Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_H22
(942 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 38 0.003
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 34 0.025
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 32 0.10
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.54
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.95
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 28 1.7
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 28 2.2
SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31 |Schizo... 28 2.2
SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase Ppk29|Schizos... 23 6.3
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 6.7
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 26 6.7
SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein Sap49|Schizo... 26 6.7
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 37.5 bits (83), Expect = 0.003
Identities = 24/82 (29%), Positives = 26/82 (31%), Gaps = 2/82 (2%)
Frame = +3
Query: 459 PXPXKNXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXP--XXXPPPPPTXXXXPXPPN 632
P +N PP G PPPPP G P PPPPP P+
Sbjct: 316 PPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPPQGRSAPPPPPPR----SAPS 371
Query: 633 RGGXXPXXKXXXXXLXPXXPPP 698
G P P PPP
Sbjct: 372 TGRQPPPLSSSRAVSNPPAPPP 393
Score = 30.7 bits (66), Expect = 0.31
Identities = 18/59 (30%), Positives = 20/59 (33%)
Frame = +3
Query: 522 PPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPPNRGGXXPXXKXXXXXLXPXXPPP 698
PP PP ++ PPPPP P NRG L P PPP
Sbjct: 290 PPLPPPSSRVSAAALAANKKRPPPPPP-----PSRRNRGKPPIGNGSSNSSLPPPPPPP 343
Score = 29.1 bits (62), Expect = 0.95
Identities = 12/30 (40%), Positives = 14/30 (46%)
Frame = -1
Query: 453 PPPPXAXXKXXXPPPXPAFXRAPXXPPXPP 364
PPPP + PP + RA PP PP
Sbjct: 363 PPPPRSAPSTGRQPPPLSSSRAVSNPPAPP 392
Score = 27.5 bits (58), Expect = 2.9
Identities = 16/54 (29%), Positives = 16/54 (29%)
Frame = +3
Query: 465 PXKNXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXP 626
P P PP G PP PP G P P PP P P
Sbjct: 428 PPSLPPSAPPSLPMGAPAAPPLPPS-APIAPPLPAGMPAAPPLPPAAPAPPPAP 480
Score = 27.1 bits (57), Expect = 3.8
Identities = 21/79 (26%), Positives = 24/79 (30%), Gaps = 2/79 (2%)
Frame = +3
Query: 474 NXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPPNRGGXXPX 653
N P PPP PP ++T P PP P PP+ P
Sbjct: 387 NPPAPPPAIPGRSAPALPPLGNASRT-----STPPVPTPPSLPPSAPPSLPPSAPPSLPM 441
Query: 654 XKXXXXXLXPXXP--PPXP 704
L P P PP P
Sbjct: 442 GAPAAPPLPPSAPIAPPLP 460
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 34.3 bits (75), Expect = 0.025
Identities = 15/41 (36%), Positives = 16/41 (39%)
Frame = +3
Query: 480 PPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPP 602
PPPPP G PPPPP + G P P P
Sbjct: 763 PPPPPPGVAGAGPPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
Score = 33.9 bits (74), Expect = 0.033
Identities = 18/41 (43%), Positives = 18/41 (43%)
Frame = +3
Query: 480 PPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPP 602
P PPP G PPPPP G G P PPPPP
Sbjct: 750 PVPPPAPIMGGPP-PPPPP------PGVAGAGPPPPPPPPP 783
Score = 33.1 bits (72), Expect = 0.058
Identities = 18/53 (33%), Positives = 19/53 (35%)
Frame = +3
Query: 471 KNXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPP 629
K+ PPPPP P P P GG P PPP P PP
Sbjct: 730 KSPPPPPPAVIV---PTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPP 779
Score = 29.9 bits (64), Expect = 0.54
Identities = 19/54 (35%), Positives = 19/54 (35%), Gaps = 7/54 (12%)
Frame = -1
