BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_P19
(949 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 32 0.14
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.96
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 28 1.7
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 27 5.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.7
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 26 8.9
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 31.9 bits (69), Expect = 0.14
Identities = 14/31 (45%), Positives = 14/31 (45%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPPRXPXAPXPP 568
PP PPP P S G PP P A PP
Sbjct: 1208 PPVPPPSTAPPVPTPSAGLPPVPVPTAKAPP 1238
Score = 28.7 bits (61), Expect = 1.3
Identities = 14/34 (41%), Positives = 14/34 (41%), Gaps = 4/34 (11%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXG----PPPPRXPXAPXP 565
PP PPP P S G PPP P P P
Sbjct: 1189 PPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTP 1222
Score = 27.9 bits (59), Expect = 2.2
Identities = 15/56 (26%), Positives = 17/56 (30%)
Frame = +1
Query: 427 PRPRGXGXXPSXPXXXXSXPXPGXXXXXXGXXXRSPPPPAXXXAXPPXXGQAGLPP 594
P P G P P P +PP P A PP +G PP
Sbjct: 1086 PAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSGAPP 1141
Score = 27.1 bits (57), Expect = 3.9
Identities = 12/39 (30%), Positives = 13/39 (33%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPPRXPXAPXPPXRGKRASP 592
PP P P P G PP P PP +P
Sbjct: 1121 PPVPKPSVAAPPVPVPSGAPPVPKPSVAAPPVPAPSGAP 1159
Score = 26.6 bits (56), Expect = 5.1
Identities = 16/58 (27%), Positives = 19/58 (32%), Gaps = 2/58 (3%)
Frame = +1
Query: 427 PRPRGXGXXPSXPXXXXSXPXPGXXXXXX--GXXXRSPPPPAXXXAXPPXXGQAGLPP 594
P P PS P + P P +PP P A PP +G PP
Sbjct: 1065 PVPAPSSEIPSIPAPSGAPPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPP 1122
Score = 26.6 bits (56), Expect = 5.1
Identities = 12/39 (30%), Positives = 13/39 (33%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPPRXPXAPXPPXRGKRASP 592
PP P P P S PP P PP +P
Sbjct: 1083 PPVPAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAP 1121
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/57 (28%), Positives = 18/57 (31%), Gaps = 1/57 (1%)
Frame = +1
Query: 427 PRPRGXGXXPSXPXXXXSXPXPGXXXXXXGXXXRSPPPPAXXXAXPP-XXGQAGLPP 594
P P G P P P +PP PA PP AG+PP
Sbjct: 1134 PVPSGAPPVPKPSVAAPPVPAPSGAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPP 1190
Score = 25.8 bits (54), Expect = 8.9
Identities = 11/31 (35%), Positives = 11/31 (35%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPPRXPXAPXPP 568
PP P P P G PP P PP
Sbjct: 1140 PPVPKPSVAAPPVPAPSGAPPVPKPSVAAPP 1170
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.1 bits (62), Expect = 0.96
Identities = 12/31 (38%), Positives = 13/31 (41%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPPRXPXAPXPP 568
PP PPP P + P PP P PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPP 762
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/22 (45%), Positives = 10/22 (45%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPP 541
PP PPP G PPPP
Sbjct: 761 PPPPPPPPGVAGAGPPPPPPPP 782
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 28.3 bits (60), Expect = 1.7
Identities = 12/31 (38%), Positives = 12/31 (38%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPPPPRXPXAPXPP 568
PP PP P PP P AP PP
Sbjct: 447 PPLPPSAPIAPPLPAGMPAAPPLPPAAPAPP 477
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 26.6 bits (56), Expect = 5.1
Identities = 14/42 (33%), Positives = 15/42 (35%), Gaps = 1/42 (2%)
Frame = +2
Query: 470 TXPPXPPPXXXXPXXGXSXGPPPPRXPXAPXPP-XRGKRASP 592
T PP PP PPPP+ PP G R P
Sbjct: 906 TLPPPPPTASMTASAPAIASPPPPKVGETYHPPTASGTRVPP 947
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/30 (40%), Positives = 12/30 (40%)
Frame = +2
Query: 479 PXPPPXXXXPXXGXSXGPPPPRXPXAPXPP 568
P PPP S P P P AP PP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 26.2 bits (55), Expect = 6.7
Identities = 13/37 (35%), Positives = 15/37 (40%)
Frame = +2
Query: 479 PXPPPXXXXPXXGXSXGPPPPRXPXAPXPPXRGKRAS 589
P PP P S PPP AP P G R++
Sbjct: 1715 PPPPSAPPMPAGPPSAPPPPLPASSAPSVPNPGDRSA 1751
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/47 (34%), Positives = 19/47 (40%), Gaps = 8/47 (17%)
Frame = +2
Query: 476 PPXPPPXXXXPXXGXSXGPP--------PPRXPXAPXPPXRGKRASP 592
PP PPP P S PP PP+ AP PP + +P
Sbjct: 206 PPPPPPQQNYPPAASSSAPPMQYQQTAYPPQ--QAPYPPVQAYPQAP 250
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.313 0.140 0.466
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,505,277
Number of Sequences: 5004
Number of extensions: 16223
Number of successful extensions: 109
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 30
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 90
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 483319012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 42 (21.8 bits)
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