Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_D18
(949 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 31 0.24
SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 30 0.41
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.55
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.3
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 28 1.7
SPAC22F8.11 |plc1||phosphoinositide phospholipase C Plc1|Schizos... 28 2.2
SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78 |Schizosacch... 28 2.2
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.9
SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2 |Schizosacch... 27 2.9
SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces... 27 5.1
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 26 6.7
SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation... 26 6.7
SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces p... 26 8.9
SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyce... 26 8.9
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 31.1 bits (67), Expect = 0.24
Identities = 26/95 (27%), Positives = 47/95 (49%), Gaps = 6/95 (6%)
Frame = +3
Query: 240 TLEQQFNSLTKSKDAQD---FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQ 410
T+E ++SL KSK + F + ++ + +L N + + L + +A KA+++LEQ
Sbjct: 8 TIELDYDSL-KSKISNFNSIFDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQ 65
Query: 411 SRQNIERTAEELRKAHPD---VEKNATXLREKLXA 506
+ + E L K H + E+ +EKL A
Sbjct: 66 TEARKQNFTELLEKEHEEQAITEQEIFSFQEKLDA 100
>SPAPB1A10.08 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 412
Score = 30.3 bits (65), Expect = 0.41
Identities = 27/113 (23%), Positives = 47/113 (41%)
Frame = +3
Query: 93 PHSVSRQYIMAAKFVVLFACIALAQGAMVRRDAPDFFKDIEHHTKEFHKTLEQQFNSLTK 272
P S S + A + L + V R F + +EH+ K+LE+Q + L +
Sbjct: 171 PSSSSCNLVNANSLDIYLNINNLKKSKSVPRLRGQFMEPVEHN-HPLSKSLEEQSSFLEQ 229
Query: 273 SKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIER 431
SKDA A S+ + S + +L N K++ +L+ ++ R
Sbjct: 230 SKDASSNLTACNRSGSSLSSNFYSSRLSKKTSLASLN-KSRASLQHKIMSLSR 281
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.55
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +3
Query: 306 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 443
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.3
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +3
Query: 285 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 440
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 28.3 bits (60), Expect = 1.7
Identities = 24/88 (27%), Positives = 45/88 (51%)
Frame = +3
Query: 210 IEHHTKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANGK 389
IE K F K ++ NS K+ +A F +G++ ++ + A ++L L +
Sbjct: 73 IESSMKSF-KPVKIDLNSQLKAINA--FEAKASEGAKKNVELVKAELQNLSATLKN---- 125
Query: 390 AKEALEQSRQNIERTAEELRKAHPDVEK 473
+EQ+R E T E++++A P++EK
Sbjct: 126 ----IEQARPTEEITIEDMKQAVPEIEK 149
>SPAC22F8.11 |plc1||phosphoinositide phospholipase C
Plc1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 899
Score = 27.9 bits (59), Expect = 2.2
Identities = 18/70 (25%), Positives = 32/70 (45%), Gaps = 3/70 (4%)
Frame = +3
Query: 180 RRDAPDFFKDIEHHTKEFHKTLEQQFNSLT---KSKDAQDFSKAWKDGSESVLQQLNAFA 350
++ DFFK + ++ H TL ++ NSL+ +K + G + +LN
Sbjct: 56 KKSEQDFFKMLSSRDRDAHSTLRKRSNSLSSFLSTKSTSASENKFHGGLNWLSLKLNLLL 115
Query: 351 KSLQGALGDA 380
+ LQG + A
Sbjct: 116 R-LQGRMNSA 124
>SPBC4F6.17c |||mitochondrial matrix chaperone Hsp78
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 803
