Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_D21
(877 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces pomb... 54 2e-08
SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 53 6e-08
SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces pomb... 50 3e-07
SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 48 1e-06
SPAC3A12.03c |mug145||ubiquitin-protein ligase E3 |Schizosacchar... 46 7e-06
SPCC126.07c |||human CTD-binding SR-like protein rA9 homolog|Sch... 44 2e-05
SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 42 9e-05
SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 41 2e-04
SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3 |Schizos... 41 2e-04
SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|ch... 40 4e-04
SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 38 0.002
SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 37 0.004
SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8 |Schizosacch... 35 0.017
SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein 1|Schizosaccharom... 34 0.023
SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces ... 33 0.040
SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces pom... 32 0.093
SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyce... 31 0.16
SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2 |Schizo... 31 0.28
SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3 |Schi... 30 0.38
SPAC343.03 |apc11||anaphase-promoting complex subunit Apc11|Schi... 30 0.38
SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces... 30 0.38
SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces p... 30 0.50
SPAC17G8.10c |dma1||mitotic spindle checkpoint protein Dma1|Schi... 29 0.66
SPCC18.03 |||shuttle craft like transcriptional regulator|Schizo... 29 0.87
SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein P... 27 2.7
SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pomb... 27 3.5
SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2 |Schizosac... 27 3.5
SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyc... 26 6.1
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 8.1
>SPAC57A7.09 |||human RNF family homolog|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 372
Score = 54.4 bits (125), Expect = 2e-08
Identities = 21/47 (44%), Positives = 29/47 (61%), Gaps = 1/47 (2%)
Frame = +2
Query: 350 CPICLKNLEAGEKVKKMPCNHIFHPXCILTWL-DXTNSCPFCXXEMP 487
C ICL++ G+KV +PC H FH CI W+ D ++CP C E+P
Sbjct: 321 CVICLESFTKGDKVVALPCKHEFHRPCIAKWIVDYRHACPTCNTEVP 367
>SPBP4H10.07 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 583
Score = 52.8 bits (121), Expect = 6e-08
Identities = 25/67 (37%), Positives = 37/67 (55%), Gaps = 3/67 (4%)
Frame = +2
Query: 281 PPPASKEAVQKLQDVV-IENQKKGCPICLKNLEAGEKVKKMP-CNHIFHPXCILTWLDXT 454
PP AS++ ++K V + C +CL N E ++ +++ CNH FH CI WL +
Sbjct: 501 PPVASQKDLEKAGGVFPFSGTDERCLVCLSNFELNDECRRLKQCNHFFHRECIDQWLTSS 560
Query: 455 -NSCPFC 472
NSCP C
Sbjct: 561 QNSCPLC 567
>SPBC2A9.04c |||sir antagonist ortholog |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 741
Score = 50.4 bits (115), Expect = 3e-07
Identities = 22/48 (45%), Positives = 28/48 (58%), Gaps = 2/48 (4%)
Frame = +2
Query: 350 CPICLKNL-EAGEK-VKKMPCNHIFHPXCILTWLDXTNSCPFCXXEMP 487
CPIC ++ E EK KMPC HIF C+ WL+ +CP C E+P
Sbjct: 107 CPICYDDMNENDEKQATKMPCGHIFGKNCLQKWLENHCTCPLCRKEVP 154
