Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_C18
(900 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 243 2e-65
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 116 6e-27
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 81 2e-16
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 77 3e-15
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 46 7e-06
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 43 7e-05
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 39 8e-04
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 39 0.001
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 29 0.68
SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase Y|Schizosacch... 26 6.3
SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1 |... 26 8.4
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 243 bits (595), Expect = 2e-65
Identities = 127/210 (60%), Positives = 138/210 (65%)
Frame = +1
Query: 247 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHP 426
+MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP
Sbjct: 43 IMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHP 102
Query: 427 VLLTEAPLNPKANRGEDDPDHV*DLQLXXXXXXXXXXXXXXXXXXXXXXXXXXXEMVSPT 606
LLTEAPLNPK+NR + + V+ T
Sbjct: 103 CLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHT 162
Query: 607 PYPSTKVTLCPTPSSXLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEXLCYVAL 786
P + P LDLAGRDLTDYLMKIL ERGY+F+TTA REIVRDIKE LCYVAL
Sbjct: 163 -VPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEKLCYVAL 221
Query: 787 DFXQXMATXXXSTXLXXSXXLPDGQVXPIG 876
DF Q + T S+ L S LPDGQV IG
Sbjct: 222 DFEQELQTAAQSSSLEKSYELPDGQVITIG 251
Score = 96.3 bits (229), Expect = 5e-21
Identities = 45/51 (88%), Positives = 47/51 (92%)
Frame = +3
Query: 510 PXMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRSGL 662
P YVAIQAVLSLYASGRTTGIVLDSGDGV+HTVPIYEGYALPHAI+R L
Sbjct: 130 PAFYVAIQAVLSLYASGRTTGIVLDSGDGVTHTVPIYEGYALPHAIMRLDL 180
Score = 84.2 bits (199), Expect = 2e-17
Identities = 35/41 (85%), Positives = 40/41 (97%)
Frame = +3
Query: 123 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG 245
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHG 42
Score = 30.7 bits (66), Expect = 0.29
Identities = 12/14 (85%), Positives = 14/14 (100%)
Frame = +2
Query: 470 EKMTQIMFETFNSP 511
EKMTQI+FETFN+P
Sbjct: 117 EKMTQIIFETFNAP 130
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 116 bits (278), Expect = 6e-27
Identities = 70/211 (33%), Positives = 102/211 (48%), Gaps = 1/211 (0%)
Frame = +1
Query: 247 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEH 423
VM QKD +VG EAQ+ RG+L ++ PIE GII NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 424 PVLLTEAPLNPKANRGEDDPDHV*DLQLXXXXXXXXXXXXXXXXXXXXXXXXXXXEMVSP 603
P+LLTE PL N+ + L + + ++
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECGDGLTH 166
Query: 604 TPYPSTKVTLCPTPSSXLDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEXLCYVA 783
+ P P+ ++ GRD+TDYL L + G+ ++A +EIVR+IKE CYVA
Sbjct: 167 S-VPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEKCCYVA 225
Query: 784 LDFXQXMATXXXSTXLXXSXXLPDGQVXPIG 876
DF + + + LPD Q +G
Sbjct: 226 SDFRSEIESWTEHKPQIHTYQLPDNQTITLG 256
Score = 67.7 bits (158), Expect = 2e-12
Identities = 28/52 (53%), Positives = 42/52 (80%)
Frame = +3
Query: 510 PXMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRSGLG 665
P + ++Q VL+LYAS RTTGIVL+ GDG++H+VPIY+G+++P AI + +G
Sbjct: 135 PALSFSLQPVLALYASARTTGIVLECGDGLTHSVPIYDGFSIPSAIQQEEIG 186
