Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_M19
(828 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 285 4e-78
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 281 6e-77
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 149 6e-37
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 118 1e-27
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.5
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.5
SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces pom... 25 9.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 285 bits (700), Expect = 4e-78
Identities = 127/203 (62%), Positives = 155/203 (76%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 506 RIRKLADQCTGLQXFLIXXXXXXXXXXXXXXLLMERLSVDYGXXSXLEFXIYPXPXVSLA 685
+IR++AD C+GLQ FL+ LL+ERL+++Y S L+F +YP P VS +
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTS 180
Query: 686 VVEPYNSILXTHTTLEXSDXAFM 754
VVEPYNS+L TH TL+ +D FM
Sbjct: 181 VVEPYNSVLTTHATLDLADCTFM 203
Score = 26.6 bits (56), Expect = 4.3
Identities = 10/13 (76%), Positives = 11/13 (84%)
Frame = +3
Query: 756 VDXEAIYDICRRN 794
VD E+ YDICRRN
Sbjct: 204 VDNESCYDICRRN 216
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 281 bits (690), Expect = 6e-77
Identities = 130/208 (62%), Positives = 158/208 (75%), Gaps = 5/208 (2%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 310
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 311 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 490
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 491 DLVLDRIRKLADQCTGLQXFLIXXXXXXXXXXXXXXLLMERLSVDYGXXSXLEFXIYPXP 670
D VL+RIR++AD C+GLQ FL+ LL+ERL+++YG S L+F +YP P
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAP 179
Query: 671 XVSLAVVEPYNSILXTHTTLEXSDXAFM 754
VS +VVEPYNS+L TH TL+ SD FM
Sbjct: 180 QVSTSVVEPYNSVLTTHATLDNSDCTFM 207
Score = 27.9 bits (59), Expect = 1.9
Identities = 11/13 (84%), Positives = 11/13 (84%)
Frame = +3
Query: 756 VDXEAIYDICRRN 794
VD EA YDICRRN
Sbjct: 208 VDNEACYDICRRN 220
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 149 bits (360), Expect = 6e-37
Identities = 76/202 (37%), Positives = 105/202 (51%)
Frame = +2
Query: 146 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 325
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 326 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 505
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 506 RIRKLADQCTGLQXFLIXXXXXXXXXXXXXXLLMERLSVDYGXXSXLEFXIYPXPXVSLA 685
+R+ A+ C LQ F + LL+ ++ +Y F + P P S
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEYPDRMMATFSVAPAPKSSDT 178
Query: 686 VVEPYNSILXTHTTLEXSDXAF 751
VVEPYN+ L H +E SD F
Sbjct: 179 VVEPYNATLSMHQLVENSDETF 200
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 118 bits (284), Expect = 1e-27
Identities = 67/191 (35%), Positives = 104/191 (54%), Gaps = 3/191 (1%)
Frame = +2
Query: 149 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 328
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 329 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 502
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 503 DRIRKLADQCTGLQXFLIXXXXXXXXXXXXXXLLMERLSVDYGXXSXLEFXIYP-XPXVS 679
D I + AD L+ F + L+ERL+ Y + ++P VS
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKKIIQTYSVFPNSQSVS 179
Query: 680 LAVVEPYNSIL 712
VV+PYNS+L
Sbjct: 180 DVVVQPYNSLL 190
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.5
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +3
Query: 225 STASSLMARCPQTRPSGVETILSTLSSARPELAST 329
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.5
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +1
Query: 244 WPDAHRQDHRGWRRFFQHFLQR 309
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
>SPCC16A11.08 |atg20||sorting nexin Atg20|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 534
Score = 25.4 bits (53), Expect = 9.9
Identities = 15/41 (36%), Positives = 21/41 (51%)
Frame = +3
Query: 135 LKSKCVSASLYTLAKPESRSVMPAGSFTAWSTASSLMARCP 257
L+S +L+ P SR++ P S + STASSL P
Sbjct: 170 LRSSMPLVMANSLSPPSSRALKPIHSLSNPSTASSLEPSSP 210
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,143,979
Number of Sequences: 5004
Number of extensions: 61916
Number of successful extensions: 164
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 151
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 159
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 406444570
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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