>SPAP32A8.03c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 513
Score = 48.4 bits (110), Expect = 1e-06
Identities = 20/74 (27%), Positives = 38/74 (51%), Gaps = 4/74 (5%)
Frame = +2
Query: 263 AEWPSLPPPASKEAVQKLQ----DVVIENQKKGCPICLKNLEAGEKVKKMPCNHIFHPXC 430
A+ + P PA ++ + K++ + +++ C IC++ + + V ++PC H FH C
Sbjct: 363 AQGHNAPAPAPEDVIAKMKVQKPPKELIDEEGECTICMEMFKINDDVIQLPCKHYFHENC 422
Query: 431 ILTWLDXTNSCPFC 472
I WL +C C
Sbjct: 423 IKPWLRVNGTCAIC 436
>SPAC3A12.03c |mug145||ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 309
Score = 46.0 bits (104), Expect = 7e-06
Identities = 17/50 (34%), Positives = 29/50 (58%), Gaps = 1/50 (2%)
Frame = +2
Query: 326 VIENQKKGCPICLKNLEAGEKVKKMPCNHIFHPXCILTWLDXTN-SCPFC 472
++E+++ C IC + + ++ +PC H+FH CI TW+ SCP C
Sbjct: 197 LLEDEEDFCIICYADYAFDDILRVLPCEHVFHTQCIDTWMTTMKASCPLC 246
>SPCC126.07c |||human CTD-binding SR-like protein rA9
homolog|Schizosaccharomyces pombe|chr 3|||Manual
Length = 571
Score = 44.4 bits (100), Expect = 2e-05
Identities = 15/43 (34%), Positives = 23/43 (53%)
Frame = +2
Query: 353 PICLKNLEAGEKVKKMPCNHIFHPXCILTWLDXTNSCPFCXXE 481
P + + G ++ K+PC H FH C+ +W N+CP C E
Sbjct: 39 PASISSTLDGLRIAKIPCGHYFHNHCLESWCRVANTCPLCRTE 81
>SPCC4G3.12c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 821
Score = 42.3 bits (95), Expect = 9e-05
Identities = 19/43 (44%), Positives = 23/43 (53%), Gaps = 2/43 (4%)
Frame = +2
Query: 350 CPICLKNLEAGEKVKKMP-CNHIFHPXCILTWL-DXTNSCPFC 472
C ICL+ G+ +K+ C H FH CI WL NSCP C
Sbjct: 766 CLICLETYTNGDICRKLQACKHFFHQACIDQWLTTGNNSCPLC 808
>SPBC947.10 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 676
Score = 41.1 bits (92), Expect = 2e-04
Identities = 11/29 (37%), Positives = 19/29 (65%)
Frame = +2
Query: 401 PCNHIFHPXCILTWLDXTNSCPFCXXEMP 487
PC+H++H C+L W++ + CP C +P
Sbjct: 646 PCHHLYHRQCLLQWMETRSICPVCRCHLP 674
>SPBC15C4.06c ||SPBC21H7.01c|ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 556
Score = 41.1 bits (92), Expect = 2e-04
Identities = 18/46 (39%), Positives = 28/46 (60%), Gaps = 2/46 (4%)
Frame = +2
Query: 341 KKGCPICL-KNLEAGEKVKKMPCNHIFHPXCILTW-LDXTNSCPFC 472
++ C ICL + E +++PC+HIFHP CI + L ++ CP C
Sbjct: 495 QRECTICLCEYSEESPLYRELPCHHIFHPACIDPYLLKNSDLCPLC 540
>SPBC17D11.02c |||synoviolin homolog|Schizosaccharomyces pombe|chr
2|||Manual
Length = 677
Score = 40.3 bits (90), Expect = 4e-04
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = +2
Query: 392 KKMPCNHIFHPXCILTWLDXTNSCPFC 472
K++PC HI H C+ WL+ +CP C
Sbjct: 324 KRLPCGHILHFHCLRNWLERQQTCPIC 350
>SPCC548.05c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 3|||Manual
Length = 468
Score = 37.9 bits (84), Expect = 0.002
Identities = 17/47 (36%), Positives = 24/47 (51%)
Frame = +2
Query: 350 CPICLKNLEAGEKVKKMPCNHIFHPXCILTWLDXTNSCPFCXXEMPT 490
CPIC EA ++ C H + C+L WL + SCP C ++ T
Sbjct: 85 CPICT---EALQRPFTTHCGHTYCYECLLNWLKESKSCPTCRQKLYT 128
>SPBC16G5.03 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 268
Score = 36.7 bits (81), Expect = 0.004
Identities = 16/45 (35%), Positives = 25/45 (55%), Gaps = 1/45 (2%)
Frame = +2
Query: 341 KKGCPICLKNLEAGEKVKKMPCNHI-FHPXCILTWLDXTNSCPFC 472
++ C ICL+ + G + + PC H F CI W+D + +CP C
Sbjct: 18 RRDCVICLQ--KDGLRAQLSPCGHDQFDYSCICRWMDQSLTCPIC 60
>SPBC3D6.11c |slx8||ubiquitin-protein ligase E3 Slx8
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 269
Score = 34.7 bits (76), Expect = 0.017
