Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_J03
(874 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 32 0.12
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.49
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.86
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 29 0.86
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 29 1.1
SPCC895.08c |||conserved fungal protein|Schizosaccharomyces pomb... 28 2.0
SPAC105.01c |||potassium ion/proton antiporter|Schizosaccharomyc... 27 4.6
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 27 4.6
SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit... 26 6.1
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 26 8.1
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 31.9 bits (69), Expect = 0.12
Identities = 20/65 (30%), Positives = 22/65 (33%)
Frame = +3
Query: 444 PXXPYFXPXXSPXKGAPPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSP 623
P P P P APP P P P + P PP P GP +P
Sbjct: 1683 PAHPVSTPPVRPQSAAPPQ------MSAPTPPPPPMSVPPPPSAPPMP-----AGPPSAP 1731
Query: 624 PPXXP 638
PP P
Sbjct: 1732 PPPLP 1736
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.9 bits (64), Expect = 0.49
Identities = 17/54 (31%), Positives = 19/54 (35%)
Frame = +3
Query: 477 PXKGAPPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSPPPXXP 638
P APP P L P P + P PP P + P PPP P
Sbjct: 125 PSAPAPPTPQSELRPPTSAPPRPSIPPPSPASAPPIPSKAPPI-PSSLPPPAQP 177
Score = 29.5 bits (63), Expect = 0.65
Identities = 18/57 (31%), Positives = 20/57 (35%), Gaps = 2/57 (3%)
Frame = +3
Query: 465 PXXSPXKGAPPXPPXI--L*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSPPP 629
P SP APP P + PPP P P P P + P PPP
Sbjct: 150 PPPSPAS-APPIPSKAPPIPSSLPPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPPP 205
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.1 bits (62), Expect = 0.86
Identities = 20/69 (28%), Positives = 26/69 (37%)
Frame = +3
Query: 465 PXXSPXKGAPPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSPPPXXPFL 644
P S G+ P PP + PPP P+ PP + + P +PPP P
Sbjct: 342 PPRSNAAGSIPLPPQG--RSAPPPPPPRSAPSTGRQPPPLSSSRAVSNPP-APPPAIPGR 398
Query: 645 EXFXNPPXG 671
PP G
Sbjct: 399 SAPALPPLG 407
Score = 28.7 bits (61), Expect = 1.1
Identities = 17/50 (34%), Positives = 17/50 (34%)
Frame = +3
Query: 489 APPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSPPPXXP 638
APP PP I PP P L P PP P P P P
Sbjct: 229 APPIPPSIP-SSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPPRPIAP 277
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/77 (27%), Positives = 25/77 (32%), Gaps = 2/77 (2%)
Frame = +3
Query: 453 PYFXPXXSPXKGAPPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSP--P 626
P P P + AP PP + P P P P P + P G+P P
Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRTSTPPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAP 447
Query: 627 PXXPFLEXFXNPPXGXP 677
P P P G P
Sbjct: 448 PLPPSAPIAPPLPAGMP 464
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 29.1 bits (62), Expect = 0.86
Identities = 20/62 (32%), Positives = 22/62 (35%), Gaps = 1/62 (1%)
Frame = +3
Query: 522 KXPPPYSPQLGXXXPXLTP-PXPXTXSXLGPXGSPPPXXPFLEXFXNPPXGXPXXXXFXG 698
K PPP P + P P P P +G G PPP P PP P
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMG--GPPPPPPPPGVAGAGPPPPPPPPPAVSA 787
Query: 699 GG 704
GG
Sbjct: 788 GG 789
