Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP11_F_A24
(901 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 261 1e-70
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 142 8e-35
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 125 7e-30
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 101 1e-22
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 84 2e-17
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 79 6e-16
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 67 4e-12
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 34 0.032
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 33 0.055
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 29 0.68
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 29 0.90
SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr... 29 1.2
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 29 1.2
SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 27 3.6
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 261 bits (639), Expect = 1e-70
Identities = 114/130 (87%), Positives = 124/130 (95%)
Frame = +1
Query: 118 DDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAXXK 297
++++AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G+MVGMGQKDSYVGDEA K
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 298 RGILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTX 477
RGILTLKYPIEHGI+ NWDDMEKIWHHTFYNELRVAPEEHP LLTEAPLNPK+NREKMT
Sbjct: 62 RGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPLNPKSNREKMTQ 121
Query: 478 IMFETFNSPA 507
I+FETFN+PA
Sbjct: 122 IIFETFNAPA 131
Score = 40.3 bits (90), Expect = 4e-04
Identities = 22/35 (62%), Positives = 23/35 (65%)
Frame = +3
Query: 576 DSGDGVSHTXPI*EGYALPXAHPPSGPWPGRDSTD 680
DSGDGV+HT PI EGYALP A GRD TD
Sbjct: 154 DSGDGVTHTVPIYEGYALPHAIMRL-DLAGRDLTD 187
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 142 bits (343), Expect = 8e-35
Identities = 66/129 (51%), Positives = 86/129 (66%), Gaps = 1/129 (0%)
Frame = +1
Query: 124 DVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAXXKRG 303
D + +DNGSG KAGFAGDD P+ +FP+ VGR +H+ VM QKD +VG EA RG
Sbjct: 8 DNQPICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFVGSEAQNLRG 67
Query: 304 ILTLKYPIEHGIITNWDDMEKIWHHTFYN-ELRVAPEEHPVLLTEAPLNPKANREKMTXI 480
+L ++ PIE GII NW DME+IW + + + +L PEEHP+LLTE PL N+EK+
Sbjct: 68 LLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEY 127
Query: 481 MFETFNSPA 507
+ET N PA
Sbjct: 128 FYETLNVPA 136
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 125 bits (302), Expect = 7e-30
Identities = 59/124 (47%), Positives = 84/124 (67%), Gaps = 2/124 (1%)
Frame = +1
Query: 130 AALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGVMVGMGQKDSYVGDEAXXKRG 303
A +V+DNG+G K G+A D+ PR FPSIVGRP R + + KD VGDEA R
Sbjct: 4 APIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAVRS 63
Query: 304 ILTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTXIM 483
+L +KYP+E+GII ++++M ++W +TF+ +L++ P +LLTE P+NP ANREKM M
Sbjct: 64 LLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPPMNPVANREKMCETM 123
Query: 484 FETF 495
FE +
Sbjct: 124 FERY 127
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 101 bits (243), Expect = 1e-22
Identities = 58/145 (40%), Positives = 77/145 (53%), Gaps = 24/145 (16%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVG---------MGQK-------- 264
+++DNG+G K G+AG+DAP VFP+++ R G G M K
