BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_O21
(1004 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 36 0.009
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 36 0.009
SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr... 31 0.19
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 31 0.26
SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex p... 29 1.4
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 1.4
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 28 2.4
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 27 3.2
SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces pomb... 26 7.3
SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr 2|||M... 26 7.3
SPBC1289.12 |usp109||U1 snRNP-associated protein Usp109|Schizosa... 26 9.6
SPAC821.08c |slp1||sleepy homolog Slp1|Schizosaccharomyces pombe... 26 9.6
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 35.9 bits (79), Expect = 0.009
Identities = 22/62 (35%), Positives = 24/62 (38%), Gaps = 1/62 (1%)
Frame = +1
Query: 607 PPLPPXVXSXLPPXXXPXLXSPFXTXPNXPXLPLPPXXPPLXRXLXHAXXAPLSXP-PPX 783
P LPP LPP P L P P P P PPL + A P + P PP
Sbjct: 421 PSLPPSAPPSLPPSAPPSL--PMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPP 478
Query: 784 XP 789
P
Sbjct: 479 AP 480
Score = 34.7 bits (76), Expect = 0.021
Identities = 20/63 (31%), Positives = 23/63 (36%), Gaps = 2/63 (3%)
Frame = +1
Query: 607 PPLPPXVXSXL--PPXXXPXLXSPFXTXPNXPXLPLPPXXPPLXRXLXHAXXAPLSXPPP 780
PP+PP + PP P +P P PP PP R A A PPP
Sbjct: 254 PPIPPPSNGTVSSPPNSPPRPIAPVSMNPAINSTSKPPLPPPSSRVSAAALAANKKRPPP 313
Query: 781 XXP 789
P
Sbjct: 314 PPP 316
Score = 33.5 bits (73), Expect = 0.048
Identities = 27/107 (25%), Positives = 31/107 (28%)
Frame = +1
Query: 607 PPLPPXVXSXLPPXXXPXLXSPFXTXPNXPXLPLPPXXPPLXRXLXHAXXAPLSXPPPXX 786
PP PP P L + T + P +P PP PP AP S PP
Sbjct: 388 PPAPPPAIPGRSAPALPPLGNASRT--STPPVPTPPSLPP---------SAPPSLPPSAP 436
Query: 787 PXXXXXXXXXXXXXXXXXXXXXXXRXQXPCHPXPPXSPXPTXPPTPA 927
P P PP +P P P PA
Sbjct: 437 PSLPMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPA 483
Score = 31.5 bits (68), Expect = 0.19
Identities = 15/43 (34%), Positives = 20/43 (46%), Gaps = 3/43 (6%)
Frame = +1
Query: 607 PPLPPXVXSXLPPXXXPXLXSPF---XTXPNXPXLPLPPXXPP 726
PP+PP + S PP P L +P P+ + PP PP
Sbjct: 230 PPIPPSIPSSRPPERVPSLSAPAPPPIPPPSNGTVSSPPNSPP 272
Score = 27.1 bits (57), Expect = 4.2
Identities = 10/20 (50%), Positives = 10/20 (50%)
Frame = +2
Query: 545 PXXPPPPPXPXPXPXPRPXA 604
P PP P P P P P P A
Sbjct: 467 PPLPPAAPAPPPAPAPAPAA 486
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 35.9 bits (79), Expect = 0.009
Identities = 20/61 (32%), Positives = 28/61 (45%), Gaps = 1/61 (1%)
Frame = +1
Query: 601 RXPPLPPXVXSXLPPXXXPXLXSPFXTXPNX-PXLPLPPXXPPLXRXLXHAXXAPLSXPP 777
