BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP12_F_A03
(934 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 34 0.025
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 30 0.41
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 1.2
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 1.6
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 3.5
SPAC23D3.10c |eng2||endo-1,3-beta-glucanase Eng2|Schizosaccharom... 26 6.6
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.6
SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 8.7
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 26 8.7
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 34.3 bits (75), Expect = 0.025
Identities = 30/139 (21%), Positives = 30/139 (21%), Gaps = 2/139 (1%)
Frame = +2
Query: 518 PPXXXGXXXPPXXKXPXXXXXXPPPPXXPXXXXXXXXXHXXXPP-PPXXXXPPXXPXXXX 694
PP PP K PPP P PP P PP
Sbjct: 1041 PPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVA 1100
Query: 695 XPP-PXXXXPXXXFPXPXXXXXXPXXXXXXXXXPXPXXXXPXXXXXXXXXXXPXPXPPXP 871
PP P P P P P P P P P P
Sbjct: 1101 APPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPP 1160
Query: 872 XXXPXXXXXPXXPPXXXXP 928
P P P P
Sbjct: 1161 VPKPSVAAPPVPAPSSGIP 1179
Score = 30.7 bits (66), Expect = 0.31
Identities = 31/145 (21%), Positives = 31/145 (21%), Gaps = 2/145 (1%)
Frame = +2
Query: 500 PRAXGPPPXXXGXXXPPXXKXPXXXXXXPPPPXXPXXXXXXXXXHXXXP-PPPXXXXPPX 676
P G PP PP P PP P P P P PP
Sbjct: 1077 PAPSGAPPVPAPSGIPPV---PKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPV 1133
Query: 677 XPXXXXXPPPXXXXPXXXFPXPXXXXXXPXXXXXXXXXPXP-XXXXPXXXXXXXXXXXPX 853
P P P P P P P P P
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193
Query: 854 PXPPXPXXXPXXXXXPXXPPXXXXP 928
P P P P PP P
Sbjct: 1194 PSEAPPVPKPSVGVPPVPPPSTAPP 1218
Score = 28.7 bits (61), Expect = 1.2
Identities = 27/138 (19%), Positives = 27/138 (19%)
Frame = +2
Query: 515 PPPXXXGXXXPPXXKXPXXXXXXPPPPXXPXXXXXXXXXHXXXPPPPXXXXPPXXPXXXX 694
PPP P P P P P P P PP
Sbjct: 1063 PPPVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPV--PKPSVAVPPVPAPSGA 1120
Query: 695 XPPPXXXXPXXXFPXPXXXXXXPXXXXXXXXXPXPXXXXPXXXXXXXXXXXPXPXPPXPX 874
P P P P P P P P P P P
Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPP 1180
Query: 875 XXPXXXXXPXXPPXXXXP 928
P PP P
Sbjct: 1181 VPKPAAGVPPVPPPSEAP 1198
Score = 27.5 bits (58), Expect = 2.9
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +1
Query: 838 PPXPPPXPXXPXPXPPXXXXPXXXPXXXXP 927
PP PPP P P P P P P
Sbjct: 1208 PPVPPPSTAPPVPTPSAGLPPVPVPTAKAP 1237
Score = 27.1 bits (57), Expect = 3.8
Identities = 19/81 (23%), Positives = 19/81 (23%)
Frame = +2
Query: 500 PRAXGPPPXXXGXXXPPXXKXPXXXXXXPPPPXXPXXXXXXXXXHXXXPPPPXXXXPPXX 679
P PP PP K PPP P PPP P
Sbjct: 1164 PSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVG--VPPVPPPSTAPPVPT 1221
Query: 680 PXXXXXPPPXXXXPXXXFPXP 742
P P P P P
Sbjct: 1222 PSAGLPPVPVPTAKAPPVPAP 1242
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 30.3 bits (65), Expect = 0.41
Identities = 13/28 (46%), Positives = 13/28 (46%)
Frame = +3
Query: 846 PPXXPXXPPXXPPXXXXPPPXPPXXXPP 929
PP P PP PP PP PP PP
Sbjct: 5 PPGNPPPPP--PPPGFEPPSQPPPPPPP 30
Score = 27.5 bits (58), Expect = 2.9
Identities = 11/26 (42%), Positives = 11/26 (42%)
Frame = +1
Query: 835 PPPXPPPXPXXPXPXPPXXXXPXXXP 912
PP PPP P P PP P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPPPP 30
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 28.7 bits (61), Expect = 1.2
Identities = 12/36 (33%), Positives = 13/36 (36%)
Frame = +3
Query: 501 PGPXXLPXXXGXXXXPPPXXXPXXXXXXPPPPXXPP 608
P P +P PPP P PPP PP
Sbjct: 746 PAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.9 bits (59), Expect = 2.2
Identities = 14/52 (26%), Positives = 14/52 (26%)
Frame = +2
Query: 515 PPPXXXGXXXPPXXKXPXXXXXXPPPPXXPXXXXXXXXXHXXXPPPPXXXXP 670
PPP PP P PPPP P P P P
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