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVIAT-RSAGASSGPAVSSKPSYMASKGSGHLSSK 66
Query: 265 ------DSYVGDEAXXKRGI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPV 423
D ++G++A K +L YPI HG I NWD ME+ W + + LR PE+H
Sbjct: 67 RATEDLDFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYF 126
Query: 424 LLTEAPLNPKANREKMTXIMFETFN 498
LLTE PLNP NRE IMFE+FN
Sbjct: 127 LLTEPPLNPPENRENTAEIMFESFN 151
Score = 32.7 bits (71), Expect = 0.073
Identities = 16/34 (47%), Positives = 21/34 (61%)
Frame = +3
Query: 576 DSGDGVSHTXPI*EGYALPXAHPPSGPWPGRDST 677
DSGDGV+H P+ EGY + + + P GRD T
Sbjct: 185 DSGDGVTHIIPVAEGYVI-GSSIKTMPLAGRDVT 217
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 84.2 bits (199), Expect = 2e-17
Identities = 45/135 (33%), Positives = 79/135 (58%), Gaps = 1/135 (0%)
Frame = +1
Query: 106 FKMCDDDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDE 285
F+ +D+ LV+DNGS +AG+ G+ P+ VF ++V R R + + + + VG++
Sbjct: 18 FQNVSNDIP-LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYRDRK----LSRTSTLVGND 72
Query: 286 AXXKRGILTL-KYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANR 462
+ G ++ + P E +I+NWD ME++ +TF +L + EHP+ +TE NP R
Sbjct: 73 TLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICMTEPLANPTYVR 131
Query: 463 EKMTXIMFETFNSPA 507
MT ++FE +N+P+
Sbjct: 132 STMTELLFELYNAPS 146
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 79.4 bits (187), Expect = 6e-16
Identities = 45/130 (34%), Positives = 67/130 (51%), Gaps = 1/130 (0%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAXXKR 300
D+V+A+V+D GS + GF+G+D P+ V PS G G++ G+E K
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGE-------FSDGRR--LFGEEYIYKS 59
Query: 301 GI-LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTX 477
+ +K I +G + NWD +W + +L+ P EHP+L+TE NP NR K
Sbjct: 60 NPGMEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLE 119
Query: 478 IMFETFNSPA 507
MFE+ PA
Sbjct: 120 TMFESLRCPA 129
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 66.9 bits (156), Expect = 4e-12
Identities = 37/131 (28%), Positives = 69/131 (52%), Gaps = 2/131 (1%)
Frame = +1
Query: 121 DDVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAXXKR 300
+++ +LV+D GS + G+AG+++P + PS + GV + ++ YV DE
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPS------YYGVRSDVTGRNKYVVDELQIHA 61
Query: 301 GI--LTLKYPIEHGIITNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMT 474
I + +K +GII +W+ W +L+V P E+ +++TE NP++ R+++
Sbjct: 62 PIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMITEPSWNPQSVRQQIM 121
Query: 475 XIMFETFNSPA 507
FE + PA
Sbjct: 122 EAAFEQLHVPA 132
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 33.9 bits (74), Expect = 0.032
Identities = 26/100 (26%), Positives = 34/100 (34%)
Frame = +1
Query: 577 TPEMVSPTPHPSKKVTLCPXPILXPDLGLVATRPTXLMXIPHPXGLXRSPPHPLTGXXXP 756
TP+ P P P P P L P + P + + PP P T P
Sbjct: 228 TPKQADPLPAPPPP----PPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPPPPATPSQPP 283
Query: 757 RTPTXXASWXXXXXXXXXHGAXATXSPPSHLPPEAKAXPN 876
R PT ++ H + SP S P +A PN
Sbjct: 284 RPPTNASTRSTGTSSSMAHSYDSPPSPSS--PSDAYGDPN 321
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 33.1 bits (72), Expect = 0.055
Identities = 31/134 (23%), Positives = 56/134 (41%), Gaps = 4/134 (2%)
Frame = +1
Query: 136 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAXXKRGILTL 315
+V+DNG+ KAGFAG P+ + R + G + ++G+E TL
