Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_L07
(883 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 176 4e-45
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 114 1e-26
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 99 4e-22
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 87 3e-18
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 72 1e-13
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 52 8e-08
SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces pombe... 40 6e-04
SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein Hsp60... 29 1.2
SPBC32F12.10 |||phosphoglucomutase |Schizosaccharomyces pombe|ch... 27 2.7
SPBC32F12.02 |rec14||recombination protein Rec14|Schizosaccharom... 27 2.7
SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|ch... 27 3.5
SPBC8D2.12c |||mitochondrial DNA binding protein |Schizosaccharo... 27 4.7
SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl modifica... 26 6.2
SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces pomb... 26 6.2
SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces... 26 8.2
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 176 bits (428), Expect = 4e-45
Identities = 84/204 (41%), Positives = 121/204 (59%), Gaps = 1/204 (0%)
Frame = +2
Query: 89 AAVLHGANDVRIEKIPVPEINDD-EVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIG 265
A VL D IE P + DD +V + I GICGSDV + G G ++ KP+++G
Sbjct: 7 AFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILKKPMILG 66
Query: 266 HEGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNL 445
HE AG VV+VG VSSL+ GD VA+EP CR C+ C+ G+YNLC + ++ G L
Sbjct: 67 HESAGVVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPPYDGTL 126
Query: 446 CRYYKHVADFCHKLPDNLTMEEGAAVQPLAIVIHACNRAKITLGSKIVILGAGPIGILCA 625
YY DFC KLP +++EEGA +P+++ +HA R + GS+++++G G +G+L
Sbjct: 127 RTYYITTEDFCTKLPKQISVEEGALFEPMSVAVHAMTRGNLKCGSRVLVMGCGTVGLLMM 186
Query: 626 MSAKAMGASKIILTDVVQSRLDAA 697
AKA GA I+ D SR++ A
Sbjct: 187 AVAKAYGAIDIVAVDASPSRVEFA 210
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 114 bits (275), Expect = 1e-26
Identities = 87/268 (32%), Positives = 133/268 (49%), Gaps = 24/268 (8%)
Frame = +2
Query: 89 AAVLHGANDVRIEKIPVPEI-NDDEVLIKIDCVGIC-GSDVKLYSTGTCGADVIDKPIVI 262
A V G +V+I ++P P I + +V++K IC GSD ++S G I+K ++
Sbjct: 39 ACVWDGPLNVKIAEVPKPTITHPKDVIVKTTACTICSGSDSHIFSGEMPG---IEKGAIL 95
Query: 263 GHEGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLC--------VEPRYC 418
GHE G V + GD+V++L +GDRV I C C CKR +Y C ++ Y
Sbjct: 96 GHESCGIVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAACDTTNDSKLMDVNYG 155
Query: 419 SSMGA-----------PGNLCRYYK-HVADF-CHKLPDNLTMEEGAAVQP-LAIVIHACN 556
