Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_G14
(840 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 234 1e-62
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 129 6e-31
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 128 7e-31
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 107 2e-24
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 76 7e-15
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 69 8e-13
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 68 2e-12
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 50 3e-07
SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces po... 49 9e-07
SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyce... 32 0.12
SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|ch... 28 1.9
SPAC29A4.20 |||RNA polymerase II elongator complex, histone acet... 27 2.5
SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces p... 26 5.8
SPBC1271.14 |||glutamate N-acetyltransferase |Schizosaccharomyce... 26 7.6
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 234 bits (573), Expect = 1e-62
Identities = 108/119 (90%), Positives = 111/119 (93%)
Frame = +1
Query: 277 PAIRXVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 456
P +MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA
Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97
Query: 457 PEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 633
PEEHP LLTEAPLNPK+NREKMTQI+FETFN PA YVAIQAVLSLYASGRTTGIVLDSG
Sbjct: 98 PEEHPCLLTEAPLNPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSG 156
Score = 135 bits (327), Expect = 6e-33
Identities = 62/69 (89%), Positives = 66/69 (95%)
Frame = +2
Query: 632 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTXEREIVRDIKEK 811
GDGV+HTVPIYEGYALPHAI+RLDLAGRDLTDYLMKIL ERGY+F+TT EREIVRDIKEK
Sbjct: 156 GDGVTHTVPIYEGYALPHAIMRLDLAGRDLTDYLMKILMERGYTFSTTAEREIVRDIKEK 215
Query: 812 LCYVALXFE 838
LCYVAL FE
Sbjct: 216 LCYVALDFE 224
Score = 70.1 bits (164), Expect = 4e-13
Identities = 30/36 (83%), Positives = 34/36 (94%)
Frame = +2
Query: 167 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 274
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPS G+
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGR 37
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 129 bits (311), Expect = 6e-31
Identities = 55/107 (51%), Positives = 82/107 (76%)
Frame = +1
Query: 313 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 492
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +LLTE P
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLTEPP 109
Query: 493 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 633
+NP ANREKM + MFE + +YVAIQAVLSLYA G ++G+V+DSG
Sbjct: 110 MNPVANREKMCETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSG 156
Score = 85.4 bits (202), Expect = 9e-18
Identities = 37/69 (53%), Positives = 49/69 (71%)
Frame = +2
Query: 632 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTXEREIVRDIKEK 811
GDGV+H VP+YE L H + RLD+AGRD T YL+ +L +GY+F T + E VR++KEK
Sbjct: 156 GDGVTHIVPVYESVVLNHLVGRLDVAGRDATRYLISLLLRKGYAFNRTADFETVREMKEK 215
Query: 812 LCYVALXFE 838
LCYV+ E
Sbjct: 216 LCYVSYDLE 224
Score = 39.1 bits (87), Expect = 8e-04
Identities = 17/34 (50%), Positives = 23/34 (67%)
Frame = +2
Query: 173 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 274
E A +V+DNG+G K G+A D+ PR FPS G+
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGR 35
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 128 bits (310), Expect = 7e-31
Identities = 60/115 (52%), Positives = 81/115 (70%), Gaps = 1/115 (0%)
Frame = +1
Query: 292 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNE-LRVAPEEH 468
VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + ++ L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 469 PVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 633
P+LLTE PL N+EK+ + +ET N PA+ ++Q VL+LYAS RTTGIVL+ G
