Last updated: 2022/11/18
BLASTX 2.2.12 [Aug-07-2005]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= MFBP10_F_E03
(889 letters)
Database: spombe
5004 sequences; 2,362,478 total letters
Searching..................................................done
Score E
Sequences producing significant alignments: (bits) Value
SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces p... 107 2e-24
SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase ... 59 1e-09
SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase... 45 2e-05
SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces pombe... 43 5e-05
SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde d... 38 0.003
SPCC13B11.01 |adh1|adh|alcohol dehydrogenase Adh1|Schizosaccharo... 37 0.003
SPBC16A3.02c |||mitochondrial peptidase |Schizosaccharomyces pom... 32 0.095
SPAC27E2.06c |||methionine-tRNA ligase, mitochondrial|Schizosacc... 28 1.5
SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr 1... 27 4.7
SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family ... 26 6.2
SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit Prp1|... 26 8.2
>SPBC1773.05c |tms1||hexitol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 360
Score = 107 bits (257), Expect = 2e-24
Identities = 57/137 (41%), Positives = 74/137 (54%), Gaps = 1/137 (0%)
Frame = +3
Query: 348 PACRVGTASSVRQDDTTYALXMIFCATPPVHGNLVRYYKHAADFCFKLPDHVTMEEGALL 527
P C R M F ATPP G L YY DFC KLP +++EEGAL
Sbjct: 93 PGCVCRLCDYCRSGRYNLCPHMEFAATPPYDGTLRTYYITTEDFCTKLPKQISVEEGALF 152
Query: 528 EPLAVGIHACKRGGVSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILXSRLDFA-K 704
EP++V +HA RG + G VLV+G G +GLL M AKA+GA ++ +D SR++FA K
Sbjct: 153 EPMSVAVHAMTRGNLKCGSRVLVMGCGTVGLLMMAVAKAYGAIDIVAVDASPSRVEFAQK 212
Query: 705 SLGADYTLLIGKDSNEA 755
+GA I NE+
Sbjct: 213 YVGAKPFTPIAAKENES 229
Score = 62.1 bits (144), Expect = 1e-10
Identities = 28/70 (40%), Positives = 44/70 (62%), Gaps = 1/70 (1%)
Frame = +1
Query: 73 MATDNLTALLYKPNDLRLVQTPIPEISED-EVLLRMDCVGICGSDVHYWQKGQCGHFVLE 249
MA +L K D + P +++D +V + + GICGSDVHYW++G G F+L+
Sbjct: 1 MAPAEKAFVLRKKMDTAIEDRPGQTLTDDHQVKVAIKATGICGSDVHYWKEGGIGDFILK 60
Query: 250 EPMIMGHEAS 279
+PMI+GHE++
Sbjct: 61 KPMILGHESA 70
Score = 59.3 bits (137), Expect = 7e-10
Identities = 23/50 (46%), Positives = 33/50 (66%)
Frame = +2
Query: 284 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLCPXHDLLRHAP 433
VV ++G V +L GD VA+EPG CR C++C++GRY+LCP + P
Sbjct: 72 VVVEVGKGVSSLKPGDPVAVEPGCVCRLCDYCRSGRYNLCPHMEFAATPP 121
>SPBC1198.01 |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 423
Score = 58.8 bits (136), Expect = 1e-09
Identities = 29/76 (38%), Positives = 42/76 (55%), Gaps = 1/76 (1%)
Frame = +3
Query: 480 CFKLPDHVTMEEGALL-EPLAVGIHACKRGGVSAGHVVLVLGAGPIGLLTMLTAKAFGAH 656
C KLPD + EG + + L +HAC G V G V + G GPIGL A+ GA
Sbjct: 188 CCKLPDDIPDSEGLFMSDVLCTSLHACTLGEVKKGDTVAIWGMGPIGLYAGRWAQILGAS 247
Query: 657 KVLIIDILXSRLDFAK 704
KV+ I+++ R++ A+