Query: 462 KGAPPPPXAXX-------KXXXPPPXPAFXRAPXXPPXPPGXAXFXXXGXXXPP 322
K PPPP A PPP P P PP PPG A PP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMG-GPPPPPPPPGVAGAGPPPPPPPP 782
Score = 29.1 bits (62), Expect = 0.95
Identities = 13/33 (39%), Positives = 13/33 (39%)
Frame = -1
Query: 453 PPPPXAXXKXXXPPPXPAFXRAPXXPPXPPGXA 355
PPP PPP P A PP PP A
Sbjct: 752 PPPAPIMGGPPPPPPPPGVAGAGPPPPPPPPPA 784
Score = 28.3 bits (60), Expect = 1.7
Identities = 19/61 (31%), Positives = 19/61 (31%)
Frame = +3
Query: 522 PPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPPNRGGXXPXXKXXXXXLXPXXPPPX 701
PPPPP P PPP P P PP G P PPP
Sbjct: 732 PPPPPPAVIVPTPAPA--PIPVPPPAPIMGGPPPPPPPPGVA--------GAGPPPPPPP 781
Query: 702 P 704
P
Sbjct: 782 P 782
Score = 27.1 bits (57), Expect = 3.8
Identities = 17/59 (28%), Positives = 18/59 (30%)
Frame = -2
Query: 575 RPPXPPXXGFCXLRXXXGPXXXPXXXPXGGGGXVFXGXRXPPPPPPRXKXXXXXPXXPP 399
+ P PP P P P GG PPPPPP P PP
Sbjct: 730 KSPPPPPPAVIVPTPAPAPIPVPPPAPIMGGPP-------PPPPPPGVAGAGPPPPPPP 781
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 32.3 bits (70), Expect = 0.10
Identities = 15/32 (46%), Positives = 15/32 (46%)
Frame = +3
Query: 453 GXPXPXKNXPPPPPGXXXGXXXXPPPPPQXTK 548
G P P PPPPPG PPPPP K
Sbjct: 7 GNPPP----PPPPPGFEPPSQPPPPPPPGYVK 34
Score = 31.5 bits (68), Expect = 0.18
Identities = 12/24 (50%), Positives = 12/24 (50%)
Frame = +3
Query: 465 PXKNXPPPPPGXXXGXXXXPPPPP 536
P N PPPPP PPPPP
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28
Score = 29.1 bits (62), Expect = 0.95
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +3
Query: 465 PXKNXPPPPPGXXXGXXXXPPPPP 536
P PPPPP PPPPP
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPP 29
Score = 26.6 bits (56), Expect = 5.1
Identities = 10/23 (43%), Positives = 11/23 (47%)
Frame = -2
Query: 467 GXRXPPPPPPRXKXXXXXPXXPP 399
G PPPPPP + P PP
Sbjct: 7 GNPPPPPPPPGFEPPSQPPPPPP 29
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.9 bits (64), Expect = 0.54
Identities = 17/46 (36%), Positives = 18/46 (39%)
Frame = +2
Query: 323 GGXKXPXXXXXAXPGGXGGXXGARXKAGXGGGXXXFXXAXGGGGAP 460
GG + PGG GG G G GGG F GG G P
Sbjct: 231 GGFEGGPGGFGGGPGGFGGGLG-----GFGGGPGGFGGGPGGHGGP 271
Score = 28.7 bits (61), Expect = 1.3
Identities = 18/48 (37%), Positives = 18/48 (37%)
Frame = -3
Query: 601 GGGGGXXXGXPPXPPXXVFVXCGGGGGXXXXPXLXPGGGGGXFXXGXG 458
GG GG G PP P GG G GGG G F G G
Sbjct: 191 GGFGGGSGGPPPGPGGFGGFGGFGGEGHHHGGHGGFGGGPGGFEGGPG 238
Score = 26.2 bits (55), Expect = 6.7
Identities = 10/16 (62%), Positives = 10/16 (62%)
Frame = -3
Query: 493 GGGGGXFXXGXGXPPP 446
GGG G F G G PPP
Sbjct: 187 GGGFGGFGGGSGGPPP 202
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 0.95
Identities = 19/63 (30%), Positives = 21/63 (33%)
Frame = +3
Query: 438 RXGGGGXPXPXKNXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXX 617
+ G P P + PP P G P PPP G P P P PT
Sbjct: 1183 KPAAGVPPVPPPSEAPPVPKPSVG--VPPVPPPSTAPPVPTPSAGLP---PVPVPTAKAP 1237
Query: 618 PXP 626
P P
Sbjct: 1238 PVP 1240
Score = 26.6 bits (56), Expect = 5.1
Identities = 18/64 (28%), Positives = 19/64 (29%)
Frame = +3
Query: 459 PXPXKNXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPPNRG 638
P P + PP P P P P T G P PP P P P
Sbjct: 1025 PLPSADAPPIP----VPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPAPSSEIPSIPAPS 1080
Query: 639 GXXP 650
G P
Sbjct: 1081 GAPP 1084
Score = 25.8 bits (54), Expect = 8.8
Identities = 19/72 (26%), Positives = 21/72 (29%), Gaps = 5/72 (6%)
Frame = +3
Query: 450 GGXPXPXKNXPPPP-PGXXXGXXXXPPP----PPQXTKTXXGGXGGXPXXXPPPPPTXXX 614