Sbjct: 107 PLLLTEPPLANIRNKEKIAEYFYETLNVPALSFSLQPVLALYASARTTGIVLECG 161
Score = 83.8 bits (198), Expect = 3e-17
Identities = 35/68 (51%), Positives = 52/68 (76%)
Frame = +2
Query: 632 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTXEREIVRDIKEK 811
GDG++H+VPIY+G+++P AI + ++ GRD+TDYL L + G+ ++ E+EIVR+IKEK
Sbjct: 161 GDGLTHSVPIYDGFSIPSAIQQEEIGGRDVTDYLQLQLRKSGHELVSSAEKEIVREIKEK 220
Query: 812 LCYVALXF 835
CYVA F
Sbjct: 221 CCYVASDF 228
Score = 44.8 bits (101), Expect = 2e-05
Identities = 17/30 (56%), Positives = 23/30 (76%)
Frame = +2
Query: 185 LVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 274
+ +DNGSG KAGFAGDD P+ +FP+ G+
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGR 41
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 107 bits (257), Expect = 2e-24
Identities = 53/115 (46%), Positives = 71/115 (61%), Gaps = 9/115 (7%)
Frame = +1
Query: 316 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAP 492
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H LLTE P
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDHYFLLTEPP 132
Query: 493 LNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRT--------TGIVLDSG 633
LNP NRE +IMFE+FN +Y+A+QAVL+L AS + TG V+DSG
Sbjct: 133 LNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSG 187
Score = 52.0 bits (119), Expect = 1e-07
Identities = 25/64 (39%), Positives = 39/64 (60%)
Frame = +2
Query: 632 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTXEREIVRDIKEK 811
GDGV+H +P+ EGY + +I + LAGRD+T ++ +L +R ++ E IKE+
Sbjct: 187 GDGVTHIIPVAEGYVIGSSIKTMPLAGRDVTYFVQSLLRDRNEPDSSLKTAE---RIKEE 243
Query: 812 LCYV 823
CYV
Sbjct: 244 CCYV 247
Score = 38.3 bits (85), Expect = 0.001
Identities = 14/26 (53%), Positives = 21/26 (80%)
Frame = +2
Query: 185 LVVDNGSGMCKAGFAGDDAPRAVFPS 262
+++DNG+G K G+AG+DAP VFP+
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPT 33
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 75.8 bits (178), Expect = 7e-15
Identities = 35/92 (38%), Positives = 52/92 (56%)
Frame = +1
Query: 358 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLLTEAPLNPKANREKMTQIMF 537
+ +K I +G V NWD +W + +L+ P EHP+L+TE NP NR K + MF
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPILITEPFDNPPENRVKTLETMF 122
Query: 538 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 633
E+ PA Y+A Q + +ASG+ T ++D G
Sbjct: 123 ESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
Score = 39.9 bits (89), Expect = 4e-04
Identities = 16/35 (45%), Positives = 25/35 (71%)
Frame = +2
Query: 170 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRGK 274
+EV+A+V+D GS + GF+G+D P+ V PS G+
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCGE 43
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 68.9 bits (161), Expect = 8e-13
Identities = 35/113 (30%), Positives = 66/113 (58%), Gaps = 3/113 (2%)
Frame = +1
Query: 304 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVLL 480
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+ +
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPICM 120
Query: 481 TEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGR--TTGIVLDSG 633
TE NP R MT+++FE +N P++ I + S Y + + ++GIVL+ G
Sbjct: 121 TEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLG 173
Score = 30.7 bits (66), Expect = 0.27
Identities = 12/24 (50%), Positives = 17/24 (70%)
Frame = +2
Query: 185 LVVDNGSGMCKAGFAGDDAPRAVF 256
LV+DNGS +AG+ G+ P+ VF
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVF 50
Score = 27.9 bits (59), Expect = 1.9
Identities = 12/45 (26%), Positives = 21/45 (46%)
Frame = +2
Query: 632 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSF 766
G+ SH +P+ G + R+ G + YL+K+ + SF
Sbjct: 173 GNAASHVIPVLNGERILSEAKRISWGGSQSSSYLLKLFQIKYPSF 217
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 67.7 bits (158), Expect = 2e-12
Identities = 34/121 (28%), Positives = 65/121 (53%), Gaps = 2/121 (1%)
Frame = +1