Sbjct: 248 KVIGIEVVPERIELAR 263
Score = 42.7 bits (96), Expect = 7e-05
Identities = 18/40 (45%), Positives = 23/40 (57%)
Frame = +2
Query: 284 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 403
+VA+ G +V NL +GDRV I + C C FCK Y C
Sbjct: 102 IVAEKGDEVNNLEIGDRVVIAFDLACGQCSFCKRHEYAAC 141
>SPBC1539.07c |||glutathione-dependent formaldehyde dehydrogenase
|Schizosaccharomyces pombe|chr 2|||Manual
Length = 378
Score = 44.8 bits (101), Expect = 2e-05
Identities = 30/84 (35%), Positives = 40/84 (47%)
Frame = +3
Query: 570 VSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILXSRLDFAKSLGADYTLLIGKDSN 749
V +G V V+G G +GL M A A GA +++ IDI + +AK GA + S
Sbjct: 191 VESGSTVAVVGCGCVGLAAMQGAVAAGASRIIAIDINADKEVYAKKFGATDFI---DSSK 247
Query: 750 EADLVXEXX*XTGGTPDVSFDAXG 821
DLV T G D +FD G
Sbjct: 248 VKDLVQYVIDVTDGGVDYAFDCTG 271
Score = 37.1 bits (82), Expect = 0.003
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +2
Query: 284 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 403
+V IG V N+ GD V + C+ C+FC++G+ +LC
Sbjct: 74 IVESIGEGVINVRPGDHVILLYTPECKECKFCRSGKTNLC 113
>SPBC1773.06c |||alcohol dehydrogenase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 346
Score = 43.2 bits (97), Expect = 5e-05
Identities = 30/83 (36%), Positives = 43/83 (51%), Gaps = 4/83 (4%)
Frame = +3
Query: 492 PDHVTMEEGALLEPLAV----GIHACKRGGVSAGHVVLVLGAGPIGLLTMLTAKAFGAHK 659
P +++ EE A L AV G+ K V G+ VLVLG G + + A A GA+
Sbjct: 133 PKNLSFEEIATLPCAAVTAWNGLFGSKEHQVKPGNNVLVLGTGGVSTFALQFALAAGAN- 191
Query: 660 VLIIDILXSRLDFAKSLGADYTL 728
V + +L+FAK LGA +T+
Sbjct: 192 VTVTSSSDEKLEFAKKLGATHTI 214
>SPCC13B11.04c ||SPCC777.01c|glutathione-dependent formaldehyde
dehydrogenase|Schizosaccharomyces pombe|chr 3|||Manual
Length = 380
Score = 37.5 bits (83), Expect = 0.003
Identities = 15/40 (37%), Positives = 24/40 (60%)
Frame = +2
Query: 284 VVAKIGSKVKNLTVGDRVAIEPGVPCRYCEFCKTGRYHLC 403
+V +G +V + VGD V C+ C+FCK+G+ +LC
Sbjct: 77 IVESVGPQVTTVQVGDPVIALYTPECKTCKFCKSGKTNLC 116
Score = 30.3 bits (65), Expect = 0.38
Identities = 23/84 (27%), Positives = 37/84 (44%)
Frame = +3
Query: 570 VSAGHVVLVLGAGPIGLLTMLTAKAFGAHKVLIIDILXSRLDFAKSLGADYTLLIGKDSN 749
+ G V V G G +GL + A A ++ ID+ + ++A S GA T I +
Sbjct: 194 IKEGDSVAVFGLGSVGLAVIQGAVKKRAGRIFGIDVNPEKKNWAMSFGA--TDFINPNDL 251
Query: 750 EADLVXEXX*XTGGTPDVSFDAXG 821
++ + T G D +FD G
Sbjct: 252 QSPIQDVLIHETDGGLDWTFDCTG 275
>SPCC13B11.01 |adh1|adh|alcohol dehydrogenase
Adh1|Schizosaccharomyces pombe|chr 3|||Manual
Length = 350
Score = 37.1 bits (82), Expect = 0.003
Identities = 19/59 (32%), Positives = 34/59 (57%), Gaps = 2/59 (3%)
Frame = +1
Query: 109 PNDLRLVQTPIPEISEDEVLLRMDCVGICGSDVHYWQKGQCGHFVL--EEPMIMGHEAS 279
P +++ + P+ E +DEVL+ + G+C +D+H Q G + L + P+I GHE +
Sbjct: 18 PENVKFEEVPVAEPGQDEVLVNIKYTGVCHTDLHALQ----GDWPLPAKMPLIGGHEGA 72
Score = 37.1 bits (82), Expect = 0.003
Identities = 16/42 (38%), Positives = 23/42 (54%), Gaps = 1/42 (2%)
Frame = +2
Query: 284 VVAKIGSKVKNLTVGDRVAIE-PGVPCRYCEFCKTGRYHLCP 406