S A PG Y + A+ C KLPD++ EG + L +HAC
Sbjct: 156 SHHSAIFGYTKLLGDVPGCQAEYIRVPFAEINCCKLPDDIPDSEGLFMSDVLCTSLHACT 215
Query: 557 RAKITLGSKIVILGAGPIGILCAMSAKAMGASKIILTDVVQSRLDAALELGADNVLLVRR 736
++ G + I G GPIG+ A+ +GASK+I +VV R++ A + V + R
Sbjct: 216 LGEVKKGDTVAIWGMGPIGLYAGRWAQILGASKVIGIEVVPERIELARQKFGFTV-IDRN 274
Query: 737 EYTDEEVVXKIVXLLGDRPDVSIDACGY 820
E +D V KI+ L+ + D +I+A G+
Sbjct: 275 EVSD--VPKKIMELVSNGVDCAIEASGF 300
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 99 bits (238), Expect = 4e-22
Identities = 71/224 (31%), Positives = 110/224 (49%), Gaps = 7/224 (3%)
Frame = +2
Query: 89 AAVLH---GANDVRIEKIPVPEINDDEVLIKIDCVGICGSDVK-LYSTGTCGADVIDKPI 256
AAV H G +V+ E++PV E DEVL+ I G+C +D+ L A + P+
Sbjct: 9 AAVFHTHGGPENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQGDWPLPAKM---PL 65
Query: 257 VIGHEGAGTVVKVGDKVSSLRVGDRVAIE-PTQPCRSCELCKRGKYNLCVEPRYCSSMGA 433
+ GHEGAG VVKVG V+ L++GDRV ++ C +CE C + + +C + S
Sbjct: 66 IGGHEGAGVVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICPHIQ-LSGYTV 124
Query: 434 PGNLCRYYKHVADFCHKLPDNLTMEEGAAVQPLAIVIH-ACNRAKITLGSKIVILGA-GP 607
G Y A +P+++ +E A + I + A +K+ G I I GA G
Sbjct: 125 DGTFQHYCIANATHATIIPESVPLEVAAPIMCAGITCYRALKESKVGPGEWICIPGAGGG 184
Query: 608 IGILCAMSAKAMGASKIILTDVVQSRLDAALELGADNVLLVRRE 739
+G L AKAM A +++ D + + GA+ L ++E
Sbjct: 185 LGHLAVQYAKAM-AMRVVAIDTGDDKAELVKSFGAEVFLDFKKE 227
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 87.0 bits (206), Expect = 3e-18
Identities = 72/236 (30%), Positives = 112/236 (47%), Gaps = 24/236 (10%)
Frame = +2
Query: 89 AAVLHGAND-VRIEKIPVPEINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDK-PIVI 262
AAV GA + + IE I V EV +K+D +C +D S G D PIV+
Sbjct: 12 AAVAWGAKEPLSIEDIQVAPPKAHEVRVKVDWSAVCHTDAYTLS----GVDPEGAFPIVL 67
Query: 263 GHEGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMG-APG 439
GHEGAG V +G+ V ++R GD V + T C+ C+ C+ GK NLC + R G P
Sbjct: 68 GHEGAGIVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLCSKIRETQGRGLMPD 127
Query: 440 NL----CR-----YYKHVADFC-HKLPDNLTMEEGAAVQPL-AIVIHAC----------N 556
CR +Y + F + + ++++ + PL +I + C +
Sbjct: 128 GTSRFSCRDKTLLHYMGCSSFSQYTVVADISLVAISHSAPLRSICLLGCGVTTGFGAVTH 187
Query: 557 RAKITLGSKIVILGAGPIGILCAMSAKAMGASKIILTDVVQSRLDAALELGADNVL 724
AK+ GS + ++G G +G+ A A GAS+II D+ + A + GA + +
Sbjct: 188 SAKVESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAKKFGATDFI 243
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 71.7 bits (168), Expect = 1e-13
Identities = 66/246 (26%), Positives = 106/246 (43%), Gaps = 23/246 (9%)
Frame = +2
Query: 56 KLTGCKLXENYAAVLHGANDVRIEKIPVPEINDDEVLIKIDCVGICGSDVKLYSTGTCGA 235
K+ CK A A + IE + V EV IKI G+C +D S G
Sbjct: 9 KIINCKA----AVAWQPAAPLSIENVQVFPPRVHEVRIKIVNSGVCHTDAYTLS----GK 60
