Bombyx BLAST Result against spombe

BLASTX 2.2.12 [Aug-07-2005]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= MFBP10_F_I13
         (851 letters)

Database: spombe 
           5004 sequences; 2,362,478 total letters

Searching..................................................done

                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha 2|Schizosacchar...   233   2e-62
SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|ch...   229   5e-61
SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces...   114   1e-26
SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|...    98   2e-21
SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr 2|||...    26   5.9  
SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr...    26   7.8  

>SPBC800.05c |tub1|atb2, alp2, ban5|tubulin alpha
           2|Schizosaccharomyces pombe|chr 2|||Manual
          Length = 449

 Score =  233 bits (570), Expect = 2e-62
 Identities = 109/191 (57%), Positives = 135/191 (70%), Gaps = 1/191 (0%)
 Frame = +3

Query: 219 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 398
           CLEHGIQP+G M  +      D  F+TFFSETG GK+VPR+++VDLEP V+D+VRTG YR
Sbjct: 25  CLEHGIQPNGYMNPETASQNSDGGFSTFFSETGQGKYVPRSIYVDLEPNVIDQVRTGPYR 84

Query: 399 QLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXX 578
            LFHPEQLITGKEDA+NNYARGHYT+GKE+VD V D+IR++AD C+GLQGFL+FH     
Sbjct: 85  DLFHPEQLITGKEDASNNYARGHYTVGKELVDEVTDKIRRIADNCSGLQGFLVFHSFGGG 144

Query: 579 XXXXXXXLLMERLSVDYGKKSXLEFAIYPAPS-XFHCRRXXLQLYPHHXTXLXHSDCAFM 755
                  LL+ERL+++Y KKS L+F++YPAP            +   H T L  +DC FM
Sbjct: 145 TGSGFGALLLERLAMEYTKKSKLQFSVYPAPQVSTSVVEPYNSVLTTHAT-LDLADCTFM 203

Query: 756 VXXEXXYDICR 788
           V  E  YDICR
Sbjct: 204 VDNESCYDICR 214



 Score = 52.0 bits (119), Expect = 1e-07
 Identities = 21/24 (87%), Positives = 22/24 (91%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELY 217
           MRE IS+HVGQAG QIGNACWELY
Sbjct: 1   MREIISIHVGQAGTQIGNACWELY 24


>SPBC16A3.15c |nda2||tubulin alpha 1|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 455

 Score =  229 bits (559), Expect = 5e-61
 Identities = 110/196 (56%), Positives = 138/196 (70%), Gaps = 6/196 (3%)
 Frame = +3

Query: 219 CLEHGIQPDGQMPTD-----KTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVR 383
           CLEHGI PDG  PT+     K     +D F TFFSETG GK VPR+++VDLEP V+D+VR
Sbjct: 25  CLEHGIGPDG-FPTENSEVHKNNSYLNDGFGTFFSETGQGKFVPRSIYVDLEPNVIDQVR 83

Query: 384 TGTYRQLFHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFH 563
           TG Y+ LFHPEQ++TGKEDA+NNYARGHYT+GKE++D VL+RIR++AD C+GLQGFL+FH
Sbjct: 84  TGPYKDLFHPEQMVTGKEDASNNYARGHYTVGKEMIDSVLERIRRMADNCSGLQGFLVFH 143

Query: 564 XXXXXXXXXXXXLLMERLSVDYGKKSXLEFAIYPAPS-XFHCRRXXLQLYPHHXTXLXHS 740
                       LL+ERL+++YGKKS L+F++YPAP            +   H T L +S
Sbjct: 144 SFGGGTGSGLGALLLERLNMEYGKKSNLQFSVYPAPQVSTSVVEPYNSVLTTHAT-LDNS 202

Query: 741 DCAFMVXXEXXYDICR 788
           DC FMV  E  YDICR
Sbjct: 203 DCTFMVDNEACYDICR 218



 Score = 54.4 bits (125), Expect = 2e-08
 Identities = 27/44 (61%), Positives = 31/44 (70%), Gaps = 1/44 (2%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACWELY-LPGARHPA*WPDAHRQDHR 274
           MRE ISVHVGQAGVQIGNACWELY L     P  +P  + + H+
Sbjct: 1   MREVISVHVGQAGVQIGNACWELYCLEHGIGPDGFPTENSEVHK 44


