Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_K07
(857 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar... 254 2e-68
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch... 246 2e-66
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces... 127 2e-30
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|... 114 2e-26
SPAC8E11.03c |dmc1|dmp1|RecA family ATPase Dmc1|Schizosaccharomy... 28 1.5
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||... 26 7.9
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr... 26 7.9
>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
2|Schizosaccharomyces pombe|chr 2|||Manual
Length = 449
Score = 254 bits (621), Expect = 2e-68
Identities = 112/167 (67%), Positives = 134/167 (80%)
Frame = +1
Query: 154 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 333
MRE IS+HVGQAG QIGNACWELYCLEHGIQP+G M + D F+TFFSETG GK
Sbjct: 1 MREIISIHVGQAGTQIGNACWELYCLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGK 60
Query: 334 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 513
+VPR+++VDLEP V+D+VRTG YR LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D
Sbjct: 61 YVPRSIYVDLEPNVIDQVRTGPYRDLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTD 120
Query: 514 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKL 654
+IR++AD C+GLQGFL+FH LL+ERL+++Y KKSKL
Sbjct: 121 KIRRIADNCSGLQGFLVFHSFGGGTGSGFGALLLERLAMEYTKKSKL 167
Score = 56.0 bits (129), Expect = 6e-09
Identities = 24/40 (60%), Positives = 29/40 (72%)
Frame = +3
Query: 672 PRLRFPLAVVEPYNSILTXHTTLEHSDCAFMVDNEXIYDI 791
P + +VVEPYNS+LT H TL+ +DC FMVDNE YDI
Sbjct: 173 PAPQVSTSVVEPYNSVLTTHATLDLADCTFMVDNESCYDI 212
Score = 26.2 bits (55), Expect = 6.0
Identities = 9/12 (75%), Positives = 12/12 (100%)
Frame = +2
Query: 656 EFAIYPAPQVST 691
+F++YPAPQVST
Sbjct: 168 QFSVYPAPQVST 179
>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
2|||Manual
Length = 455
Score = 246 bits (603), Expect = 2e-66
Identities = 113/172 (65%), Positives = 136/172 (79%), Gaps = 5/172 (2%)
Frame = +1
Query: 154 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSE 318
MRE ISVHVGQAGVQIGNACWELYCLEHGI PDG PT+ K +D F TFFSE
Sbjct: 1 MREVISVHVGQAGVQIGNACWELYCLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSE 59
Query: 319 TGAGKHVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIV 498
TG GK VPR+++VDLEP V+D+VRTG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++
Sbjct: 60 TGQGKFVPRSIYVDLEPNVIDQVRTGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMI 119
Query: 499 DLVLDRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKKSKL 654
D VL+RIR++AD C+GLQGFL+FH LL+ERL+++YGKKS L
Sbjct: 120 DSVLERIRRMADNCSGLQGFLVFHSFGGGTGSGLGALLLERLNMEYGKKSNL 171
Score = 58.8 bits (136), Expect = 9e-10
Identities = 25/40 (62%), Positives = 30/40 (75%)
Frame = +3
Query: 672 PRLRFPLAVVEPYNSILTXHTTLEHSDCAFMVDNEXIYDI 791
P + +VVEPYNS+LT H TL++SDC FMVDNE YDI
Sbjct: 177 PAPQVSTSVVEPYNSVLTTHATLDNSDCTFMVDNEACYDI 216
Score = 27.9 bits (59), Expect = 2.0
Identities = 10/14 (71%), Positives = 13/14 (92%)
Frame = +2
Query: 650 NWEFAIYPAPQVST 691
N +F++YPAPQVST
Sbjct: 170 NLQFSVYPAPQVST 183
>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 448
Score = 127 bits (306), Expect = 2e-30
Identities = 62/161 (38%), Positives = 88/161 (54%)
Frame = +1
Query: 154 MRECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGK 333
MRE + + GQ G Q+G A W EHG+ G T + N +F+E GK
Sbjct: 1 MREIVHIQAGQCGNQVGAAFWSTIADEHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGK 58
Query: 334 HVPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLD 513
+VPRAV VDLEP +D V++G + LF P+ +I G+ A N +A+GHYT G E+ D VLD
Sbjct: 59 YVPRAVLVDLEPGTMDAVKSGKFGNLFRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLD 118
Query: 514 RIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDY 636
+R+ A+ C LQGF + H LL+ ++ +Y
Sbjct: 119 VVRREAEACDALQGFQLTHSLGGGTGSGMGTLLLSKIREEY 159
Score = 38.3 bits (85), Expect = 0.001
Identities = 16/33 (48%), Positives = 23/33 (69%)
Frame = +3
Query: 696 VVEPYNSILTXHTTLEHSDCAFMVDNEXIYDIW 794
VVEPYN+ L+ H +E+SD F +DNE + I+
Sbjct: 179 VVEPYNATLSMHQLVENSDETFCIDNEALSSIF 211
>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 446
Score = 114 bits (274), Expect = 2e-26
Identities = 61/165 (36%), Positives = 93/165 (56%), Gaps = 2/165 (1%)
Frame = +1
Query: 157 RECISVHVGQAGVQIGNACWELYCLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKH 336
RE I++ GQ G QIG+ W+ CLEHGI PDG + + T G D + FF ++ ++
Sbjct: 3 REIITLQAGQCGNQIGSQFWQQLCLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRY 60
Query: 337 VPRAVFVDLEPTVVDEVRTGTYRQLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVL 510
+PRA+ +DLEP VV+ + + TY L++PE ++ K A NN+A G Y+ + I + ++
Sbjct: 61 IPRAILIDLEPRVVNNILSDTYGSLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIM 119
Query: 511 DRIRKLADQCTGLQGFLIFHXXXXXXXXXXXXLLMERLSVDYGKK 645
D I + AD L+GF + H L+ERL+ Y KK
Sbjct: 120 DMIDREADGSDSLEGFSLLHSIAGGTGSGLGSFLLERLNDRYPKK 164
>SPAC8E11.03c |dmc1|dmp1|RecA family ATPase Dmc1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 332
Score = 28.3 bits (60), Expect = 1.5
Identities = 14/37 (37%), Positives = 20/37 (54%)
Frame = -1
Query: 785 IDGFIVDHESTVRVLKGCVXGEDRVVGLDDGQWKPEA 675
+ G ++ H S R+L GE+RV L+D PEA
Sbjct: 281 VGGHVMAHASATRLLLRKGRGEERVAKLNDSPDMPEA 317
>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 534
Score = 25.8 bits (54), Expect = 7.9
Identities = 15/35 (42%), Positives = 19/35 (54%)
Frame = +2
Query: 233 STASSLMARCPQTRPSGVETILSTLSSARPELAST 337
ST SSL + ++PS T ST SSA P S+
Sbjct: 173 STFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207
>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1275
Score = 25.8 bits (54), Expect = 7.9
Identities = 13/22 (59%), Positives = 13/22 (59%)
Frame = +3
Query: 252 WPDAHRQDHRGWRRFFQHFLQR 317
W A R D R R FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,380,618
Number of Sequences: 5004
Number of extensions: 70551
Number of successful extensions: 189
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 171
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 185
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 426466470
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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