BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_D16
(943 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 30 0.54
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 29 0.72
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.95
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 28 2.2
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 28 2.2
SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit 1|Schizosacchar... 27 2.9
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 27 3.8
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 26 6.7
SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr 1|... 26 6.7
SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein ... 26 8.8
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 26 8.8
SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr 1... 23 9.6
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 29.9 bits (64), Expect = 0.54
Identities = 18/64 (28%), Positives = 19/64 (29%)
Frame = +1
Query: 652 PPXGGXXPXSPXNXRGXPPXKXPXPPPXPPPXPXXXXXXXXXXXVFXXXXXXXXXXPPKX 831
PP P SP + P P P PPP PPK
Sbjct: 144 PPRPSIPPPSPASAPPIPSKAPPIPSSLPPPAQPAAPVKSPPSA--PSLPSAVPPMPPKV 201
Query: 832 PPPP 843
PPPP
Sbjct: 202 PPPP 205
Score = 27.1 bits (57), Expect = 3.8
Identities = 13/33 (39%), Positives = 14/33 (42%), Gaps = 1/33 (3%)
Frame = +1
Query: 649 PPPXGGXXPX-SPXNXRGXPPXKXPXPPPXPPP 744
PPP P SP + P P PP PPP
Sbjct: 172 PPPAQPAAPVKSPPSAPSLPSAVPPMPPKVPPP 204
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 29.5 bits (63), Expect = 0.72
Identities = 12/21 (57%), Positives = 12/21 (57%)
Frame = +3
Query: 858 PPPPXXSNXTXXSPXKXPPRP 920
PP P SN T SP PPRP
Sbjct: 254 PPIPPPSNGTVSSPPNSPPRP 274
Score = 26.2 bits (55), Expect = 6.7
Identities = 13/31 (41%), Positives = 13/31 (41%)
Frame = +1
Query: 652 PPXGGXXPXSPXNXRGXPPXKXPXPPPXPPP 744
PP P S R P P PPP PPP
Sbjct: 230 PPIPPSIPSSRPPER-VPSLSAPAPPPIPPP 259
Score = 26.2 bits (55), Expect = 6.7
Identities = 21/85 (24%), Positives = 21/85 (24%), Gaps = 1/85 (1%)
Frame = +2
Query: 617 PAXXXXXXPXXPPPXGGXXXFPPXTXGXXPPKKXXXPPRXPPRXPXPXXRAPXGGGFSXX 796
PA P PPP PP PP R P
Sbjct: 282 PAINSTSKPPLPPPSSRVSAAALAANKKRPPPPP--PPSRRNRGKPPIGNGSSNSSLPPP 339
Query: 797 XXXXXXNXXQKXP-PPXXXGAPPPP 868
N P PP APPPP
Sbjct: 340 PPPPRSNAAGSIPLPPQGRSAPPPP 364
Score = 26.2 bits (55), Expect = 6.7
Identities = 19/76 (25%), Positives = 19/76 (25%)
Frame = +2
Query: 641 PXXPPPXGGXXXFPPXTXGXXPPKKXXXPPRXPPRXPXPXXRAPXGGGFSXXXXXXXXNX 820
P P P PP PP P PP P P G
Sbjct: 415 PPVPTPPSLPPSAPPSLPPSAPPSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLP---P 471
Query: 821 XQKXPPPXXXGAPPPP 868
PPP AP P
Sbjct: 472 AAPAPPPAPAPAPAAP 487
Score = 26.2 bits (55), Expect = 6.7
Identities = 11/33 (33%), Positives = 11/33 (33%)
Frame = +1
Query: 652 PPXGGXXPXSPXNXRGXPPXKXPXPPPXPPPXP 750
PP P P PP P P P P P
Sbjct: 450 PPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAP 482
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.1 bits (62), Expect = 0.95
Identities = 14/47 (29%), Positives = 15/47 (31%)
Frame = +2
Query: 641 PXXPPPXGGXXXFPPXTXGXXPPKKXXXPPRXPPRXPXPXXRAPXGG 781
P P P G PP + PK P PP P P G
Sbjct: 1179 PPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTPSAG 1225
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 27.9 bits (59), Expect = 2.2
Identities = 16/48 (33%), Positives = 16/48 (33%)
Frame = -2
Query: 783 PPPXGALXXGXGXRGGXRGGXXXFXGGXXPXVXGGXXXXPPXGGGXXG 640
P G G RGG GG F GG GG GG G