Sbjct: 8 IVLDNGAYHIKAGFAGGKV--VEIPNCLTRSKD-------GNR-LFLGNELANCNDFTTL 57
Query: 316 KYPIEH--GIITNWDDMEKIWHHTFYNELRVAPE--EHPVLLTEAPLNPKANREKMTXIM 483
++ H G + +W +W N + P ++ +LLT+ + ++
Sbjct: 58 QFRRAHEKGYLVHWSTETAVWDLVMRNVGVMEPSMADYSLLLTQPVFTMPSIEHNTIQLV 117
Query: 484 FETFNSPAHVXRHP 525
FE F A++ P
Sbjct: 118 FEEFQFDAYLPCTP 131
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 29.5 bits (63), Expect = 0.68
Identities = 21/69 (30%), Positives = 25/69 (36%)
Frame = +1
Query: 574 PTPEMVSPTPHPSKKVTLCPXPILXPDLGLVATRPTXLMXIPHPXGLXRSPPHPLTGXXX 753
P P P P PS V P P P V T L +P P ++PP P
Sbjct: 1192 PPPSEAPPVPKPSVGVPPVPPPSTAPP---VPTPSAGLPPVPVPTA--KAPPVPAPSSEA 1246
Query: 754 PRTPTXXAS 780
P T +S
Sbjct: 1247 PSVSTPRSS 1255
Score = 27.5 bits (58), Expect = 2.7
Identities = 26/107 (24%), Positives = 30/107 (28%)
Frame = +1
Query: 574 PTPEMVSPTPHPSKKVTLCPXPILXPDLGLVATRPTXLMXIPHPXGLXRSPPHPLTGXXX 753
P P P P PS P P P V +P P G +PP P
Sbjct: 1115 PAPSGAPPVPKPSVAAPPVPVPSGAPP---VPKPSVAAPPVPAPSG---APPVPKPSVAA 1168
Query: 754 PRTPTXXASWXXXXXXXXXHGAXATXSPPSHLPPEAKAXPNLPSXAP 894
P P + PPS PP K +P P
Sbjct: 1169 PPVPAPSSG---IPPVPKPAAGVPPVPPPSEAPPVPKPSVGVPPVPP 1212
Score = 26.2 bits (55), Expect = 6.3
Identities = 26/114 (22%), Positives = 38/114 (33%), Gaps = 7/114 (6%)
Frame = +1
Query: 574 PTPEMVSPTPHPS------KKVTLCPXPILXPDLGLVATRPTXLM-XIPHPXGLXRSPPH 732
P P + P P PS K ++ P+ P +P+ +P P G +PP
Sbjct: 1086 PAPSGIPPVPKPSVAAPPVPKPSVAVPPVPAPSGAPPVPKPSVAAPPVPVPSG---APPV 1142
Query: 733 PLTGXXXPRTPTXXASWXXXXXXXXXHGAXATXSPPSHLPPEAKAXPNLPSXAP 894
P P P + A +P S +PP K +P P
Sbjct: 1143 PKPSVAAPPVPAPSGA---PPVPKPSVAAPPVPAPSSGIPPVPKPAAGVPPVPP 1193
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 29.1 bits (62), Expect = 0.90
Identities = 14/43 (32%), Positives = 25/43 (58%), Gaps = 2/43 (4%)
Frame = +1
Query: 382 FYNELRVAPEEHPVLLTE--APLNPKANREKMTXIMFETFNSP 504
+Y L E+HP+LLT+ A L P+ + ++ I ++ N+P
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTP 1441
>SPAC56F8.02 |||AMP binding enzyme |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1517
Score = 28.7 bits (61), Expect = 1.2
Identities = 15/48 (31%), Positives = 25/48 (52%)
Frame = +1
Query: 181 GDDAPRAVFPSIVGRPRHQGVMVGMGQKDSYVGDEAXXKRGILTLKYP 324
G+ PRA F ++ P H G+++ M KD G+E +G + + P
Sbjct: 502 GNQNPRATFVPLLCLPEHGGMVISM--KDWIGGEEFMSPKGFKSPRTP 547
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/65 (27%), Positives = 28/65 (43%), Gaps = 4/65 (6%)
Frame = +1
Query: 322 PIEHGIITNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTXIMFE 489
PI+ G + +W+ ++ W H Y+ L P + +PV L +RE T FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 490 TFNSP 504
P
Sbjct: 172 ECQVP 176
>SPAC688.12c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 167
Score = 27.1 bits (57), Expect = 3.6
Identities = 15/59 (25%), Positives = 26/59 (44%)
Frame = +1
Query: 400 VAPEEHPVLLTEAPLNPKANREKMTXIMFETFNSPAHVXRHPXRALAVXLRSYHRYXAP 576
++ EE P LT+ NPK N+ + + F + H+ + + R+YH P
Sbjct: 1 MSEEESPYQLTKTFSNPKNNKIGLAIFLIGAFINLIHIYKPKGPSNNPTKRNYHISFGP 59
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,716,791
Number of Sequences: 5004
Number of extensions: 50504
Number of successful extensions: 173
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 145
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 167
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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