Identities = 23/62 (37%), Positives = 30/62 (48%), Gaps = 3/62 (4%)
Frame = +2
Query: 296 KEAVQKLQDVVIENQKKGCPICLKNLEAGEKVKKMPCNHIFHPXCILTWLD---XTNSCP 466
KE K Q VV +Q+ C+ L++ E + PC HIF CIL+ L T CP
Sbjct: 186 KEEPSKKQ-VVPSSQRLADYKCVICLDSPENLSCTPCGHIFCNFCILSALGTTAATQKCP 244
Query: 467 FC 472
C
Sbjct: 245 VC 246
>SPAC23H4.18c |rbx1|pip1, pip1|RING-box protein
1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 107
Score = 34.3 bits (75), Expect = 0.023
Identities = 12/21 (57%), Positives = 13/21 (61%)
Frame = +2
Query: 404 CNHIFHPXCILTWLDXTNSCP 466
CNH FH CI WL+ N CP
Sbjct: 74 CNHAFHFHCISRWLNTRNVCP 94
>SPAC16E8.13 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 547
Score = 33.5 bits (73), Expect = 0.040
Identities = 21/91 (23%), Positives = 41/91 (45%), Gaps = 2/91 (2%)
Frame = +2
Query: 206 HLLHFARFLIDSGLDRTGAAEWPS-LPPPASKEAVQKLQDVVIENQKKGCPICLKNLEAG 382
H+LH + I ++ + ++ L P+SK ++ + C +CL+ +++
Sbjct: 162 HVLHIDKVNIKYPMESSDSSSTEQQLVGPSSKPFASTTPALI---ELPTCVVCLERMDSS 218
Query: 383 -EKVKKMPCNHIFHPXCILTWLDXTNSCPFC 472
+ + C H FH C+ W +SCP C
Sbjct: 219 ITGLITIVCQHTFHCPCLQKW--GNSSCPVC 247
>SPAC144.05 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1375
Score = 32.3 bits (70), Expect = 0.093
Identities = 9/27 (33%), Positives = 16/27 (59%)
Frame = +2
Query: 404 CNHIFHPXCILTWLDXTNSCPFCXXEM 484
C H++ C+ WL ++SCP C ++
Sbjct: 1107 CGHLYCSFCLEAWLKHSSSCPMCKTKL 1133
>SPBC21D10.09c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1610
Score = 31.5 bits (68), Expect = 0.16
Identities = 15/46 (32%), Positives = 18/46 (39%), Gaps = 5/46 (10%)
Frame = +2
Query: 350 CPICLKNLEAGEKVKKM---PCNHIFHPXCILTWLDXTNS--CPFC 472
C IC L + C H FH C+ W +NS CP C
Sbjct: 1558 CAICYSVLSVERTLPNKRCGTCRHKFHASCLYKWFKSSNSSRCPLC 1603
>SPBP19A11.06 |lid2|SPBP4H10.01|Lid2 complex subunit Lid2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 1513
Score = 30.7 bits (66), Expect = 0.28
Identities = 17/54 (31%), Positives = 27/54 (50%), Gaps = 3/54 (5%)
Frame = +2
Query: 281 PP--PASKEAVQKLQDVVIENQKKGCPICLKNL-EAGEKVKKMPCNHIFHPXCI 433
PP PAS+E + + +KKGC C L E+G ++ C+ +H C+
Sbjct: 1071 PPMEPASRETSPDSEGRLTIRKKKGCIFCFCRLPESGVMIECEICHEWYHAKCL 1124
>SPBC17A3.10 |pas4||peroxisomal ubiquitin-protein ligase E3
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 306
Score = 30.3 bits (65), Expect = 0.38
Identities = 14/47 (29%), Positives = 21/47 (44%)
Frame = +2
Query: 332 ENQKKGCPICLKNLEAGEKVKKMPCNHIFHPXCILTWLDXTNSCPFC 472
E +K C +C++ + + C HIF CI W + CP C
Sbjct: 251 EGNRK-CSLCMEFIHCPAATE---CGHIFCWSCINGWTSKKSECPLC 293
>SPAC343.03 |apc11||anaphase-promoting complex subunit
Apc11|Schizosaccharomyces pombe|chr 1|||Manual
Length = 94
Score = 30.3 bits (65), Expect = 0.38
Identities = 12/29 (41%), Positives = 14/29 (48%)
Frame = +2
Query: 404 CNHIFHPXCILTWLDXTNSCPFCXXEMPT 490
C HIFH CI WL + S C + T
Sbjct: 52 CKHIFHAHCIQNWLATSGSQGQCPMDRQT 80
>SPBC14F5.10c |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 486
Score = 30.3 bits (65), Expect = 0.38
Identities = 18/61 (29%), Positives = 29/61 (47%), Gaps = 2/61 (3%)
Frame = +2
Query: 314 LQDVVIENQKKG--CPICLKNLEAGEKVKKMPCNHIFHPXCILTWLDXTNSCPFCXXEMP 487
++D++ EN + C IC L + V PC H F C++ L + CP C +P
Sbjct: 155 MEDLLQENVSRELECQICFGMLY--DPVVS-PCGHTFCGPCLMQALTQSPQCPTCRFGLP 211
Query: 488 T 490
+
Sbjct: 212 S 212
>SPAC2F3.16 |||ubiquitin-protein ligase E3 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 425