R PP+P + S PP P +P P+ P +P+P PP+ + A AP P
Sbjct: 1010 RVPPVPK-LSSKAPPVPLPSADAPPIPVPSTAPPVPIPTSTPPVPKSSSGAPSAPPPVPA 1068
Query: 778 P 780
P
Sbjct: 1069 P 1069
>SPBC2D10.10c |fib1|fib|fibrillarin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 305
Score = 31.5 bits (68), Expect = 0.19
Identities = 18/42 (42%), Positives = 19/42 (45%), Gaps = 1/42 (2%)
Frame = -1
Query: 728 RGGXXGGRGSXGXLGXVXKGDXRXGLXXG-GXXEXTXGGRGG 606
RGG GGRG G +G R G G G GGRGG
Sbjct: 18 RGGFNGGRGGFGGGRGGARGGGRGGARGGRGGRGGARGGRGG 59
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 31.1 bits (67), Expect = 0.26
Identities = 13/36 (36%), Positives = 15/36 (41%)
Frame = +2
Query: 554 PPPPPXPXPXPXPRPXAXPLYXRGFXLXSPPXXXPP 661
PPPPP P P P P+ + PP PP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPP 767
Score = 27.5 bits (58), Expect = 3.2
Identities = 16/48 (33%), Positives = 16/48 (33%), Gaps = 4/48 (8%)
Frame = +2
Query: 530 PXQPXPXXPPPPPXPXPXPXPRP----XAXPLYXRGFXLXSPPXXXPP 661
P P P P P P P P P P P G PP PP
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPP 781
Score = 25.8 bits (54), Expect = 9.6
Identities = 19/58 (32%), Positives = 23/58 (39%)
Frame = +1
Query: 607 PPLPPXVXSXLPPXXXPXLXSPFXTXPNXPXLPLPPXXPPLXRXLXHAXXAPLSXPPP 780
PP PP V + P P +P P P + PP PP + A P PPP
Sbjct: 733 PPPPPAV---IVPTPAP---APIPVPPPAPIMGGPPPPPP-PPGVAGAGPPPPPPPPP 783
>SPBC20F10.01 |gar1|SPBC25H2.01c|snoRNP pseudouridylase complex
protein Gar1 |Schizosaccharomyces pombe|chr 2|||Manual
Length = 194
Score = 28.7 bits (61), Expect = 1.4
Identities = 16/43 (37%), Positives = 17/43 (39%)
Frame = -1
Query: 728 RGGXXGGRGSXGXLGXVXKGDXRXGLXXGGXXEXTXGGRGGXR 600
RGG G RG G G+ R G G G RGG R
Sbjct: 137 RGGRGGFRGGRGGSRGGFGGNSRGGFGGGSRGGFGGGSRGGSR 179
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 7.3
Identities = 12/41 (29%), Positives = 13/41 (31%)
Frame = +2
Query: 539 PXPXXPPPPPXPXPXPXPRPXAXPLYXRGFXLXSPPXXXPP 661
P P PPPPP P P+ R PP
Sbjct: 234 PLPAPPPPPPPTLPPQSTNTSQLPMPSRNVNNLGSQVNIPP 274
Score = 24.6 bits (51), Expect(2) = 3.9
Identities = 9/19 (47%), Positives = 9/19 (47%)
Frame = +2
Query: 530 PXQPXPXXPPPPPXPXPXP 586
P Q P PPPP P P
Sbjct: 229 PKQADPLPAPPPPPPPTLP 247
Score = 23.8 bits (49), Expect(2) = 1.4
Identities = 8/13 (61%), Positives = 8/13 (61%)
Frame = +1
Query: 880 PXPPXSPXPTXPP 918
P PP P PT PP
Sbjct: 236 PAPPPPPPPTLPP 248
Score = 23.0 bits (47), Expect(2) = 1.4
Identities = 11/41 (26%), Positives = 16/41 (39%)
Frame = +1
Query: 667 SPFXTXPNXPXLPLPPXXPPLXRXLXHAXXAPLSXPPPXXP 789
+P + PN P P PP + + + PPP P
Sbjct: 155 APTVSAPNSMVSPPPSFQPPSAAAPATSLPSDYNPPPPPPP 195
Score = 20.6 bits (41), Expect(2) = 3.9
Identities = 7/13 (53%), Positives = 7/13 (53%)
Frame = +2
Query: 560 PPPXPXPXPXPRP 598
PPP P PRP
Sbjct: 273 PPPPATPSQPPRP 285
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 27.9 bits (59), Expect = 2.4
