Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP12_F_A14
         (878 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   191   1e-49
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   185   6e-48
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...    98   2e-21
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    69   7e-13

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  191 bits (465), Expect = 1e-49
 Identities = 92/186 (49%), Positives = 113/186 (60%)
 Frame = +3

Query: 231 CLEXGIQPDGQMPTDXTXXXXXXSFNTFFSEXGAGXXVPRAVFVDLEPTVVDEVRTGTYX 410
           CLE GIQP+G M  +         F+TFFSE G G  VPR+++VDLEP V+D+VRTG Y 
Sbjct: 25  CLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYR 84

Query: 411 QLFXPEXLITGKEDAXNNYARGXYXIGKEIVXLVLDRIRXLADQCTGLQXXXXXXXXXXX 590
            LF PE LITGKEDA NNYARG Y +GKE+V  V D+IR +AD C+GLQ           
Sbjct: 85  DLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGG 144

Query: 591 XXXXXXXXXMXRLSVDYGXXSKLEFXIXPAPXVSTXVVXPYXSILTXHTXLXXXDCAFLV 770
                    + RL+++Y   SKL+F + PAP VST VV PY S+LT H  L   DC F+V
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHATLDLADCTFMV 204

Query: 771 XPXXLY 788
                Y
Sbjct: 205 DNESCY 210



 Score = 40.7 bits (91), Expect = 3e-04
 Identities = 16/24 (66%), Positives = 17/24 (70%)
 Frame = +2

Query: 158 MRXCXSVHVGXAGVQXGNACWXLY 229
           MR   S+HVG AG Q GNACW LY
Sbjct: 1   MREIISIHVGQAGTQIGNACWELY 24


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  185 bits (451), Expect = 6e-48
 Identities = 92/191 (48%), Positives = 115/191 (60%), Gaps = 5/191 (2%)
 Frame = +3

Query: 231 CLEXGIQPDGQMPTDXTXXXXXXS-----FNTFFSEXGAGXXVPRAVFVDLEPTVVDEVR 395
           CLE GI PDG  PT+ +      S     F TFFSE G G  VPR+++VDLEP V+D+VR
Sbjct: 25  CLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVR 83

Query: 396 TGTYXQLFXPEXLITGKEDAXNNYARGXYXIGKEIVXLVLDRIRXLADQCTGLQXXXXXX 575
           TG Y  LF PE ++TGKEDA NNYARG Y +GKE++  VL+RIR +AD C+GLQ      
Sbjct: 84  TGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143

Query: 576 XXXXXXXXXXXXXXMXRLSVDYGXXSKLEFXIXPAPXVSTXVVXPYXSILTXHTXLXXXD 755
                         + RL+++YG  S L+F + PAP VST VV PY S+LT H  L   D
Sbjct: 144 SFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHATLDNSD 203

Query: 756 CAFLVXPXXLY 788
           C F+V     Y
Sbjct: 204 CTFMVDNEACY 214



 Score = 42.7 bits (96), Expect = 7e-05
 Identities = 18/24 (75%), Positives = 18/24 (75%)
 Frame = +2

Query: 158 MRXCXSVHVGXAGVQXGNACWXLY 229
           MR   SVHVG AGVQ GNACW LY
Sbjct: 1   MREVISVHVGQAGVQIGNACWELY 24


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score = 97.9 bits (233), Expect = 2e-21
 Identities = 51/155 (32%), Positives = 74/155 (47%)
 Frame = +3

Query: 306 NTFFSEXGAGXXVPRAVFVDLEPTVVDEVRTGTYXQLFXPEXLITGKEDAXNNYARGXYX 485
           N +F+E   G  VPRAV VDLEP  +D V++G +  LF P+ +I G+  A N +A+G Y 
Sbjct: 48  NVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYT 107

Query: 486 IGKEIVXLVLDRIRXLADQCTGLQXXXXXXXXXXXXXXXXXXXXMXRLSVDYGXXSKLEF 665
            G E+   VLD +R  A+ C  LQ                    + ++  +Y       F
Sbjct: 108 EGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATF 167

Query: 666 XIXPAPXVSTXVVXPYXSILTXHTXLXXXDCAFLV 770
            + PAP  S  VV PY + L+ H  +   D  F +
Sbjct: 168 SVAPAPKSSDTVVEPYNATLSMHQLVENSDETFCI 202


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 69.3 bits (162), Expect = 7e-13
 Identities = 50/168 (29%), Positives = 74/168 (44%), Gaps = 3/168 (1%)
 Frame = +3

Query: 231 CLEXGIQPDGQMPTDXTXXXXXXSFNTFFSEXGAGXXVPRAVFVDLEPTVVDEVRTGTYX 410
           CLE GI PDG + +  T        + FF +      +PRA+ +DLEP VV+ + + TY 
Sbjct: 26  CLEHGIGPDGTLESFATEGVDRK--DVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83

Query: 411 QLFXPEXLITGKE--DAXNNYARGXYXIGKEIVXLVLDRIRXLADQCTGLQXXXXXXXXX 584
            L+ PE ++  K    A NN+A G Y   + I   ++D I   AD    L+         
Sbjct: 84  SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142

Query: 585 XXXXXXXXXXXMXRLSVDYGXXSKLEFXIXP-APXVSTXVVXPYXSIL 725
                      + RL+  Y       + + P +  VS  VV PY S+L
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVSDVVVQPYNSLL 190


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,979,088
Number of Sequences: 5004
Number of extensions: 25069
Number of successful extensions: 39
Number of sequences better than 10.0: 4
Number of HSP's better than 10.0 without gapping: 32
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 34
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 440481800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)