Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_L20
(860 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 36 0.007
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 35 0.013
SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain pro... 31 0.21
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 31 0.28
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.37
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.49
SPAC4F10.08 |mug126||sequence orphan|Schizosaccharomyces pombe|c... 29 0.64
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 29 0.85
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.1
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 1.5
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 2.0
SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr... 28 2.0
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 2.0
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.6
SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyce... 27 3.4
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 4.5
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 4.5
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 4.5
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.0
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 7.9
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 7.9
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 35.9 bits (79), Expect = 0.007
Identities = 32/99 (32%), Positives = 52/99 (52%), Gaps = 4/99 (4%)
Frame = +2
Query: 200 IEHHTKEFHKTLEQQFNSLTKSKDAQDF---SKAWKDGSESVLQQLNAFAKSLQGALGDA 370
I+ K LEQ+ +L ++++A++ ++ + D S S +L A AK A DA
Sbjct: 98 IQPDEKTLQDLLEQRQVALREAREAEEELQRARQYNDRSTSEALELEARAKK---AAQDA 154
Query: 371 NGKAKEALEQSRQNIERTAEELRK-AHPDVEKNATALRE 484
A E +++ +IER+A K A + E+ ATALRE
Sbjct: 155 E-LASERAREAQSSIERSASLREKQAREEAERAATALRE 192
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 35.1 bits (77), Expect = 0.013
Identities = 28/107 (26%), Positives = 55/107 (51%), Gaps = 6/107 (5%)
Frame = +2
Query: 230 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 400
T+E ++SL KSK + F + ++ + +L N + + L + +A KA+++LEQ
Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65
Query: 401 SRQNIERTAEELRKAHPD---VEKNATALREKLQAAVQNTVQESQKL 532
+ + E L K H + E+ + +EKL A ++ + S++L
Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDAMLKRKQKLSEEL 112
>SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 240
Score = 31.1 bits (67), Expect = 0.21
Identities = 20/77 (25%), Positives = 40/77 (51%), Gaps = 1/77 (1%)
Frame = +2
Query: 386 EALEQSRQNIE-RTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNVQE 562
+ E++ + I+ R+ EEL++A + + A +EK + + + K+ +K + +
Sbjct: 28 DQFEETEEEIKKRSPEELKQAIEALREKAKEKKEKERILALEEKKTNYKILQKSNDETAQ 87
Query: 563 TNEKLAPQDQGRLRRLR 613
K+ QDQ RLR L+
Sbjct: 88 AMRKM--QDQARLRDLQ 102
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 30.7 bits (66), Expect = 0.28
Identities = 30/131 (22%), Positives = 53/131 (40%)
Frame = +2
Query: 83 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 262
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 263 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRK 442
SKDA A S+ + S + +L N K++ +L+ ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSRNIIRRVF 288
Query: 443 AHPDVEKNATA 475
P+V + +A
Sbjct: 289 HKPEVHLDPSA 299
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 30.3 bits (65), Expect = 0.37
Identities = 17/81 (20%), Positives = 38/81 (46%)
Frame = +2
Query: 329 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 508
N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + +
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396
Query: 509 TVQESQKLAKKVSSNVQETNE 571
+QE ++ K + Q+ +E
Sbjct: 397 IIQEKSRVDKMIDEYRQKLSE 417
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.49
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +2
Query: 296 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 433
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPAC4F10.08 |mug126||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 436
Score = 29.5 bits (63), Expect = 0.64
Identities = 16/52 (30%), Positives = 28/52 (53%), Gaps = 2/52 (3%)
Frame = +2
Query: 440 KAHPDVEKNATALREKLQAAVQN--TVQESQKLAKKVSSNVQETNEKLAPQD 589
++ D +K +T E+LQ A + TVQE+ + KV+ + +K PQ+
Sbjct: 157 ESQTDKKKTSTVQEEELQNAGKKLETVQENPQAYSKVTQQEPQAGQKAVPQE 208
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 29.1 bits (62), Expect = 0.85
Identities = 17/69 (24%), Positives = 38/69 (55%)
Frame = +2
Query: 377 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSNV 556
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+S N+