Score = 52.8 bits (121), Expect = 6e-08
Identities = 21/38 (55%), Positives = 28/38 (73%)
Frame = +3
Query: 129 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQ 242
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHE 45
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 81.0 bits (191), Expect = 2e-16
Identities = 32/67 (47%), Positives = 51/67 (76%)
Frame = +1
Query: 268 KDSYVGDEAQSKRGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 447
KD VGDEA++ R +L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 448 LNPKANR 468
+NP ANR
Sbjct: 110 MNPVANR 116
Score = 69.3 bits (162), Expect = 7e-13
Identities = 33/74 (44%), Positives = 45/74 (60%)
Frame = +1
Query: 655 LDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEXLCYVALDFXQXMATXXXSTXLX 834
LD+AGRD T YL+ +L +GY+F TA E VR++KE LCYV+ D +T L
Sbjct: 178 LDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEKLCYVSYDLELDHKLSEETTVLM 237
Query: 835 XSXXLPDGQVXPIG 876
+ LPDG+V +G
Sbjct: 238 RNYTLPDGRVIKVG 251
Score = 66.1 bits (154), Expect = 6e-12
Identities = 29/46 (63%), Positives = 37/46 (80%)
Frame = +3
Query: 516 MYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILR 653
+YVAIQAVLSLYA G ++G+V+DSGDGV+H VP+YE L H + R
Sbjct: 132 VYVAIQAVLSLYAQGLSSGVVVDSGDGVTHIVPVYESVVLNHLVGR 177
Score = 46.8 bits (106), Expect = 4e-06
Identities = 20/33 (60%), Positives = 25/33 (75%)
Frame = +3
Query: 135 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP 233
A +V+DNG+G K G+A D+ PR FPSIVGRP
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRP 36
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 77.0 bits (181), Expect = 3e-15
Identities = 40/98 (40%), Positives = 55/98 (56%), Gaps = 4/98 (4%)
Frame = +1
Query: 187 ATTRPAPSSRPSWVALATRXVMVGMGQK---DSYVGDEAQSKRGI-LTLKYPIEHGIITN 354
A++ PA SS+PS++A + D ++G++A K +L YPI HG I N
Sbjct: 42 ASSGPAVSSKPSYMASKGSGHLSSKRATEDLDFFIGNDALKKASAGYSLDYPIRHGQIEN 101
Query: 355 WDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 468
WD ME+ W + + LR PE+H LLTE PLNP NR
Sbjct: 102 WDHMERFWQQSLFKYLRCEPEDHYFLLTEPPLNPPENR 139
Score = 47.6 bits (108), Expect = 2e-06
Identities = 24/52 (46%), Positives = 35/52 (67%), Gaps = 8/52 (15%)
Frame = +3
Query: 516 MYVAIQAVLSLYASGRT--------TGIVLDSGDGVSHTVPIYEGYALPHAI 647
+Y+A+QAVL+L AS + TG V+DSGDGV+H +P+ EGY + +I
Sbjct: 155 LYIAVQAVLALAASWTSSKVTDRSLTGTVVDSGDGVTHIIPVAEGYVIGSSI 206
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +3
Query: 141 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 224
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
Score = 28.3 bits (60), Expect = 1.6
Identities = 16/45 (35%), Positives = 24/45 (53%)
Frame = +1
Query: 655 LDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEXLCYVALD 789
+ LAGRD+T ++ +L +R ++ E IKE CYV D
Sbjct: 209 MPLAGRDVTYFVQSLLRDRNEPDSSLKTAE---RIKEECCYVCPD 250
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 46.0 bits (104), Expect = 7e-06
Identities = 19/52 (36%), Positives = 29/52 (55%)
Frame = +1
Query: 313 LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 468
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENR 114
Score = 41.5 bits (93), Expect = 2e-04
Identities = 23/60 (38%), Positives = 35/60 (58%), Gaps = 2/60 (3%)
Frame = +3
Query: 126 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGPERLLRG*RGPE 299
D+V+A+V+D GS + GF+G+D P+ V PS G + DGR +G E + + G E
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYIYKSNPGME 64
Score = 31.9 bits (69), Expect = 0.13
Identities = 15/42 (35%), Positives = 23/42 (54%)