>SPBC26H8.07c |nda3|ben1, alp12|tubulin beta |Schizosaccharomyces
           pombe|chr 2|||Manual
          Length = 448

 Score =  114 bits (275), Expect = 1e-26
 Identities = 56/149 (37%), Positives = 82/149 (55%)
 Frame = +3

Query: 225 EHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYRQL 404
           EHG+   G      T     +  N +F+E   GK+VPRAV VDLEP  +D V++G +  L
Sbjct: 27  EHGLDSAGIY--HGTSEAQHERLNVYFNEAAGGKYVPRAVLVDLEPGTMDAVKSGKFGNL 84

Query: 405 FHPEQLITGKEDAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXXXXXX 584
           F P+ +I G+  A N +A+GHYT G E+ D VLD +R+ A+ C  LQGF + H       
Sbjct: 85  FRPDNIIYGQSGAGNIWAKGHYTEGAELADAVLDVVRREAEACDALQGFQLTHSLGGGTG 144

Query: 585 XXXXXLLMERLSVDYGKKSXLEFAIYPAP 671
                LL+ ++  +Y  +    F++ PAP
Sbjct: 145 SGMGTLLLSKIREEYPDRMMATFSVAPAP 173



 Score = 27.5 bits (58), Expect = 2.6
 Identities = 10/21 (47%), Positives = 13/21 (61%)
 Frame = +2

Query: 146 MRECISVHVGQAGVQIGNACW 208
           MRE + +  GQ G Q+G A W
Sbjct: 1   MREIVHIQAGQCGNQVGAAFW 21


>SPBC32F12.04 |tug1|gtb1|gamma-tubulin|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 446

 Score = 97.9 bits (233), Expect = 2e-21
 Identities = 52/151 (34%), Positives = 84/151 (55%), Gaps = 2/151 (1%)
 Frame = +3

Query: 219 CLEHGIQPDGQMPTDKTIGGGDDSFNTFFSETGAGKHVPRAVFVDLEPTVVDEVRTGTYR 398
           CLEHGI PDG + +  T   G D  + FF ++   +++PRA+ +DLEP VV+ + + TY 
Sbjct: 26  CLEHGIGPDGTLESFAT--EGVDRKDVFFYQSDDTRYIPRAILIDLEPRVVNNILSDTYG 83

Query: 399 QLFHPEQLITGKE--DAANNYARGHYTIGKEIVDLVLDRIRKLADQCTGLQGFLIFHXXX 572
            L++PE ++  K    A NN+A G Y+  + I + ++D I + AD    L+GF + H   
Sbjct: 84  SLYNPENILITKNGGGAGNNWANG-YSHAERIFEDIMDMIDREADGSDSLEGFSLLHSIA 142

Query: 573 XXXXXXXXXLLMERLSVDYGKKSXLEFAIYP 665
                     L+ERL+  Y KK    ++++P
Sbjct: 143 GGTGSGLGSFLLERLNDRYPKKIIQTYSVFP 173


>SPBC215.13 |||sequence orphan|Schizosaccharomyces pombe|chr
           2|||Manual
          Length = 534

 Score = 26.2 bits (55), Expect = 5.9
 Identities = 16/38 (42%), Positives = 20/38 (52%)
 Frame = +1

Query: 217 LAWSTASSLMARCPQTRPSGVETILSTLSSARPELAST 330
           L  ST SSL +    ++PS   T  ST SSA P   S+
Sbjct: 170 LTSSTFSSLSSSTSSSQPSVSSTSSSTFSSAAPTSTSS 207


>SPCC645.06c |rgf3|lad1|RhoGEF Rgf3|Schizosaccharomyces pombe|chr
           3|||Manual
          Length = 1275

 Score = 25.8 bits (54), Expect = 7.8
 Identities = 13/22 (59%), Positives = 13/22 (59%)
 Frame = +2

Query: 245 WPDAHRQDHRGWRRFFQHFLQR 310
           W  A R D R  R  FQHFLQR
Sbjct: 590 WLAACRSDPRCRRLDFQHFLQR 611


  Database: spombe
    Posted date:  Oct 4, 2007 10:57 AM
  Number of letters in database: 2,362,478
  Number of sequences in database:  5004
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.279   0.0580    0.190 


Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,028,397
Number of Sequences: 5004
Number of extensions: 60753
Number of successful extensions: 168
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 155
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 163
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 422462090
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)