G P P + PP P G P P PP + PP PP
Sbjct: 1157 GAPPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTA 1216
Query: 615 XPXPPNRGGXXP 650
P P G P
Sbjct: 1217 PPVPTPSAGLPP 1228
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 28.3 bits (60), Expect = 1.7
Identities = 17/57 (29%), Positives = 17/57 (29%)
Frame = +3
Query: 459 PXPXKNXPPPPPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPP 629
P P PPPP P P G PPPP T P PP
Sbjct: 234 PLPAPPPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQ----VNIPPPPATPSQPPRPP 286
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.9 bits (59), Expect = 2.2
Identities = 12/33 (36%), Positives = 14/33 (42%)
Frame = -1
Query: 453 PPPPXAXXKXXXPPPXPAFXRAPXXPPXPPGXA 355
PPPP + PP PA + PP P A
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSA 1740
Score = 27.5 bits (58), Expect = 2.9
Identities = 12/27 (44%), Positives = 13/27 (48%), Gaps = 2/27 (7%)
Frame = +3
Query: 459 PXPXKNXPPPP--PGXXXGXXXXPPPP 533
P P + PPPP P G PPPP
Sbjct: 1708 PPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 27.1 bits (57), Expect = 3.8
Identities = 11/31 (35%), Positives = 11/31 (35%)
Frame = -1
Query: 456 APPPPXAXXKXXXPPPXPAFXRAPXXPPXPP 364
AP PP PP P P P PP
Sbjct: 1704 APTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPBC8D2.20c |sec31||COPII-coated vesicle component Sec31
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1224
Score = 27.9 bits (59), Expect = 2.2
Identities = 14/40 (35%), Positives = 15/40 (37%)
Frame = +3
Query: 522 PPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPPNRGG 641
PPPPP + T P PPPP PP G
Sbjct: 908 PPPPPTASMT-----ASAPAIASPPPPKVGETYHPPTASG 942
>SPBC557.04 |ppk29||Ark1/Prk1 family protein kinase
Ppk29|Schizosaccharomyces pombe|chr 2|||Manual
Length = 872
Score = 22.6 bits (46), Expect(2) = 6.3
Identities = 8/18 (44%), Positives = 8/18 (44%)
Frame = -2
Query: 455 PPPPPPRXKXXXXXPXXP 402
PPPPPP P P
Sbjct: 356 PPPPPPMPAPIYNVPNVP 373
Score = 21.8 bits (44), Expect(2) = 6.3
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = -2
Query: 467 GXRXPPPPP 441
G R PPPPP
Sbjct: 353 GSRPPPPPP 361
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 6.7
Identities = 17/70 (24%), Positives = 19/70 (27%)
Frame = +3
Query: 489 PPGXXXGXXXXPPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXPPNRGGXXPXXKXXX 668
PP PPP P P PPP PP+ P
Sbjct: 139 PPTSAPPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPS----APSLPSAV 194
Query: 669 XXLXPXXPPP 698
+ P PPP
Sbjct: 195 PPMPPKVPPP 204
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 26.2 bits (55), Expect = 6.7
Identities = 11/27 (40%), Positives = 11/27 (40%)
Frame = -1
Query: 453 PPPPXAXXKXXXPPPXPAFXRAPXXPP 373
PPPP A K P P P PP
Sbjct: 1884 PPPPMALPKAGPPSAAPTSALPPAGPP 1910
>SPAC31G5.01 |sap49|SPAPB1A11.05|RNA-binding protein
Sap49|Schizosaccharomyces pombe|chr 1|||Manual
Length = 335
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/35 (34%), Positives = 12/35 (34%)
Frame = +3
Query: 522 PPPPPQXTKTXXGGXGGXPXXXPPPPPTXXXXPXP 626
P P G GG P PPPP P P
Sbjct: 269 PGMPAMPMMNVPMGPGGAPLVPPPPPGMVMASPSP 303
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,656,281
Number of Sequences: 5004
Number of extensions: 29695
Number of successful extensions: 296
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 47
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 196
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 479324640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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