXPXYLSXLXXLPXTPWXPXXLPPXRXXXXX 777
PP P PP PS P P P S P P P P
Sbjct: 1022 PPVPLPSADA--PPIPVPSTAPPVPIPTSTPPVPKSS-SGAPSAP-PPVPAPSSEIPSIP 1077
Query: 778 XPXXXPXPPXXXXXPPXXPPXXXXXXXXXPXXXXXPXPXPXXXXP 912
P P P PP P P P P P P
Sbjct: 1078 APSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPP 1122
Score = 29.5 bits (63), Expect = 0.70
Identities = 24/103 (23%), Positives = 26/103 (25%)
Frame = +2
Query: 596 PPPXPTXXXPXVSPPXXPPPCAXPAXXPXXXXRXTCPXXXXFXXXPGPPXPXPPXXXXSS 775
P P P+ P + P PP P P P P P P P
Sbjct: 1023 PVPLPSADAPPIPVPSTAPPVPIPTSTP--------PVPKSSSGAPSAPPPVPAPSSEIP 1074
Query: 776 XXXXXXPXPPXPXXXPXXXPPXXXXXXXXPPXXXXXXXXXPPP 904
PP P P PP PP P P
Sbjct: 1075 SIPAPSGAPPVP--APSGIPPVPKPSVAAPPVPKPSVAVPPVP 1115
Score = 29.5 bits (63), Expect = 0.70
Identities = 12/35 (34%), Positives = 15/35 (42%)
Frame = +2
Query: 566 PXLXPXXXXXPPPXPTXXXPXVSPPXXPPPCAXPA 670
P + P P P P+ P V PP PP P+
Sbjct: 1189 PPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPS 1223
Score = 27.9 bits (59), Expect = 2.1
Identities = 19/64 (29%), Positives = 20/64 (31%), Gaps = 1/64 (1%)
Frame = +2
Query: 566 PXLXPXXXXXPPPXPTXXXPXVSPPXXPPPCAXPA-XXPXXXXRXTCPXXXXFXXXPGPP 742
P P P P P P V PP PP P+ P T P PP
Sbjct: 1170 PVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPT-PSAGLPP 1228
Query: 743 XPXP 754
P P
Sbjct: 1229 VPVP 1232
Score = 27.5 bits (58), Expect = 2.8
Identities = 26/132 (19%), Positives = 33/132 (25%)
Frame = +1
Query: 517 PRCXRFSXXSPPLTXLPXTXXSXXPVXPPXPXXXXTXRFPPXXXPSLRXPCXXPXPXXPX 696
P+ + +PP P + P P PP PS+ P P P
Sbjct: 1053 PKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAP---PVPKPSV 1109
Query: 697 YLSXLXXLPXTPWXPXXLPPXRXXXXXXPXXXPXPPXXXXXPPXXPPXXXXXXXXXPXXX 876
+ + P P P P P P P P P
Sbjct: 1110 AVPPVPAPSGAP--PVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVA 1167
Query: 877 XXPXPXPXXXXP 912
P P P P
Sbjct: 1168 APPVPAPSSGIP 1179
Score = 27.5 bits (58), Expect = 2.8
Identities = 21/101 (20%), Positives = 24/101 (23%)
Frame = +2
Query: 566 PXLXPXXXXXPPPXPTXXXPXVSPPXXPPPCAXPAXXPXXXXRXTCPXXXXFXXXPGPPX 745
P P P P P+ P V P PP P+ + PP
Sbjct: 1093 PVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPV 1152
Query: 746 PXPPXXXXSSXXXXXXPXPPXPXXXPXXXPPXXXXXXXXPP 868
P P P P P P PP
Sbjct: 1153 PAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193
Score = 26.2 bits (55), Expect = 6.6
Identities = 22/110 (20%), Positives = 26/110 (23%), Gaps = 1/110 (0%)
Frame = +2
Query: 578 PXXXXXPPPXPTXXXPXVSPPXXPPPCAXPAXXPXXXXRXTCPXXXXFXXXPGPPXPXPP 757
P PP + +PP P P + P P P P PP
Sbjct: 1044 PIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPP 1103
Query: 758 XXXXS-SXXXXXXPXPPXPXXXPXXXPPXXXXXXXXPPXXXXXXXXXPPP 904
S + P P P P PP P P
Sbjct: 1104 VPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVP 1153
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.9 bits (64), Expect = 0.53
Identities = 11/23 (47%), Positives = 11/23 (47%)
Frame = +3
Query: 693 GXPVXPXPPSXXXLXPPXPXPPP 761
G P P PP PP P PPP
Sbjct: 760 GPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/55 (32%), Positives = 19/55 (34%), Gaps = 1/55 (1%)
Frame = +2
Query: 596 PPPXPTXXXPXVSPPXXP-PPCAXPAXXPXXXXRXTCPXXXXFXXXPGPPXPXPP 757
PPP P P +P P PP A P P P PP P PP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPP----PPPGVAGAGPPPPPPPPP 783
Score = 27.9 bits (59), Expect = 2.1
Identities = 13/45 (28%), Positives = 14/45 (31%)
Frame = +3
Query: 708 PXPPSXXXLXPPXPXPPPXXXXPXXXXXXXPPXXXPXXPXXXPPP 842
P PP + P P P P PP P PPP
Sbjct: 733 PPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPP 777
Score = 27.9 bits (59), Expect = 2.1
