Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_P23
(922 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr ... 33 0.043
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 31 0.30
SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription te... 31 0.30
SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.7
SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr 2|||Ma... 27 3.7
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 3.7
SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor Thi1|Sc... 26 6.5
SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase |Schizosa... 26 6.5
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 8.6
>SPAC8E11.10 |||sorbose reductase |Schizosaccharomyces pombe|chr
1|||Manual
Length = 255
Score = 33.5 bits (73), Expect = 0.043
Identities = 18/54 (33%), Positives = 30/54 (55%), Gaps = 2/54 (3%)
Frame = +3
Query: 123 MLAASAGVV--ELSADTSNQDLEEKLYNSILTGDYDSAVRQSLEYESQGKGSII 278
++ A+AG+ LS + N+D+ K+ L G Y +A ++ QGKGS+I
Sbjct: 91 VMIANAGIAIPHLSLEDKNEDIWTKVVGINLNGAYYTAQAAGHHFKKQGKGSLI 144
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 30.7 bits (66), Expect = 0.30
Identities = 19/61 (31%), Positives = 24/61 (39%), Gaps = 1/61 (1%)
Frame = +2
Query: 716 PPSTKXHRPGSSXXNRPIQXCXGTSVXTLXXPPGXPKGPXGPXSSXXPPXP-XXYPVAPX 892
P ST RP S+ + ++ PP P P GP S+ PP P P P
Sbjct: 1686 PVSTPPVRPQSAAPPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAPSVPN 1745
Query: 893 P 895
P
Sbjct: 1746 P 1746
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/30 (43%), Positives = 14/30 (46%)
Frame = +2
Query: 809 PPGXPKGPXGPXSSXXPPXPXXYPVAPXPP 898
PP P P S+ PP P P AP PP
Sbjct: 1707 PPPPPMSVPPPPSA--PPMPAGPPSAPPPP 1734
>SPBC1198.11c |reb1|SPBC660.01c|RNA polymerase I transcription
termination factor Reb1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 504
Score = 30.7 bits (66), Expect = 0.30
Identities = 15/61 (24%), Positives = 31/61 (50%)
Frame = +3
Query: 270 SIIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVRKYFPLNFRLIMAGNYVKIIYRNYNL 449
+II V+N I+D+ + ++C ++W G + +R ++ N ++ K IY +
Sbjct: 247 AIISQEVHNFIMDQGWSEYQFCNQIWAGKCPKTIRMFYS-NLYKKLSHRDAKSIYHHVRR 305
Query: 450 A 452
A
Sbjct: 306 A 306
>SPAC23H3.04 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 300
Score = 27.1 bits (57), Expect = 3.7
Identities = 13/26 (50%), Positives = 18/26 (69%)
Frame = -3
Query: 248 FQALTDSTVVVAGEDAVVQFLLEVLV 171
F +T V+V EDAVV+F+L +LV
Sbjct: 181 FLGVTVQYVMVLPEDAVVEFVLTILV 206
>SPBC27B12.06 |gpi13||pig-O |Schizosaccharomyces pombe|chr
2|||Manual
Length = 918
Score = 27.1 bits (57), Expect = 3.7
Identities = 16/42 (38%), Positives = 25/42 (59%), Gaps = 5/42 (11%)
Frame = -3
Query: 386 WEVLSNNFLS--VADPQL---VAVLHGVPSLVNDQVVNYILD 276
W+VL +++L+ ++ P V LHGV + VN V +YI D
Sbjct: 188 WDVLFHDYLNETLSQPAFSFNVPDLHGVDNKVNQYVFDYIKD 229
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.1 bits (57), Expect = 3.7
Identities = 12/26 (46%), Positives = 18/26 (69%)
Frame = +3
Query: 171 NQDLEEKLYNSILTGDYDSAVRQSLE 248
+ DLE+ + ++LTGD SAV+ LE
Sbjct: 551 DSDLEKNITEALLTGDVLSAVKACLE 576
>SPAC1486.10 |thi1|ntf1, SPAC6G10.01|transcription factor
Thi1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 775
Score = 26.2 bits (55), Expect = 6.5
Identities = 13/37 (35%), Positives = 19/37 (51%)
Frame = +1
Query: 136 ARASLNYPRTLLTKTSRRNCTTASSPATTTVLSVRAW 246
AR SLNY ++ + SRRN ++P S + W
Sbjct: 622 ARESLNYLKSFNKQLSRRNAPDINNPIADFQNSFQNW 658
>SPBC577.13 |syj2||inositol-polyphosphate 5-phosphatase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 889
Score = 26.2 bits (55), Expect = 6.5
Identities = 11/34 (32%), Positives = 18/34 (52%)
Frame = +3
Query: 273 IIQNVVNNLIIDKRRNTMEYCYKLWVGNGQEIVR 374
+++++ NL I N +EY LW NG I +
Sbjct: 447 VLKDIFFNLQIGVTFNILEYLRHLWSNNGDAIAK 480
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 25.8 bits (54), Expect = 8.6
Identities = 13/31 (41%), Positives = 13/31 (41%), Gaps = 2/31 (6%)
Frame = +3
Query: 822 PKAPX--GPXPPXXPPXXXXTXWPPXPLXKP 908
P AP GP PP PP PP P P
Sbjct: 753 PPAPIMGGPPPPPPPPGVAGAGPPPPPPPPP 783
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,078,550
Number of Sequences: 5004
Number of extensions: 56141
Number of successful extensions: 154
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 135
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 151
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 468512460
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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