Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_K22
(899 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyce... 33 0.042
SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr 2|... 31 0.29
SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr 1||... 30 0.52
SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain pro... 29 0.90
SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr 1|... 29 0.90
SPBC646.09c |int6|yin6|translation initiation factor eIF3e|Schiz... 29 1.2
SPBC17D11.05 |tif32||translation initiation factor eIF3a|Schizos... 28 1.6
SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces pomb... 27 2.7
SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces pombe... 27 2.7
SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces po... 27 4.8
SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase Swr1|Schizo... 27 4.8
SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces pomb... 26 6.3
SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7 |S... 26 8.4
>SPCC188.04c |spc25||kinetochore protein Spc25|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 238
Score = 33.5 bits (73), Expect = 0.042
Identities = 25/98 (25%), Positives = 49/98 (50%), Gaps = 6/98 (6%)
Frame = +2
Query: 287 FSKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPD-- 460
F + ++ + +L N + + L + +A KA+++LEQ+ + E L K H +
Sbjct: 27 FDRFLQEERKKLLNNKNEYLRQLS-EINEAQKKAEKSLEQTEARKQNFTELLEKEHEEQA 85
Query: 461 -VEKNATALREKLQAAVQNTVQESQKL---AKKVSSKR 562
E+ + +EKL A ++ + S++L +SSKR
Sbjct: 86 ITEQEIFSFQEKLDAMLKRKQKLSEELDHYRAIISSKR 123
>SPBC16E9.16c |||sequence orphan|Schizosaccharomyces pombe|chr
2|||Manual
Length = 642
Score = 30.7 bits (66), Expect = 0.29
Identities = 25/68 (36%), Positives = 36/68 (52%), Gaps = 1/68 (1%)
Frame = +2
Query: 290 SKAWKDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRK-AHPDVE 466
++ + D S S +L A AK A DA A E +++ +IER+A K A + E
Sbjct: 129 ARQYNDRSTSEALELEARAKK---AAQDAE-LASERAREAQSSIERSASLREKQAREEAE 184
Query: 467 KNATALRE 490
+ ATALRE
Sbjct: 185 RAATALRE 192
>SPAC589.03c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 168
Score = 29.9 bits (64), Expect = 0.52
Identities = 13/46 (28%), Positives = 25/46 (54%)
Frame = +2
Query: 302 KDGSESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEE 439
KDG+++ +LN FAK L + + +A + + IE+++ E
Sbjct: 111 KDGTDAFANELNLFAKKLGFSKNSFDARALDTESEDETEIEKSSSE 156
>SPAC17C9.11c |||zinc finger protein, zf-C2H2 type/UBA domain
protein|Schizosaccharomyces pombe|chr 1|||Manual
Length = 240
Score = 29.1 bits (62), Expect = 0.90
Identities = 25/101 (24%), Positives = 49/101 (48%), Gaps = 1/101 (0%)
Frame = +2
Query: 392 EALEQSRQNIE-RTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSSKRAG 568
+ E++ + I+ R+ EEL++A + + A +EK + + + K+ +K + + A
Sbjct: 28 DQFEETEEEIKKRSPEELKQAIEALREKAKEKKEKERILALEEKKTNYKILQKSNDETAQ 87
Query: 569 D**ETGAQDQGRLRRLREEHPGGDQEDPGGRQRQAVSVDIE 691
QDQ RLR L++ Q+ QR+ + +IE
Sbjct: 88 A--MRKMQDQARLRDLQKIR---QQKAEDAEQRKKILAEIE 123
>SPAC12B10.10 |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 419
Score = 29.1 bits (62), Expect = 0.90
Identities = 16/76 (21%), Positives = 35/76 (46%)
Frame = +2
Query: 335 NAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQN 514
N + ++ AL + KA + LE+ ++ E + EE+ H + T+ + + +
Sbjct: 337 NLVSLAIYEALYEKFLKACKDLEEVSKSYEESREEIEALHETFTEEVTSFQSTKRLKEEK 396
Query: 515 TVQESQKLAKKVSSKR 562
+QE ++ K + R
Sbjct: 397 IIQEKSRVDKMIDEYR 412
>SPBC646.09c |int6|yin6|translation initiation factor
eIF3e|Schizosaccharomyces pombe|chr 2|||Manual