Sbjct: 5 PGSRGGRGGSRGGRGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGG 52
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 27.9 bits (59), Expect = 2.2
Identities = 18/50 (36%), Positives = 18/50 (36%), Gaps = 2/50 (4%)
Frame = -2
Query: 756 GXGXRGGXRGGXXXFXGGXXPXVXGGXXXXP--PXGGGXXGXXXXXXAGG 613
G G RGG RGG GG GG GGG G GG
Sbjct: 136 GRGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGGSRGGFRGG 185
>SPCC550.05 |nse1||Smc5-6 complex non-SMC subunit
1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 232
Score = 27.5 bits (58), Expect = 2.9
Identities = 9/23 (39%), Positives = 14/23 (60%)
Frame = +3
Query: 84 YCTNVSAC*LFSNVNSVNCDLVW 152
YC +V C ++VN +NC+ W
Sbjct: 201 YCLHVYCCKHLAHVNCINCNTPW 223
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 27.1 bits (57), Expect = 3.8
Identities = 11/31 (35%), Positives = 12/31 (38%)
Frame = +1
Query: 652 PPXGGXXPXSPXNXRGXPPXKXPXPPPXPPP 744
PP P + P P PPP PPP
Sbjct: 167 PPPSFQPPSAAAPATSLPSDYNPPPPPPPPP 197
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 26.2 bits (55), Expect = 6.7
Identities = 13/36 (36%), Positives = 13/36 (36%), Gaps = 2/36 (5%)
Frame = +1
Query: 649 PPPXGGXXPXSPXNXRGXPPXKXPXP--PPXPPPXP 750
PP P P PP P P PP PP P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
Score = 26.2 bits (55), Expect = 6.7
Identities = 12/31 (38%), Positives = 13/31 (41%)
Frame = +3
Query: 834 PPPXXRXRPPPPXXSNXTXXSPXKXPPRPXP 926
PPP PPPP + P PP P P
Sbjct: 1707 PPPPPMSVPPPPS-APPMPAGPPSAPPPPLP 1736
>SPAC20G4.02c |fus1||formin Fus1|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1372
Score = 26.2 bits (55), Expect = 6.7
Identities = 9/15 (60%), Positives = 9/15 (60%)
Frame = +1
Query: 706 PXKXPXPPPXPPPXP 750
P K P P P PPP P
Sbjct: 802 PFKAPPPAPLPPPAP 816
>SPBC119.05c |||Wiskott-Aldrich syndrome homolog binding protein
Lsb1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 296
Score = 25.8 bits (54), Expect = 8.8
Identities = 11/40 (27%), Positives = 15/40 (37%)
Frame = +2
Query: 650 PPPXGGXXXFPPXTXGXXPPKKXXXPPRXPPRXPXPXXRA 769
PPP +PP PP + P + P P +A
Sbjct: 206 PPPPPPQQNYPPAASSSAPPMQYQQTAYPPQQAPYPPVQA 245
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 25.8 bits (54), Expect = 8.8
Identities = 13/35 (37%), Positives = 14/35 (40%), Gaps = 1/35 (2%)
Frame = +1
Query: 649 PPPXGGXXPX-SPXNXRGXPPXKXPXPPPXPPPXP 750
PPP P +P PP PP PPP P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPP 768
Score = 25.8 bits (54), Expect = 8.8
Identities = 15/37 (40%), Positives = 15/37 (40%), Gaps = 3/37 (8%)
Frame = +1
Query: 649 PPPX---GGXXPXSPXNXRGXPPXKXPXPPPXPPPXP 750
PPP GG P P P PPP PPP P
Sbjct: 752 PPPAPIMGGPPPPPPP-----PGVAGAGPPPPPPPPP 783
>SPAC1F5.04c |cdc12||formin Cdc12|Schizosaccharomyces pombe|chr
1|||Manual
Length = 1841
Score = 23.0 bits (47), Expect(2) = 9.6
Identities = 7/9 (77%), Positives = 7/9 (77%)
Frame = +1
Query: 724 PPPXPPPXP 750
PPP PPP P
Sbjct: 945 PPPPPPPPP 953
Score = 20.6 bits (41), Expect(2) = 9.6
Identities = 7/13 (53%), Positives = 7/13 (53%)
Frame = +1
Query: 820 PPKXPPPPXXGXA 858
PP PPPP A
Sbjct: 946 PPPPPPPPLVSAA 958
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,824,812
Number of Sequences: 5004
Number of extensions: 26337
Number of successful extensions: 193
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 57
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 136
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 479324640
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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