Score = 28.7 bits (61), Expect = 1.1
Identities = 16/53 (30%), Positives = 21/53 (39%), Gaps = 1/53 (1%)
Frame = +3
Query: 483 KGAPPXPPXIL*KKXPPPYSPQLGXXXPXLT-PPXPXTXSXLGPXGSPPPXXP 638
K PP PP ++ P P + P + PP P + G PPP P
Sbjct: 730 KSPPPPPPAVI-VPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 28.3 bits (60), Expect = 1.5
Identities = 16/55 (29%), Positives = 19/55 (34%)
Frame = +3
Query: 444 PXXPYFXPXXSPXKGAPPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLG 608
P P +P P P I+ PPP P + P PP P S G
Sbjct: 735 PPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPAVSAGG 789
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 28.7 bits (61), Expect = 1.1
Identities = 13/30 (43%), Positives = 15/30 (50%)
Frame = -3
Query: 665 GGVXKXFQKXGXWGGGPXGAQXGXGXGGGG 576
GG G +GGGP G + G G GGG
Sbjct: 214 GGEGHHHGGHGGFGGGPGGFEGGPGGFGGG 243
Score = 26.6 bits (56), Expect = 4.6
Identities = 11/20 (55%), Positives = 12/20 (60%)
Frame = -3
Query: 635 GXWGGGPXGAQXGXGXGGGG 576
G +GGGP G G G GGG
Sbjct: 238 GGFGGGPGGFGGGLGGFGGG 257
Score = 26.2 bits (55), Expect = 6.1
Identities = 11/21 (52%), Positives = 12/21 (57%)
Frame = -3
Query: 635 GXWGGGPXGAQXGXGXGGGGG 573
G +GGGP G G G GG G
Sbjct: 252 GGFGGGPGGFGGGPGGHGGPG 272
>SPCC895.08c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 490
Score = 27.9 bits (59), Expect = 2.0
Identities = 15/31 (48%), Positives = 18/31 (58%)
Frame = -2
Query: 267 HFNSGALPTLNXVYFTSISIFSTKYXFSTCF 175
H GA LN FTS+S+ S+KY F T F
Sbjct: 149 HIRYGA--PLNDACFTSLSMKSSKYSFLTAF 177
>SPAC105.01c |||potassium ion/proton antiporter|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 898
Score = 26.6 bits (56), Expect = 4.6
Identities = 13/28 (46%), Positives = 19/28 (67%)
Frame = +2
Query: 107 FVLFIAAAVSADDEPVLARLLVSKQVLN 190
F+LFI+ A+S PVLAR+L +L+
Sbjct: 159 FLLFISTAMSITAFPVLARILSELHLLH 186
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.6 bits (56), Expect = 4.6
Identities = 17/46 (36%), Positives = 19/46 (41%)
Frame = +3
Query: 492 PPXPPXIL*KKXPPPYSPQLGXXXPXLTPPXPXTXSXLGPXGSPPP 629
PP PP L K PP +P P PP T + G G P P
Sbjct: 1883 PPPPPMALPKAGPPSAAPT--SALPPAGPPAGAT-AISGNPGMPAP 1925
>SPBC1A4.08c |cct3||chaperonin-containing T-complex gamma subunit
Cct3|Schizosaccharomyces pombe|chr 2|||Manual
Length = 528
Score = 26.2 bits (55), Expect = 6.1
Identities = 13/38 (34%), Positives = 18/38 (47%)
Frame = +1
Query: 205 EYGYTSKIHXVQCWERTAVEVKLVDTGFIRTCLLX*VD 318
E G +H WE AV+++ + T CLL VD
Sbjct: 480 ETGRVVDMHEYGVWEPEAVKLQSIKTAIESACLLLRVD 517
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 25.8 bits (54), Expect = 8.1
Identities = 18/67 (26%), Positives = 21/67 (31%), Gaps = 1/67 (1%)
Frame = +2
Query: 389 PXXXXRSXGXPFXSPXKXP-KXSIFXSPXVPPXRGPPXPXXNFIKKXSPPLFPPIGXXXP 565
P S PF P + F P VP P P + PP+ PP P
Sbjct: 474 PSNLVPSQNAPFIPGTSAPLPPTTFAPPGVPLPPIPGAPGMPNLNMSQPPMVPPGMALPP 533
Query: 566 XFNPPXP 586
P P
Sbjct: 534 GMPAPFP 540
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,378,618
Number of Sequences: 5004
Number of extensions: 35378
Number of successful extensions: 124
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 73
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 107
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 436477420
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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