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-----EKISRNL 185
Query: 557 QETNEKLAP 583
+ ++ ++P
Sbjct: 186 RSSSRSISP 194
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.1
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +2
Query: 275 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 430
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.3 bits (60), Expect = 1.5
Identities = 24/88 (27%), Positives = 45/88 (51%)
Frame = +2
Query: 200 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 379
IE K F K ++ NS K+ +A F +G++ ++ + A ++L L +
Sbjct: 73 IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125
Query: 380 AKEALEQSRQNIERTAEELRKAHPDVEK 463
+EQ+R E T E++++A P++EK
Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 2.0
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +2
Query: 170 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 340
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 341 KSLQGALGDA 370
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPCC364.04c |||CASP family protein|Schizosaccharomyces pombe|chr
3|||Manual
Length = 633
Score = 27.9 bits (59), Expect = 2.0
Identities = 19/82 (23%), Positives = 37/82 (45%), Gaps = 3/82 (3%)
Frame = +2
Query: 377 KAKEALE---QSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVS 547
K KE +E Q ++ +ER E LRK + EK A + ++S +K+
Sbjct: 128 KEKEEMEGSLQGKEKLEREVENLRKELDKYKDLVETEAEKRAAITKEECEKSWLEQQKLY 187
Query: 548 SNVQETNEKLAPQDQGRLRRLR 613
++++ N + ++R L+
Sbjct: 188 KDMEQENASTIQKLTSKIRELQ 209
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 2.0
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +2
Query: 230 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 391
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 392 LEQSRQNIERTAEE 433
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.6
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 407 ASTVPKPPWPCRSRLRALPG 348
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPBC6B1.09c |nbs1||Mre11 complex subunit Nbs1|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 613
Score = 27.1 bits (57), Expect = 3.4
Identities = 22/89 (24%), Positives = 41/89 (46%), Gaps = 6/89 (6%)
Frame = +2
Query: 362 GDANGKAKEALEQSRQNIERTAEELRK------AHPDVEKNATALREKLQAAVQNTVQES 523
GD + K K +L+ S + T K ++ V+K ++ + L+ V + +
Sbjct: 402 GDNSEKTKNSLKSSSKKSANTGSGQGKTKVEYVSYNSVDKGNSSPFKPLELNVVGEKKAN 461
Query: 524 QKLAKKVSSNVQETNEKLAPQDQGRLRRL 610
++ S NVQE+ + A ++ RLR L
Sbjct: 462 AEVDSLPSENVQESEDDKAFEENRRLRNL 490
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 4.5
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +2
Query: 176 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 295
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.6 bits (56), Expect = 4.5
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +2
Query: 344 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 499
S++ L + N + KE +E + RT +E EKN LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 26.6 bits (56), Expect = 4.5
Identities = 22/74 (29%), Positives = 32/74 (43%)
Frame = +2
Query: 305 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 484
S + + A K L GA KAKE ++ R +RTA E+RK +E+ R
Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199
Query: 485 KLQAAVQNTVQESQ 526
+ A Q + Q
Sbjct: 200 ERAAEAQRVAGKEQ 213
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.0
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +2
Query: 212 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 370
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 7.9
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +2
Query: 140 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 316
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 317 LQQL 328
Q+
Sbjct: 73 NNQI 76
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.8 bits (54), Expect = 7.9
Identities = 18/77 (23%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +2
Query: 224 HKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANG---KAKEAL 394
H+T+ +Q + +A + ES L N ++ L ++N K +E +
Sbjct: 625 HQTITKQLKDTSSKLQQLQLERANFEQKESTLSDENNDLRTKLLKLEESNKSLIKKQEDV 684
Query: 395 EQSRQNIERTAEELRKA 445
+ +NI+ E+LRK+
Sbjct: 685 DSLEKNIQTLKEDLRKS 701
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,320,221
Number of Sequences: 5004
Number of extensions: 35764
Number of successful extensions: 185
Number of sequences better than 10.0: 21
Number of HSP's better than 10.0 without gapping: 175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 184
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 428468660
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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