Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_E24
(924 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr 2||... 154 2e-38
SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex s... 85 1e-17
SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces pomb... 80 5e-16
SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomy... 58 1e-09
SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyce... 54 4e-08
SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit Arp42|Sc... 39 9e-04
SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces pombe... 36 0.006
SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit Arp9|Schizosa... 33 0.043
SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces po... 27 4.9
>SPBC32H8.12c |act1|cps8|actin |Schizosaccharomyces pombe|chr
2|||Manual
Length = 375
Score = 154 bits (373), Expect = 2e-38
Identities = 96/166 (57%), Positives = 106/166 (63%), Gaps = 10/166 (6%)
Frame = +1
Query: 166 DEEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQG-------RDGRYGTEGLLC 324
+EE+AALV+DNGSGMCKAGFAGDDAPRAVFPSIVGRPRH G +D G E
Sbjct: 2 EEEIAALVIDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHHGIMVGMGQKDSYVGDEAQSK 61
Query: 325 RR*GTEQKRYPDPQIPHRTRNRH*LG*HGEDLASYLLQ*AACRPRGTPS--PGSLX-APL 495
R G +YP I H N + +D+ R P P L APL
Sbjct: 62 R--GILTLKYP---IEHGIVN------NWDDMEKIWHHTFYNELRVAPEEHPCLLTEAPL 110
Query: 496 NPKANREKMTQIMFETFNTPAMYVXIQAVLSLYASGRTTGIVLDSG 633
NPK+NREKMTQI+FETFN PA YV IQAVLSLYASGRTTGIVLDSG
Sbjct: 111 NPKSNREKMTQIIFETFNAPAFYVAIQAVLSLYASGRTTGIVLDSG 156
Score = 137 bits (331), Expect = 2e-33
Identities = 60/67 (89%), Positives = 61/67 (91%)
Frame = +2
Query: 275 PAIRXVMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVA 454
P +MVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIV NWDDMEKIWHHTFYNELRVA
Sbjct: 38 PRHHGIMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVNNWDDMEKIWHHTFYNELRVA 97
Query: 455 PEEHPVL 475
PEEHP L
Sbjct: 98 PEEHPCL 104
Score = 38.7 bits (86), Expect = 0.001
Identities = 18/30 (60%), Positives = 18/30 (60%)
Frame = +3
Query: 657 PIYXGYALPHAIXXXXXXXXXXTDYXMKIL 746
PIY GYALPHAI TDY MKIL
Sbjct: 164 PIYEGYALPHAIMRLDLAGRDLTDYLMKIL 193
>SPAC11H11.06 |arp2|SPAC22F8.01|ARP2/3 actin-organizing complex
subunit Arp2|Schizosaccharomyces pombe|chr 1|||Manual
Length = 390
Score = 85.0 bits (201), Expect = 1e-17
Identities = 56/156 (35%), Positives = 79/156 (50%), Gaps = 2/156 (1%)
Frame = +1
Query: 172 EVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRP--RHQGRDGRYGTEGLLCRR*GTEQ 345
E A +V+DNG+G K G+A D+ PR FPSIVGRP R + + G + ++
Sbjct: 2 ESAPIVLDNGTGFVKVGYAKDNFPRFQFPSIVGRPILRAEEKTGNVQIKDVMVGDEAEAV 61
Query: 346 KRYPDPQIPHRTRNRH*LG*HGEDLASYLLQ*AACRPRGTPSPGSLXAPLNPKANREKMT 525
+ + P + + PRG + P+NP ANREKM
Sbjct: 62 RSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKILLT-EPPMNPVANREKMC 120
Query: 526 QIMFETFNTPAMYVXIQAVLSLYASGRTTGIVLDSG 633
+ MFE + +YV IQAVLSLYA G ++G+V+DSG
Sbjct: 121 ETMFERYGFGGVYVAIQAVLSLYAQGLSSGVVVDSG 156
Score = 61.3 bits (142), Expect = 2e-10
Identities = 22/55 (40%), Positives = 41/55 (74%)
Frame = +2
Query: 311 KDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL 475
KD VGDEA++ R +L +KYP+E+GI+ ++++M ++W +TF+ +L++ P +L
Sbjct: 50 KDVMVGDEAEAVRSLLQVKYPMENGIIRDFEEMNQLWDYTFFEKLKIDPRGRKIL 104
>SPBC1347.12 |||actin-like protein Arp1 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 379