Query: 236 DVIDK-PIVIGHEGAGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLC---- 400
D P+++GHEGAG V VG +V++++VGD V T C++C+ CK GK NLC
Sbjct: 61 DPEGLFPVILGHEGAGIVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLCGRIR 120
Query: 401 ------VEPRYCSSMGAPGNLCRYYKHVADFC-HKLPDNLTMEEGAAVQPL-AIVIHACN 556
+ P S GN ++ + F + + ++++ + PL ++ + C
Sbjct: 121 TTQGKGLMPDGTSRFSCNGNTLLHFMGCSTFSEYTVVADISVVAIERLAPLDSVCLLGCG 180
Query: 557 ----------RAKITLGSKIVILGAGPIGILCAMSAKAMGASKIILTDVVQSRLDAALEL 706
A I G + + G G +G+ A A +I DV + + A+
Sbjct: 181 ITTGYGAATITADIKEGDSVAVFGLGSVGLAVIQGAVKKRAGRIFGIDVNPEKKNWAMSF 240
Query: 707 GADNVL 724
GA + +
Sbjct: 241 GATDFI 246
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 52.4 bits (120), Expect = 8e-08
Identities = 52/217 (23%), Positives = 95/217 (43%), Gaps = 8/217 (3%)
Frame = +2
Query: 98 LHGANDVRIEKIPVPE-INDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIGHEG 274
+ G + ++ E+ VP+ +N EVL+K+ + D+ + + G + P+V G +G
Sbjct: 11 ISGFDQLKPEEYEVPQKLNPGEVLVKLKAASLNYRDL-IITKGLYPLP-LQLPVVPGSDG 68
Query: 275 AGTVVKVGDKVSSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNLCRY 454
AG + KVG+ V GD V C + S++G + C +
Sbjct: 69 AGIIEKVGEDVEGFEKGDSVV---------CNFFTNYLDGTPTDFATHSALGGTRDGC-F 118
Query: 455 YKHVADFCHKL---PDNLTMEEGAAVQPLAIV----IHACNRAKITLGSKIVILGAGPIG 613
K+ H L P NL+ EE A + A+ + ++ G+ +++LG G +
Sbjct: 119 QKYAVLPAHALVHAPKNLSFEEIATLPCAAVTAWNGLFGSKEHQVKPGNNVLVLGTGGVS 178
Query: 614 ILCAMSAKAMGASKIILTDVVQSRLDAALELGADNVL 724
A A GA+ + +T +L+ A +LGA + +
Sbjct: 179 TFALQFALAAGAN-VTVTSSSDEKLEFAKKLGATHTI 214
>SPBC337.11 |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 325
Score = 39.5 bits (88), Expect = 6e-04
Identities = 22/73 (30%), Positives = 39/73 (53%)
Frame = +2
Query: 104 GANDVRIEKIPVPEINDDEVLIKIDCVGICGSDVKLYSTGTCGADVIDKPIVIGHEGAGT 283
G + I+ +P+P+ + E+L+KI+ I SD+ + +TG G P ++G + AGT
Sbjct: 19 GCLGIEIQSVPIPQPKNGELLVKIEAAAINPSDL-MNATG--GFPYTVYPRIVGRDYAGT 75
Query: 284 VVKVGDKVSSLRV 322
V+ + RV
Sbjct: 76 VISGASHLVGTRV 88
>SPAC12G12.04 |hsp60|hsp60|mitochondrial heat shock protein
Hsp60|Schizosaccharomyces pombe|chr 1|||Manual
Length = 582
Score = 28.7 bits (61), Expect = 1.2
Identities = 13/33 (39%), Positives = 19/33 (57%)
Frame = +2
Query: 221 GTCGADVIDKPIVIGHEGAGTVVKVGDKVSSLR 319
G+CG+ + K I +GAG VKV D+ +R
Sbjct: 349 GSCGSVTVTKEDTIIMKGAGDHVKVNDRCEQIR 381
>SPBC32F12.10 |||phosphoglucomutase |Schizosaccharomyces pombe|chr
2|||Manual
Length = 554
Score = 27.5 bits (58), Expect = 2.7
Identities = 13/34 (38%), Positives = 19/34 (55%), Gaps = 1/34 (2%)
Frame = +2
Query: 404 EPRYCSSMGAPGNLCRYYKHVADFCHKLPD-NLT 502
E +C +G P ++C+ K + DF PD NLT