Score = 27.1 bits (57), Expect = 3.8
Identities = 14/50 (28%), Positives = 14/50 (28%)
Frame = +3
Query: 498 PPGPXXLPXXXGXXXXPPPXXXPXXXXXXPPPPXXPPXXXXXXXXTXXPP 647
PP P P P P PPPP PP PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 27.1 bits (57), Expect = 3.8
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +1
Query: 838 PPXPPPXPXXPXPXPPXXXXPXXXPXXXXP 927
PP PPP P P P P P P
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGP 761
Score = 27.1 bits (57), Expect = 3.8
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +1
Query: 835 PPPXPPPXPXXPXPXPPXXXXP 900
PPP PPP P PP P
Sbjct: 762 PPPPPPPGVAGAGPPPPPPPPP 783
Score = 26.2 bits (55), Expect = 6.6
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +1
Query: 796 PPXXXXXXXXXXXPPPXPPPXPXXPXPXPP 885
PP P P PPP P P PP
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPP 764
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 28.3 bits (60), Expect = 1.6
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = +3
Query: 846 PPXXPXXPPXXPPXXXXPPPXPPXXXP 926
PP PP PP PP PP P
Sbjct: 1710 PPMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 27.1 bits (57), Expect = 3.8
Identities = 11/31 (35%), Positives = 11/31 (35%)
Frame = +1
Query: 835 PPPXPPPXPXXPXPXPPXXXXPXXXPXXXXP 927
PPP PP P P PP P P
Sbjct: 1716 PPPSAPPMPAGPPSAPPPPLPASSAPSVPNP 1746
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 25.8 bits (54), Expect = 8.7
Identities = 10/24 (41%), Positives = 10/24 (41%)
Frame = +3
Query: 858 PXXPPXXPPXXXXPPPXPPXXXPP 929
P PP P P P PP PP
Sbjct: 221 PEIPPTYTPKQADPLPAPPPPPPP 244
Score = 23.0 bits (47), Expect(2) = 3.5
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +1
Query: 835 PPPXPPPXP 861
PPP PPP P
Sbjct: 189 PPPPPPPPP 197
Score = 22.2 bits (45), Expect(2) = 3.5
Identities = 9/25 (36%), Positives = 9/25 (36%)
Frame = +1
Query: 838 PPXPPPXPXXPXPXPPXXXXPXXXP 912
PP P P P PP P P
Sbjct: 224 PPTYTPKQADPLPAPPPPPPPTLPP 248
>SPAC23D3.10c |eng2||endo-1,3-beta-glucanase
Eng2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 706
Score = 26.2 bits (55), Expect = 6.6
Identities = 12/29 (41%), Positives = 17/29 (58%), Gaps = 3/29 (10%)
Frame = -3
Query: 287 HCGTSHGPTWS---TKSWTSGFSQSGSAI 210
+ GT H P+WS + +W +G S G AI
Sbjct: 45 YIGTRHNPSWSHPYSVTWLNGSSYYGLAI 73
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.6
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +1
Query: 835 PPPXPPPXPXXPXPXPP 885
PPP P P P P PP
Sbjct: 6 PPPPPAPAPAAAAPAPP 22
>SPBP8B7.26 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 262
Score = 25.8 bits (54), Expect = 8.7
Identities = 11/33 (33%), Positives = 11/33 (33%)
Frame = +3
Query: 498 PPGPXXLPXXXGXXXXPPPXXXPXXXXXXPPPP 596
P P P PPP P PPPP
Sbjct: 123 PSRPGSRPSVLNQEQVPPPPVRPNVMSQMPPPP 155
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 25.8 bits (54), Expect = 8.7
Identities = 15/64 (23%), Positives = 15/64 (23%)
Frame = +2
Query: 515 PPPXXXGXXXPPXXKXPXXXXXXPPPPXXPXXXXXXXXXHXXXPPPPXXXXPPXXPXXXX 694
PPP PP PPPP P P P
Sbjct: 315 PPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPRSAPSTGR 374
Query: 695 XPPP 706
PPP
Sbjct: 375 QPPP 378
Score = 25.8 bits (54), Expect = 8.7
Identities = 18/63 (28%), Positives = 18/63 (28%), Gaps = 2/63 (3%)
Frame = +3
Query: 501 PGPXXLPXXXGXXXXPP-PXXXPXXXXXXPP-PPXXPPXXXXXXXXTXXPPXXPXXXXPP 674
P P LP P P P PP PP P PP P PP
Sbjct: 418 PTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477
Query: 675 XXP 683
P
Sbjct: 478 PAP 480
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,138,763
Number of Sequences: 5004
Number of extensions: 36830
Number of successful extensions: 313
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 84
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 206
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 473333082
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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