Score = 79.8 bits (188), Expect = 5e-16
Identities = 57/162 (35%), Positives = 80/162 (49%), Gaps = 3/162 (1%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGRYGTEGLLCRR*GTEQKRYPDP 363
+ +DNGSG KAGFAGDD P+ +FP+ VGR +H+ + + G+E +
Sbjct: 12 ICIDNGSGFIKAGFAGDDIPKCLFPTCVGRIKHERVMPSSIQKDMFV---GSEAQNLRGL 68
Query: 364 QIPHRTRNRH*LG*HG--EDLASYLLQ*AACRPRGTPSPGSLXAP-LNPKANREKMTQIM 534
R R + E++ SY+ P L P L N+EK+ +
Sbjct: 69 LKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEHPLLLTEPPLANIRNKEKIAEYF 128
Query: 535 FETFNTPAMYVXIQAVLSLYASGRTTGIVLDSGRXCLPHRXP 660
+ET N PA+ +Q VL+LYAS RTTGIVL+ G L H P
Sbjct: 129 YETLNVPALSFSLQPVLALYASARTTGIVLECGDG-LTHSVP 169
Score = 72.5 bits (170), Expect = 8e-14
Identities = 32/63 (50%), Positives = 44/63 (69%), Gaps = 1/63 (1%)
Frame = +2
Query: 290 VMVGMGQKDSYVGDEAQSKRGILTLKYPIEHGIVTNWDDMEKIWHHTFYN-ELRVAPEEH 466
VM QKD +VG EAQ+ RG+L ++ PIE GI+ NW DME+IW + + + +L PEEH
Sbjct: 47 VMPSSIQKDMFVGSEAQNLRGLLKIQRPIERGIIQNWSDMEEIWSYIYSDQQLNTLPEEH 106
Query: 467 PVL 475
P+L
Sbjct: 107 PLL 109
>SPBP23A10.08 |alp5|arp4|actin-like protein Arp4|Schizosaccharomyces
pombe|chr 2|||Manual
Length = 433
Score = 58.4 bits (135), Expect = 1e-09
Identities = 50/159 (31%), Positives = 74/159 (46%), Gaps = 4/159 (2%)
Frame = +1
Query: 169 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPRHQGRDGR--YGTEGLLCRR*GTE 342
+EV+A+V+D GS + GF+G+D P+ V PS G + DGR +G E +
Sbjct: 9 DEVSAIVIDPGSKWTRIGFSGEDIPKCVLPSYCG----EFSDGRRLFGEEYI-------- 56
Query: 343 QKRYPDPQIPHRTRNRH*LG*H-GEDLASYLLQ*AACRPRGTPSPGSLXAPL-NPKANRE 516
K P +I + RN DL Y L+ + P + P NP NR
Sbjct: 57 YKSNPGMEIKNAIRNGWVENWDVTVDLWRYGLE-QQLKTNPLEHPILITEPFDNPPENRV 115
Query: 517 KMTQIMFETFNTPAMYVXIQAVLSLYASGRTTGIVLDSG 633
K + MFE+ PA Y+ Q + +ASG+ T ++D G
Sbjct: 116 KTLETMFESLRCPATYLAKQETCAAFASGKGTACLVDIG 154
Score = 36.3 bits (80), Expect = 0.006
Identities = 14/40 (35%), Positives = 22/40 (55%)
Frame = +2
Query: 356 LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPVL 475
+ +K I +G V NWD +W + +L+ P EHP+L
Sbjct: 63 MEIKNAIRNGWVENWDVTVDLWRYGLEQQLKTNPLEHPIL 102
>SPAC630.03 |arp3|act2|actin-like protein Arp3|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 427
Score = 53.6 bits (123), Expect = 4e-08
Identities = 21/52 (40%), Positives = 31/52 (59%), Gaps = 1/52 (1%)
Frame = +2
Query: 314 DSYVGDEAQSKRGI-LTLKYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEH 466
D ++G++A K +L YPI HG + NWD ME+ W + + LR PE+H
Sbjct: 73 DFFIGNDALKKASAGYSLDYPIRHGQIENWDHMERFWQQSLFKYLRCEPEDH 124
Score = 50.8 bits (116), Expect = 3e-07
Identities = 27/56 (48%), Positives = 35/56 (62%), Gaps = 8/56 (14%)
Frame = +1
Query: 490 PLNPKANREKMTQIMFETFNTPAMYVXIQAVLSLYASGRT--------TGIVLDSG 633
PLNP NRE +IMFE+FN +Y+ +QAVL+L AS + TG V+DSG
Sbjct: 132 PLNPPENRENTAEIMFESFNCAGLYIAVQAVLALAASWTSSKVTDRSLTGTVVDSG 187
Score = 40.7 bits (91), Expect = 3e-04
Identities = 14/28 (50%), Positives = 23/28 (82%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSIV 267
+++DNG+G K G+AG+DAP VFP+++
Sbjct: 8 IIMDNGTGYSKLGYAGNDAPSYVFPTVI 35
>SPAC23D3.09 |arp42|arp4|SWI/SNF and RSC complex subunit
Arp42|Schizosaccharomyces pombe|chr 1|||Manual
Length = 430
Score = 39.1 bits (87), Expect = 9e-04
Identities = 41/160 (25%), Positives = 74/160 (46%), Gaps = 5/160 (3%)
Frame = +1
Query: 169 EEVAALVVDNGSGMCKAGFAGDDAPRAVFPSIVG-RPRHQGRDGRYGTEGLLCRR*GTEQ 345