VV K+G+ V L +GDRV ++ C CE+C +CP
Sbjct: 74 VVVKVGAGVTRLKIGDRVGVKWMNSSCGNCEYCMKAEETICP 115
>SPBC16A3.02c |||mitochondrial peptidase |Schizosaccharomyces
pombe|chr 2|||Manual
Length = 347
Score = 32.3 bits (70), Expect = 0.095
Identities = 29/89 (32%), Positives = 40/89 (44%), Gaps = 3/89 (3%)
Frame = +3
Query: 489 LPDHVTMEEGAL--LEPLAVGIHACKRGGVSAGHVVLVLGA-GPIGLLTMLTAKAFGAHK 659
LPD V+ EGA + L ++ V G +++ GA G +G + AKA
Sbjct: 134 LPDGVSFNEGAGFGIAGLTAWEVLVRQMKVKPGTKLVIEGASGGVGTFAVALAKALECEV 193
Query: 660 VLIIDILXSRLDFAKSLGADYTLLIGKDS 746
I LD KSLGA +TL KD+
Sbjct: 194 TTISST--ENLDLCKSLGATHTLDYKKDN 220
>SPAC27E2.06c |||methionine-tRNA ligase,
mitochondrial|Schizosaccharomyces pombe|chr 1|||Manual
Length = 539
Score = 28.3 bits (60), Expect = 1.5
Identities = 21/74 (28%), Positives = 33/74 (44%), Gaps = 1/74 (1%)
Frame = -3
Query: 647 KGFSCEHGKETD-GSRSQYQHHVSRRDSAPLARVNAHREGLQ*RSFFHGHVIRKLEAKIS 471
K S E GKE S Y +S+ S + N + +Q S FH V+ +L+ IS
Sbjct: 168 KRVSMETGKEVQWSSEMNYHFLLSKFQSRLIEHYNKNPNFVQ-PSIFHTQVLEELKTGIS 226
Query: 470 CVLVISDEVSVYWG 429
+ + + + WG
Sbjct: 227 DLSISRPKQRLSWG 240
>SPAPB18E9.04c |||sequence orphan|Schizosaccharomyces pombe|chr
1|||Manual
Length = 800
Score = 26.6 bits (56), Expect = 4.7
Identities = 15/39 (38%), Positives = 17/39 (43%), Gaps = 2/39 (5%)
Frame = -1
Query: 673 SIMSTLCAPKALAVSMVRRPMGPAPSTSTTCPAET--PP 563
S ST P + S P PSTST+C T PP
Sbjct: 309 STSSTSTPPPPASTSSTGTSSSPLPSTSTSCTTSTSIPP 347
>SPAC16E8.01 |||cytoskeletal protein binding protein Sla1 family
|Schizosaccharomyces pombe|chr 1|||Manual
Length = 1420
Score = 26.2 bits (55), Expect = 6.2
Identities = 15/34 (44%), Positives = 17/34 (50%)
Frame = +1
Query: 391 IPPMPXT*SSAPRPQYTETSSDITSTQLIFASSF 492
IPP P T S PRP T S+ T T A S+
Sbjct: 272 IPPPPATPSQPPRPP-TNASTRSTGTSSSMAHSY 304
>SPBC6B1.07 |prp1|zer1|U4/U6 x U5 tri-snRNP complex subunit
Prp1|Schizosaccharomyces pombe|chr 2|||Manual
Length = 906
Score = 25.8 bits (54), Expect = 8.2
Identities = 6/32 (18%), Positives = 14/32 (43%)
Frame = +1
Query: 508 WKKERYWSPSRWAFTRARGAESRRDTWCWYWE 603
W +++ W + + D WCW+++
Sbjct: 821 WLEKKADKARSWFLKAVKADQDNGDVWCWFYK 852
Database: spombe
Posted date: Oct 4, 2007 10:57 AM
Number of letters in database: 2,362,478
Number of sequences in database: 5004
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.279 0.0580 0.190
Matrix: BLOSUM62
Gap Penalties: Existence: 9, Extension: 2
Number of Hits to DB: 3,373,557
Number of Sequences: 5004
Number of extensions: 66608
Number of successful extensions: 196
Number of sequences better than 10.0: 11
Number of HSP's better than 10.0 without gapping: 172
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 194
length of database: 2,362,478
effective HSP length: 72
effective length of database: 2,002,190
effective search space used: 446488370
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 37 (14.9 bits)
X3: 62 (25.0 bits)
S1: 41 (21.7 bits)
- SilkBase 1999-2023 -