Score = 27.9 bits (59), Expect = 2.2
Identities = 24/74 (32%), Positives = 33/74 (44%), Gaps = 6/74 (8%)
Frame = +3
Query: 240 TLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGA------LGDANGKAKEA 401
T++ + SL K D + ++ ES L L L A L D+ KAK
Sbjct: 354 TIQIELESLRKETDTTSVER--REKLESKLTDLKEEQDKLSAAWEEERKLLDSIKKAKTE 411
Query: 402 LEQSRQNIERTAEE 443
LEQ+R +ERT E
Sbjct: 412 LEQARIELERTQRE 425
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.9
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -2
Query: 417 ASTVPKPPWPCRSRLRALPG 358
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPBC947.02 |apl2||AP-1 adaptor complex subunit Apl2
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 683
Score = 27.5 bits (58), Expect = 2.9
Identities = 14/39 (35%), Positives = 19/39 (48%)
Frame = +3
Query: 18 LTIGNSLRFKLLDTLLCVAVRFASPPHSVSRQYIMAAKF 134
++ N+L LLD LLC AS H + +I KF
Sbjct: 552 VSYNNNLPEALLDALLCEITTLASVYHKLPESFIGQGKF 590
>SPBC1A4.03c |top2|ptr11|DNA topoisomerase II|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 1485
Score = 26.6 bits (56), Expect = 5.1
Identities = 12/42 (28%), Positives = 19/42 (45%), Gaps = 2/42 (4%)
Frame = +3
Query: 186 DAPDFFKDIEHHTKEFHKTLEQ--QFNSLTKSKDAQDFSKAW 305
D +F D++ H K FH E+ + + +K D K W
Sbjct: 665 DMKSYFSDLDRHMKYFHAMQEKDAELIEMAFAKKKADVRKEW 706
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.2 bits (55), Expect = 6.7
Identities = 17/57 (29%), Positives = 25/57 (43%), Gaps = 5/57 (8%)
Frame = +3
Query: 354 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATXLREKLXAA 509
S++ L + N + KE +E + RT +E EKN LRE+L A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPAC32A11.04c |tif212|tif22, SPAC6B12.17c|translation initiation
factor eIF2 beta subunit|Schizosaccharomyces pombe|chr
1|||Manual
Length = 321
Score = 26.2 bits (55), Expect = 6.7
Identities = 16/54 (29%), Positives = 25/54 (46%), Gaps = 1/54 (1%)
Frame = +3
Query: 222 TKEFHKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQ-GALGDA 380
+K+ + E+Q +T DFS K + L+AF K L+ + GDA
Sbjct: 89 SKKSSASAEEQTEDITTESGELDFSSMKKKKKKKKSADLSAFEKELEASSTGDA 142
>SPAC18B11.02c |||pseudouridylate synthase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 394
Score = 25.8 bits (54), Expect = 8.9
Identities = 16/64 (25%), Positives = 34/64 (53%), Gaps = 1/64 (1%)
Frame = +3
Query: 150 CIALAQGAMVRRDAPDFFKDIEHHTKEFH-KTLEQQFNSLTKSKDAQDFSKAWKDGSESV 326
C + +G +RR P +++ I ++ KTL + FN+ + +++ + KA ++G V
Sbjct: 13 CEYVFKGDGLRRVPPYYYEYITFAKLRWYGKTLLEVFNTEFRDRESGYYEKAIRNGQVKV 72
Query: 327 LQQL 338
Q+
Sbjct: 73 NNQI 76
>SPAC1F3.06c |spo15||sporulation protein Spo15|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1957
Score = 25.8 bits (54), Expect = 8.9
Identities = 18/77 (23%), Positives = 35/77 (45%), Gaps = 3/77 (3%)
Frame = +3
Query: 234 HKTLEQQFNSLTKSKDAQDFSKAWKDGSESVLQQLNAFAKSLQGALGDANG---KAKEAL 404
H+T+ +Q + +A + ES L N ++ L ++N K +E +
Sbjct: 625 HQTITKQLKDTSSKLQQLQLERANFEQKESTLSDENNDLRTKLLKLEESNKSLIKKQEDV 684
Query: 405 EQSRQNIERTAEELRKA 455
+ +NI+ E+LRK+
Sbjct: 685 DSLEKNIQTLKEDLRKS 701
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,551,870
Number of Sequences: 5004
Number of extensions: 39036
Number of successful extensions: 144
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 136
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 144
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 483319012
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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