Frame = +3
Query: 510 PXMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYAL 635
P Y+A Q + +ASG+ T ++D G S IY+G+ L
Sbjct: 128 PATYLAKQETCAAFASGKGTACLVDIGAERSSVSAIYDGFVL 169
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 42.7 bits (96), Expect = 7e-05
Identities = 21/67 (31%), Positives = 39/67 (58%), Gaps = 1/67 (1%)
Frame = +1
Query: 259 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLL 435
+ + + VG++ + G ++ + P E +I+NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 436 TEAPLNP 456
TE NP
Sbjct: 121 TEPLANP 127
Score = 37.5 bits (83), Expect = 0.003
Identities = 21/58 (36%), Positives = 34/58 (58%)
Frame = +3
Query: 63 AAVESIPASPTQTHTKFKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 236
A E + + PT + F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 5 AVREPVFSGPTPS---FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 29.5 bits (63), Expect = 0.68
Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 2/57 (3%)
Frame = +3
Query: 510 PXMYVAIQAVLSLYASGR--TTGIVLDSGDGVSHTVPIYEGYALPHAILRSGLGWSR 674
P + I + S Y + + ++GIVL+ G+ SH +P+ G + R G S+
Sbjct: 145 PSVAYGIDGLFSFYHNTKPSSSGIVLNLGNAASHVIPVLNGERILSEAKRISWGGSQ 201
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 39.1 bits (87), Expect = 8e-04
Identities = 21/69 (30%), Positives = 36/69 (52%), Gaps = 2/69 (2%)
Frame = +1
Query: 268 KDSYVGDEAQSKRGI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTE 441
++ YV DE Q I + +K +GII +W+ W +L+V P E+ +++TE
Sbjct: 49 RNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITE 108
Query: 442 APLNPKANR 468
NP++ R
Sbjct: 109 PSWNPQSVR 117
Score = 34.3 bits (75), Expect = 0.024
Identities = 12/34 (35%), Positives = 23/34 (67%)
Frame = +3
Query: 126 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG 227
+++ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 38.7 bits (86), Expect = 0.001
Identities = 18/50 (36%), Positives = 32/50 (64%)
Frame = +1
Query: 655 LDLAGRDLTDYLMKILTERGYSFTTTAXREIVRDIKEXLCYVALDFXQXM 804
+D+ G+ LT+YL ++++ R Y+ IV +IKE +CYV+ +F + M
Sbjct: 186 VDVGGKLLTNYLKEVISYRKYNMMEETY--IVNEIKESVCYVSQNFKEDM 233
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 29.5 bits (63), Expect = 0.68
Identities = 16/52 (30%), Positives = 26/52 (50%)
Frame = +3
Query: 510 PXMYVAIQAVLSLYASGRTTGIVLDSGDGVSHTVPIYEGYALPHAILRSGLG 665
P +A + ++ LYA G G+V+D G + PI +G + A + LG
Sbjct: 176 PGFTIAYEPLMGLYAIGILHGLVIDIGYEKTDITPILDGQIIFTATQQLPLG 227
>SPAC19G12.10c |cpy1|pcy1|vacuolar carboxypeptidase
Y|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1002
Score = 26.2 bits (55), Expect = 6.3
Identities = 15/38 (39%), Positives = 17/38 (44%)
Frame = -1
Query: 351 GDDTVLDGVLQSEDTSLALGLVTHVGVFLAHTDHHXPG 238
GDD+VLD V +D L L AH D H G
Sbjct: 676 GDDSVLDTVTAGKDVYAFLNLFFAKFPQYAHLDFHIAG 713
>SPBC23E6.08 |sat1||Golgi membrane exchange factor subunit Sat1
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 550
Score = 25.8 bits (54), Expect = 8.4
Identities = 11/31 (35%), Positives = 16/31 (51%)
Frame = -3
Query: 583 SSTIPVVRPEAYSESTAWMATYMXGGVEGLK 491
+ TI V P + WM M GG+EG++
Sbjct: 518 AETIQCVLPIRIEKRVVWMEDGMEGGMEGMQ 548
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,159,995
Number of Sequences: 5004
Number of extensions: 60810
Number of successful extensions: 208
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 173
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 205
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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