Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_K23
(970 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr 3|||... 38 0.002
SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, wit... 33 0.060
SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr 1... 33 0.080
SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit Spp42|Schizosac... 32 0.11
SPAC29B12.07 |sec16||multidomain vesicle coat component Sec16|Sc... 31 0.32
SPBC660.06 |||conserved fungal protein|Schizosaccharomyces pombe... 30 0.56
SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual 27 4.0
>SPCC895.05 |for3||formin For3|Schizosaccharomyces pombe|chr
3|||Manual
Length = 1461
Score = 38.3 bits (85), Expect = 0.002
Identities = 19/49 (38%), Positives = 19/49 (38%), Gaps = 4/49 (8%)
Frame = +2
Query: 452 PPPPRXXFXXPGGGGGGXXXPQKX----PPPPPXXGXPXGGPPPPXPXP 586
PPPP P P PPPPP G GPPPP P P
Sbjct: 734 PPPPAVIVPTPAPAPIPVPPPAPIMGGPPPPPPPPGVAGAGPPPPPPPP 782
Score = 27.1 bits (57), Expect = 4.0
Identities = 13/33 (39%), Positives = 13/33 (39%), Gaps = 4/33 (12%)
Frame = +1
Query: 451 PPPPPXXFXXPXGGGGGXXXPPKXP----PPPP 537
PPPPP P PP P PPPP
Sbjct: 732 PPPPPPAVIVPTPAPAPIPVPPPAPIMGGPPPP 764
Score = 27.1 bits (57), Expect = 4.0
Identities = 19/54 (35%), Positives = 19/54 (35%)
Frame = +2
Query: 425 PXXGGXKXXPPPPRXXFXXPGGGGGGXXXPQKXPPPPPXXGXPXGGPPPPXPXP 586
P GG PPPP G G P PPPPP GG P P
Sbjct: 756 PIMGGPPPPPPPP---------GVAGAGPP---PPPPPPPAVSAGGSRYYAPAP 797
Score = 26.6 bits (56), Expect = 5.3
Identities = 16/49 (32%), Positives = 16/49 (32%)
Frame = -2
Query: 561 PPXGXPXXGGGGGXFWGXXXPPPPPPGXXKXXRGGGGXXFXPPXXGPXP 415
PP P G G PPPPPP GG P P P
Sbjct: 763 PPPPPPGVAGAG--------PPPPPPPPPAVSAGGSRYYAPAPQAEPEP 803
>SPAC25G10.09c ||SPAC27F1.01c|actin cortical patch component, with EF
hand and WH2 motif |Schizosaccharomyces pombe|chr
1|||Manual
Length = 1794
Score = 33.1 bits (72), Expect = 0.060
Identities = 14/35 (40%), Positives = 14/35 (40%)
Frame = +1
Query: 451 PPPPPXXFXXPXGGGGGXXXPPKXPPPPPXGXXAP 555
PPPPP P PP PPPP AP
Sbjct: 1707 PPPPPMSVPPPPSAPPMPAGPPSAPPPPLPASSAP 1741
Score = 29.5 bits (63), Expect = 0.75
Identities = 12/26 (46%), Positives = 12/26 (46%), Gaps = 3/26 (11%)
Frame = +2
Query: 512 PQKXPPP---PPXXGXPXGGPPPPXP 580
P PPP PP P PPPP P
Sbjct: 1711 PMSVPPPPSAPPMPAGPPSAPPPPLP 1736
Score = 26.2 bits (55), Expect = 6.9
Identities = 11/30 (36%), Positives = 11/30 (36%)
Frame = +3
Query: 447 PXPPPPXXFFXPXGGGGGGXXXPKKXPPPP 536
P PPPP P P PPPP
Sbjct: 1705 PTPPPPPMSVPPPPSAPPMPAGPPSAPPPP 1734
>SPAC4F10.15c |wsp1||WASp homolog|Schizosaccharomyces pombe|chr
1|||Manual
Length = 574
Score = 32.7 bits (71), Expect = 0.080
Identities = 21/62 (33%), Positives = 21/62 (33%), Gaps = 3/62 (4%)
Frame = +2
Query: 398 RGXXGXGXGPXXGGXKXXPPPPRXXFXXPGGGGGGXXXPQ---KXPPPPPXXGXPXGGPP 568
RG G G PPPPR G PQ PPPPP G P
Sbjct: 322 RGKPPIGNGSSNSSLPPPPPPPRS-----NAAGSIPLPPQGRSAPPPPPPRSAPSTGRQP 376
Query: 569 PP 574
PP
Sbjct: 377 PP 378
Score = 31.1 bits (67), Expect = 0.24
Identities = 15/41 (36%), Positives = 15/41 (36%)
Frame = +2
Query: 452 PPPPRXXFXXPGGGGGGXXXPQKXPPPPPXXGXPXGGPPPP 574
PPP R P G G PPPPP P PP
Sbjct: 315 PPPSRRNRGKPPIGNGSSNSSLPPPPPPPRSNAAGSIPLPP 355
Score = 28.7 bits (61), Expect = 1.3
Identities = 16/45 (35%), Positives = 16/45 (35%), Gaps = 2/45 (4%)