Score = 29.9 bits (64), Expect = 0.50
Identities = 13/42 (30%), Positives = 21/42 (50%), Gaps = 1/42 (2%)
Frame = +2
Query: 350 CPICLKNL-EAGEKVKKMPCNHIFHPXCILTWLDXTNSCPFC 472
CPIC + + + E+V + C+H H C ++ CP C
Sbjct: 271 CPICGEYMFNSRERVIFLSCSHPLHQRCHEEYIRTNYRCPTC 312
>SPAC17G8.10c |dma1||mitotic spindle checkpoint protein
Dma1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 267
Score = 29.5 bits (63), Expect = 0.66
Identities = 19/75 (25%), Positives = 35/75 (46%), Gaps = 7/75 (9%)
Frame = +2
Query: 269 WPSLPPPASKEAVQKLQDVVI----ENQKKGCPICLKNLEAGEKVKKMPCNHIFHPXCIL 436
W P + +++Q++V+ E+ C ICL + + + PC+H +H CI
Sbjct: 161 WKIKLSPYNLNEFKRMQELVLCGSSESGPPECCICLMPVLPCQALFVAPCSHSYHYKCIR 220
Query: 437 TWLDXTN---SCPFC 472
L+ ++ SC C
Sbjct: 221 PTLNESHPYFSCFIC 235
>SPCC18.03 |||shuttle craft like transcriptional
regulator|Schizosaccharomyces pombe|chr 3|||Manual
Length = 1077
Score = 29.1 bits (62), Expect = 0.87
Identities = 15/59 (25%), Positives = 25/59 (42%), Gaps = 9/59 (15%)
Frame = +2
Query: 323 VVIENQKKGCPICLKNLEAGEKVKKM-PCNHIFHPXCILTW----LDXTNS----CPFC 472
V ++N+ C +C + + C H+FH CI W ++ N CP+C
Sbjct: 188 VELKNRLYECSVCTDTINPSTSIWSCGTCYHVFHLSCIRKWCKNSIEQRNEDAWRCPYC 246
>SPAC27E2.07 |pvg2|mug53|galactose residue biosynthesis protein
Pvg2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 389
Score = 27.5 bits (58), Expect = 2.7
Identities = 12/24 (50%), Positives = 16/24 (66%)
Frame = -3
Query: 314 IFVLPLSMQAEVSLATPLHQFDLS 243
IF+ P S+Q + +ATP H F LS
Sbjct: 242 IFMAPKSLQYDFMIATPKHPFVLS 265
>SPAC3H5.09c |||conserved fungal protein|Schizosaccharomyces pombe|chr
1|||Manual
Length = 2685
Score = 27.1 bits (57), Expect = 3.5
Identities = 12/27 (44%), Positives = 17/27 (62%)
Frame = -3
Query: 332 LLQHLVIFVLPLSMQAEVSLATPLHQF 252
++QH I + PLS+Q E LAT L +
Sbjct: 2342 VIQHFEINLFPLSLQIEQELATKLFAY 2368
>SPAC16A10.06c |nse2||Smc5-6 complex non-SMC subunit 2
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 250
Score = 27.1 bits (57), Expect = 3.5
Identities = 13/44 (29%), Positives = 20/44 (45%)
Frame = +2
Query: 350 CPICLKNLEAGEKVKKMPCNHIFHPXCILTWLDXTNSCPFCXXE 481
CP+ L+ + + CNH + IL+ L+ T CP E
Sbjct: 179 CPLTLQPIV--HPILSTACNHFYEKDAILSLLNPTCVCPVVGCE 220
>SPCC962.06c |bpb1|sf1|zinc finger protein Bpb1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 587
Score = 26.2 bits (55), Expect = 6.1
Identities = 16/46 (34%), Positives = 24/46 (52%), Gaps = 5/46 (10%)
Frame = -3
Query: 308 VLPLSMQAEVSLAT-----PLHQFDLSQNL*EILQNVRDDLVVPHH 186
+LP M+ E ++ + L + L+ L EI Q +R VVPHH
Sbjct: 82 MLPSQMELETAVKSCMTMEQLELYSLNVRLEEITQKLRTGDVVPHH 127
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 25.8 bits (54), Expect = 8.1
Identities = 11/22 (50%), Positives = 12/22 (54%), Gaps = 2/22 (9%)
Frame = +3
Query: 771 LPPXPXXPPFP--PXFXXPPPL 830
+PP P PP P P PPPL
Sbjct: 1714 VPPPPSAPPMPAGPPSAPPPPL 1735
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,602,912
Number of Sequences: 5004
Number of extensions: 46520
Number of successful extensions: 181
Number of sequences better than 10.0: 29
Number of HSP's better than 10.0 without gapping: 156
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 176
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 438479610
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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