Identities = 18/70 (25%), Positives = 19/70 (27%), Gaps = 3/70 (4%)
Frame = +3
Query: 708 PXPPSXXXLXP---PXPXPPPXXXXPXXXXXXXPPXXXPXXPXXXPPPXXXXXXXPPXXX 878
P PP+ P P P PPP PP P PPP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYY 793
Query: 879 XPXPXPXXXP 908
P P P
Sbjct: 794 APAPQAEPEP 803
Score = 27.5 bits (58), Expect = 2.8
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +2
Query: 566 PXLXPXXXXXPPPXPTXXXPXVSPPXXPPP 655
P P PPP P PP PPP
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.8
Identities = 15/53 (28%), Positives = 19/53 (35%)
Frame = +1
Query: 547 PPLTXLPXTXXSXXPVXPPXPXXXXTXRFPPXXXPSLRXPCXXPXPXXPXYLS 705
PP +P + PV PP P PP P + P P P +S
Sbjct: 736 PPAVIVPTPAPAPIPVPPPAPIMGGPP--PPPPPPGVAGAGPPPPPPPPPAVS 786
Score = 26.6 bits (56), Expect = 5.0
Identities = 15/52 (28%), Positives = 15/52 (28%), Gaps = 1/52 (1%)
Frame = +3
Query: 738 PPXPXPPPXXXXP-XXXXXXXPPXXXPXXPXXXPPPXXXXXXXPPXXXXPXP 890
PP P P P PP P PPP PP P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/35 (31%), Positives = 13/35 (37%)
Frame = +3
Query: 699 PVXPXPPSXXXLXPPXPXPPPXXXXPXXXXXXXPP 803
P+ PP+ PP P PPP PP
Sbjct: 748 PIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.5 bits (63), Expect = 0.70
Identities = 17/50 (34%), Positives = 18/50 (36%), Gaps = 2/50 (4%)
Frame = +3
Query: 699 PVXPXPPSXXX-LXPPXPXPPPXXXXPXXXXXXXP-PXXXPXXPXXXPPP 842
P P PP+ L PP PP P P P P P PPP
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPP 174
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 29.1 bits (62), Expect = 0.93
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +2
Query: 578 PXXXXXPPPXPTXXXPXVSPPXXPPP 655
P PPP P P PP PPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 26.6 bits (56), Expect = 5.0
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +2
Query: 572 LXPXXXXXPPPXPTXXXPXVSPPXXPP 652
L P PPP P P PP PP
Sbjct: 4 LPPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 29.1 bits (62), Expect = 0.93
Identities = 20/81 (24%), Positives = 22/81 (27%)
Frame = +2
Query: 596 PPPXPTXXXPXVSPPXXPPPCAXPAXXPXXXXRXTCPXXXXFXXXPGPPXPXPPXXXXSS 775
PPP P P PP P A P P PG P P P ++
Sbjct: 1882 PPPPPPMALPKAGPPSAAPTSALPPAGPP-------AGATAISGNPGMPAPVPLTGKETA 1934
Query: 776 XXXXXXPXPPXPXXXPXXXPP 838
P P PP
Sbjct: 1935 VPLSSMPNAPPSVASNAKLPP 1955
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.1
Identities = 16/48 (33%), Positives = 18/48 (37%)
Frame = +3
Query: 699 PVXPXPPSXXXLXPPXPXPPPXXXXPXXXXXXXPPXXXPXXPXXXPPP 842
PV P + + P P PPP P PP P P PPP
Sbjct: 1691 PVRPQSAAPPQMSAPTPPPPPMSVPP---PPSAPP--MPAGPPSAPPP 1733
Score = 26.6 bits (56), Expect = 5.0
Identities = 13/30 (43%), Positives = 13/30 (43%), Gaps = 2/30 (6%)
Frame = +2
Query: 596 PPPXPTXXXPXVSPPXXP--PPCAXPAXXP 679
PPP P P S P P PP A P P
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 27.5 bits (58), Expect = 2.8
Identities = 20/67 (29%), Positives = 20/67 (29%)
Frame = -1
Query: 907 GXXXGXGXGXXXXGGXXXXXXXGGGXXXGXXGXXXGGXXXXXXXGXXXXGGGXGXGGXRX 728
G G G GG GG G G GG G GGG G G
Sbjct: 208 GGFGGFGGEGHHHGGHGGFGGGPGGFEGGPGGF--GGGPGGFGGGLGGFGGGPGGFGGGP 265
Query: 727 XXEGGXG 707
GG G
Sbjct: 266 GGHGGPG 272
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,619,391
Number of Sequences: 5004
Number of extensions: 20525
Number of successful extensions: 168
Number of sequences better than 10.0: 8
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 115
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 469338710
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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