EE+ +LV+D GS + G+AG+++P + PS G R GR+ +Y + L Q
Sbjct: 8 EEIPSLVIDPGSCWTRFGYAGEESPMTILPSYYGVRSDVTGRN-KYVVDEL--------Q 58
Query: 346 KRYPDPQIPHRTRNRH*LG*HGEDLASYLLQ*AACRPRGTPSPGSLXAPLNPKANREKM- 522
P P + + + + E + + + P+ ++ P N + +
Sbjct: 59 IHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQVNPTEYAMMI-TEPSWNPQSVR 117
Query: 523 TQIM---FETFNTPAMYVXIQAVLSLYASGRTTGIVLDSG 633
QIM FE + PA Y+ QAV +A+ ++T +++D G
Sbjct: 118 QQIMEAAFEQLHVPAFYLTKQAVCVAFANSKSTALIVDIG 157
Score = 29.1 bits (62), Expect = 0.93
Identities = 17/69 (24%), Positives = 32/69 (46%), Gaps = 2/69 (2%)
Frame = +2
Query: 275 PAIRXVMVGMGQKDSYVGDEAQSKRGI--LTLKYPIEHGIVTNWDDMEKIWHHTFYNELR 448
P+ V + ++ YV DE Q I + +K +GI+ +W+ W +L+
Sbjct: 37 PSYYGVRSDVTGRNKYVVDELQIHAPIPGMEVKNGKSNGIIQDWESTLYTWERGLKEKLQ 96
Query: 449 VAPEEHPVL 475
V P E+ ++
Sbjct: 97 VNPTEYAMM 105
>SPBC365.10 |||actin-like protein Arp5 |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 721
Score = 36.3 bits (80), Expect = 0.006
Identities = 20/57 (35%), Positives = 33/57 (57%), Gaps = 3/57 (5%)
Frame = +1
Query: 472 PGSLXAPL-NPKANREKMTQIMFETFNTPAMYVXIQAVLSLYASGR--TTGIVLDSG 633
P + PL NP R MT+++FE +N P++ I + S Y + + ++GIVL+ G
Sbjct: 117 PICMTEPLANPTYVRSTMTELLFELYNAPSVAYGIDGLFSFYHNTKPSSSGIVLNLG 173
Score = 35.9 bits (79), Expect = 0.008
Identities = 15/32 (46%), Positives = 22/32 (68%)
Frame = +1
Query: 184 LVVDNGSGMCKAGFAGDDAPRAVFPSIVGRPR 279
LV+DNGS +AG+ G+ P+ VF ++V R R
Sbjct: 27 LVIDNGSWQLRAGWGGEKDPKLVFDNLVSRYR 58
Score = 34.7 bits (76), Expect = 0.019
Identities = 16/58 (27%), Positives = 34/58 (58%), Gaps = 1/58 (1%)
Frame = +2
Query: 302 MGQKDSYVGDEAQSKRGILTL-KYPIEHGIVTNWDDMEKIWHHTFYNELRVAPEEHPV 472
+ + + VG++ + G ++ + P E +++NWD ME++ +TF +L + EHP+
Sbjct: 62 LSRTSTLVGNDTLIEVGSRSIARSPFERNVISNWDLMEQVLDYTFL-KLGIDRMEHPI 118
>SPAC1071.06 |arp9||SWI/SNF and RSC complex subunit
Arp9|Schizosaccharomyces pombe|chr 1|||Manual
Length = 523
Score = 33.5 bits (73), Expect = 0.043
Identities = 15/42 (35%), Positives = 22/42 (52%)
Frame = +1
Query: 508 NREKMTQIMFETFNTPAMYVXIQAVLSLYASGRTTGIVLDSG 633
+RE TQ FE P + + ++ LYA G G+V+D G
Sbjct: 161 DRELATQFFFEECQVPGFTIAYEPLMGLYAIGILHGLVIDIG 202
Score = 25.8 bits (54), Expect = 8.6
Identities = 7/21 (33%), Positives = 13/21 (61%)
Frame = +2
Query: 371 PIEHGIVTNWDDMEKIWHHTF 433
PI+ G V +W+ ++ W H +
Sbjct: 113 PIQRGRVVDWEALKAFWKHLY 133
>SPAC17A2.12 |||ATP-dependent DNA helicase|Schizosaccharomyces
pombe|chr 1|||Manual
Length = 897
Score = 26.6 bits (56), Expect = 4.9
Identities = 17/65 (26%), Positives = 23/65 (35%)
Frame = +2
Query: 539 KHSTRPPCTSPSKPCSRCTRPVVPPVSCWTPGXGVSHTVXHLXRIRTPPRHPASGLXRSR 718
+H T P +K SR P+ PP S P +S + P H L +
Sbjct: 162 EHLTPPSSFITAKQLSRLPTPLPPPSSSSLPTGTISTNSFCPYERKVQPEHVTKELHQLL 221
Query: 719 PHRLP 733
H P
Sbjct: 222 QHNTP 226
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,393,382
Number of Sequences: 5004
Number of extensions: 66659
Number of successful extensions: 193
Number of sequences better than 10.0: 9
Number of HSP's better than 10.0 without gapping: 161
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 192
length of database: 2,362,478
effective HSP length: 73
effective length of database: 1,997,186
effective search space used: 467341524
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
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