Frame = +1
Query: 427 PXGGXKXXPPPP--PXXFXXPXGGGGGXXXPPKXPPPPPXGXXAP 555
P G P PP P P G PP P PPP AP
Sbjct: 440 PMGAPAAPPLPPSAPIAPPLPAGMPAAPPLPPAAPAPPPAPAPAP 484
>SPAC4F8.12c |spp42|cwf6|U5 snRNP complex subunit
Spp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 2363
Score = 32.3 bits (70), Expect = 0.11
Identities = 12/25 (48%), Positives = 12/25 (48%)
Frame = +2
Query: 512 PQKXPPPPPXXGXPXGGPPPPXPXP 586
P PPPPP G PPP P P
Sbjct: 6 PGNPPPPPPPPGFEPPSQPPPPPPP 30
Score = 27.1 bits (57), Expect = 4.0
Identities = 16/44 (36%), Positives = 18/44 (40%)
Frame = -2
Query: 513 GXXXPPPPPPGXXKXXRGGGGXXFXPPXXGPXPXPXXPLFXKKN 382
G PPPPPPG F PP P P P + +KN
Sbjct: 7 GNPPPPPPPPG------------FEPPSQPPPPPPPGYVKKRKN 38
Score = 26.6 bits (56), Expect = 5.3
Identities = 14/36 (38%), Positives = 15/36 (41%)
Frame = +1
Query: 451 PPPPPXXFXXPXGGGGGXXXPPKXPPPPPXGXXAPR 558
PPPPP G P + PPPPP G R
Sbjct: 10 PPPPPPP---------GFEPPSQPPPPPPPGYVKKR 36
Score = 25.8 bits (54), Expect = 9.2
Identities = 9/17 (52%), Positives = 9/17 (52%)
Frame = +1
Query: 916 PXPPXPPPHXTPXPXPP 966
P PP PPP P PP
Sbjct: 9 PPPPPPPPGFEPPSQPP 25
>SPAC29B12.07 |sec16||multidomain vesicle coat component
Sec16|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1995
Score = 30.7 bits (66), Expect = 0.32
Identities = 12/28 (42%), Positives = 12/28 (42%)
Frame = +2
Query: 497 GGXXXPQKXPPPPPXXGXPXGGPPPPXP 580
G Q PPPPP P GPP P
Sbjct: 1873 GSDLSNQAAPPPPPPMALPKAGPPSAAP 1900
Score = 25.8 bits (54), Expect = 9.2
Identities = 15/48 (31%), Positives = 15/48 (31%), Gaps = 2/48 (4%)
Frame = +2
Query: 455 PPPRXXFXXPGGGGGGXXXPQKXPPPPPXXGXP--XGGPPPPXPXPXT 592
PPP P G PP P G G P P P P T
Sbjct: 1882 PPPPPPMALPKAGPPSAAPTSALPPAGPPAGATAISGNPGMPAPVPLT 1929
>SPBC660.06 |||conserved fungal protein|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 273
Score = 29.9 bits (64), Expect = 0.56
Identities = 16/38 (42%), Positives = 17/38 (44%)
Frame = +1
Query: 385 LFXKKGXXGXGXGXPXGGXKXXPPPPPXXFXXPXGGGG 498
LF +G G G G GG PPP P F G GG
Sbjct: 179 LFHHRGHNGGGFGG-FGGGSGGPPPGPGGFGGFGGFGG 215
Score = 29.9 bits (64), Expect = 0.56
Identities = 14/29 (48%), Positives = 14/29 (48%)
Frame = -2
Query: 537 GGGGGXFWGXXXPPPPPPGXXKXXRGGGG 451
GGG G F G PPP PG G GG
Sbjct: 187 GGGFGGFGGGSGGPPPGPGGFGGFGGFGG 215
Score = 29.1 bits (62), Expect = 0.98
Identities = 14/30 (46%), Positives = 14/30 (46%)
Frame = -1
Query: 535 GGGGXFLGXXXPPPPPPXGXKXXXGGGGXG 446
GG G F G PPP P G G GG G
Sbjct: 188 GGFGGFGGGSGGPPPGPGGFGGFGGFGGEG 217
>SPAC23A1.17 |||WIP homolog|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1611
Score = 27.1 bits (57), Expect = 4.0
Identities = 18/49 (36%), Positives = 20/49 (40%), Gaps = 4/49 (8%)
Frame = +2
Query: 452 PPPPRXXFXXPGGGGGGXXXPQKXPP-PPPXXGXPXGGPP---PPXPXP 586
PP P+ P G P + PP P P G P PP PP P P
Sbjct: 1179 PPVPK-----PAAGVPPVPPPSEAPPVPKPSVGVPPVPPPSTAPPVPTP 1222
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 1,657,692
Number of Sequences: 5004
Number of extensions: 30133
Number of successful extensions: 340
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 53
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 497299314
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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