Query: 277 PAIRXVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 450
P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 451 VAPEEHPVLLTEAPLNPKANREKMTQIMFETFNTPAMYVAIQAVLSLYASGRTTGIVLDS 630
V P E+ +++TE NP++ R+++ + FE + PA Y+ QAV +A+ ++T +++D
Sbjct: 97 VNPTEYAMMITEPSWNPQSVRQQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDI 156
Query: 631 G 633
G
Sbjct: 157 G 157
Score = 36.3 bits (80), Expect = 0.005
Identities = 14/34 (41%), Positives = 23/34 (67%)
Frame = +2
Query: 170 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSXRG 271
EE+ +LV+D GS + G+AG+++P + PS G
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYG 41
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 50.4 bits (115), Expect = 3e-07
Identities = 29/91 (31%), Positives = 44/91 (48%), Gaps = 4/91 (4%)
Frame = +1
Query: 373 PIEHGIVTNWDDMEKIWHHTFYNELRVAPEE----HPVLLTEAPLNPKANREKMTQIMFE 540
PI+ G V +W+ ++ W H Y+ L P + +PV L +RE TQ FE
Sbjct: 113 PIQRGRVVDWEALKAFWKH-LYSLLLKDPNDTTFRYPVCLVIPTYWSLYDRELATQFFFE 171
Query: 541 TFNTPAMYVAIQAVLSLYASGRTTGIVLDSG 633
P +A + ++ LYA G G+V+D G
Sbjct: 172 ECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202
>SPCC550.12 |arp6||actin-like protein Arp6|Schizosaccharomyces
pombe|chr 3|||Manual
Length = 401
Score = 48.8 bits (111), Expect = 9e-07
Identities = 23/69 (33%), Positives = 41/69 (59%)
Frame = +2
Query: 632 GDGVSHTVPIYEGYALPHAILRLDLAGRDLTDYLMKILTERGYSFTTTXEREIVRDIKEK 811
G +H +P+ + A+ R+D+ G+ LT+YL ++++ R Y+ E IV +IKE
Sbjct: 164 GYSFTHIIPVIDFSVQEQAVKRVDVGGKLLTNYLKEVISYRKYNM--MEETYIVNEIKES 221
Query: 812 LCYVALXFE 838
+CYV+ F+
Sbjct: 222 VCYVSQNFK 230
>SPAC26A3.05 |chc1||clathrin heavy chain Chc1 |Schizosaccharomyces
pombe|chr 1|||Manual
Length = 1666
Score = 31.9 bits (69), Expect = 0.12
Identities = 19/53 (35%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +1
Query: 433 FYNELRVAPEEHPVLLTE--APLNPKANREKMTQIMFETFNTPAM---YVAIQ 576
+Y L E+HP+LLT+ A L P+ + ++ +I ++ NTP + VAIQ
Sbjct: 1399 YYRALNFYLEQHPMLLTDLLAALTPRIDHPRVIRIFEKSENTPLILNFMVAIQ 1451
>SPAC24C9.06c |||aconitate hydratase|Schizosaccharomyces pombe|chr
1|||Manual
Length = 778
Score = 27.9 bits (59), Expect = 1.9
Identities = 10/27 (37%), Positives = 15/27 (55%)
Frame = +2
Query: 614 VSCWTPGDGVSHTVPIYEGYALPHAIL 694
+ W PG G+ H + + E YA P +L
Sbjct: 159 IGFWRPGSGIIHQI-VLENYAFPGGLL 184
>SPAC29A4.20 |||RNA polymerase II elongator complex, histone
acetyltransferase subunit |Schizosaccharomyces pombe|chr
1|||Manual
Length = 544
Score = 27.5 bits (58), Expect = 2.5
Identities = 19/70 (27%), Positives = 30/70 (42%), Gaps = 4/70 (5%)
Frame = +1
Query: 538 ETFNTPAMYVAIQAVLSLYASGRTTGIVLDSGRRCLPHRAHLRRIRTPPRHPASGLS--- 708
+ N A+ + I L S R T I+ + +R++R P ASG++
Sbjct: 29 KVINLNALKMRISKKHQLSESPRLTDIIAAIPPDAYLKESLMRKLRAKPVRTASGIAVVA 88
Query: 709 -RSRPHRLPH 735
+PHR PH
Sbjct: 89 VMCKPHRCPH 98
>SPBC115.01c |rrp46||exosome subunit Rrp46 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 226
Score = 26.2 bits (55), Expect = 5.8
Identities = 10/25 (40%), Positives = 18/25 (72%)
Frame = +1
Query: 475 LLTEAPLNPKANREKMTQIMFETFN 549
+L APL+ + +KM +++FET+N
Sbjct: 181 VLETAPLHAEEVSKKMKELLFETYN 205
>SPBC1271.14 |||glutamate N-acetyltransferase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 445
Score = 25.8 bits (54), Expect = 7.6
Identities = 9/15 (60%), Positives = 11/15 (73%)
Frame = -1
Query: 72 RPKLTSWNQNLKEFP 28
RP TSW +N +EFP
Sbjct: 129 RPHWTSWTENSEEFP 143
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,484,871
Number of Sequences: 5004
Number of extensions: 74776
Number of successful extensions: 245
Number of sequences better than 10.0: 14
Number of HSP's better than 10.0 without gapping: 212
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 241
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 414453330
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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