Identities = 13/31 (41%), Positives = 14/31 (45%)
Frame = +2
Query: 530 PXQPXPXXPPPPPXPXPXPXPRPXAXPLYXR 622
P P P PPPPP P P P P Y +
Sbjct: 6 PGNPPP--PPPPPGFEPPSQPPPPPPPGYVK 34
Score = 25.8 bits (54), Expect = 9.6
Identities = 10/24 (41%), Positives = 11/24 (45%)
Frame = +2
Query: 539 PXPXXPPPPPXPXPXPXPRPXAXP 610
P PPPPP P P +P P
Sbjct: 5 PPGNPPPPPPPPGFEPPSQPPPPP 28
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 27.5 bits (58), Expect = 3.2
Identities = 11/24 (45%), Positives = 11/24 (45%)
Frame = +2
Query: 539 PXPXXPPPPPXPXPXPXPRPXAXP 610
P P PPPP P P P A P
Sbjct: 1709 PPPMSVPPPPSAPPMPAGPPSAPP 1732
Score = 25.8 bits (54), Expect = 9.6
Identities = 10/23 (43%), Positives = 10/23 (43%)
Frame = +2
Query: 530 PXQPXPXXPPPPPXPXPXPXPRP 598
P Q PPPPP P P P
Sbjct: 1699 PPQMSAPTPPPPPMSVPPPPSAP 1721
>SPAC2F3.14c |||conserved fungal protein|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 331
Score = 26.2 bits (55), Expect = 7.3
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = +2
Query: 530 PXQPXPXXPPPPPXPXPXPXPRPXAXPL 613
P +P P PP P P P P P PL
Sbjct: 115 PEEPLPGEPPLPDEPVP-EEPLPGEPPL 141
Score = 25.8 bits (54), Expect = 9.6
Identities = 12/28 (42%), Positives = 13/28 (46%)
Frame = +2
Query: 530 PXQPXPXXPPPPPXPXPXPXPRPXAXPL 613
P +P P PP P P P P P PL
Sbjct: 99 PEEPLPREPPLPNEPVP-EEPLPGEPPL 125
>SPBC13E7.09 |vrp1||verprolin|Schizosaccharomyces pombe|chr
2|||Manual
Length = 309
Score = 26.2 bits (55), Expect = 7.3
Identities = 10/19 (52%), Positives = 10/19 (52%)
Frame = +2
Query: 557 PPPPXPXPXPXPRPXAXPL 613
PPPP P P P A PL
Sbjct: 5 PPPPPPAPAPAAAAPAPPL 23
>SPBC1289.12 |usp109||U1 snRNP-associated protein
Usp109|Schizosaccharomyces pombe|chr 2|||Manual
Length = 352
Score = 25.8 bits (54), Expect = 9.6
Identities = 16/54 (29%), Positives = 18/54 (33%)
Frame = +1
Query: 613 LPPXVXSXLPPXXXPXLXSPFXTXPNXPXLPLPPXXPPLXRXLXHAXXAPLSXP 774
LP + P P S PN P P P P + H APL P
Sbjct: 252 LPSKQSTYWPALAAPVYPS-MKDVPNNPFTPFSPINPYYAKSWNHTASAPLLPP 304
>SPAC821.08c |slp1||sleepy homolog Slp1|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 488
Score = 25.8 bits (54), Expect = 9.6
Identities = 13/27 (48%), Positives = 16/27 (59%)
Frame = +1
Query: 151 ILSSRLRSGAGFEHDQRCSRAQVEDLQ 231
+LSS RSGA HD R + Q+ LQ
Sbjct: 275 VLSSGSRSGAIHHHDVRIANHQIGTLQ 301
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,110,447
Number of Sequences: 5004
Number of extensions: 33241
Number of successful extensions: 251
Number of sequences better than 10.0: 12
Number of HSP's better than 10.0 without gapping: 92
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 188
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 521265546
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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