Sbjct: 224 EALFCKELGMPSSVCQNCKPLPDFGGGHPDPNLT 257
>SPBC32F12.02 |rec14||recombination protein
Rec14|Schizosaccharomyces pombe|chr 2|||Manual
Length = 302
Score = 27.5 bits (58), Expect = 2.7
Identities = 21/96 (21%), Positives = 39/96 (40%), Gaps = 5/96 (5%)
Frame = +2
Query: 296 GDKVSSLRVGDRVAI-EPTQPCRSCELCKRGKYNLCVE----PRYCSSMGAPGNLCRYYK 460
G ++ V R+A+ + C+ EL +GK+ LC++ R+ S G L
Sbjct: 115 GQTIAFTSVDGRIAVWDNPSDCKISELDTKGKFGLCIDYSPNGRFIVSGHQTGQLFLIST 174
Query: 461 HVADFCHKLPDNLTMEEGAAVQPLAIVIHACNRAKI 568
H L + + A P + ++ A +K+
Sbjct: 175 ETGRLFHVLSGHTSPVRSVAFSPGSTLLAAAGDSKM 210
>SPBC530.08 |||transcription factor |Schizosaccharomyces pombe|chr
2|||Manual
Length = 815
Score = 27.1 bits (57), Expect = 3.5
Identities = 15/50 (30%), Positives = 24/50 (48%)
Frame = +2
Query: 308 SSLRVGDRVAIEPTQPCRSCELCKRGKYNLCVEPRYCSSMGAPGNLCRYY 457
S + G +VA + R+C++C+R K C R C + A C Y+
Sbjct: 12 SKILEGAKVATRRRRVTRACDMCRRKKIK-CDGLRPCKNCKAGKLECTYH 60
>SPBC8D2.12c |||mitochondrial DNA binding protein
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 293
Score = 26.6 bits (56), Expect = 4.7
Identities = 11/23 (47%), Positives = 17/23 (73%)
Frame = +2
Query: 686 LDAALELGADNVLLVRREYTDEE 754
LD A+E GA++++ +EY DEE
Sbjct: 185 LDDAIEAGAEDIVNRPKEYIDEE 207
>SPBC30B4.06c |||tRNA uridine 5-carboxymethylaminomethyl
modification enzyme|Schizosaccharomyces pombe|chr
2|||Manual
Length = 666
Score = 26.2 bits (55), Expect = 6.2
Identities = 10/28 (35%), Positives = 17/28 (60%)
Frame = +2
Query: 584 IVILGAGPIGILCAMSAKAMGASKIILT 667
+V++G G G+ A +A +GA +LT
Sbjct: 19 VVVIGGGHAGVEAAAAASRLGAKTTLLT 46
>SPAC186.08c |||L-lactate dehydrogenase |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 330
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/44 (34%), Positives = 28/44 (63%), Gaps = 2/44 (4%)
Frame = +2
Query: 581 KIVILGAGPIGILCAMSAKAMG-ASKIILTDVVQSRLDA-ALEL 706
KIVI+GAG +G A + G A++I++ D+ + + + A++L
Sbjct: 22 KIVIVGAGNVGSTTAFTLLLSGLAAEIVIIDLNKKKAEGEAMDL 65
>SPCC584.05 |sec1||SNARE binding protein Sec1|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 693
Score = 25.8 bits (54), Expect = 8.2
Identities = 13/28 (46%), Positives = 18/28 (64%)
Frame = +3
Query: 54 RS*RVASXPRTTLLCYTEPTTSESRKFQ 137
R+ R+AS RT + Y + T+ ESR FQ
Sbjct: 113 RTSRIASKIRTVQVAYLDFTSLESRYFQ 140
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,065,384
Number of Sequences: 5004
Number of extensions: 58544
Number of successful extensions: 196
Number of sequences better than 10.0: 15
Number of HSP's better than 10.0 without gapping: 190
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 442483990
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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