Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_A24
(963 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces po... 137 2e-33
SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces po... 133 3e-32
SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFII... 30 0.42
SPBC21.01 |mis17|SPBC776.19|kinetochore protein Mis17|Schizosacc... 29 0.98
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 1.3
SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr... 26 6.9
SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|... 26 9.1
>SPAC8E11.02c |rad24||14-3-3 protein Rad24|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 137 bits (331), Expect = 2e-33
Identities = 71/108 (65%), Positives = 87/108 (80%)
Frame = +2
Query: 113 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 292
+ RED VY AKLAEQAERY+ MVE MK+VAS +D ELTVEERNLLSVAYKNVIGARRA
Sbjct: 4 TSREDAVYLAKLAEQAERYEGMVENMKSVAS---TDQELTVEERNLLSVAYKNVIGARRA 60
Query: 293 SFXIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDXLGVLDQ 436
S+ I+SSIEQKEE+KG ++ +I+ YR ++E+EL IC D L VL++
Sbjct: 61 SWRIVSSIEQKEESKGNTAQVELIKEYRQKIEQELDTICQDILTVLEK 108
Score = 61.7 bits (143), Expect = 1e-10
Identities = 26/41 (63%), Positives = 33/41 (80%)
Frame = +3
Query: 435 KYLIPSSQTGESXVFYYXMKGDYHRYLAEFATGHDRQEAAE 557
K+LIP++ + ES VFYY MKGDY+RYLAEFA G RQ +A+
Sbjct: 108 KHLIPNAASAESKVFYYKMKGDYYRYLAEFAVGEKRQHSAD 148
>SPAC17A2.13c |rad25||14-3-3 protein Rad25|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 270
Score = 133 bits (322), Expect = 3e-32
Identities = 70/115 (60%), Positives = 88/115 (76%)
Frame = +2
Query: 113 SEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRA 292
+ RE++VY AKLAEQAERY+EMVE MK VA N ++L+VEERNLLSVAYKN+IGARRA
Sbjct: 3 NSRENSVYLAKLAEQAERYEEMVENMKKVACSN---DKLSVEERNLLSVAYKNIIGARRA 59
Query: 293 SFXIISSIEQKEETKGAEDKLNMIRAYRSQVEKELRDICSDXLGVLDQVPHPQLT 457
S+ IISSIEQKEE++G + +I+ YR ++E EL DIC D L VL++ P T
Sbjct: 60 SWRIISSIEQKEESRGNTRQAALIKEYRKKIEDELSDICHDVLSVLEKHLIPAAT 114
Score = 62.1 bits (144), Expect = 1e-10
Identities = 27/42 (64%), Positives = 34/42 (80%)
Frame = +3
Query: 435 KYLIPSSQTGESXVFYYXMKGDYHRYLAEFATGHDRQEAAEN 560
K+LIP++ TGES VFYY MKGDY+RYLAEF G +EAA++
Sbjct: 107 KHLIPAATTGESKVFYYKMKGDYYRYLAEFTVGEVCKEAADS 148
>SPAC2G11.14 |taf111|taf1, taf1, taf130|transcription factor TFIID
complex subunit Taf111|Schizosaccharomyces pombe|chr
1|||Manual
Length = 979
Score = 30.3 bits (65), Expect = 0.42
Identities = 21/85 (24%), Positives = 38/85 (44%)
Frame = +2
Query: 128 NVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASFXII 307
N + +A+Q Y+E + + N R +S N NL +A K I + +
Sbjct: 779 NAHAYNVAKQQRAYEEEINRIWNAQKRGLSIN-------NLEELAKKYGINSIHDDYVES 831
Query: 308 SSIEQKEETKGAEDKLNMIRAYRSQ 382
+ +EET ++ L ++R YR +
Sbjct: 832 NEETTREETPSSDKVLRIVRLYRDK 856
>SPBC21.01 |mis17|SPBC776.19|kinetochore protein
Mis17|Schizosaccharomyces pombe|chr 2|||Manual
Length = 441
Score = 29.1 bits (62), Expect = 0.98
Identities = 23/100 (23%), Positives = 46/100 (46%)
Frame = +2
Query: 101 SSTMSEREDNVYKAKLAEQAERYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIG 280
SST S N ++ Q+E Y ++ + + + ++SD E +E R V +N +
Sbjct: 45 SSTRSSPASNQQNLEIVSQSEAYHKISDLDDSTSIFSISDQENILERRISEEVVRENSV- 103
Query: 281 ARRASFXIISSIEQKEETKGAEDKLNMIRAYRSQVEKELR 400
+S +SS ET+ + + L + S +K+++
Sbjct: 104 ---SSIENVSSSAVARETQSSANILVKDNHFFSHKQKKIQ 140
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.7 bits (61), Expect = 1.3
Identities = 11/21 (52%), Positives = 11/21 (52%)
Frame = +1
Query: 892 PAXPPTPXPXXXPPPXTAPXP 954
PA PP P PPP AP P
Sbjct: 464 PAAPPLPPAAPAPPPAPAPAP 484
>SPAC1687.10 |mcp1||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 661
Score = 26.2 bits (55), Expect = 6.9
Identities = 17/62 (27%), Positives = 30/62 (48%), Gaps = 4/62 (6%)
Frame = +2
Query: 131 VYKAKLAEQAE----RYDEMVEAMKNVASRNVSDNELTVEERNLLSVAYKNVIGARRASF 298
+YK KL E R DE+V MKN+ +N + +EL + ++N + + R +
Sbjct: 202 IYKEKLRFSLESRKSRVDEIVNEMKNMCGKNPT-SELPTNYSDKTLTLWENELSSMRKVY 260
Query: 299 XI 304
+
Sbjct: 261 FV 262
>SPCC830.03 |||AAA family ATPase Grc3 |Schizosaccharomyces pombe|chr
3|||Manual
Length = 736
Score = 25.8 bits (54), Expect = 9.1
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 2/43 (4%)
Frame = -2
Query: 230 SAHCRTRCVMLHSSSPPPFRHSAQPAQLTLLYT--HYLPFPTL 108
S+H + + H P F S QLTLL + YLP P +
Sbjct: 486 SSHREKKSIYQHKEYMPDFLSSRDEFQLTLLESTWQYLPDPNV 528
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,264,679
Number of Sequences: 5004
Number of extensions: 35257
Number of successful extensions: 152
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 123
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 150
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 493304942
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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