Length = 501
Score = 28.7 bits (61), Expect = 1.2
Identities = 18/54 (33%), Positives = 29/54 (53%), Gaps = 2/54 (3%)
Frame = +2
Query: 281 QDFSKAWKDGSESVLQQLNAFAKSLQGALGD-ANGKAKEALEQSR-QNIERTAE 436
+D + A+ + SVLQ+L+ + +QG LG N AL Q + QN++ E
Sbjct: 79 EDMANAFAEKRRSVLQELSELEEEVQGILGVLENPDLIAALRQDKGQNLQHLQE 132
>SPBC17D11.05 |tif32||translation initiation factor
eIF3a|Schizosaccharomyces pombe|chr 2|||Manual
Length = 932
Score = 28.3 bits (60), Expect = 1.6
Identities = 19/74 (25%), Positives = 37/74 (50%), Gaps = 1/74 (1%)
Frame = +2
Query: 347 KSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQE 526
++ + A +AN + E E ++ IE R+A ++ E+ + Q +E
Sbjct: 796 EAARAAEEEANRELHEQEEAQKRAIEERTRAAREAKEREQREMAEKLERQRRIQQERDEE 855
Query: 527 -SQKLAKKVSSKRA 565
S+KLA+K +++RA
Sbjct: 856 ISRKLAEKAAARRA 869
>SPBC30D10.07c |||biotin-protein ligase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 631
Score = 27.5 bits (58), Expect = 2.7
Identities = 10/20 (50%), Positives = 13/20 (65%)
Frame = -1
Query: 413 ASTVPKPPWPCRSRLRALPG 354
AST+ K PWP + L +PG
Sbjct: 38 ASTLEKEPWPASTALLVMPG 57
>SPBC9B6.08 |clc1||clathrin light chain|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 229
Score = 27.5 bits (58), Expect = 2.7
Identities = 19/66 (28%), Positives = 33/66 (50%)
Frame = +2
Query: 371 DANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKV 550
D + +E E S++ E E+ RKA D +N R+K+ + + +E +KL ++
Sbjct: 115 DQMKRIQERDESSKKLRESNIEKARKAIDDFYENFNDKRDKV---IAKSRKEQEKLLEEN 171
Query: 551 SSKRAG 568
SK G
Sbjct: 172 ESKSTG 177
>SPAC27D7.13c |ssm4|SPAC637.01c|p150-Glued|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 670
Score = 26.6 bits (56), Expect = 4.8
Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 5/57 (8%)
Frame = +2
Query: 350 SLQGALGDANGKAKEALEQSRQNIE-----RTAEELRKAHPDVEKNATALREKLQAA 505
S++ L + N + KE +E + RT +E EKN LRE+L+ A
Sbjct: 520 SMKDDLTEMNQRLKEQIESYENEVNSEITSRTLKEFETLKTQYEKNLCNLREQLKTA 576
>SPAC11E3.01c |swr1|SPAC2H10.03c|SNF2 family helicase
Swr1|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1288
Score = 26.6 bits (56), Expect = 4.8
Identities = 22/74 (29%), Positives = 32/74 (43%)
Frame = +2
Query: 311 SESVLQQLNAFAKSLQGALGDANGKAKEALEQSRQNIERTAEELRKAHPDVEKNATALRE 490
S + + A K L GA KAKE ++ R +RTA E+RK +E+ R
Sbjct: 143 SRRISGMILAHFKRLSGA---DEKKAKEEDKRIRLLAKRTAWEIRKKWKVIEREVRRRRA 199
Query: 491 KLQAAVQNTVQESQ 532
+ A Q + Q
Sbjct: 200 ERAAEAQRVAGKEQ 213
>SPBC29A10.13 |atp7||F0-ATPase subunit D|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 175
Score = 26.2 bits (55), Expect = 6.3
Identities = 10/24 (41%), Positives = 18/24 (75%)
Frame = +2
Query: 398 LEQSRQNIERTAEELRKAHPDVEK 469
+EQ+R E T E++++A P++EK
Sbjct: 126 IEQARPTEEITIEDMKQAVPEIEK 149
>SPBC16A3.19 |||histone acetyltransferase complex subunit Eaf7
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 272
Score = 25.8 bits (54), Expect = 8.4
Identities = 15/58 (25%), Positives = 33/58 (56%)
Frame = +2
Query: 383 KAKEALEQSRQNIERTAEELRKAHPDVEKNATALREKLQAAVQNTVQESQKLAKKVSS 556
K+++ LE S Q +E E + P+V++ +EK ++ V+ +E +K+++ + S
Sbjct: 132 KSEKPLETS-QKVEIETVETKPGEPEVKQETNLQKEKKESKVKLESKE-EKISRNLRS 187
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 2,558,255
Number of Sequences: 5004
Number of extensions: 38178
Number of successful extensions: 164
Number of sequences better than 10.0: 13
Number of HSP's better than 10.0 without gapping: 157
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 454497130
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -