BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_L04
(853 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr... 28 1.5
SPCC1739.11c |cdc11||SIN component scaffold protein Cdc11|Schizo... 28 1.9
SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor |S... 27 2.6
SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr... 27 2.6
SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein |S... 27 3.4
SPBC13G1.10c |mug81||ATP-dependent RNA helicase Slh1|Schizosacch... 27 4.5
SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyce... 27 4.5
SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3 |Schi... 26 5.9
SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces pombe... 26 5.9
>SPCC1919.10c |myo52||myosin type V|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1516
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/52 (30%), Positives = 30/52 (57%)
Frame = +2
Query: 467 DLKRVLDKEVNVIDNGFMFYFMKQIQEATLEHEQVKKLLEDPNKXFDIVIVE 622
+LK +L+++ N + M M+Q+ +A E +V L+ K +D++IVE
Sbjct: 989 ELKELLEEKENALI--MMEEEMRQVNDANTELLRVNATLKSQLKNYDMIIVE 1038
>SPCC1739.11c |cdc11||SIN component scaffold protein
Cdc11|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1045
Score = 27.9 bits (59), Expect = 1.9
Identities = 13/53 (24%), Positives = 29/53 (54%)
Frame = -1
Query: 688 RQPYKWNVEYRSITSTQFAIHPLNDNNIEXLIRVLEQLFNLLMLQSSFLYLFH 530
+QP+ + + +++ ++F H L DN+ E ++L +L + SF +F+
Sbjct: 500 QQPHSFPLRKQNVAQSEFPKHSLRDNS-ENAPQILSSFHDLSLQNESFDEMFN 551
>SPAC1851.04c ||SPAC27D7.01c|guanyl-nucleotide exchange factor
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1052
Score = 27.5 bits (58), Expect = 2.6
Identities = 18/56 (32%), Positives = 25/56 (44%), Gaps = 3/56 (5%)
Frame = +2
Query: 539 IQEATLEHEQVKKLLEDPNKXFDIVIVEWMYCELGASYAAVFDVPL---IWLSTMG 697
IQ +TLE KL + +K +D+ V W FDV + W+ST G
Sbjct: 155 IQVSTLETTDPNKLWKVSSKQYDLREVTWFLDHSSLISRLYFDVKIDTFFWMSTDG 210
>SPAC16E8.09 |scd1|ral1|RhoGEF Scd1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 872
Score = 27.5 bits (58), Expect = 2.6
Identities = 18/66 (27%), Positives = 30/66 (45%), Gaps = 1/66 (1%)
Frame = +2
Query: 473 KRVLDKEVNVIDNGFMFYFMKQIQEATLEHEQVKKLLEDPNKXFDIVIVE-WMYCELGAS 649
+R L+ ++D + F I L K+LE + FD + E W++C LG
Sbjct: 62 RRCLNLRKRLMDISELAAFFDSIHREALNSSF--KILEFKDIEFDDPVTEIWLFCRLGYP 119
Query: 650 YAAVFD 667
A+F+
Sbjct: 120 LCALFN 125
>SPAC589.12 ||SPAC688.01|glycosylceramide biosynthesis protein
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 971
Score = 27.1 bits (57), Expect = 3.4
Identities = 12/30 (40%), Positives = 16/30 (53%)
Frame = -1
Query: 577 LFNLLMLQSSFLYLFHEIKHKPVVYYVYLF 488
LF L + + Y+ H+ KH P Y VY F
Sbjct: 168 LFVLTIFPLIYWYIQHKFKHIPGAYTVYAF 197
>SPBC13G1.10c |mug81||ATP-dependent RNA helicase
Slh1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1935
Score = 26.6 bits (56), Expect = 4.5
Identities = 18/82 (21%), Positives = 34/82 (41%), Gaps = 4/82 (4%)
Frame = -2
Query: 768 RHRIPVVGLDLDSHR*ASSPTSEAPMVDNHISGTSNTAA*LA----PNSQYIHSTITISK 601
RH ++ ++++ S+ PMVD H+ T A +A P Y+ T T+
Sbjct: 1667 RHNEDLINIEINKSLKYSAACLNLPMVDAHVKAFILTQAHMARLKLPVDDYVTDTSTVLD 1726
Query: 600 XLFGSSSNFLTCSCSRVASCIC 535
+ +++ S S +C
Sbjct: 1727 QVIRIIQSYIDVSAELGYSHVC 1748
>SPBC27B12.04c |||conserved eukaryotic protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 817
Score = 26.6 bits (56), Expect = 4.5
Identities = 15/58 (25%), Positives = 29/58 (50%)
Frame = -1
Query: 667 VEYRSITSTQFAIHPLNDNNIEXLIRVLEQLFNLLMLQSSFLYLFHEIKHKPVVYYVY 494
+EY +T FAI ++I L VL L +L ++ + L H ++ + + + +Y
Sbjct: 519 IEYTEDFATGFAISGEISHSINNLEFVLYSLSSLFLMMLKWFRLSHVLRFERLAFLLY 576
>SPBC26H8.10 |dis3|rrp44|3'-5' exoribonuclease subunit Dis3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 970
Score = 26.2 bits (55), Expect = 5.9
Identities = 8/29 (27%), Positives = 19/29 (65%)
Frame = +2
Query: 581 LEDPNKXFDIVIVEWMYCELGASYAAVFD 667
LE PN FD++I++ ++ E+ + +++
Sbjct: 99 LEHPNNFFDVIILQTVFSEISSKSIPLYN 127
>SPBC106.14c |sda1||SDA1 family protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 719
Score = 26.2 bits (55), Expect = 5.9
Identities = 11/42 (26%), Positives = 19/42 (45%)
Frame = -2
Query: 561 CSRVASCICFMK*NINPLSITFTSLSRTLFKSNPPLGIKRIL 436
C ++ C+ +K IT LF+ NP G++ +L
Sbjct: 98 CEKIVLCLVLLKNKTVISPITLLQCFFPLFRENPTRGVRELL 139
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,519,758
Number of Sequences: 5004
Number of extensions: 75971
Number of successful extensions: 197
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 193
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 197
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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